scholarly journals The trnL-F plastid DNA characters of three Poa pratensis (Kentucky bluegrass) varieties

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 94-99 ◽  
Author(s):  
S.D. Stoneberg Holt ◽  
L. Horová ◽  
P. Bureš ◽  
J. Janeček ◽  
V. Černoch
Keyword(s):  
Poa Pratensis ◽  
Intergenic Spacer ◽  
Plastid Dna ◽  
Kentucky Bluegrass ◽  
Variety Identification ◽  
Trnl Intron ◽  
Major Band ◽  
F Region ◽  
Pcr Rflp

The characterization of crop cultivars (varieties) will come to depend increasingly on molecular characters in addition to traditional morphological and agronomic characters. Three cultivars of Kentucky bluegrass (Poa pratensis L.), developed by the Plant Breeding Station Hladké Životice (PBHŽ), were characterized using sequences and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) patterns from the non-coding plastid trnL-F region (trnL intron, 549 bp, and trnL-trnF intergenic spacer [IGS], 344 to 364 bp). These characters could be readily and repeatably determined not only for mature plants, but also for seedlings (less than 12 weeks old), which are difficult to distinguish morphologically. The method is quick and sensitive. When restricted with a combination of BsaJ I and Bsm I, cultivar Slezanka has one major band, Moravanka has two, and Harmonie has three. When restricted with Alu I, the heaviest band migrates most slowly for Slezanka. It is expected that many Kentucky bluegrass cultivars will share the same trnL-F sequence, so these characters alone are not sufficient for variety identification.

Characterization of Fusarium spp. isolates by PCR-RFLP analysis of the intergenic spacer region of the rRNA gene (rDNA)

2006 ◽  
Vol 106 (3) ◽  
pp. 297-306 ◽  
Author(s):  
A. Llorens ◽  
M.J. Hinojo ◽  
R. Mateo ◽  
M.T. González-Jaén ◽  
F.M. Valle-Algarra ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Rflp Analysis ◽  
Rrna Gene ◽  
Fusarium Spp ◽  
Intergenic Spacer Region ◽  
Pcr Rflp

scholarly journals Introduction pages

2018 ◽  
Vol 47 (2) ◽  
pp. I-VIII
Author(s):  
Radu E. SESTRAS
Keyword(s):  
Cucumis Sativus ◽  
Poa Pratensis ◽  
Plant Root ◽  
Functional Characterization ◽  
Kentucky Bluegrass ◽  
Weed Species ◽  
Temporary Immersion System ◽  
Cucumis Sativus L ◽  
Avocado Fruit

Notulae Botanicae Horti Agrobotanici Cluj-Napoca (NBHA), Issue 2, Volume 47, 2019: The papers published in this issue Vol 47 No 2 (2019) represent new exciting researches in different topics of life science, respectively in plant science, horticulture, agronomy and crop science. Among the interesting articles we invite you to find news about temporary immersion system for micropropagation of tree species and plant root hair growth in response to hormones (reviews); sexual and vegetative propagation of Hypericum empetrifolium Willd. subsp. empetrifolium; cloning and functional characterization of CsUGD2 in cucumber (Cucumis sativus L.); potential transference of CP4 EPSPS to weed species from genetically modified Gossypium hirsutum in Northern Mexico; isolation and functional characterization of an AGAMOUS-LIKE 18 (AGL18) MADS-box gene from cucumber (Cucumis sativus L.); avocado fruit pulp transcriptomes in the after-ripening process; transcriptome sequencing of two Kentucky bluegrass (Poa pratensis L.) genotypes in response to heat stress etc. Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal has moved to online-only publication at the start of 2017; beginning in 2019, the journal appears quarterly. At the same time, we maintain our standard publication, as printed form, with ‘classic’ style - volume, issue, pagination.

scholarly journals Morphological, Pathogenic, and Molecular Characterization of Alternaria Isolates Associated with Alternaria Late Blight of Pistachio

2002 ◽  
Vol 92 (4) ◽  
pp. 406-416 ◽  
Author(s):  
Barry M. Pryor ◽  
Themis J. Michailides
Keyword(s):  
Sequence Data ◽  
Morphological Characteristics ◽  
Intergenic Spacer ◽  
Rflp Analysis ◽  
Species Group ◽  
Molecular Characteristics ◽  
Pcr Rflp ◽  
Species Groups ◽  
Selection Of

Alternaria isolates were obtained from various pistachio tissues collected in five orchards in California. For all isolates, morphological characteristics of the colony and sporulation apparatus were determined and compared with those of representative isolates of A. alternata, A. tenuissima, A. arborescens, and A. infectoria. A selection of the pistachio isolates and the representative Alternaria isolates were evaluated for pathogenicity to pistachio. Molecular characteristics of these isolates were determined using random amplified polymorphism DNA (RAPD) analysis, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis of nuclear intergenic spacer rDNA, and sequence analysis of nuclear internal transcribed spacer (ITS) rDNA. Based on morphological characteristics, the pistachio isolates were grouped as identical or very similar to either A. alternata, A. tenuissima, A. arborescens, or A. infectoria. Isolates from the alternata, tenuissima, and arborescens species-groups were pathogenic to pistachio and no significant differences in pathogenicity were observed. Isolates from the infectoria species-group were only weakly pathogenic to pistachio. Based on cluster analysis of RAPD and PCR-RFLP data, three distinct clusters were evident; the infectoria cluster, the arborescens cluster, and a combined alternata/tenuissima cluster. Based on analysis of ITS sequence data, the infectoria species-group was phylogenetically distinct from the other species-groups. Isolates of the alternata, tenuissima, and arborescens species-groups comprised a monophyletic clade in which the three species-groups could not be further resolved.

scholarly journals Random Amplified Polymorphic DNA Analysis of Dry Turfgrass Seed

HortScience ◽  
1996 ◽  
Vol 31 (3) ◽  
pp. 400-401 ◽  
Author(s):  
Patricia Sweeney ◽  
Robert Golembiewski ◽  
Karl Danneberger
Keyword(s):  
Festuca Arundinacea ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Poa Pratensis ◽  
Random Amplified Polymorphic Dna ◽  
Leaf Tissue ◽  
Creeping Bentgrass ◽  
Kentucky Bluegrass ◽  
Variety Identification ◽  
Arbitrary Primers

Random amplified polymorphic DNA (RAPD) markers from leaf tissue extractions are effective for discrimination of turfgrass varieties. The usefulness of RAPD markers for turfgrass variety identification can be enhanced by use of seed rather than leaf tissue for DNA extraction. To determine whether DNA extracted from turfgrass seed was suitable for amplification, DNA was extracted from bulk samples and individual seeds of bermudagrass [Cynodon dactylon (L.) Pers.], chewings fescue (Festuca rubra var. commutata Gaud.), Poa annua L., Poa supina Schrad., creeping bentgrass [Agrostis stolonifera L. var. palustrus (Huds.) Farw.], Kentucky bluegrass (Poa pratensis L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.). All samples were successfully amplified using an arbitrary primer. Amplification intensity varied among species. With an almost infinite number of arbitrary primers available, it is likely that suitable primers can be found to amplify DNA from most turfgrass species. Amplification of turfgrass seed DNA, whether bulk or individual seed, is possible and should prove more useful than amplification of leaf tissue DNA for discrimination of turfgrass varieties.

GENETIC CHARACTERIZATION OF STRAINS OF CYANOBACTERIA USING PCR-RFLP OF THE cpcBA INTERGENIC SPACER AND FLANKING REGIONS1

1996 ◽  
Vol 32 (3) ◽  
pp. 445-451 ◽  
Author(s):  
Christopher J. S. Bolch ◽  
Susan I. Blackburn ◽  
Brett A. Neilan ◽  
Peter M. Grewe
Keyword(s):  
Genetic Characterization ◽  
Intergenic Spacer ◽  
Pcr Rflp

Molecular Characterization of Aflatoxin Biosynthesis Genes of Aspergillus flavus from Peanuts Production Area

2019 ◽  
Author(s):  
I. Lavkor
Keyword(s):  
Fragment Length Polymorphism ◽  
Intergenic Spacer ◽  
Aflatoxin Contamination ◽  
Aflatoxin Biosynthesis ◽  
Fungal Isolates ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Production Area ◽  
Polymerase Chain

In this study, molecular analysis of (100%) all fungal isolates, which were sampled from soil and air besides from infected peanut plants in the peanut planting area, were identified in â-tubulin gene by Polymerase Chain Reaction (PCR). PCR products of fungal isolates were restricted by BglII enzyme within Restriction Fragment Length Polymorphism (RFLP). The intergenic spacer (IGS) region for aflatoxin biosynthesis genes (aflJ-aflR) were determined in 254 (78.2%) A. flavus isolates using PCR-RFLP. Selected 100 isolates were detected as A. flavus by â-tubulin sequence gene fragments and comparisons of sequence showed 96–100% similarity. 254 out of 325 isolates contained aflatoxin biosynthesis genes (aflJ-aflR), whereas 213 out of 254 isolates produced aflatoxin. The results acquired in study remarked that A. flavus was the species responsible for aflatoxin contamination. Aflatoxin gene cluster in populations can be advantage for comprehension of the toxicological risk as well as the election of biocontrol isolates.

Isolation and Characterization of a cDNA Clone Encoding an IgE-Binding Protein from Kentucky Bluegrass (Poa pratensis) Pollen

1990 ◽  
Vol 91 (4) ◽  
pp. 362-368 ◽  
Author(s):  
Shyam S. Mohapatra ◽  
Robert Hill ◽  
Jim Astwood ◽  
Abul K.M. Ekramoddoullah ◽  
Egil Olsen ◽  
...  
Keyword(s):  
Cdna Clone ◽  
Poa Pratensis ◽  
Binding Protein ◽  
Kentucky Bluegrass ◽  
Isolation And Characterization ◽  
Ige Binding

ISOZYME CHARACTERIZATION OF KENTUCKY BLUEGRASS CULTIVARS

1985 ◽  
Vol 65 (4) ◽  
pp. 985-994 ◽  
Author(s):  
N. F. WEEDEN ◽  
A. C. EMMO
Keyword(s):  
Cultivar Identification ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Polymorphism Analysis ◽  
Starch Gel Electrophoresis ◽  
Phosphogluconate Dehydrogenase ◽  
Glucose Phosphate ◽  
Starch Gel ◽  
Esterase Phenotypes

Twenty-two cultivars of Kentucky bluegrass (Poa pratensis L.) were examined for their glucose phosphate isomerase, triose phosphate isomerase, phosphoglucomutase, malate dehydrogenase, 6-phosphogluconate dehydrogenase and alpha-naphthyl esterase phenotypes. Polymorphism was observed in each isozyme system, and the first four systems were characterized for variation among and within the cultivars. Sixteen of the 22 cultivars could be uniquely identified by selecting isozymes which displayed clearly resolved bands and exhibited little or no intracultivar polymorphism. Analysis could be performed on single plants, permitting estimation of percent contamination should seed from different cultivars be mixed. Thus horizontal starch gel electrophoresis in combination with specific isozyme assays appears to offer a simple system for cultivar identification of Kentucky bluegrass seed.Key words: Cultivar identification, electrophoresis, isozymes, bluegrass (Kentucky)

scholarly journals Comparison and Characterization of Oxidation Resistance and Carbohydrate Content in Cd-Tolerant and -Sensitive Kentucky Bluegrass under Cd Stress

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2358
Author(s):  
Yong Wang ◽  
Ting Cui ◽  
Kuiju Niu ◽  
Huiling Ma
Keyword(s):  
Poa Pratensis ◽  
Soluble Sugar ◽  
Antioxidant Response ◽  
Kentucky Bluegrass ◽  
Grass Species ◽  
Cd Stress ◽  
Cd Accumulation ◽  
Structural Carbohydrate ◽  
Carbohydrate Contents

Kentucky bluegrass (Poa pratensis L.), a turf grass species that is hypertolerant of cadmium (Cd), is a potential phytoremediation material for soil polluted with Cd. However, the mechanism of Cd phytotoxicity in Kentucky bluegrass is unclear. Here, we compared the phenotype, induction of oxidative stress, and structural and non-structural carbohydrate contents between a Cd-tolerant genotype (‘Midnight’, M) and Cd-sensitive genotype (‘Rugby’, R). The results showed that both genotypes accumulated more Cd in the roots, whereas the R genotype distributed more Cd into the leaves compared with the M genotype. In both genotypes, Cd inhibited the length and fresh weight of the leaves and roots; increased the peroxidase (POD) activity but inhibited ascorbate peroxidase (APX) and catalase (CAT) activity; and increased the superoxide radical (O2−), hydrogen peroxide (H2O2), and malondialdehyde (MDA) contents. However, the M genotype exhibited lower root length inhibition, and the H2O2 and MDA contents confirmed that the M genotype had increased Cd accumulation and resistance, while the R genotype exhibited a better distribution of Cd. Moreover, Cd stress significantly increased the soluble sugar, trehalose, and sucrose contents of both genotypes. Pectin, lignin, and cellulose were significantly increased to prevent the entry of Cd into the roots. The Cd-induced growth inhibition and physiological responses in Kentucky bluegrass were preliminarily explored herein, with the chelation of pectin, lignification, and antioxidant response being possible contributors to Cd detoxification in Kentucky bluegrass. In addition, the Cd-induced increase in trehalose, sucrose, and soluble sugar contents might play a pivotal role in the defense against Cd stress in Kentucky bluegrass.

Sign in / Sign up

Export Citation Format

Share Document