scholarly journals Advances in Miniaturized Instruments for Genomics

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Cihun-Siyong Alex Gong ◽  
Kin Fong Lei
Keyword(s):  
Real Time ◽  
Sensitivity And Specificity ◽  
Potential Application ◽  
Point Of Care ◽  
Microfluidic Systems ◽  
Promising Technique ◽  
Point Of Care Diagnostics ◽  
Recent Developments ◽  
Solid Foundation

In recent years, a lot of demonstrations of the miniaturized instruments were reported for genomic applications. They provided the advantages of miniaturization, automation, sensitivity, and specificity for the development of point-of-care diagnostics. The aim of this paper is to report on recent developments on miniaturized instruments for genomic applications. Based on the mature development of microfabrication, microfluidic systems have been demonstrated for various genomic detections. Since one of the objectives of miniaturized instruments is for the development of point-of-care device, impedimetric detection is found to be a promising technique for this purpose. An in-depth discussion of the impedimetric circuits and systems will be included to provide total consideration of the miniaturized instruments and their potential application towards real-time portable imaging in the “-omics” era. The current excellent demonstrations suggest a solid foundation for the development of practical and widespread point-of-care genomic diagnostic devices.

Nanomechanics of superbugs and superdrugs: new frontiers in nanomedicine

2012 ◽  
Vol 40 (4) ◽  
pp. 603-608 ◽  
Author(s):  
Rachel A. McKendry
Keyword(s):  
Cell Wall ◽  
Drug Targets ◽  
Point Of Care ◽  
Cytoskeletal Proteins ◽  
Short Article ◽  
Point Of Care Diagnostics ◽  
New Antibiotics ◽  
Recent Developments ◽  
Force Resolution ◽  
New Generation

The alarming rise in drug-resistant hospital ‘superbugs’ and the associated increase in fatalities is driving the development of technologies to search for new antibiotics and improve disease diagnostics. One of the most successful drug targets is the bacterial cell wall, an evolutionary feature of virtually all prokaryotes and vital for their survival by providing mechanical strength. The recent discovery of bacterial cytoskeletal proteins analogous to the key force-bearing machinery in eukaryotes also provides new opportunities for drug discovery, but little is known about their mechanical role in bacteria. In the present short article, I review recent developments in the field of nanotechnology to investigate the mechanical mechanisms of action of potent antibiotics on cell wall and cytoskeletal targets with unprecedented spatial, temporal and force resolution and the development of a new generation of nanomechanical devices to detect pathogens for point-of-care diagnostics.

scholarly journals Point-of-Care Diagnostics: Recent Developments in a Connected Age

2016 ◽  
Vol 89 (1) ◽  
pp. 102-123 ◽  
Author(s):  
Samiksha Nayak ◽  
Nicole R. Blumenfeld ◽  
Tassaneewan Laksanasopin ◽  
Samuel K. Sia
Keyword(s):  
Point Of Care ◽  
Point Of Care Diagnostics ◽  
Recent Developments

Point-of-care diagnostics: recent developments in a pandemic age

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Harshit Harpaldas ◽  
Siddarth Arumugam ◽  
Chelsey Campillo Rodriguez ◽  
Bhoomika Ajay Kumar ◽  
Vivian Shi ◽  
...  
Keyword(s):  
Point Of Care ◽  
Digital Health ◽  
Point Of Care Diagnostics ◽  
The Future ◽  
Recent Developments

This article reviews the rapid and unprecedented development, scaleup and deployment of POC devices in response to the COVID-19 pandemic and its implications for the future of diagnostics and digital health.

scholarly journals Competitive Volumetric Bar-Chart Chip with Real-Time Internal Control for Point-of-Care Diagnostics

2015 ◽  
Vol 87 (7) ◽  
pp. 3771-3777 ◽  
Author(s):  
Ying Li ◽  
Jie Xuan ◽  
Tom Xia ◽  
Xin Han ◽  
Yujun Song ◽  
...  
Keyword(s):  
Real Time ◽  
Internal Control ◽  
Point Of Care ◽  
Point Of Care Diagnostics

scholarly journals Erratum to: Real-time electrical detection of epidermal skin MoS2 biosensor for point-of-care diagnostics

Nano Research ◽  
2017 ◽  
Vol 10 (3) ◽  
pp. 1105-1105
Author(s):  
Geonwook Yoo ◽  
Heekyeong Park ◽  
Minjung Kim ◽  
Won Geun Song ◽  
Seokhwan Jeong ◽  
...  
Keyword(s):  
Real Time ◽  
Point Of Care ◽  
Electrical Detection ◽  
Point Of Care Diagnostics

A facile and real-time spectroscopic method for biofluid analysis in point-of-care diagnostics

Bioanalysis ◽  
2013 ◽  
Vol 5 (15) ◽  
pp. 1853-1861 ◽  
Author(s):  
Rajesh Kumar ◽  
Gajendra P Singh ◽  
Ishan Barman ◽  
Narahara Chari Dingari ◽  
Ghulam Nabi
Keyword(s):  
Real Time ◽  
Point Of Care ◽  
Spectroscopic Method ◽  
Point Of Care Diagnostics

Reusable Surface Molecular Imprint Biosensors Aided by Naturally Occurring Surface Roughness Indices for Point-of-Care Diagnostics

MRS Advances ◽  
2019 ◽  
Vol 4 (22) ◽  
pp. 1299-1308 ◽  
Author(s):  
Yehoshua Auerbach ◽  
Rebecca Isseroff ◽  
Nicholas Clayton ◽  
Miguel Hulyalkar ◽  
Andrew Todt ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Imprinting ◽  
Point Of Care ◽  
High Sensitivity ◽  
Cost Effective ◽  
Stem Cell Differentiation ◽  
Diverse Range ◽  
Point Of Care Diagnostics ◽  
Molecular Imprint ◽  
Detection Of Biomarkers

ABSTRACTWe have shown that molecular imprinting (MI) technology can be used to produce sensitive, robust, cost-effective biosensing systems with a real-time electrochemical readout that can be utilized for point of care diagnostics. Real time detection of biomarkers is essential when rapid, critical decisions need to be made, such as in situations where public health is threatened. Our biosensor has high sensitivity compared to standard methods like ELISA, and results are obtained within minutes, using inexpensive, accessible potentiometric readout technology. These biosensors utilize surface molecular imprinting of a self-assembling monolayer of hydroxy-terminated alkanethiol chains which form a crystalline ‘lock-and-key’ structure around a target analyte, allowing the sensors to detect and differentiate between bio-macromolecules of similar size and shape with high selectivity and sensitivity. The sensors are extremely versatile and able to detect a diverse range of molecules of varied chemical composition and structure. To fully exploit the sensors’ advantages, especially in remote, economically disadvantaged areas, it is important to quantify their durability and reusability. Biosensor chips were created to test the viability of hemoglobin detection and to evaluate the potential for sensor reusability when washed after detection testing. The successful readsorption of hemoglobin even after washing, accompanied by cyclic voltammetry data indicating the preservation of the SAM, indicate that these biosensors are reusable, significantly augmenting the device’s value. Potential applications include the analysis of complex chemical and biological processes such as stem cell differentiation and on-the-spot detection of diseases such as Zika.

scholarly journals Evaluation of a Hydrogel-Based Diagnostic Approach for the Point-of-Care Based Detection of Neisseria gonorrhoeae

Antibiotics ◽  
2018 ◽  
Vol 7 (3) ◽  
pp. 70 ◽  
Author(s):  
Sumudu Perera ◽  
Ali Taheri ◽  
Nurul Khan ◽  
Rajinder Parti ◽  
Stephanie Stefura ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Sensitivity And Specificity ◽  
Point Of Care ◽  
Pcr Detection ◽  
Diagnostic Approach ◽  
Rt Pcr ◽  
Neisseria Species ◽  
Point Of Care Test

Eleven primer pairs were developed for the identification of Neisseria gonorrhoeae. The sensitivity and specificity of these primers were evaluated by Real Time (RT)-PCR melt curve analyses with DNA from 145 N. gonorrhoeae isolates and 40 other Neisseria or non-Neisseria species. Three primer pairs were further evaluated in a hydrogel-based RT-PCR detection platform, using DNA extracted from 50 N. gonorrhoeae cultures. We observed 100% sensitivity and specificity in the hydrogel assay, confirming its potential as a point-of-care test (POCT) for N. gonorrhoeae diagnosis.

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