SUPERIOR PERFORMANCE OF THE HUP−Bradyrhizobium japonicum STRAIN 532C in ONTARIO SOYBEAN FIELD TRIALS
Field trials were conducted at different sites close to Guelph, Ontario, from 1981 to 1985, to evaluate the effects of Hup+ and Hup− strains on the performance of soybean (Glycine max L. Merrill) and to identify strains that could support high soybean yields under Ontario conditions. Each year six individual strains, Nitragin Soil Implant granular inoculant and an uninoculated control were compared for their effect on soybean yield. Soil at each experimental site was relatively free of indigenous B. japonicum as exhibited by an average of less than one nodule per plant from the uninoculated control plots. Inoculation with Hup+ or Hup− strains of B. japonicum caused similar average soybean seed yield. Of the inoculants tested (strains as well as commercial inoculant), the Hup− strain, 532C, also known as strain 61A152, supported the highest yields in 4 of the 5 yr. Omitting 1982 data, when soil available N was high and the uninoculated control had yields equivalent to inoculated plots, 532C treatments averaged 3.08 t ha−1 compared to 2.70 for 61A89, 2.84 for 61A133, 2.83 for the commercial inoculant and 1.96 for the uninoculated control. The consistent performance of 532C across years and locations suggested major advantages for this strain. Strain 532C is now being used as a single strain in five of the seven inoculants sold in Ontario.Key words: Soybean, inoculation, Hup+, seed yield