Tissue cultivation of tiller buds of Epimedium wushanense

2012 ◽  
Keyword(s):  
Tissue Cultivation

Application of open porous poly(D,L-lactide-co-glycolide) microspheres and the strategy of hydrophobic seeding in hepatic tissue cultivation

2013 ◽  
Vol 101 (10) ◽  
pp. 2862-2869 ◽  
Author(s):  
Ming-Ju Chou ◽  
Chin-Hsiung Hsieh ◽  
Peng-Lin Yeh ◽  
Po-Cheng Chen ◽  
Ching-Hua Wang ◽  
...  
Keyword(s):  
Hepatic Tissue ◽  
Tissue Cultivation ◽  
Porous Poly

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: II. INVESTIGATION OF VARIOUS ENZYME SYSTEMS IN HOMOGENATES OF KIDNEY TISSUE CULTURES OF NORMAL RHESUS MONKEYS

1956 ◽  
Vol 34 (3) ◽  
pp. 600-618 ◽  
Author(s):  
E. Kovacs
Keyword(s):  
Acid Metabolism ◽  
Kidney Tissue ◽  
Nucleic Acid Metabolism ◽  
Enzyme Assays ◽  
Tissue Cultures ◽  
Functional Tests ◽  
Alkaline Phosphatases ◽  
Enzyme Systems ◽  
Tissue Cultivation ◽  
Considerable Loss

Pools of normal tissue cultures were examined for enzymes associated with nucleic acid metabolism. Ribonucleases and desoxyribonucleases, 5-nucleotidases, simple nucleotidases, acid and alkaline phosphatases were studied, and certain others occasionally demonstrated. Characteristic behavior of these enzyme systems during the cultivation procedures, during growth, and during degeneration was described. Quantitative data indicate the presence of significant amounts of enzymes in the supernatant fluid. This accounts for the considerable loss in these specialized constituents during fluid changes. The bearing of these findings on the physiology and pathology of cultivated cells was discussed, as a working hypothesis, with special emphasis on poliomyelitis infection. The use of enzyme assays, as functional tests supplementing morphological methods in tissue cultivation, was recommended.

The Application of Soybean Inhibitor in Tissue Cultivation

Science ◽  
1949 ◽  
Vol 109 (2842) ◽  
pp. 611-612 ◽  
Author(s):  
A. Fischer
Keyword(s):  
Tissue Cultivation

Tissue cultivation of rose-colored periwinkle. Biosynthesis of indole alkaloids

1978 ◽  
Vol 12 (8) ◽  
pp. 1048-1050
Author(s):  
T. I. Andreeva ◽  
L. N. Bereznegovskaya ◽  
A. V. Smorodin
Keyword(s):  
Indole Alkaloids ◽  
Tissue Cultivation

COMPARATIVE BIOCHEMICAL STUDIES ON NORMAL AND ON POLIOMYELITIS INFECTED TISSUE CULTURES: III. ENZYME ASSAYS ON HOMOGENATES OF SURVIVING NORMAL RHESUS KIDNEY. EFFECT OF SYNTHETIC NUTRIENT MIXTURES

1956 ◽  
Vol 34 (1) ◽  
pp. 619-636 ◽  
Author(s):  
Ernest Kovacs
Keyword(s):  
Synthetic Medium ◽  
Enzyme Assays ◽  
Fresh Tissue ◽  
Normal Tissues ◽  
Salt Mixtures ◽  
Tissue Homogenates ◽  
Synthetic Media ◽  
Tissue Cultivation ◽  
Enzyme Alkaline Phosphatase

Investigation of enzyme systems in normal tissues is the main subject of this communication. The activity of phosphatases, nucleotidases, and nucleases was explored in "brei" prepared with water, physiological salt mixtures, and synthetic media used for tissue cultivation, to compare the influence of these diluents. The depression of acid phosphatase by medium 597, noted previously in cells growing in vitro, was confirmed in fresh tissue and definitely proved on purified enzyme. Alkaline phosphatase was activated by the same synthetic medium. Differences in kinetics were observed when the effect of medium 597 was examined on 5-nucleotidase concentrate of Russel's viper venom and on pentanucleotidase of fresh kidney. Crystalline RNA-se was inhibited, in a manner similar to the nucleases of kidney extracts. Both phosphomonoesterases were enhanced by the less complex nutrient 697. The deterioration of DNA-se in medium 199 upon storage was striking. Significant observations were gathered on enzymes of concentrated and very diluted (1: 1600) tissue homogenates, on extracts, and on isolated enzymes of various grades of purity.

scholarly journals Low frequency ultrasound as an enhancer for the germination process of Stizolobium pruriens

2021 ◽  
Vol 12 (5) ◽  
pp. 765-776
Author(s):  
Lucas Menezes Felizardo ◽  
Beatriz Garcia Lopes ◽  
Glaucia Amorim Faria ◽  
Adrielle Rodrigues Prates ◽  
Gabriela Lozano Oliveiro ◽  
...  
Keyword(s):  
Crop Rotation ◽  
Plant Tissue ◽  
Low Frequency ◽  
Ultrasound Exposure ◽  
Germination Process ◽  
Germination Speed ◽  
Low Frequency Ultrasound ◽  
Germination Vigor ◽  
Tissue Cultivation ◽  
Frequency Ultrasound

Mucuna (Stizolobium pruriens) is widely used in agriculture as a green allowance and in crop rotation, due to its ability to fix nitrogen and recover degraded areas; without embargo, there is a slow and uneven germination. This study used some classical methods, together with the use of low-frequency ultrasound to accelerate and homogenize the germination and emergence of the seeds. The experiment was carried out at the Plant Tissue Cultivation Laboratory of the Ilha Solteira Campus, São Paulo, Brazil. The design used was a completely randomized one, with five replications, in a 3x6 factorial scheme, the factors being: three pre-treatments for latency break: mechanical scarification, thermal scarification, and without scarification with six levels of ultrasound exposure: 0, 1, 2, 3, 5, 8 min, totaling 18 treatments. For eight days the germination and the initial stages of the seedlings were controlled. The method without scarification subjected to 4.5 min of ultrasound can become an excellent alternative, since it presented greater germination vigor, while 3.14 min of exposure to ultrasound were enough to improve the emergence speed, regardless of the method used in the preparation of seeds. In conclusion, only with the use of low-frequency ultrasound, it is possible to improve both the germination speed index and the germination vigor, without the need for additional treatments.

A device for nerve tissue cultivation

1975 ◽  
Vol 80 (2) ◽  
pp. 991-992 ◽  
Author(s):  
I. V. Viktorov ◽  
E. M. Kenarskaya
Keyword(s):  
Nerve Tissue ◽  
Tissue Cultivation
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