scholarly journals Temporal differences in DNA replication during the S phase using single fiber analysis of normal human fibroblasts and glioblastoma T98G cells

Cell Cycle ◽  
2009 ◽  
Vol 8 (19) ◽  
pp. 3133-3148 ◽  
Author(s):  
Rebecca A. Frum ◽  
Zakaria S. Khondker ◽  
David G. Kaufman
Keyword(s):  
Dna Replication ◽  
Human Fibroblasts ◽  
S Phase ◽  
Single Fiber ◽  
Temporal Differences ◽  
Fiber Analysis ◽  
Normal Human ◽  
Single Fiber Analysis

scholarly journals Separation of intra-S checkpoint protein contributions to DNA replication fork protection and genomic stability in normal human fibroblasts

Cell Cycle ◽  
2013 ◽  
Vol 12 (2) ◽  
pp. 332-345 ◽  
Author(s):  
Stephanie L. Smith-Roe ◽  
Shivani S. Patel ◽  
Yingchun Zhou ◽  
Dennis A. Simpson ◽  
Shangbang Rao ◽  
...  
Keyword(s):  
Dna Replication ◽  
Replication Fork ◽  
Human Fibroblasts ◽  
Genomic Stability ◽  
Checkpoint Protein ◽  
Normal Human

Identification of Chromosomal Bands Replicating Early in the S Phase of Normal Human Fibroblasts

1998 ◽  
Vol 245 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Stephanie M. Cohen ◽  
Erica R. Cobb ◽  
Marila Cordeiro-Stone ◽  
David G. Kaufman
Keyword(s):  
Human Fibroblasts ◽  
S Phase ◽  
Normal Human ◽  
Chromosomal Bands

Single-fiber analysis of mitochondrial A3243G mutation in four different phenotypes

2000 ◽  
Vol 99 (2) ◽  
pp. 186-190 ◽  
Author(s):  
Yasutoshi Koga ◽  
Atsuko Koga ◽  
Rikako Iwanaga ◽  
Yukihiro Akita ◽  
Junko Tubone ◽  
...  
Keyword(s):  
Single Fiber ◽  
Fiber Analysis ◽  
A3243g Mutation ◽  
Single Fiber Analysis

Construction of a cosmid library of DNA replicated early in the S phase of normal human fibroblasts

2000 ◽  
Vol 78 (3) ◽  
pp. 509-517 ◽  
Author(s):  
Bruna P. Brylawski ◽  
Stephanie M. Cohen ◽  
Jonathan L. Longmire ◽  
Norman A. Doggett ◽  
Marila Cordeiro-Stone ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
S Phase ◽  
Cosmid Library ◽  
Normal Human

Single Fiber Analysis by Internal Reflection Infrared Microspectroscopy

2001 ◽  
Vol 46 (6) ◽  
pp. 15151J ◽  
Author(s):  
Liling Cho ◽  
John A. Reffner ◽  
Barbara M. Gatewood ◽  
David L. Wetzel
Keyword(s):  
Internal Reflection ◽  
Single Fiber ◽  
Infrared Microspectroscopy ◽  
Fiber Analysis ◽  
Single Fiber Analysis

scholarly journals Senescent human diploid fibroblasts are able to support DNA synthesis and to express markers associated with proliferation

1990 ◽  
Vol 97 (3) ◽  
pp. 473-478
Author(s):  
I.R. Kill ◽  
S. Shall
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Human Fibroblasts ◽  
S Phase ◽  
Growth Media ◽  
Transient Phase ◽  
Human Diploid Fibroblasts ◽  
Reproductive Life ◽  
Reproductive Life Span ◽  
Normal Human

The characteristic limited reproductive life-span of normal human fibroblasts in culture is due to a steadily decreasing fraction of cells able to proliferate in the standard rich growth media. We have observed that restricting the growth factor supply to old cells for variable lengths of time in culture increases the fraction of cells that can enter S-phase; although these cells do not go on to divide. Thus, it seems that there is a transient phase between the proliferating state and the irreversibly post-mitotic, senescent state. Perhaps a ‘quiescent-G0’ state, which can be maintained in the presence of growth factors, is a stage on the pathway to mortalization and senescence.

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