Identification of Chromosomal Bands Replicating Early in the S Phase of Normal Human Fibroblasts

1998 ◽  
Vol 245 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Stephanie M. Cohen ◽  
Erica R. Cobb ◽  
Marila Cordeiro-Stone ◽  
David G. Kaufman
Keyword(s):  
Human Fibroblasts ◽  
S Phase ◽  
Normal Human ◽  
Chromosomal Bands

Construction of a cosmid library of DNA replicated early in the S phase of normal human fibroblasts

2000 ◽  
Vol 78 (3) ◽  
pp. 509-517 ◽  
Author(s):  
Bruna P. Brylawski ◽  
Stephanie M. Cohen ◽  
Jonathan L. Longmire ◽  
Norman A. Doggett ◽  
Marila Cordeiro-Stone ◽  
...  
Keyword(s):  
Human Fibroblasts ◽  
S Phase ◽  
Cosmid Library ◽  
Normal Human

scholarly journals Senescent human diploid fibroblasts are able to support DNA synthesis and to express markers associated with proliferation

1990 ◽  
Vol 97 (3) ◽  
pp. 473-478
Author(s):  
I.R. Kill ◽  
S. Shall
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Human Fibroblasts ◽  
S Phase ◽  
Growth Media ◽  
Transient Phase ◽  
Human Diploid Fibroblasts ◽  
Reproductive Life ◽  
Reproductive Life Span ◽  
Normal Human

The characteristic limited reproductive life-span of normal human fibroblasts in culture is due to a steadily decreasing fraction of cells able to proliferate in the standard rich growth media. We have observed that restricting the growth factor supply to old cells for variable lengths of time in culture increases the fraction of cells that can enter S-phase; although these cells do not go on to divide. Thus, it seems that there is a transient phase between the proliferating state and the irreversibly post-mitotic, senescent state. Perhaps a ‘quiescent-G0’ state, which can be maintained in the presence of growth factors, is a stage on the pathway to mortalization and senescence.

scholarly journals Precursor-Boosted Production of Metabolites in Nasturtium officinale Microshoots Grown in Plantform Bioreactors, and Antioxidant and Antimicrobial Activities of Biomass Extracts

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4660
Author(s):  
Marta Klimek-Szczykutowicz ◽  
Michał Dziurka ◽  
Ivica Blažević ◽  
Azra Đulović ◽  
Małgorzata Miazga-Karska ◽  
...  
Keyword(s):  
Photosynthetic Pigments ◽  
Human Fibroblasts ◽  
Antimicrobial Activities ◽  
Precursor Feeding ◽  
Nasturtium Officinale ◽  
Qualitative And Quantitative ◽  
Normal Human ◽  
Inhibition Zones ◽  
Ferulic Acids ◽  
Antioxidant And Antimicrobial Activities

The study demonstrated the effects of precursor feeding on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in Nasturtium officinale microshoot cultures grown in Plantform bioreactors. It also evaluated the antioxidant and antimicrobial activities of extracts. L-phenylalanine (Phe) and L-tryptophan (Trp) as precursors were tested at 0.05, 0.1, 0.5, 1.0, and 3.0 mM. They were added at the beginning (day 0) or on day 10 of the culture. Microshoots were harvested after 20 days. Microshoots treated with 3.0 mM Phe (day 0) had the highest total GSL content (269.20 mg/100 g DW). The qualitative and quantitative profiles of the GSLs (UHPLC-DAD-MS/MS) were influenced by precursor feeding. Phe at 3.0 mM stimulated the best production of 4-methoxyglucobrassicin (149.99 mg/100 g DW) and gluconasturtiin (36.17 mg/100 g DW). Total flavonoids increased to a maximum of 1364.38 mg/100 g DW with 3.0 mM Phe (day 0), and polyphenols to a maximum of 1062.76 mg/100 g DW with 3.0 mM Trp (day 0). The precursors also increased the amounts of p-coumaric and ferulic acids, and rutoside, and generally increased the production of active photosynthetic pigments. Antioxidant potential increased the most with 0.1 mM Phe (day 0) (CUPRAC, FRAP), and with 0.5 mM Trp (day 10) (DPPH). The extracts of microshoots treated with 3.0 mM Phe (day 0) showed the most promising bacteriostatic activity against microaerobic Gram-positive acne strains (MIC 250–500 µg/mL, 20–21 mm inhibition zones). No extract was cytotoxic to normal human fibroblasts over the tested concentration range (up to 250 μg/mL).

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