scholarly journals Characterization of Exo 1, 4-<i>β</i> glucanase produced from <i>Tricoderma Viridi</i> MBL through solid-state bio-processing of orange peel waste

2012 ◽  
Vol 03 (05) ◽  
pp. 580-584 ◽  
Author(s):  
Muhammad Irshad ◽  
Zahid Anwar ◽  
Amber Afroz
2015 ◽  
Vol 101 ◽  
pp. 1-8 ◽  
Author(s):  
Fani Th Mantzouridou ◽  
Adamantini Paraskevopoulou ◽  
Sofia Lalou

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Danielle Biscaro Pedrolli ◽  
Eleonora Cano Carmona

A pectin lyase, named PLIII, was purified to homogeneity from the culture filtrate of Aspergillus giganteus grown in submerged culture containing orange peel waste as carbon source. PLIII was able to digest apple pectin and citrus pectins with different degrees of methyl esterification. Interestingly, the PLIII activity was stimulated in the presence of some divalent cations including Pb2+ and was not significantly affected by Hg2+. Like other pectin lyases, PLIII is stimulated by but is not dependent on Ca2+. The main soluble product released during the degradation of pectic substances promoted by the PLIII is compatible with an unsaturated monogalacturonate. PLIII is a unique enzyme able to release unsaturated monogalacturonate as the only soluble product during the degradation of pectic substances; therefore, PLIII was classified as an exo-pectin lyase. To our knowledge, this is the first characterization of an exo-pectin lyase. The PLIII described in this work is potentially useful for ethanol production from pectin-rich biomass, besides other common applications for alkaline pectinases like preparation of textile fibers, coffee and tea fermentation, vegetable oil extraction, and the treatment of pulp in papermaking.


2013 ◽  
Vol 04 (10) ◽  
pp. 941-944 ◽  
Author(s):  
Muhammad Irshad ◽  
Zahid Anwar ◽  
Muhammad Ramzan ◽  
Zahed Mahmood ◽  
Haq Nawaz

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1348
Author(s):  
José R. Ayala ◽  
Gisela Montero ◽  
Marcos A. Coronado ◽  
Conrado García ◽  
Mario A. Curiel-Alvarez ◽  
...  

Annually, millions of tons of foods are generated with the purpose to feed the growing world population. One particular eatable is orange, the production of which in 2018 was 75.54 Mt. One way to valorize the orange residue is to produce bioethanol by fermenting the reducing sugars generated from orange peel. Hence, the objective of the present work was to determine the experimental conditions to obtain the maximum yield of reducing sugars from orange peel using a diluted acid hydrolysis process. A proximate and chemical analysis of the orange peel were conducted. For the hydrolysis, two factorial designs were prepared to measure the glucose and fructose concentration with the 3,5-DNS acid method and UV-Visible spectroscopy. The factors were acid concentration, temperature and hydrolysis time. After the hydrolysis, the orange peel samples were subjected to an elemental SEM-EDS analysis. The results for the orange peel were 73.530% of moisture, 99.261% of volatiles, 0.052% of ash, 0.687% of fixed carbon, 19.801% of lignin, 69.096% of cellulose and 9.015% of hemicellulose. The highest concentration of glucose and fructose were 24.585 and 9.709 g/L, respectively. The results highlight that sugar production is increased by decreasing the acid concentration.


2014 ◽  
Vol 79 (6) ◽  
pp. N1218-N1224 ◽  
Author(s):  
Akihiro Hideno ◽  
Kentaro Abe ◽  
Hiroyuki Yano

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1869
Author(s):  
A K M Mashud Alam ◽  
Donovan Jenks ◽  
George A. Kraus ◽  
Chunhui Xiang

Organophosphate (OP) compounds, a family of highly hazardous chemical compounds included in nerve agents and pesticides, have been linked to more than 250,000 annual deaths connected to various chronic diseases. However, a solid-state sensing system that is able to be integrated into a clothing system is rare in the literature. This study aims to develop a nanofiber-based solid-state polymeric material as a soft sensor to detect OP compounds present in the environment. Esters of polydiacetylene were synthesized and incorporated into a cellulose acetate nanocomposite fibrous assembly developed with an electrospinning technique, which was then hydrolyzed to generate more hydroxyl groups for OP binding. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), Instron® tensile tester, contact angle analyzer, and UV–Vis spectroscopy were employed for characterizations. Upon hydrolysis, polydiacetylene esters in the cellulosic fiber matrix were found unaffected by hydrolysis treatment, which made the composites suitable for OP sensing. Furthermore, the nanofibrous (NF) composites exhibited tensile properties suitable to be used as a textile material. Finally, the NF composites exhibited colorimetric sensing of OP, which is visible to the naked eye. This research is a landmark study toward the development of OP sensing in a protective clothing system.


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