SULFUR ISOTOPE FRACTIONATION DURING THE OXIDATION OF ELEMENTAL SULFUR BY THIOBACILLI IN A SOLONETZIC SOIL

1982 ◽  
Vol 62 (1) ◽  
pp. 105-110 ◽  
Author(s):  
R. G. L. McCREADY ◽  
H. R. KROUSE
Keyword(s):  
Soil Profile ◽  
Oxidation Rate ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Elemental Sulfur ◽  
Isotopic Analysis ◽  
Thiobacillus Thioparus ◽  
Sulfur Isotope Fractionation ◽  
Solonetzic Soil ◽  
S Oxidation

The oxidation rate of elemental S added to a Solonetzic soil was compared between an uninoculated soil and soils inoculated with two species of thiobacilli. Twelve weeks after S was added, 24% was oxidized to soluble S anions in the uninoculated soil, 34% in soil inoculated with Thiobacillus thiooxidans, and 84% in soil inoculated with Thiobacillus thioparus. The SO42− produced during S oxidation by thiobacilli was enriched in the lighter isotope by an average of 1‰ in comparison with the substrate S. Therefore, 34S-enriched S could be applied to Solonetzic soils and the migration of biologically produced H2SO4 into the soil profile may be followed by isotopic analysis of the soil sulfate.

Stable isotope fractionation by Clostridium pasteurianum. 5. Effect of SeO42− on the physiology and associated sulfur isotope fractionation during SO32− and SO42− reductions

1982 ◽  
Vol 28 (3) ◽  
pp. 325-333 ◽  
Author(s):  
G. I. Harrison ◽  
E. J. Laishley ◽  
H. R. Krouse
Keyword(s):  
Stationary Phase ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Reductase Activity ◽  
Clostridium Pasteurianum ◽  
Elemental Selenium ◽  
Whole Cells ◽  
Stable Isotope Fractionation ◽  
Sulfur Isotope Fractionation ◽  
Inverse Isotope Effect

The addition of 1 mM SeO42− significantly affected the physiology and metabolism of Clostridium pasteurianum growing on SO42− in the following ways: (1) the generation time was increased, essentially producing a biphasic growth curve, (2) cells became elongated and chains formed, (3) no H2S was liberated during the stationary phase, (4) assimilatory SO32− reductase activity was decreased, (5) ferredoxin levels decreased by a factor of 4. The effects of 1 mM SeO42− on Clostridium pasteurianum growing on SO32− were comparatively minor.H2S evolution in the stationary phase decreased by a factor of 2 and the δ34S maximum in the inverse isotope effect pattern occurred at a slightly lower percent H2S evolution. The deleterious effects of SeO42− addition were less pronounced than those associated with SeO32− addition. SeO32− but not SeO42− was reduced to elemental selenium by both whole cells and crude extracts.

Sulfur Isotope Fractionation by Sulfate-Reducing Microbes Can Reflect Past Physiology

2018 ◽  
Vol 52 (7) ◽  
pp. 4013-4022 ◽  
Author(s):  
André Pellerin ◽  
Christine B. Wenk ◽  
Itay Halevy ◽  
Boswell A. Wing
Keyword(s):  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Sulfate Reducing ◽  
Sulfur Isotope Fractionation

The effect of pantothenate on sulfate metabolism and sulfur isotope fractionation by Saccharomyces cerevisiae

1979 ◽  
Vol 25 (10) ◽  
pp. 1139-1144 ◽  
Author(s):  
R. G. L. McCready ◽  
G. A. Din ◽  
H. R. Krouse
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Sulfate Reduction ◽  
Lipid Content ◽  
Isotope Fractionation ◽  
Sulfur Isotope ◽  
Unsaturated Fatty Acids ◽  
Cellular Lipid ◽  
Sulfur Isotope Fractionation ◽  
Sulfate Metabolism

Growth of Saccharomyces cerevisiae in minimal salts – glucose – SO42− medium with varying concentrations of pantothenate (0–1000 μg/L) produced changes in the cellular lipid content and in the ratio of saturated to unsaturated fatty acids. Substantial differences in SO42−diffusion were also observed with changes in pantothenate concentration. During sulfate reduction, the δ34S value of the evolved sulfide varied with the pantothenate concentration ranging from −31‰ in the absence of pantothenate to 0‰ at 400−1000 μg/L pantothenate. The isotope selectivity is related to the effect of pantothenate concentration on cellular metabolism.

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