scholarly journals Effect of sinapic acid on 1,2 dimethylhydrazine induced aberrant crypt foci, biotransforming bacterial enzymes and circulatory oxidative stress status in experimental rat colon carcinogenesis

2015 ◽  
Vol 116 (09) ◽  
pp. 560-566
Author(s):  
C. Balaji ◽  
J. Muthukumaran ◽  
N. Nalini
Keyword(s):  
Oxidative Stress ◽  
Sinapic Acid ◽  
Colon Carcinogenesis ◽  
Aberrant Crypt Foci ◽  
Rat Colon ◽  
Bacterial Enzymes ◽  
Oxidative Stress Status

scholarly journals Annona crassiflora Mart. fruit pulp effects on biochemical parameters and rat colon carcinogenesis

2013 ◽  
Vol 37 (4) ◽  
pp. 343-349 ◽  
Author(s):  
Vinícius Paula Venâncio ◽  
Eric Batista Ferreira ◽  
Maísa Ribeiro Pereira Lima Brigagão ◽  
Fernanda Borges de Araújo Paula ◽  
Luis Fernando Barbisan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cellular Localization ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Colon Carcinogenesis ◽  
Aberrant Crypt Foci ◽  
Nuclear Antigen ◽  
Rat Colon ◽  
Commercial Diet

A. crassiflora Mart. a Brazilian savannah fruit, is a source of phytochemical compounds that possess a wide array of biological activities, including free radical scavenging. This native fruit proved to potentialize the mutagenic process in previous in vivo investigations. The aim of the present study was to investigate the effects of A. crassiflora Mart. pulp intake on colonic cell proliferation and on the development of Aberrant Crypt Foci (ACF) in male Wistar rats. The animals were fed with either a commercial diet or a diet supplemented with A. crassiflora Mart. pulp mixed in 1%, 10% or 20% (w/w) for 4 weeks or 20 weeks. The carcinogen 1,2-dimethylhydrazine dihydrochloride (4 doses, 40 mg kg-1 each) was used to induce colonic ACF. After euthanasia, the blood, liver and colon samples were collected for biochemical determinations, oxidative stress or ACF development analysis, respectively. Immunohistochemical analyses of the colonic mucosa were performed using antibodies against proliferating cell nuclear antigen (PCNA) in normal-appearing colonic crypt and β-catenin in ACF. There was no ACF development in the colon from groups treated with A. crassiflora Mart. pulp. Also, the biochemical and oxidative stress analysis, PCNA labeling and ACF development (number, multiplicity or cellular localization of β-catenin) were unchanged as a result of marolo pulp intake. Thus, the present results suggest that A. crassiflora Mart. pulp intake did not exert any protective effect in the colon carcinogenesis induced by DMH in rats.

Styrax camporum, a typical species of the Brazilian cerrado, attenuates DNA damage, preneoplastic lesions and oxidative stress in experimental rat colon carcinogenesis

2021 ◽  
pp. 1-11
Author(s):  
Pollyanna Francielli De Oliveira ◽  
Luis Fernando Leandro ◽  
Ricardo Andrade Furtado ◽  
Natália Helen Ferreira ◽  
Patrícia Mendonça Pauletti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Colon Carcinogenesis ◽  
Rat Colon ◽  
Brazilian Cerrado ◽  
Preneoplastic Lesions ◽  
Typical Species ◽  
And Oxidative Stress

Chemopreventive effect of sinapic acid on 1,2-dimethylhydrazine-induced experimental rat colon carcinogenesis

2014 ◽  
Vol 33 (12) ◽  
pp. 1253-1268 ◽  
Author(s):  
C Balaji ◽  
J Muthukumaran ◽  
N Nalini
Keyword(s):  
Sinapic Acid ◽  
Colon Carcinogenesis ◽  
Thiobarbituric Acid ◽  
Experimental Period ◽  
Rat Colon ◽  
Uridine Diphosphate ◽  
Glutathione S Transferase ◽  
Herbal Plants ◽  
Chemopreventive Effect ◽  
Dt Diaphorase

Sinapic acid (SA) is a naturally occurring phenolic acid found in various herbal plants which is attributed with numerous pharmacological properties. This study was aimed to investigate the chemopreventive effect of SA on 1,2-dimethylhydrazine (DMH)-induced rat colon carcinogenesis. Rats were treated with DMH injections (20 mg kg−1 bodyweight (b.w.) subcutaneously once a week for the first 4 consecutive weeks and SA (20, 40 and 80 mg kg−1 b.w.) post orally for 16 weeks. At the end of the 16-week experimental period, all the rats were killed, and the tissues were evaluated biochemically. Our results reveal that DMH alone treatment decreased the levels/activities of lipid peroxidation by-products such as thiobarbituric acid reactive substances, conjugated dienes and antioxidants such as superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and reduced glutathione in the intestine and colonic tissues which were reversed on supplementation with SA. Moreover, the activities of drug-metabolizing enzymes of phase I (cytochrome P450 and P4502E1) were enhanced and those of phase II (glutathione- S-transferase, DT-diaphorase and uridine diphosphate glucuronosyl transferase) were diminished in the liver and colonic mucosa of DMH alone-treated rats and were reversed on supplementation with SA. All the above changes were supported by the histopathological observations of the rat liver and colon. These findings suggest that SA at the dose of 40 mg kg−1 b.w. was the most effective dose against DMH-induced colon carcinogenesis, and thus, SA could be used as a potential chemopreventive agent.

Lack of chemoprevention of dietary Agaricus blazei against rat colonic aberrant crypt foci

2008 ◽  
Vol 27 (6) ◽  
pp. 505-511 ◽  
Author(s):  
L Ziliotto ◽  
LF Barbisan ◽  
MAM Rodrigues
Keyword(s):  
Folk Medicine ◽  
Colon Carcinogenesis ◽  
Distal Colon ◽  
Edible Mushroom ◽  
Aberrant Crypt Foci ◽  
Nuclear Antigen ◽  
Rat Colon ◽  
Protective Effects ◽  
Agaricus Blazei ◽  
Initiation Stage

The mushroom Agaricus blazei ( Ab) has been widely used in folk medicine to treat various diseases including cancer. No information is available on its possible protective effects on the development of colon cancer. The potential blocking effect of Ab intake on the initiation stage of colon carcinogenesis was investigated in a short-term (4-week) bioassay using aberrant crypt foci (ACF) as biomarker. Male Wistar rats were given four subcutaneous injections of the carcinogen 1,2-dimethylhydrazine (DMH, 40 mg/kg bw, twice a week), during 2 weeks to induce ACF. The diet containing Ab at 5% was given 2 weeks before and during carcinogen treatment to investigate the potential beneficial effects of this edible mushroom on DMH-induced ACF. All groups were killed at the end of the fourth week. The colons were analyzed for ACF formation in 1% methylene blue whole-mount preparations and for cell proliferation in histological sections immunohistochemically stained for the proliferating cell nuclear antigen (PCNA). All DMH-treated rats developed ACF mainly in the middle and distal colon. Agaricus blazei intake at 5% did not alter the number of ACF induced by DMH or the PCNA indices in the colonic mucosa. Thus, the results of the present study did not confirm a chemopreventive activity of Ab on the initiation stage of rat colon carcinogenesis.

Dietary folate protects against azoxymethane-induced aberrant crypt foci development and oxidative stress in rat colon

2011 ◽  
Vol 236 (9) ◽  
pp. 1005-1011 ◽  
Author(s):  
Khalid S Al-Numair ◽  
Mostafa I Waly ◽  
Amanat Ali ◽  
Mohamed M Essa ◽  
Mohamed F Farhat ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aberrant Crypt Foci ◽  
Rat Colon ◽  
Dietary Folate ◽  
And Oxidative Stress

scholarly journals Modulating effect ofd-carvone on 1,2-dimethylhydrazine-induced pre-neoplastic lesions, oxidative stress and biotransforming enzymes, in an experimental model of rat colon carcinogenesis

2013 ◽  
Vol 46 (6) ◽  
pp. 705-720 ◽  
Author(s):  
R. Vinothkumar ◽  
M. Sudha ◽  
P. Viswanathan ◽  
J. Kabalimoorthy ◽  
T. Balasubramanian ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Experimental Model ◽  
Colon Carcinogenesis ◽  
Rat Colon ◽  
Neoplastic Lesions
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