scholarly journals Evaluation of Strategies for the Decontamination of Equipment for Geomyces destructans, the Causative Agent of the White-Nose Syndrome (WNS)

2013 ◽  
pp. 1-10 ◽  
Author(s):  
Virginia Shelley ◽  
Samantha Kaiser ◽  
Elizabeth Shelley ◽  
Tim Williams ◽  
Marcelo Kramer ◽  
...  
Keyword(s):  
Causative Agent ◽  
Geomyces Destructans ◽  
White Nose Syndrome

scholarly journals Bacteria Isolated from Bats Inhibit the Growth of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0121329 ◽  
Author(s):  
Joseph R. Hoyt ◽  
Tina L. Cheng ◽  
Kate E. Langwig ◽  
Mallory M. Hee ◽  
Winifred F. Frick ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome

scholarly journals Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome

2015 ◽  
Vol 112 (24) ◽  
pp. 7478-7483 ◽  
Author(s):  
Anthony J. O’Donoghue ◽  
Giselle M. Knudsen ◽  
Chapman Beekman ◽  
Jenna A. Perry ◽  
Alexander D. Johnson ◽  
...  
Keyword(s):  
Causative Agent ◽  
Genetic Manipulation ◽  
Hydrolytic Enzymes ◽  
Collagen Degradation ◽  
Mammalian Host ◽  
Peptidase Activity ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome ◽  
Proteolytic Activities ◽  
The Impact

Pseudogymnoascus destructans is the causative agent of white-nose syndrome, a disease that has caused the deaths of millions of bats in North America. This psychrophilic fungus proliferates at low temperatures and targets hibernating bats, resulting in their premature arousal from stupor with catastrophic consequences. Despite the impact of white-nose syndrome, little is known about the fungus itself or how it infects its mammalian host. P. destructans is not amenable to genetic manipulation, and therefore understanding the proteins involved in infection requires alternative approaches. Here, we identify hydrolytic enzymes secreted by P. destructans, and use a novel and unbiased substrate profiling technique to define active peptidases. These experiments revealed that endopeptidases are the major proteolytic activities secreted by P. destructans, and that collagen, the major structural protein in mammals, is actively degraded by the secretome. A serine endopeptidase, hereby-named Destructin-1, was subsequently identified, and a recombinant form overexpressed and purified. Biochemical analysis of Destructin-1 showed that it mediated collagen degradation, and a potent inhibitor of peptidase activity was identified. Treatment of P. destructans-conditioned media with this antagonist blocked collagen degradation and facilitated the detection of additional secreted proteolytic activities, including aminopeptidases and carboxypeptidases. These results provide molecular insights into the secretome of P. destructans, and identify serine endopeptidases that have the clear potential to facilitate tissue invasion and pathogenesis in the mammalian host.

A review of factors affecting cave climates for hibernating bats in temperate North America

2013 ◽  
Vol 21 (1) ◽  
pp. 28-39 ◽  
Author(s):  
Roger W. Perry
Keyword(s):  
North America ◽  
Energy Savings ◽  
Optimal Growth ◽  
Water Infiltration ◽  
Evaporative Water ◽  
Factors Affecting ◽  
Geomyces Destructans ◽  
Thermal Requirements ◽  
White Nose Syndrome ◽  
Air Infiltration

The fungal pathogen Geomyces destructans, which causes white-nose syndrome in bats, thrives in the cold and moist conditions found in caves where bats hibernate. To aid managers and researchers address this disease, an updated and accessible review of cave hibernacula and cave microclimates is presented. To maximize energy savings and reduce evaporative water loss during winter, most temperate vespertilionid bats in North America select caves with temperatures between 2 and 10 °C, with 60%–100% relative humidity. Generally, the temperature in caves is similar to the mean annual surface temperature (MAST) of a region, which varies by latitude, altitude, and topography. However, MAST for most areas where caves are found in eastern North America is well above 10 °C. Thus, various factors cause cold-air infiltration that reduces temperatures of these caves during winter. These factors include depth of cave, topographic setting, airflow patterns, cave configuration, and water infiltration. Factors affecting humidity, condensation, and evaporation are also addressed. In areas where MAST is above or below the thermal requirements of Geomyces destructans, many caves used by bats as hibernacula may still provide favorable sites for optimal growth of this fungus.

Geomyces destructans sp. nov. associated with bat white-nose syndrome

Mycotaxon ◽  
2009 ◽  
Vol 108 (1) ◽  
pp. 147-154 ◽  
Author(s):  
A. Gargas ◽  
M.T. Trest ◽  
M. Christensen ◽  
T.J. Volk ◽  
D.S. Blehert
Keyword(s):  
Geomyces Destructans ◽  
White Nose Syndrome

scholarly journals Temperature-Dependent Growth of Geomyces destructans, the Fungus That Causes Bat White-Nose Syndrome

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46280 ◽  
Author(s):  
Michelle L. Verant ◽  
Justin G. Boyles ◽  
William Waldrep ◽  
Gudrun Wibbelt ◽  
David S. Blehert
Keyword(s):  
Temperature Dependent ◽  
Geomyces Destructans ◽  
White Nose Syndrome ◽  
Dependent Growth

Rapid Real-Time PCR Assay for Culture and Tissue Identification of Geomyces destructans: the Etiologic Agent of Bat Geomycosis (White Nose Syndrome)

2011 ◽  
Vol 172 (4) ◽  
pp. 247-256 ◽  
Author(s):  
Sudha Chaturvedi ◽  
Robert J. Rudd ◽  
April Davis ◽  
Tanya R. Victor ◽  
Xiaojiang Li ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Etiologic Agent ◽  
Pcr Assay ◽  
Geomyces Destructans ◽  
White Nose Syndrome ◽  
Tissue Identification

scholarly journals Mycobiome of the Bat White Nose Syndrome Affected Caves and Mines Reveals Diversity of Fungi and Local Adaptation by the Fungal Pathogen Pseudogymnoascus (Geomyces) destructans

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e108714 ◽  
Author(s):  
Tao Zhang ◽  
Tanya R. Victor ◽  
Sunanda S. Rajkumar ◽  
Xiaojiang Li ◽  
Joseph C. Okoniewski ◽  
...  
Keyword(s):  
Local Adaptation ◽  
Fungal Pathogen ◽  
Geomyces Destructans ◽  
White Nose Syndrome

scholarly journals Morphological and Molecular Characterizations of Psychrophilic Fungus Geomyces destructans from New York Bats with White Nose Syndrome (WNS)

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e10783 ◽  
Author(s):  
Vishnu Chaturvedi ◽  
Deborah J. Springer ◽  
Melissa J. Behr ◽  
Rama Ramani ◽  
Xiaojiang Li ◽  
...  
Keyword(s):  
New York ◽  
Geomyces Destructans ◽  
White Nose Syndrome ◽  
Psychrophilic Fungus
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