A facile and real-time spectroscopic method for biofluid analysis in point-of-care diagnostics

Bioanalysis ◽  
2013 ◽  
Vol 5 (15) ◽  
pp. 1853-1861 ◽  
Author(s):  
Rajesh Kumar ◽  
Gajendra P Singh ◽  
Ishan Barman ◽  
Narahara Chari Dingari ◽  
Ghulam Nabi
Keyword(s):  
Real Time ◽  
Point Of Care ◽  
Spectroscopic Method ◽  
Point Of Care Diagnostics

scholarly journals Advances in Miniaturized Instruments for Genomics

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Cihun-Siyong Alex Gong ◽  
Kin Fong Lei
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Potential Application ◽  
Point Of Care ◽  
Microfluidic Systems ◽  
Promising Technique ◽  
Point Of Care Diagnostics ◽  
Recent Developments ◽  
Solid Foundation

In recent years, a lot of demonstrations of the miniaturized instruments were reported for genomic applications. They provided the advantages of miniaturization, automation, sensitivity, and specificity for the development of point-of-care diagnostics. The aim of this paper is to report on recent developments on miniaturized instruments for genomic applications. Based on the mature development of microfabrication, microfluidic systems have been demonstrated for various genomic detections. Since one of the objectives of miniaturized instruments is for the development of point-of-care device, impedimetric detection is found to be a promising technique for this purpose. An in-depth discussion of the impedimetric circuits and systems will be included to provide total consideration of the miniaturized instruments and their potential application towards real-time portable imaging in the “-omics” era. The current excellent demonstrations suggest a solid foundation for the development of practical and widespread point-of-care genomic diagnostic devices.

scholarly journals Competitive Volumetric Bar-Chart Chip with Real-Time Internal Control for Point-of-Care Diagnostics

2015 ◽  
Vol 87 (7) ◽  
pp. 3771-3777 ◽  
Author(s):  
Ying Li ◽  
Jie Xuan ◽  
Tom Xia ◽  
Xin Han ◽  
Yujun Song ◽  
...  
Keyword(s):  
Real Time ◽  
Internal Control ◽  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals Erratum to: Real-time electrical detection of epidermal skin MoS2 biosensor for point-of-care diagnostics

Nano Research ◽  
2017 ◽  
Vol 10 (3) ◽  
pp. 1105-1105
Author(s):  
Geonwook Yoo ◽  
Heekyeong Park ◽  
Minjung Kim ◽  
Won Geun Song ◽  
Seokhwan Jeong ◽  
...  
Keyword(s):  
Real Time ◽  
Point Of Care ◽  
Electrical Detection ◽  
Point Of Care Diagnostics

Reusable Surface Molecular Imprint Biosensors Aided by Naturally Occurring Surface Roughness Indices for Point-of-Care Diagnostics

MRS Advances ◽  
2019 ◽  
Vol 4 (22) ◽  
pp. 1299-1308 ◽  
Author(s):  
Yehoshua Auerbach ◽  
Rebecca Isseroff ◽  
Nicholas Clayton ◽  
Miguel Hulyalkar ◽  
Andrew Todt ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Imprinting ◽  
Point Of Care ◽  
High Sensitivity ◽  
Cost Effective ◽  
Stem Cell Differentiation ◽  
Diverse Range ◽  
Point Of Care Diagnostics ◽  
Molecular Imprint ◽  
Detection Of Biomarkers

ABSTRACTWe have shown that molecular imprinting (MI) technology can be used to produce sensitive, robust, cost-effective biosensing systems with a real-time electrochemical readout that can be utilized for point of care diagnostics. Real time detection of biomarkers is essential when rapid, critical decisions need to be made, such as in situations where public health is threatened. Our biosensor has high sensitivity compared to standard methods like ELISA, and results are obtained within minutes, using inexpensive, accessible potentiometric readout technology. These biosensors utilize surface molecular imprinting of a self-assembling monolayer of hydroxy-terminated alkanethiol chains which form a crystalline ‘lock-and-key’ structure around a target analyte, allowing the sensors to detect and differentiate between bio-macromolecules of similar size and shape with high selectivity and sensitivity. The sensors are extremely versatile and able to detect a diverse range of molecules of varied chemical composition and structure. To fully exploit the sensors’ advantages, especially in remote, economically disadvantaged areas, it is important to quantify their durability and reusability. Biosensor chips were created to test the viability of hemoglobin detection and to evaluate the potential for sensor reusability when washed after detection testing. The successful readsorption of hemoglobin even after washing, accompanied by cyclic voltammetry data indicating the preservation of the SAM, indicate that these biosensors are reusable, significantly augmenting the device’s value. Potential applications include the analysis of complex chemical and biological processes such as stem cell differentiation and on-the-spot detection of diseases such as Zika.

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

Real-time electrical detection of epidermal skin MoS2 biosensor for point-of-care diagnostics

Nano Research ◽  
2016 ◽  
Vol 10 (3) ◽  
pp. 767-775 ◽  
Author(s):  
Geonwook Yoo ◽  
Heekyeong Park ◽  
Minjung Kim ◽  
Won Geun Song ◽  
Seokhwan Jeong ◽  
...  
Keyword(s):  
Real Time ◽  
Point Of Care ◽  
Electrical Detection ◽  
Point Of Care Diagnostics

scholarly journals Combining Point-of-Care Diagnostics and Internet of Medical Things (IoMT) to Combat the COVID-19 Pandemic

Diagnostics ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 224 ◽  
Author(s):  
Ting Yang ◽  
Mattia Gentile ◽  
Ching-Fen Shen ◽  
Chao-Min Cheng
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Point Of Care ◽  
Pcr Assay ◽  
Current Standard ◽  
Standard Testing ◽  
Testing Method ◽  
Point Of Care Diagnostics ◽  
Internet Of Medical Things

The current standard testing method for screening coronavirus disease 2019 (COVID-19) is through reverse real-time PCR assay (rRT-PCR), a common molecular-based assay that requires an average of four to six hours to provide results [...]

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