Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis

2017 ◽  
Vol 104 ◽  
pp. 328-335 ◽  
Author(s):  
Mehdi Goudarzi ◽  
Sima Sadat Seyedjavadi ◽  
Mohammad Javad Nasiri ◽  
Hossein Goudarzi ◽  
Raheleh Sajadi Nia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Molecular Characteristics ◽  
Methicillin Resistant ◽  
Mrsa Strains

Molecular Characteristics and Changing Trend of Methicillin-Resistant Staphylococcus Aureus From Invasive Infections between 2012 and 2018

2020 ◽  
Author(s):  
Yonghong Xiao ◽  
Yunying Zhu ◽  
Yuan Wang ◽  
Hao Xu ◽  
Tingting Xiao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lower Probability ◽  
Molecular Characteristics ◽  
Methicillin Resistant ◽  
Invasive Infections ◽  
Antimicrobial Susceptibility Tests ◽  
Changing Trend ◽  
Mrsa Strains ◽  
Susceptibility Tests

Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major global problem. The analysis of the molecular characteristics and changing trend of MRSA is essential for the control and treatment of diseases caused by the pathogen.Methods A total of 162 MRSA isolates from invasive infections between 2012 and 2018 were collected, molecular typing and antimicrobial susceptibility tests to explore its molecular epidemiologic change in a hospital were performed.Results All of the 162 MRSA isolates (86.4% HA-MRSA and 13.6% CA-MRSA) were divided into 16 different ST and 30 Spa types. The major STs were ST5 (96/162, 59.3%) and the predominant spa type was t311(83/162, 51.2%). Five SCCmec types were found and the most common SCCmec type was type II (101/162, 61.7%). The prevalence of ST5 MRSA gradually declined from 2014 to 2018 but the prevalence of ST59 MRSA significantly increased. At the same time, livestock-associated methicillin-resistant S.aureus ST239 and ST9 were detected. 28 isolates were Panton-valentine leucocidin (pvl) gene positive (28/162, 17.3%). The most prevalent pvl-positive clone was ST59-IVa-t437. Comparing with HA-MRSA, CA-MRSA had a lower probability of ST5 (9.1% vs, 67.1%, P=0.000) but a higher probability of ST59 (63.6% vs. 11.4%, P=0.000), not only that, it was more likely to carrying pvl-positive gene (36.4% vs. 14.3%, P=0.028).Conclusions The molecular types of MRSA were getting complex over time. ST5-II-t311 was the predominant clone of MRSA isolate with a downward incidence from 2012 to 2018. ST59 MRSA strains, which is thought community related strain are spreading into hospitals and has an upward incidence during the investigational period.

scholarly journals Tracking methicillin-resistant Staphylococcus aureus clones in a hospital in Southern Mexico

2020 ◽  
Vol 62 (2, Mar-Abr) ◽  
pp. 186
Author(s):  
Miguel Ángel Ortíz-Gil ◽  
Maria Elena Velazquez-Meza ◽  
Gabriela Echániz-Aviles ◽  
Javier Paul Mora-Domínguez ◽  
María Noemi Carnalla-Barajas ◽  
...  
Keyword(s):  
New York ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Hospital Environment ◽  
Molecular Characteristics ◽  
Southern Mexico ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Sequence Types ◽  
Sccmec Types

Objective. This study describes the clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains that were collected in the Hospital Regional de Alta Especialidad de Veracruz (HRV). Material and Methods. A total of 107 MRSA strains from individual patients were examined in the present study. The strains examined were collected between September 2009 and September 2010. The clinical and demographic characteristics of patients were analyzed; molecular typing by pulsed-field gel electrophoresis (PFGE), staphylococcal chromosomal cassette mec (SCCmec) typing and multilocus sequence typing (MLST) were used to characterize the isolates. Results. Two PFGE patterns (NY/J and IB) were identified with 4 and 3 subtypes respectively. The isolates analyzed showed two SCCmec types (I and II) and two sequence types (ST), ST247 and ST5 related with the Iberian and New York/Japan clones respectively. Conclusion. This study establishes the presence of two very important clonal lineages of MRSA: New York/Japan and Iberian clone in hospital environment.  

scholarly journals Antibiotic Resistance, spa Typing and Clonal Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Blood of Patients Hospitalized in the Czech Republic

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 395
Author(s):  
Katarina Pomorska ◽  
Vladislav Jakubu ◽  
Lucia Malisova ◽  
Marta Fridrichova ◽  
Martin Musilek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Czech Republic ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Bloodstream Infections ◽  
Spa Typing ◽  
The Czech Republic ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Spa Types ◽  
Repeat Pattern

Staphylococcus aureus is one of the major causes of bloodstream infections. The aim of our study was to characterize methicillin-resistant Staphylococcus aureus (MRSA) isolates from blood of patients hospitalized in the Czech Republic between 2016 and 2018. All MRSA strains were tested for antibiotic susceptibility, analyzed by spa typing and clustered using a Based Upon Repeat Pattern (BURP) algorithm. The representative isolates of the four most common spa types and representative isolates of all spa clonal complexes were further typed by multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing. The majority of MRSA strains were resistant to ciprofloxacin (94%), erythromycin (95.5%) and clindamycin (95.6%). Among the 618 strains analyzed, 52 different spa types were detected. BURP analysis divided them into six different clusters. The most common spa types were t003, t586, t014 and t002, all belonging to the CC5 (clonal complex). CC5 was the most abundant MLST CC of our study, comprising of 91.7% (n = 565) of spa-typeable isolates. Other CCs present in our study were CC398, CC22, CC8, CC45 and CC97. To our knowledge, this is the biggest nationwide study aimed at typing MRSA blood isolates from the Czech Republic.

Multiclonal outbreak of methicillin-resistant Staphylococcus aureus infections on a collegiate football team

2008 ◽  
Vol 137 (1) ◽  
pp. 85-93 ◽  
Author(s):  
A. J. HALL ◽  
D. BIXLER ◽  
L. E. HADDY
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Infection Risk ◽  
Physical Contact ◽  
Soft Tissue Infections ◽  
Risk Of Infection ◽  
Methicillin Resistant ◽  
Football Team ◽  
Mrsa Strains ◽  
Collegiate Football

SUMMARYAn outbreak of methicillin-resistant Staphylococcus aureus (MRSA) skin and soft tissue infections (SSTIs) occurred in a college football team in August 2006. Of 109 players on the team roster, 88 (81%) were interviewed during a cohort investigation. Twenty-five cases were identified, six of which were culture-confirmed. Available culture isolates were typed by pulsed-field gel electrophoresis (PFGE), which identified two different MRSA strains associated with the outbreak. Playing positions with the most physical contact (offensive linemen, defensive linemen, and tight ends) had the greatest risk of infection [risk ratio (RR) 5·1, 95% confidence interval (CI) 2·3–11·5. Other risk factors included recent skin trauma (RR 1·9, 95% CI 0·95–3·7), use of therapeutic hydrocollator packs (RR 2·5, 95% CI 1·1–5·7), and miscellaneous training equipment use (RR 2·1, 95% CI 1·1–4·1). The outbreak was successfully controlled through team education and implementation of improved infection-control practices and hygiene policies.

Multilocus Sequence Typing and Staphylococcal Protein A Typing Revealed Novel and Diverse Clones of Methicillin-Resistant Staphylococcus aureus in Seafood and the Aquatic Environment

2017 ◽  
Vol 80 (3) ◽  
pp. 476-481 ◽  
Author(s):  
V. Murugadas ◽  
C. Joseph Toms ◽  
Sara A. Reethu ◽  
K. V. Lalitha
Keyword(s):  
Staphylococcus Aureus ◽  
Aquatic Environment ◽  
Multilocus Sequence Typing ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Spa Typing ◽  
Methicillin Resistant ◽  
Staphylococcal Protein ◽  
Mrsa Strains

ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) has been a global health concern since the 1960s, and isolation of this pathogen from food-producing animals has been increasing. However, little information is available on the prevalence of MRSA and its clonal characteristics in seafood and the aquatic environment. In this study, 267 seafood and aquatic environment samples were collected from three districts of Kerala, India. Staphylococcal protein A (spa) typing and multilocus sequence typing (MLST) was performed for 65 MRSA strains isolated from 20 seafood and aquatic environment samples. The MRSA clonal profiles were t657-ST772, t002-ST5, t334-ST5, t311-ST5, t121-ST8, t186-ST88, t127-ST1, and two non-spa assignable strains. Whole spa gene sequence analysis along with MLST confirmed one strain as t711-ST6 and another as a novel MRSA clone identified for the first time in seafood and the aquatic environment with a t15669 spa type and a new MLST profile of ST420-256-236-66-82-411-477. The MRSA strains were clustered into five clonal complexes based on the goeBURST algorithm, indicating high diversity among MRSA strains in seafood and the aquatic environment. The novel clone formed a separate clonal complex with matches to three loci. This study recommends large-scale spa typing and MLST of MRSA isolates from seafood and the aquatic environment to determine the prevalence of new MRSA clones. This monitoring process can be useful for tracing local spread of MRSA isolates into the seafood production chain in a defined geographical area.

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