Single Fiber Analysis by Internal Reflection Infrared Microspectroscopy

2001 ◽  
Vol 46 (6) ◽  
pp. 15151J ◽  
Author(s):  
Liling Cho ◽  
John A. Reffner ◽  
Barbara M. Gatewood ◽  
David L. Wetzel
Keyword(s):  
Internal Reflection ◽  
Single Fiber ◽  
Infrared Microspectroscopy ◽  
Fiber Analysis ◽  
Single Fiber Analysis

Single-fiber analysis of mitochondrial A3243G mutation in four different phenotypes

2000 ◽  
Vol 99 (2) ◽  
pp. 186-190 ◽  
Author(s):  
Yasutoshi Koga ◽  
Atsuko Koga ◽  
Rikako Iwanaga ◽  
Yukihiro Akita ◽  
Junko Tubone ◽  
...  
Keyword(s):  
Single Fiber ◽  
Fiber Analysis ◽  
A3243g Mutation ◽  
Single Fiber Analysis

Attenuated Total Internal Reflection Infrared Microspectroscopy For The Study Of Trace Contaminants In Aqueous Solutions.

1999 ◽  
Vol 5 (S2) ◽  
pp. 66-67
Author(s):  
Andre’ J. Sommer ◽  
Mark Hardgrove
Keyword(s):  
Infrared Spectroscopy ◽  
Total Internal Reflection ◽  
Molecular Spectroscopy ◽  
Internal Reflection ◽  
Small Samples ◽  
Attenuated Total Internal Reflection ◽  
Infrared Microspectroscopy ◽  
Transmission Measurements ◽  
Major Emphasis

Over the past several years many developments have taken place in the field of molecular spectroscopy. For Raman spectroscopy many of the improvements have arisen from technological innovations that include diode-based lasers, holographic notch filters and charged coupled detectors. In contrast, a majority of the developments in infrared spectroscopy have been in the area of new sampling accessories. A major emphasis has been placed on attenuated total internal reflection (ATR) accessories. The devices are allowing infrared spectroscopy to be employed in process control environments and quality control laboratories where the method is not only robust but has the advantages of limited sample preparation and/or in situ analysis.In the realm of microspectroscopy, ATR accessories have the added advantages of providing better spatial resolution, equal to or higher S/N for equivalent sample size compared to transmission measurements and most importantly the ability to collect spectra of small samples without the adverse effect of diffraction. One accessory which was developed several years ago is known as the Split-Pea.

A Comparison of Immersion Infrared Microspectroscopy to Attenuated Total Internal Reflection Microspectroscopy

2004 ◽  
Vol 10 (S02) ◽  
pp. 1298-1299 ◽  
Author(s):  
L. E. Lavalle ◽  
A. J. Sommer ◽  
G. M. Story ◽  
A. E. Dowery ◽  
C. Marcott
Keyword(s):  
Total Internal Reflection ◽  
Internal Reflection ◽  
Attenuated Total Internal Reflection ◽  
Infrared Microspectroscopy

Extended abstract of a paper presented at Microscopy and Microanalysis 2004 in Savannah, Georgia, USA, August 1–5, 2004.

Use of Molecular Microspectroscopy to Characterize Pigment-Loaded Polypropylene Single Fibers

1994 ◽  
Vol 48 (11) ◽  
pp. 1387-1393 ◽  
Author(s):  
S. P. Bouffard ◽  
A. J. Sommer ◽  
J. E. Katon ◽  
S. Godber
Keyword(s):  
Correlation Coefficient ◽  
Polypropylene Fibers ◽  
Effective Technique ◽  
Pigment Identification ◽  
Infrared Microspectroscopy ◽  
Positive Identification ◽  
Fiber Analysis ◽  
Heating Effects ◽  
Relative Standard ◽  
Concentration Levels

Molecular microspectroscopy was used to characterize different pigments loaded into polypropylene fibers. Single-fiber analysis by infrared, visible, and Raman microspectroscopies proved to be quite complementary for identification and quantitation of these materials. Infrared microspectroscopy was effective not only for identification of pigments loaded into the fibers, but also for quantitation of concentration levels at 1% (w/w) and greater. Visible microspectroscopy was effective for quantitation of pigment levels between the range of 0.1 and 1% (w/w). However, it may not be successful for positive identification of most pigments. Raman microspectroscopy was an effective technique for pigment identification and quantitation over all examined concentration levels provided that the sample showed no signs of fluorescence or heating effects. Quantitation for all techniques gave correlation coefficient values of 0.99+ and relative standard deviations typically ranging from 5 to 20%.

Attenuated Total Internal Reflection Infrared Mapping Microspectroscopy of Soft Materials

2000 ◽  
Vol 54 (2) ◽  
pp. 324-330 ◽  
Author(s):  
Lori L. Lewis ◽  
André J. Sommer
Keyword(s):  
Spatial Resolution ◽  
Total Internal Reflection ◽  
Soft Materials ◽  
Diffraction Limit ◽  
Internal Reflection ◽  
Attenuated Total Internal Reflection ◽  
Infrared Microspectroscopy ◽  
Soft Samples ◽  
Polymer Laminates

Attenuated total internal reflection (ATR) infrared mapping microspectroscopy of soft samples is reviewed and investigated by using cartridge-based germanium hemispheres. The study demonstrates the use of these devices for obtaining line scans or maps of soft pliable surfaces over an area of approximately 100 × 100 micrometers. An experimental determination of the spatial resolution by using a cross-sectioned polymer film showed a twofold improvement over transmission infrared microspectroscopy for sample sizes at the diffraction limit. Optical details of the devices are discussed in the context of ATR measurements in addition to their application for the study of polymer laminates often encountered in industry and forensics.

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