ISOZYME CHARACTERIZATION OF KENTUCKY BLUEGRASS CULTIVARS

1985 ◽  
Vol 65 (4) ◽  
pp. 985-994 ◽  
Author(s):  
N. F. WEEDEN ◽  
A. C. EMMO
Keyword(s):  
Cultivar Identification ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Polymorphism Analysis ◽  
Starch Gel Electrophoresis ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Starch Gel ◽  
Esterase Phenotypes

Twenty-two cultivars of Kentucky bluegrass (Poa pratensis L.) were examined for their glucose phosphate isomerase, triose phosphate isomerase, phosphoglucomutase, malate dehydrogenase, 6-phosphogluconate dehydrogenase and alpha-naphthyl esterase phenotypes. Polymorphism was observed in each isozyme system, and the first four systems were characterized for variation among and within the cultivars. Sixteen of the 22 cultivars could be uniquely identified by selecting isozymes which displayed clearly resolved bands and exhibited little or no intracultivar polymorphism. Analysis could be performed on single plants, permitting estimation of percent contamination should seed from different cultivars be mixed. Thus horizontal starch gel electrophoresis in combination with specific isozyme assays appears to offer a simple system for cultivar identification of Kentucky bluegrass seed.Key words: Cultivar identification, electrophoresis, isozymes, bluegrass (Kentucky)

scholarly journals Identifying Crabapple Cultivars by Isozymes

1995 ◽  
Vol 120 (5) ◽  
pp. 706-709 ◽  
Author(s):  
Robert D. Marquard ◽  
Charlotte R. Chan
Keyword(s):  
Alcohol Dehydrogenase ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Enzyme System ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Polymorphic Region ◽  
Banding Patterns ◽  
Phosphogluconate Dehydrogenase ◽  
Starch Gel

Forty-five crabapple (Malus spp.) cultivars were evaluated for 16 isozyme systems by starch gel electrophoresis. Of the 16 systems evaluated, 6 were useful in separating among cultivars. Enzyme systems used to distinguish among the cultivars included alcohol dehydrogenase, aspartate aminotransferase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucoisomerase, and shikimate dehydrogenase. Each enzyme system produced one well-resolved polymorphic region except for 6-phosphogluconate dehydrogenase, which produced two. Most crabapple selections could be identified when all six enzymes were evaluated. Alcohol dehydrogenase had the most diagnostic banding patterns useful for cultivar identification.

Enzyme variation in Eimeria species of the chicken

Parasitology ◽  
1975 ◽  
Vol 71 (3) ◽  
pp. 369-376 ◽  
Author(s):  
M. W. Shirley
Keyword(s):  
Species Differences ◽  
Eimeria Species ◽  
Starch Gel Electrophoresis ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Parasite Stage ◽  
Starch Gel ◽  
Biochemical Identification ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
First Time

A method for the biochemical identification of protozoa belonging to the genus Eimeria is described for the first time. Starch gel electrophoresis of the enzymes lactate dehydrogenase, glucose phosphate isomerase, 6-phosphogluconate dehydrogenase and glucose-6-phosphate dehydrogenase from parasite extracts revealed both intra- and inter-species differences when 11 strains representative of 6 species of Eimeria were examined. Oocysts were the most accessible parasite stage for investigation but sporozoites and merozoites of an embryo-adapted strain of E. tenella were also examined for enzyme activity.

Electrophoretic variation in cross-compatible wild diploid species of Cucumis

1987 ◽  
Vol 65 (4) ◽  
pp. 792-798 ◽  
Author(s):  
J. E. Staub ◽  
L. Fredrick ◽  
T. L. Marty
Keyword(s):  
Diploid Species ◽  
Starch Gel Electrophoresis ◽  
Shikimate Dehydrogenase ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Specific Variation ◽  
Starch Gel ◽  
Phylogenetic Affinities ◽  
Wild Diploid Species ◽  
Relative Similarity

Cotyledons of several collections of each taxon of Cucumis africanus Lindley f., C. anguria L. var. anguria, C. anguria var. longipes Meeuse, C. dipsaceus Ehrenb. ex Spach, C. metuliferus E. Mey ex Schrad., C. myriocarpus Naud., C. zeyheri Sond. (2x, 4x), and C. melo L. were surveyed using horizontal starch gel electrophoresis to characterize interspecific and intra-specific variation and to furnish preliminary phylogenetic information with regard to the cross-compatible group of wild African Cucumis species. Eight enzyme systems were studied: shikimate dehydrogenase, triose-phosphate isomerase, glutamic-pyruvic transaminase, glucose-phosphate isomerase, phosphogluconate dehydrogenase, isocitrate dehydrogenase, peptidase with phenylalanyl-L-proline, and phosphoglucomutase. All enzymes were polymorphic. Although C. anguria var. anguria accessions from Brazil and Ethiopia were similar, zymograms of C. anguria var. longipes were more similar to the C. melo collections. Results from var. anguria and var. longipes support previous data with regard to their varietal nature. The relative similarity among patterns of C. anguria var. longipes, C. metuliferus, and C. myriocarpus suggests a closer phylogenetic relationship than had previously been proposed. Isozyme similarities between C. anguria var. anguria, C. africanus, and C. dipsaceus suggest some phylogenetic affinities.

scholarly journals Haemoglobin polymorphism in selected farm animals: A review

2014 ◽  
Vol 30 (3) ◽  
pp. 377-390 ◽  
Author(s):  
S.S.A. Egena ◽  
R.O. Alao
Keyword(s):  
Genetic Improvement ◽  
Raw Materials ◽  
Farm Animals ◽  
Starch Gel Electrophoresis ◽  
Gene Frequencies ◽  
Starch Gel ◽  
And Performance ◽  
Polypeptide Chains ◽  
Degree Of Similarity

Biochemical diversity or polymorphism is the occurrence of varieties attributed to biochemical differences which are under genetic control. It has created a leeway for the genetic improvement of farm animals. This is because it can be used as a useful tool for the characterization of livestock breeds and population. This way, the degree of similarity or differences within and between breeds can be ascertained and this differences or similarity are important raw materials for genetic improvement of animals. Data obtained on gene frequencies and genotypes through polymorphism study makes it not only possible to compare the gene stocks of animals, the possible effects of the genes on reproductive and performance traits, but also study genetic variability under different environmental conditions of selection. This study was carried out to review haemoglobin (Hb) polymorphism in selected farm animals with the view of finding out the type of polymorphism observed by starch gel electrophoresis due to variation in the amino acid sequence in the polypeptide chains of Hb. The review showed clearly that there is a gene-controlled diversity in the different farm animals considered. This could serve as a reference point for future studies earmarked for the improvement of the animals possibly via marker-assisted selection.

Isolation and characterization of Schizosaccharomyces pombe mutants with defective NAD-dependent malic enzyme

1986 ◽  
Vol 32 (6) ◽  
pp. 481-486 ◽  
Author(s):  
C. Osothsilp ◽  
R. E. Subden
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Malic Enzyme ◽  
Pool Analysis ◽  
Starch Gel Electrophoresis ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Starch Gel ◽  
Colony Color ◽  
Color Indicator

To obtain NAD-dependent malic enzyme mutants of Schizosaccharomyces pombe, a colony color indicator screening system was developed. Mutants defective for malic acid utilization (mau mutants) are yellow, while wild-type colonies are blue on the defined bromcresol green based indicator medium. NAD-dependent malic enzyme mutants were distinguished from other mau mutants by subsequent, starch gel electrophoresis, spectrophotometry, complementation tests, and intermediate pool analysis with cell-free extracts.

scholarly journals The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 94-99 ◽  
Author(s):  
S.D. Stoneberg Holt ◽  
L. Horová ◽  
P. Bureš ◽  
J. Janeček ◽  
V. Černoch
Keyword(s):  
Poa Pratensis ◽  
Intergenic Spacer ◽  
Plastid Dna ◽  
Kentucky Bluegrass ◽  
Variety Identification ◽  
Trnl Intron ◽  
Major Band ◽  
F Region ◽  
Pcr Rflp

The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only for mature plants, but also for seedlings (less than 12 weeks old), which are difficult to distinguish morphologically. The method is quick and sensitive. When restricted with a combination of BsaJ I and Bsm I, cultivar Slezanka has one major band, Moravanka has two, and Harmonie has three. When restricted with Alu I, the heaviest band migrates most slowly for Slezanka. It is expected that many Kentucky bluegrass cultivars will share the same trnL-F sequence, so these characters alone are not sufficient for variety identification.

Biochemical characterization of Tunisian grapevine varieties

OENO One ◽  
1998 ◽  
Vol 32 (1) ◽  
pp. 17
Author(s):  
Ferjani Ben Abdallah ◽  
Farhat Chibani ◽  
Asma Fnayou ◽  
Abdelwahed Ghorbel ◽  
Jean-Michel Boursiquot
Keyword(s):  
Biochemical Characterization ◽  
Biochemical Study ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Enzyme Systems ◽  
Mother Plants ◽  
Starch Gel ◽  
Biochemical Identification ◽  
Berry Colour

<p style="text-align: justify;">61 tunisian autochton grapevine varieties have been collected for biochemical identification. Isozymes analysis with starch gel electrophoresis technique was used to confirn or to cancel random denominations awarded to the majority of these local varieties. In our conditions, concentrated plant extracts were obtained from vigorous donnant canes newly cut off from selected mother plants during automn. These allowed us to dispose of rigorously interpretable isozyme banding patterns of GPI and PGM systems and to overcome difficulties often related to the use of PGM system. The study of GPII and PGM enzyme systems allowed us to classify the autochton accessions into 16 different groups from which 5 groups containing only 2 or 3 varieties.</p><p style="text-align: justify;">On the other hand, the study of AAT and peroxydase enzyme systems has shown stable and legible isozyme banding patterns allowing to discriminate between equivalent accessions such as Sakasly and Kahli (two black local vines very similar), 3 varieties of Bidh Hamem (Bidh Hamem, Bidh Hamem Rafraf and Bidh Hamem Sfax), and 2 varieties of Bezzoul Kelba Bidha (Sfax and Gabes). In addition, certain varieties having for longtime the same denominations were characterized. A case of point the 4 varieties Khalt meaning mixture (Bouchemma, Abiedh, Mdaouer and Souche 1) and the 3 varieties of Arich (Ahmar, Dressée, and Jerba) were proved to be completely different from each other. In the same way, Bezzoul Khadem has been differed from Hemri variety. The complementary use of berry colour allowed to discriminate between Saouadi, Khdhiri and Jebbi varieties and to subdivise the remainig groups into sub-groups.</p><p style="text-align: justify;">The study of GPI, PGM, AAT and peroxydase isozyme banding patterns in combination with berry colour has led to establish a classification of the 61 autochton varieties into 37 groups including 26 varieties definitely differentiated through the results of this biochemical study.</p>

The Separation of Insect Haemolymph Proteins

1964 ◽  
Vol 96 (1-2) ◽  
pp. 110-110
Author(s):  
B. G. Loughton ◽  
P. Rueffel ◽  
H. Stich ◽  
A. S. West
Keyword(s):  
Amino Acid ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Homologous Proteins ◽  
Agar Gel ◽  
Diffusion Technique ◽  
Starch Gel ◽  
Rapid Characterization ◽  
Gel Diffusion

It has been suggested that information on the phylogenetic relationships of genera and species could be obtained by comparing the amino acid sequence in the homologous proteins of different species. This procedure is extremely difficult and time-consuming.However, a relatively rapid characterization of proteins can be obtained by analysing their mobilities with starch-gel electrophoresis and examination of antigenic diversity by the agar gel diffusion technique of Ouchterlony.

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