A rapid method to detect Cryptolestes ferrugineus (Coleoptera: Cucujidae) larvae in stored grain

2002 ◽  
Vol 82 (3) ◽  
pp. 591-597 ◽  
Author(s):  
J. M. Minkevich ◽  
C. J. Demianyk ◽  
N. D. G. White ◽  
D. S. Jayas ◽  
B. Timlick
Keyword(s):  
Extraction Efficiency ◽  
Survival Rates ◽  
Detection Methods ◽  
Heat Sources ◽  
Cryptolestes Ferrugineus ◽  
Stored Grain ◽  
Potential Alternative ◽  
Alternative Detection ◽  
Gradient Principle ◽  
Seed Coats

Modifications of three detection methods, based on the heat-gradient principle employed by the Berlese funnel method were investigated to extract larvae of the rusty grain beetle, Cryptolestes ferrugineus (Stephens), developing under the seed coats of infested kernels in grain samples. One-kilogram samples of wheat, barley and corn were artificially infested with rusty grain beetle eggs, resulting in survival rates (to larval stage) of 71.1 ± 14.4, 58.9 ± 14.3, and 24.7 ± 11.8%, respectively. Sets of 10 infested kernels containing different-aged larvae (10 individuals × 4 instars) were added to 1-kg samples of hydrated grain, then heated on screens beneath heat sources (lights) at several heights in three different containers (Berlese funnel, with a 7-cm-deep grain layer, or square and rectangular screened boxes with a grain layer several kernels deep). There were no significantly different extraction rates between the rectangular and square containers for all heating trials. A larval extraction rate of 31% was produced by 1-h trials with wheat (15% moisture content wet basis) 5.5 cm below the lamp bank with a thermostat set at 50°C. This matched the efficiency of the Berlese funnel method (36% extraction in 6 h), but in much less time. Similar results were found for barley, bu t for corn the square and rectangle gave significantly better extraction than the Berlese funnel, although extraction efficiency was half that of wheat and barley. Considerably lower extraction rates were obtained from trials that did not use a thermostat. The results from this experiment show that there is a potential alternative detection method with the thermostatically controlled heating of a thin layer of grain that should be faster than the conventional 6-h Berlese funnel method. Key words: Cryptolestes ferrugineus, grain, larval extraction, Berlese funnel, grain monolayer

scholarly journals Geoneutrinos

2012 ◽  
Vol 2012 ◽  
pp. 1-34 ◽  
Author(s):  
Ondřej Šrámek ◽  
William F. McDonough ◽  
John G. Learned
Keyword(s):  
Continental Crust ◽  
Plate Tectonics ◽  
Detection Methods ◽  
Heat Sources ◽  
Radiogenic Heat ◽  
Interdisciplinary Field ◽  
The Earth ◽  
Directional Detection ◽  
Under Construction ◽  
Current Detection

Neutrino geophysics is an emerging interdisciplinary field with the potential to map the abundances and distribution of radiogenic heat sources in the continental crust and deep Earth. To date, data from two different experiments quantify the amount of Th and U in the Earth and begin to put constraints on radiogenic power in the Earth available for driving mantle convection and plate tectonics. New improved detectors are under construction or in planning stages. Critical testing of compositional models of the Earth requires integrating geoneutrino and geological observations. Such tests will lead to significant constraints on the absolute and relative abundances of U and Th in the continents. High radioactivity in continental crust puts limits on land-based observatories' capacity to resolve mantle models with current detection methods. Multiple-site measurement in oceanic areas away from continental crust and nuclear reactors offers the best potential to extract mantle information. Geophysics would benefit from directional detection and the detectability of electron antineutrinos from potassium decay.

AABH (aspartyl [asparaginyl] β-hydroxylase) as a serum biomarker for breast cancer.

2020 ◽  
Vol 38 (5_suppl) ◽  
pp. 2-2
Author(s):  
Kiarash Moshiri
Keyword(s):  
Breast Cancer ◽  
Sandwich Elisa ◽  
Survival Rates ◽  
Elevated Serum ◽  
Disease Recurrence ◽  
The United States ◽  
Develop Breast Cancer ◽  
Detection Methods ◽  
Great Promise ◽  
Primary Tumors

2 Background: Breast cancer accounts for 27% of female cancers. One in every eight women will develop breast cancer in their lifetime. More than 220,000 new cases of breast cancer will be diagnosed in the United States in 2020. Five-year survival rates for breast cancer are 98% for localized disease, 83% for regionally spread and 26% for distantly spread cancer. However, 25% of all metastases occur more than 5 years after the initial diagnosis and thus survival rates decrease as time goes out to 10 years due to disease recurrence. Current detection methods rely on imaging modalities, including mammography and MRI. No serum bio-markers have been approved for either breast cancer screening or monitoring for disease recurrence, yet. In asymptomatic women in remission from breast cancer the guidelines of the major oncologic organizations only suggest annual mammography for detection of new primary tumors. Methods: We have investigated the utility of aspartyl (asparaginyl) β-hydroxylase (AABH) as a serum bio-marker for cancer. AABH has been detected by immunohistochemical staining (IHC) in a broad range of cancers including breast cancer. It has been detected by IHC in > 99% of tumor specimens tested (n > 2000) but is absent in adjacent non-affected tissue, and in tissue samples from non-affected individuals. This led to the development of a sandwich ELISA that reliably measures AABH in serum. Results: In the current study we have utilized the assay to quantify AABH levels in the sera of patients diagnosed with Breast Cancer compared to women free of disease. Increased levels of AABH were found in the serum of 99% of patients with breast cancer (n = 189). In women not known to have cancer, AABH was essentially undetectable in serum (n = 65, specificity = 96%). Serum from several women currently in remission subsequent to treatment for breast cancer were also analyzed and found to be negative for AABH. Conclusions: Thus, measurement of serum AABH levels has great promise as a diagnostic tool for breast cancer with potential application in monitoring for disease recurrence. Elevated serum AABH in conjunction with mammography and MRI may greatly facilitate earlier diagnosis of both primary and recurrent Breast Cancer.

scholarly journals Laboratory Confirmation of Lyme Disease

1991 ◽  
Vol 2 (2) ◽  
pp. 64-69 ◽  
Author(s):  
Tom G Schwan ◽  
Warren J Simpson ◽  
Patricia A Rosa
Keyword(s):  
Lyme Disease ◽  
Immunofluorescence Assay ◽  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Tests ◽  
Specificity And Sensitivity ◽  
Laboratory Confirmation ◽  
Staining Methods ◽  
Alternative Detection ◽  
Species Specific

Lyme disease can be confirmed in the laboratory by isolation or detection of its causative agent, a tick-borne spirocheteBorrelia burgdorferi,or by a diagnostic change in the titre of antibodies specific to the agent.B burgdorferican be isolated and cultivated in Barbour-Stoenner-Kelly II medium. It can be detected by light microscopy in tissue sections or, rarely, in blood smears using various staining methods. There is interest in the development of alternative detection methods, including identification of specific antigens ofB burgdorferiin the urine of suspected cases and demonstration of the presence of species-specific DNA using polymerase chain reaction assays. Currently, serological tests (indirect immunofluorescence assay, enzyme-linked immunosorbent assay and Western immunoblot) are the most practical and available methods for confirming Lyme disease. The quest to improve the specificity and sensitivity of serological tests – for example, through the use of specific recombinant antigens – continues.

FIELD EVALUATION OF A PHEROMONE TO DETECT ADULT RUSTY GRAIN BEETLES, CRYPTOLESTES FERRUGINEUS (COLEOPTERA: CUCUJIDAE), IN STORED GRAIN

1986 ◽  
Vol 118 (1) ◽  
pp. 1-8 ◽  
Author(s):  
S.R. Loschiavo ◽  
J. Wong ◽  
N.D.G. White ◽  
H.D. Pierce ◽  
J.H. Borden ◽  
...  
Keyword(s):  
Field Evaluation ◽  
Aggregation Pheromones ◽  
Cryptolestes Ferrugineus ◽  
Stored Grain ◽  
Baited Traps ◽  
Grain Beetles ◽  
Polyester Fibers

AbstractInsect-detection traps baited with the synthetic aggregation pheromones of the rusty grain beetle, Cryptolestes ferrugineus (Stephens), were inserted into grain stored in primary elevator annex bins and in farm bins, or suspended in partly full or empty bins and outside under the eaves of bins. The mixture of the synthetic pheromones 4,8-dimethyl-E,E-4,8-decadienolide (ferrulactone I) and (Z)-3-dodecen-11-olide (ferrulactone II) was released from hollow polyester fibers (0.203 mm ID) positioned in the interior of the insect-detection trap. In annex bins, the number of beetles found in baited traps after 1 week ranged from 0 to 7300. In these bins, more beetles were found in traps at a depth of 30–60 cm than at 150–250 cm, and in traps with 2 fibers containing pheromone rather than with 1 fiber. In farm bins, baited and unbaited traps were relatively ineffective at grain temperatures below 14 °C. More beetles were found in baited than in unbaited traps in farm bins but the numbers were small and the differences were not significant. Few beetles were found in traps suspended in partly full or empty farm bins and none in traps suspended outside under the eaves.

scholarly journals Efektivitas Ekstrak Tepung Testis Sapi dalam Alih Kelamin Ikan Nila, Oreochromis niloticus L. Melalui Teknik Perendaman (The Effectiveness of BTME (Bull Testes Meal Extract) in Sex-Reversal of Tilapia through Immersion Technique)

2014 ◽  
Vol 4 (1) ◽  
pp. 27-34
Author(s):  
Andri Iskandar ◽  
M. Zairin Junior ◽  
Harton Arfah
Keyword(s):  
Environmental Sustainability ◽  
Oreochromis Niloticus ◽  
Natural Compound ◽  
Negative Impact ◽  
Survival Rates ◽  
Sex Reversal ◽  
Male Fish ◽  
Potential Alternative ◽  
Survival And Growth ◽  
Synthetic Steroids

Synthetic steroids are commonly used to sex-reverse tilapia to produce male tilapia commercially, but gradually feared a negative impact on food security and environmental sustainability. The use of new natural compound is a potential alternative to be explored, one of which is bull testes meal extract (BTME). This study aimed to evaluate the effectiveness of BTME immersion and optimal concentrations for sex-reverse tilapia to produce monosex male tilapia, and its influence on survival and growth of fish. The study consisted of 5 treatments with 3 replications: immersion treatment with BTME concentration of 1 ml l-1 (A), 3 ml l-1 (B), 5 ml l-1 (C), immersion treatment in 17α-methyltestosterone of 500 µg l-1 (K+) and without hormone immersion treatment (K-). Hormone immersion was performed on tilapia larvae aged 4 and 7 days after hatching.The fish were then maintained for 60 days. The parameters observed were: the percentage of male tilapia and intersex tilapia, survival rates, and growth rate of fish. BTME immersion for sex-reverse the tilapia larvae have significant effect on masculinization of tilapia. The highest percentage of male fish was obtained on treatment C (85.56%) and was not significantly different from K (+) (81.11 %). Immersion of tilapia in BTME or 17α-MT did not affect the survival and growth of tilapia fish.Keywords: sex reversal, bull testes meal extract (BTME), tilapia

scholarly journals A review of some important viral diseases of wild boars

2021 ◽  
Vol 37 (4) ◽  
pp. 235-254
Author(s):  
Jakov Nisavic ◽  
Andrea Radalj ◽  
Nenad Milic ◽  
Aleksandar Zivulj ◽  
Damir Benkovic ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Control Measure ◽  
Survival Rates ◽  
African Swine Fever ◽  
Classical Swine Fever ◽  
Control Measures ◽  
Detection Methods ◽  
Virus Species ◽  
Wild Boars ◽  
Prevalence Studies

Wild boars are one of the widest-ranging mammals worldwide and represent reservoirs for many important viruses. Disease outbreaks in domestic swine are often described as a consequence of contact with wild boars, and traditional rearing conditions are a particular risk factor. Examples of such diseases include classical swine fever (CSF), African swine fever (ASF), Aujeszky?s disease (AD), and diseases caused by porcine circoviruses and parvoviruses. Some viral infections causing high mortality rates are easily noticeable and thus reported, though many viruses infecting wildlife are insidious impacting survival rates and reproduction in wild animals. Samples from wild boars for laboratory testing are usually collected postmortem and include various tissues or blood sera. The recovery of viable viruses during virus isolation depends on the virus species and the condition of the sample. Since this method does not yield timely results, most diagnostic procedures are based on PCR or antigen detection methods. Serological surveys are inexpensive and appropriate for prevalence studies. When interpreting the results of diagnostic tests, both virus and host characteristics, and the epizootiological situation must be accounted for. Disease control techniques such as fencing or feeding wild boars cause animal aggregation and give rise to population density which favors pathogen maintenance in the environment. Hunting reduces the number of susceptible animals and is helpful as an additional control measure and for sampling. Available data on infectious disease dynamics in wild boars is scarce, and constant knowledge improvement on pathogenesis, clinical symptoms, risk factors, and adequate control measures are required.

Comparison of various breast cancer detection methods with survival rates

2007 ◽  
Vol 25 (18_suppl) ◽  
pp. 17033-17033
Author(s):  
S. Misra ◽  
S. Tarr ◽  
D. Pratt
Keyword(s):  
Breast Cancer ◽  
Survival Time ◽  
Cancer Detection ◽  
Survival Rates ◽  
Staging System ◽  
Breast Cancer Detection ◽  
Detection Methods ◽  
Breast Cancer Patients ◽  
Estrogen Receptor Positivity

17033 Background: The role of mammography (MG) but not of breast self-exam (BSE) and clinical breast exam (CBE) in breast cancer detection and survival is well documented. This study compares the different methods of breast cancer detection and subsequent survival rates, analyzing the differences even within the same stage of the disease. Methods: Retrospective review of 1,259 patients was done using the hospital Tumor Registry data. Only patients with stage I, IIA and IIB are included and were diagnosed between April 1992 to December 2005 with follow up ranging from June 1993 to August 2006. The detection methods studied include BSE, CBE, MG and ultrasonography (USG). Parametric tests were conducted. Results: Mean age of the sample was 62 years (range 24–96). There were 293 BSE, 64 CBE, 885 MG, 17 USG detected breast cancer patients. Mean size of mass at presentation was 19 mm (range 1–110). Mean survival time for patients detected with breast cancer till August 2001 was 76 months (range 1–163). 67% patients survived 5 years or more and 12% survived more than 10 years. Mean survival for BSE/CBE and MG/USG group was 43 and 57 months respectively. This difference in survival is significant p< .05; The average survival time by stages I, IIA, IIB for BSE was 47, 45, 38 months, for CBE it was 43, 39, 51 months, for MG it was 57, 59, 50 months and for USG group it was 52, 47, 95 months respectively. Even within the same stage, the method of detection affected survival with the BSE and CBE group having less survival rates (Tukey Test mean difference 0.54, 95% C.I 42–66 and 0.38, 95% C.I 15–61) respectively than the MG group. Survival time also positively correlated with cancer recurrence (r =.7), family history (r = .06) and negatively correlated with age (r = -.09), size of tumor (r = -.09), estrogen receptor positivity status (r = -.06) all with (p < 0.05). We believe this study underestimates overall survival rate as the last follow up date was taken as an end point and also the survival rates are not disease specific survival. Conclusions: MG/USG group show higher survival rates compared to BSE/CBE across the early stages of breast cancer. Even within the same stage, the method of detection affects survival with MG/USG detected cases having more favorable outcomes. May be our current staging system for breast cancer is inadequate and needs revision. No significant financial relationships to disclose.

Extended Enrichment Procedures Can Be Used To Define False-Negative Probabilities for Cultural Gold Standard Methods for Salmonella Detection, Facilitating Comparisons between Gold Standard and Alternative Methods

2020 ◽  
Vol 83 (6) ◽  
pp. 1030-1037
Author(s):  
GENEVIEVE SULLIVAN ◽  
XIAODONG GUO ◽  
JEFFREY I. TOKMAN ◽  
SHERRY ROOF ◽  
ALJOSA TRMCIC ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Water Activity ◽  
Gold Standard ◽  
False Negative ◽  
Alternative Methods ◽  
Detection Methods ◽  
Pet Food ◽  
Probable Number ◽  
Alternative Detection ◽  
Contamination Levels

ABSTRACT Evaluation of alternative detection methods for foodborne pathogens typically involves comparisons against a “gold standard” culture method, which may produce false-negative (FN) results, particularly under worst-case scenarios such as low contamination levels, difficult-to-detect strains, and challenging food matrices (e.g., matrices with a water activity of &lt;0.6). We used extended enrichment times (up to 72 h for both primary and secondary enrichments) to evaluate a gold standard method for Salmonella detection (the U.S. Food and Drug Administration Bacteriological Analytical Manual [BAM] method) in two low-water-activity foods (dry pet food and chocolate) inoculated at low contamination levels (most probable number ca. 1/25 g) with five Salmonella strains. Strains were selected to include those with a poor ability to grow in enrichment media. Among the 100 pet food and 100 chocolate samples tested, 53 and 50, respectively, were positive with the standard BAM method, and 57 and 59, respectively, were positive with the extended BAM method. Thus, the FN probabilities for the standard BAM method were 7% for pet food and 15% for chocolate. An alternative enzyme immunoassay method for detection of Salmonella in chocolate produced FN probabilities of 6 and 20% when compared against the standard and extended BAM methods, respectively. Detection of Salmonella Mississippi was significantly reduced with the alternative method (P = 0.023) compared with the extended BAM method. We calculated a composite reference standard to further define FN probabilities based on variable results from multiple assays (the standard BAM, extended BAM, and alternative methods). Based on this standard, the enzyme immunoassay for Salmonella detection in chocolate had a 28% FN probability and the standard and extended BAM methods had 23 and 9% FN probabilities, respectively. These results provide a framework for how inclusion of extended enrichment times can facilitate evaluation of alternative detection methods. HIGHLIGHTS

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