Examination of several Oklahoma bat hibernacula cave soils forPseudogymnoascus destructans,the causative agent of White-Nose Syndrome

2015 ◽  
Vol 60 (2-3) ◽  
pp. 213-217 ◽  
Author(s):  
James P. Creecy ◽  
William Caire ◽  
Kylie A. Gilcrest
Keyword(s):  
Causative Agent ◽  
White Nose Syndrome

scholarly journals Bacteria Isolated from Bats Inhibit the Growth of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0121329 ◽  
Author(s):  
Joseph R. Hoyt ◽  
Tina L. Cheng ◽  
Kate E. Langwig ◽  
Mallory M. Hee ◽  
Winifred F. Frick ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome

scholarly journals Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome

2015 ◽  
Vol 112 (24) ◽  
pp. 7478-7483 ◽  
Author(s):  
Anthony J. O’Donoghue ◽  
Giselle M. Knudsen ◽  
Chapman Beekman ◽  
Jenna A. Perry ◽  
Alexander D. Johnson ◽  
...  
Keyword(s):  
Causative Agent ◽  
Genetic Manipulation ◽  
Hydrolytic Enzymes ◽  
Collagen Degradation ◽  
Mammalian Host ◽  
Peptidase Activity ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome ◽  
Proteolytic Activities ◽  
The Impact

Pseudogymnoascus destructans is the causative agent of white-nose syndrome, a disease that has caused the deaths of millions of bats in North America. This psychrophilic fungus proliferates at low temperatures and targets hibernating bats, resulting in their premature arousal from stupor with catastrophic consequences. Despite the impact of white-nose syndrome, little is known about the fungus itself or how it infects its mammalian host. P. destructans is not amenable to genetic manipulation, and therefore understanding the proteins involved in infection requires alternative approaches. Here, we identify hydrolytic enzymes secreted by P. destructans, and use a novel and unbiased substrate profiling technique to define active peptidases. These experiments revealed that endopeptidases are the major proteolytic activities secreted by P. destructans, and that collagen, the major structural protein in mammals, is actively degraded by the secretome. A serine endopeptidase, hereby-named Destructin-1, was subsequently identified, and a recombinant form overexpressed and purified. Biochemical analysis of Destructin-1 showed that it mediated collagen degradation, and a potent inhibitor of peptidase activity was identified. Treatment of P. destructans-conditioned media with this antagonist blocked collagen degradation and facilitated the detection of additional secreted proteolytic activities, including aminopeptidases and carboxypeptidases. These results provide molecular insights into the secretome of P. destructans, and identify serine endopeptidases that have the clear potential to facilitate tissue invasion and pathogenesis in the mammalian host.

Examination of bats in western Oklahoma for antibodies againstPseudogymnoascus destructans, the causative agent of White-Nose Syndrome

2015 ◽  
Vol 60 (2-3) ◽  
pp. 145-150 ◽  
Author(s):  
Robert E. Brennan ◽  
William Caire ◽  
Nicholas Pugh ◽  
Susan Chapman ◽  
Alison H. Robbins ◽  
...  
Keyword(s):  
Causative Agent ◽  
White Nose Syndrome

scholarly journals Evaluation of Strategies for the Decontamination of Equipment for Geomyces destructans, the Causative Agent of the White-Nose Syndrome (WNS)

2013 ◽  
pp. 1-10 ◽  
Author(s):  
Virginia Shelley ◽  
Samantha Kaiser ◽  
Elizabeth Shelley ◽  
Tim Williams ◽  
Marcelo Kramer ◽  
...  
Keyword(s):  
Causative Agent ◽  
Geomyces Destructans ◽  
White Nose Syndrome

Long-Term Persistence of Pseudogymnoascus destructans, the Causative Agent of White-Nose Syndrome, in the Absence of Bats

EcoHealth ◽  
2014 ◽  
Vol 12 (2) ◽  
pp. 330-333 ◽  
Author(s):  
Joseph R. Hoyt ◽  
Kate E. Langwig ◽  
Joseph Okoniewski ◽  
Winifred F. Frick ◽  
Ward B. Stone ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pseudogymnoascus Destructans ◽  
White Nose Syndrome
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