Ultrafast VCSEL-based plasmonic polymerase chain reaction with real-time label-free amplicon detection for point-of-care diagnostics

2020 ◽  
Author(s):  
Padideh Mohammadyousef ◽  
Gideon Uchehara ◽  
Miltiadis Paliouras ◽  
Mark Trifiro ◽  
Andrew G. Kirk
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Point Of Care ◽  
Label Free ◽  
Chain Reaction ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain

Ultrafast plasmonic and real-time label-free polymerase chain reaction

2020 ◽  
Author(s):  
Padideh Mohammadyousef ◽  
Miltiadis Paliouras ◽  
Mark Trifiro ◽  
Andrew G. Kirk
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Label Free ◽  
Chain Reaction ◽  
Polymerase Chain

Nanostructured biochip for label-free and real-time optical detection of polymerase chain reaction

2010 ◽  
Vol 661 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Ha Minh Hiep ◽  
Kagan Kerman ◽  
Tatsuro Endo ◽  
Masato Saito ◽  
Eiichi Tamiya
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Optical Detection ◽  
Label Free ◽  
Chain Reaction ◽  
Polymerase Chain

Direct urine polymerase chain reaction for chlamydia and gonorrhoea: a simple means of bringing high-throughput rapid testing to remote settings?

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 299 ◽  
Author(s):  
Frashta Rahimi ◽  
Namraj Goire ◽  
Rebecca Guy ◽  
John M. Kaldor ◽  
James Ward ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Point Of Care ◽  
Resistance Mechanisms ◽  
Rapid Screening ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Urine Testing

Background Rapid point-of-care tests (POCTs) for chlamydia (Chlamydia trachomatis) and gonorrhoea (Neisseria gonorrhoeae) have the potential to confer health benefits in certain populations even at moderate sensitivities; however, suitable POCTs for these organisms are currently lacking. Methods: In this study, we investigated the use of direct urine polymerase chain reaction (PCR), with the view of implementing a simplified PCR strategy for high-throughput chlamydia and gonorrhoea screening in remote settings. Briefly, a simple dilution of the urine was performed before adding it directly to a real-time PCR reaction. The method was evaluated using 134 stored urine specimens that had been submitted for chlamydia and gonorrhoea testing and had been tested using a commercial C. trachomatis and N. gonorrhoeae PCR method. These included samples that were PCR-positive for chlamydia (n = 87), gonorrhoea (n = 16) or both (n = 2). Direct urine testing was conducted using previously described in-house real-time PCR methods for C. trachomatis and N. gonorrhoeae as well as for recognised N.gonorrhoeae antimicrobial resistance mechanisms. Results: The overall sensitivities and specificities of the direct urine PCR were 78% and 100% for chlamydia, and 83% and 100% for gonorrhoea. N.gonorrhoeae penicillin and quinolone resistance mechanisms were characterised in 14 of the 18 N. gonorrhoeae-positive samples. Conclusions: The results of this study show that the simplified PCR strategy may be a feasible approach for rapid screening and improving chlamydia and gonorrhoea treatment in remote settings.

Plasmonic and label-free real-time quantitative PCR for point-of-care diagnostics

The Analyst ◽  
2021 ◽  
Author(s):  
Padideh Mohammadyousef ◽  
Miltiadis Paliouras ◽  
Mark Trifiro ◽  
Andrew Kirk
Keyword(s):  
Pathogen Detection ◽  
Point Of Care ◽  
Medical Community ◽  
Molecular Diagnostic ◽  
Label Free ◽  
Chain Reaction ◽  
Real Time Quantitative Pcr ◽  
Point Of Care Diagnostics ◽  
Polymerase Chain ◽  
Infectious Pathogen

In response to the world’s medical community need for accurate and immediate infectious pathogen detection, many researchers have focused on adapting the standard molecular diagnostic method of polymerase chain reaction...

scholarly journals Real time label-free monitoring of plasmonic polymerase chain reaction products

2019 ◽  
Author(s):  
Gideon Uchehara ◽  
Andrew G. Kirk ◽  
Mark Trifiro ◽  
Miltiadis Paliouras ◽  
Padideh Mohammadyousef
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Label Free ◽  
Reaction Products ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A microheater on polyimide substrate for hand-held realtime microfluidic polymerase chain reaction amplification

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Dae-Sik Lee ◽  
Ok Ran Choi ◽  
Yujin Seo
Keyword(s):  
Polymerase Chain Reaction ◽  
Power Consumption ◽  
Low Power ◽  
Real Time ◽  
High Speed ◽  
Point Of Care ◽  
Polymerase Chain Reaction Amplification ◽  
Dna Amplification ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract The development of a DNA microfluidic device with a high speed, low power, and low reagent volume is very critical for real-time genotyping and diagnosis in point-of-care applications. This paper reports a polymer-based thermal cycler for a handheld and battery-powered polymerase chain reaction (PCR) system using a polyimide (PI) film-based micro-fabricated heater module and polymer film microfluidic chambers of 10 μL, with a handheld and low power consumption, compared to state of the art. It took 21 min for 40 thermal cycling for DNA amplification and a maximum power consumption of 0.6 W. The microheater on PI film substrate fabricated and real-time quantification of deoxyribonucleic acid (DNA) using the heater in hand-held sizes experimentally shown here. The device would be applicable for on-site molecular diagnostics.

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