scholarly journals Livestock-associated methicillin-resistant Staphylococcus aureus in Korea: antimicrobial resistance and molecular characteristics of LA-MRSA strains isolated from pigs, pig farmers, and farm environment

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Seung Hyun Back ◽  
Hong Sik Eom ◽  
Haeng Ho Lee ◽  
Gi Yong Lee ◽  
Kun Taek Park ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Molecular Characteristics ◽  
Methicillin Resistant ◽  
Mrsa Strains

Occurrence of Methicillin-Resistant Staphylococcus aureus in Ready-to-Eat Raw Fish from Japanese Cuisine Restaurants in Salvador, Brazil

2020 ◽  
Vol 83 (6) ◽  
pp. 991-995
Author(s):  
JOELZA SILVA CARVALHO ◽  
ANTENOR FERREIRA LEAL NETO ◽  
ISABELA MACIEL MELO ◽  
LUANA MILEN VARJÃO ◽  
CARLOS ALBERTO DAS NEVES ANDRADE ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Antimicrobial Resistance Profile ◽  
Fish Samples ◽  
High Prevalence ◽  
Disk Diffusion Assay ◽  
Laboratory Procedures ◽  
Mrsa Strains

ABSTRACT The presence of methicillin-resistant Staphylococcus aureus (MRSA) strains in food products is a major issue for food safety. The present study was conducted to evaluate the occurrence and antimicrobial resistance profile of S. aureus, focusing on MRSA isolates, in ready-to-eat sashimi from Japanese restaurants in Salvador, Brazil. A total of 127 sashimi samples were collected directly from the take-out service in 16 restaurants. The staphylococcal isolates were identified morphologically and biochemically with standard laboratory procedures. S. aureus isolates were tested with a disk diffusion assay against seven antibiotics, and the cefoxitin and oxacillin were used to identify MRSA strains. Isolates with the MRSA phenotype were confirmed with a PCR assay. S. aureus was found in 73% of the sashimi samples, including sashimi from tuna (75.5% of samples) and salmon (72.5% of samples). Among those positive samples, 37% were contaminated with MRSA strains, found among 38.8% of salmon sashimi and 34.0% of tuna sashimi. Penicillin resistance was the most common type of antimicrobial resistance, found in 65.5% of the sashimi samples, followed by resistance to tetracycline (22.5%), erythromycin (16.0%), and ciprofloxacin (3.2%). Only two S. aureus isolates collected from different fish samples and restaurants had presumed resistance to vancomycin. The high prevalence of S. aureus and MRSA in these sashimi samples indicates a potential risk for foodborne disease, especially MRSA, spreading in the community. HIGHLIGHTS

scholarly journals Antimicrobial Resistance and Molecular Characterization of Methicillin-Resistant Staphylococcus aureus Isolated from Slaughtered Pigs and Pork in the Central Region of Thailand

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 206
Author(s):  
Wimonrat Tanomsridachchai ◽  
Kanjana Changkaew ◽  
Ruchirada Changkwanyeun ◽  
Watsawan Prapasawat ◽  
Apiradee Intarapuk ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Health Concern ◽  
Molecular Characteristics ◽  
P Value ◽  
Methicillin Resistant ◽  
Pork Samples ◽  
Slaughtered Pigs

Methicillin-resistant Staphylococcus aureus (MRSA) have been a major public health concern in humans. Among MRSA, livestock-associated (LA)-MRSA strains have always been associated with exposure to livestock or their products and have emerged in different countries globally. Although studies have identified LA-MRSA from healthy pigs and pork in Thailand, prevalence in slaughtered pigs is still unknown. In addition, there are few reports on the epidemiology and molecular characteristics of LA-MRSA in Thailand. Hence, this is the first report investigating the epidemiology and molecular characteristics of MRSA in individual slaughtered pigs and pork in Thailand. A total of 204 nasal swab and 116 retailed pork samples were collected from three slaughterhouses and four fresh markets, respectively. Individual samples were used for screening for MRSA and obtained isolates were examined for drug- resistance profiling for 12 antimicrobial agents of 10 drug classes. In addition, SCCmec typing and multi-locus sequence typing were conducted to obtain genotype profiles. MRSA were isolated from 11 and 52 nasal swab and pork samples, respectively. The prevalence was significantly higher in the pork than in the nasal swab samples (p-value < 0.05). A high prevalence of ST9-SCCmecIX and ST398-SCCmecV with high-level antimicrobial resistance from markets and slaughterhouses indicated the spreading of MRSA with these genotypes in the Thai swine processing chains and suggested the need for further investigation to determine a control.

Molecular Characteristics and Changing Trend of Methicillin-Resistant Staphylococcus Aureus From Invasive Infections between 2012 and 2018

2020 ◽  
Author(s):  
Yonghong Xiao ◽  
Yunying Zhu ◽  
Yuan Wang ◽  
Hao Xu ◽  
Tingting Xiao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lower Probability ◽  
Molecular Characteristics ◽  
Methicillin Resistant ◽  
Invasive Infections ◽  
Antimicrobial Susceptibility Tests ◽  
Changing Trend ◽  
Mrsa Strains ◽  
Susceptibility Tests

Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major global problem. The analysis of the molecular characteristics and changing trend of MRSA is essential for the control and treatment of diseases caused by the pathogen.Methods A total of 162 MRSA isolates from invasive infections between 2012 and 2018 were collected, molecular typing and antimicrobial susceptibility tests to explore its molecular epidemiologic change in a hospital were performed.Results All of the 162 MRSA isolates (86.4% HA-MRSA and 13.6% CA-MRSA) were divided into 16 different ST and 30 Spa types. The major STs were ST5 (96/162, 59.3%) and the predominant spa type was t311(83/162, 51.2%). Five SCCmec types were found and the most common SCCmec type was type II (101/162, 61.7%). The prevalence of ST5 MRSA gradually declined from 2014 to 2018 but the prevalence of ST59 MRSA significantly increased. At the same time, livestock-associated methicillin-resistant S.aureus ST239 and ST9 were detected. 28 isolates were Panton-valentine leucocidin (pvl) gene positive (28/162, 17.3%). The most prevalent pvl-positive clone was ST59-IVa-t437. Comparing with HA-MRSA, CA-MRSA had a lower probability of ST5 (9.1% vs, 67.1%, P=0.000) but a higher probability of ST59 (63.6% vs. 11.4%, P=0.000), not only that, it was more likely to carrying pvl-positive gene (36.4% vs. 14.3%, P=0.028).Conclusions The molecular types of MRSA were getting complex over time. ST5-II-t311 was the predominant clone of MRSA isolate with a downward incidence from 2012 to 2018. ST59 MRSA strains, which is thought community related strain are spreading into hospitals and has an upward incidence during the investigational period.

scholarly journals Tracking methicillin-resistant Staphylococcus aureus clones in a hospital in Southern Mexico

2020 ◽  
Vol 62 (2, Mar-Abr) ◽  
pp. 186
Author(s):  
Miguel Ángel Ortíz-Gil ◽  
Maria Elena Velazquez-Meza ◽  
Gabriela Echániz-Aviles ◽  
Javier Paul Mora-Domínguez ◽  
María Noemi Carnalla-Barajas ◽  
...  
Keyword(s):  
New York ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Hospital Environment ◽  
Molecular Characteristics ◽  
Southern Mexico ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Sequence Types ◽  
Sccmec Types

Objective. This study describes the clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains that were collected in the Hospital Regional de Alta Especialidad de Veracruz (HRV). Material and Methods. A total of 107 MRSA strains from individual patients were examined in the present study. The strains examined were collected between September 2009 and September 2010. The clinical and demographic characteristics of patients were analyzed; molecular typing by pulsed-field gel electrophoresis (PFGE), staphylococcal chromosomal cassette mec (SCCmec) typing and multilocus sequence typing (MLST) were used to characterize the isolates. Results. Two PFGE patterns (NY/J and IB) were identified with 4 and 3 subtypes respectively. The isolates analyzed showed two SCCmec types (I and II) and two sequence types (ST), ST247 and ST5 related with the Iberian and New York/Japan clones respectively. Conclusion. This study establishes the presence of two very important clonal lineages of MRSA: New York/Japan and Iberian clone in hospital environment.  

scholarly journals Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 395
Author(s):  
Katarina Pomorska ◽  
Vladislav Jakubu ◽  
Lucia Malisova ◽  
Marta Fridrichova ◽  
Martin Musilek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bloodstream Infections ◽  
Spa Typing ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Spa Types ◽  
Repeat Pattern

Staphylococcus aureus is one of the major causes of bloodstream infections. The aim of our study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from blood of patients hospitalized in the Czech Republic between 2016 and 2018. All MRSA strains were tested for antibiotic susceptibility, analyzed by spa typing and clustered using a Based Upon Repeat Pattern (BURP) algorithm. The representative isolates of the four most common spa types and representative isolates of all spa clonal complexes were further typed by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. The majority of MRSA strains were resistant to ciprofloxacin (94%), erythromycin (95.5%) and clindamycin (95.6%). Among the 618 strains analyzed, 52 different spa types were detected. BURP analysis divided them into six different clusters. The most common spa types were t003, t586, t014 and t002, all belonging to the CC5 (clonal complex). CC5 was the most abundant MLST CC of our study, comprising of 91.7% (n = 565) of spa-typeable isolates. Other CCs present in our study were CC398, CC22, CC8, CC45 and CC97. To our knowledge, this is the biggest nationwide study aimed at typing MRSA blood isolates from the Czech Republic.

Sign in / Sign up

Export Citation Format

Share Document