Characterization of p75 neurotrophin receptor expression in human dental pulp stem cells

2016 ◽  
Vol 53 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Wenru Pan ◽  
Karlea L. Kremer ◽  
Xenia Kaidonis ◽  
Victoria E. Ludlow ◽  
Mary‐Louise Rogers ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Receptor Expression ◽  
Neurotrophin Receptor ◽  
P75 Neurotrophin Receptor ◽  
Human Dental Pulp

Preparation and characterization of titanium—segmented polyurethane composites for bone tissue engineering

2018 ◽  
Vol 33 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Fernando Javier Aguilar-Perez ◽  
Rossana Vargas-Coronado ◽  
Jose Manuel Cervantes-Uc ◽  
Juan Valerio Cauich-Rodriguez ◽  
Raul Rosales-Ibañez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Soft Segment ◽  
Physicochemical Characterization ◽  
Dental Pulp Stem Cells ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Human Dental Pulp ◽  
Polyurethane Composites

Segmented polyurethanes were prepared with polycaprolactone diol as soft segment and 4,4-methylene-bis cyclohexyl diisocyanate and l-glutamine as the rigid segment. These polyurethanes were filled with 1 wt.% to 5 wt.% titanium particles (Ti), physicochemically characterized and their biocompatibility assessed using human dental pulp stem cells and mice osteoblasts. Physicochemical characterization showed that composites retained the properties of the semicrystalline polyurethane as they exhibited a glass transition temperature (Tg) between −35°C and −45°C, melting temperature (Tm) at 52°C and crystallinity close to 40% as determined by differential scanning calorimetry. In agreement with this, X-ray diffraction showed reflections at 21.3° and 23.6° for polycaprolactone diol and reflections at 35.1°, 38.4°, and 40.2° for Ti particles suggesting that these particles are not acting as nucleating sites. The addition of up to 5 wt.% of Ti reduced both, tensile strength and maximum strain from 1.9 MPa to 1.2 MPa, and from 670% to 172% for pristine and filled polyurethane, respectively. Although there were differences between composites at low strain rates, no significant differences in mechanical behavior were observed at higher strain rate where a tensile stress of 8.5 MPa and strain of 223% were observed for 5 wt.% composites. The addition to titanium particles had a beneficial effect on both human dental pulp stem cells and osteoblasts viability, as it increased with the amount of titanium in composites up to 10 days of incubation.

scholarly journals Purinergic Signaling Modulates Survival/Proliferation of Human Dental Pulp Stem Cells

2018 ◽  
Vol 98 (2) ◽  
pp. 242-249 ◽  
Author(s):  
S. Zhang ◽  
D. Ye ◽  
L. Ma ◽  
Y. Ren ◽  
R.T. Dirksen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Purinergic Receptor ◽  
Purinergic Signaling ◽  
P2x Receptors ◽  
Dental Pulp Stem Cells ◽  
Receptor Expression ◽  
Receptor Antagonists ◽  
Inward Currents ◽  
Human Dental Pulp

Human dental pulp stem cells (hDPSCs) reside in postnatal dental pulp and exhibit the potential to differentiate into odontoblasts as well as neurons. However, the intercellular signaling niches necessary for hDPSC survival and self-renewal remain largely unknown. The objective of this study is to demonstrate the existence of intercellular purinergic signaling in hDPSCs and to assess the impact of purinergic signaling on hDPSC survival and proliferation. hDPSCs were isolated from extracted third molars and cultured in minimum essential medium. To demonstrate responsiveness to ATP application and inhibitions by purinergic receptor antagonists, whole cell patch-clamp recordings of ATP-induced currents were recorded from cultured hDPSCs. Immunofluorescence and enzymatic histochemistry staining were performed to assess purinergic receptor expression and ectonucleotidase activity in hDPSCs, respectively. To determine the effects of purinergic signaling on hDPSC, purinergic receptor antagonists and an ectonucleotidase inhibitor were applied in culture medium, and hDPSC survival and proliferation were assessed with DAPI staining and Ki67 immunofluorescence staining, respectively. We demonstrated that ATP application induced inward currents in hDPSCs. P2X and P2Y receptors are involved in the generation of ATP-induced inward currents. We also detected expression of NTPDase3 and ectonucleotidase activity in hDPSCs. We further demonstrated that purinergic receptors were tonically activated in hDPSCs and that inhibition of ectonucleotidase activity enhanced ATP-induced inward currents. Furthermore, we found that blocking P2Y and P2X receptors reduced—and inhibition of ecto-ATPase activity enhanced—the survival and proliferation of hDPSCs, while blocking P2X receptors alone affected only hDPSC proliferation. Autocrine/paracrine purinergic signaling is essential for hDPSC survival and proliferation. These results reveal potential targets to manipulate hDPSCs to promote tooth/dental pulp repair and regeneration.

Characterization of Dental Pulp Stem Cells of Human Tooth Germs

2008 ◽  
Vol 87 (7) ◽  
pp. 676-681 ◽  
Author(s):  
T. Takeda ◽  
Y. Tezuka ◽  
M. Horiuchi ◽  
K. Hosono ◽  
K. Iida ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Tooth ◽  
Dna Array ◽  
Human Dental Pulp ◽  
Tooth Germs

In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a higher proliferation rate than those isolated at a later stage. When the cells from either group were cultured in medium promoting differentiation toward cells of the osteo/odontoblastic lineage, both became alkaline-phosphatase-positive, produced calcified matrix, and were also capable of forming dentin-like matrix on scaffolds in vivo. However, during long-term passage, these cells underwent a change in morphology and lost their differentiation ability. The results of a DNA array experiment showed that the expression of several genes, such as WNT16, was markedly changed with an increasing number of passages, which might have caused the loss of their characteristics as hDPSCs.

Functional and molecular characterization of transmembrane intracellular pH regulators in human dental pulp stem cells

2018 ◽  
Vol 90 ◽  
pp. 19-26 ◽  
Author(s):  
Gunng-Shinng Chen ◽  
Shiao-Pieng Lee ◽  
Shu-Fu Huang ◽  
Shih-Chi Chao ◽  
Chung-Yi Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Molecular Characterization ◽  
Intracellular Ph ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Characterization of SSEA-3 Positive Cells Derived from Human Dental Pulp Stem Cells

2019 ◽  
Vol 28 (4) ◽  
pp. 335-340
Author(s):  
Takatoshi Nagano ◽  
Taichiro Funatsu ◽  
Kazuhiro Gomi
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Chrysin induces osteogenic differentiation of human dental pulp stem cells

2021 ◽  
Vol 400 (2) ◽  
pp. 112466
Author(s):  
J.F. Huo ◽  
M.L. Zhang ◽  
X.X. Wang ◽  
D.H. Zou
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp
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