scholarly journals Inheritance of spot blotch resistance in barley (Hordeum vulgare L.)

2014 ◽  
Vol 94 (7) ◽  
pp. 1203-1209 ◽  
Author(s):  
Sarvjeet Singh ◽  
Harvinder Singh ◽  
Achla Sharma ◽  
Madhu Meeta ◽  
Baljit Singh ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Recombinant Inbred Lines ◽  
Bipolaris Sorokiniana ◽  
Inhibitory Effect ◽  
Inbred Lines ◽  
Spot Blotch ◽  
Commercial Production ◽  
Hordeum Vulgare L ◽  
Heterozygous Condition ◽  
Transgressive Segregants

Singh, S., Singh, H., Sharma, A., Meeta, M., Singh, B., Joshi, N., Grover, P., Al-Yassin, A. and Kumar, S. 2014. Inheritance of spot blotch resistance in barley (Hordeum vulgare L.). Can. J. Plant Sci. 94: 1203–1209. Spot blotch, caused by Bipolaris sorokiniana, is a major disease of barley hampering its commercial production in many parts of the world. Growing resistant cultivars is the most effective, economical and eco-friendly approach to control the disease. To understand the inheritance of resistance, F1, F2 and F3 generations of two crosses, involving susceptible (PL426 and RD2503) and resistant (BL9 and BL10) parents were studied. The susceptibility of F1 plants and a ratio of 13 susceptible:3 resistant in F2 populations in both crosses indicated that the reaction to spot blotch is hypostatic and is governed by two genes with an epistatic/inhibitory effect of first on the second one. The resistant reaction appeared due to the presence of dominant allele of the second gene. The first gene in dominant homozygous or heterozygous condition had an inhibitory effect over the second gene. The inheritance pattern was confirmed from the segregation pattern of F3 progenies of both the crosses. One hundred-fifty F2:6 recombinant inbred lines (RIL) derived from PL426/BL10 cross gave a good fit to the ratio of 1 resistant:3 susceptible lines under artificial and natural epiphytotic conditions. Recovery of transgressive segregants in the RIL population indicated the presence of some modifiers or minor genes. Test of allelism involving susceptible (PL426)×susceptible (RD2503) and resistant (BL9)×resistant (BL10) parents revealed that both the susceptible parents as well as resistant parents were allelic to each other.

AAC Vitality barley

2016 ◽  
Vol 96 (3) ◽  
pp. 367-370
Author(s):  
Thin Meiw Choo ◽  
Allen G. Xue ◽  
Richard A. Martin
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Spot Blotch ◽  
Research Centre ◽  
Seed Color ◽  
Net Blotch ◽  
Good Resistance ◽  
Hordeum Vulgare L ◽  
Moderately Resistant

AAC Vitality is a six-row spring feed barley (Hordeum vulgare L.) cultivar developed by the Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada. AAC Vitality had high grain yield, good resistance to lodging, and good resistance to straw break. It was late in heading and maturity. Its seed color was bright. AAC Vitality was moderately resistant to net blotch and spot blotch. AAC Vitality performs well in Ontario.

scholarly journals Molecular Mapping of Loci Conferring Susceptibility to Spot Blotch and Resistance to Powdery Mildew in Barley Using the Sequencing-Based Genotyping Approach

2020 ◽  
Vol 110 (2) ◽  
pp. 440-446 ◽  
Author(s):  
Yueqiang Leng ◽  
Mingxia Zhao ◽  
Jason Fiedler ◽  
Antonín Dreiseitl ◽  
Shiaoman Chao ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Molecular Mapping ◽  
Recombinant Inbred Lines ◽  
Major Gene ◽  
Bipolaris Sorokiniana ◽  
Spot Blotch ◽  
Blumeria Graminis ◽  
Seedling Stage ◽  
Barley Cultivars ◽  
Trait Locus

Spot blotch (SB) caused by Bipolaris sorokiniana and powdery mildew (PM) caused by Blumeria graminis f. sp. hordei are two important diseases of barley. To map genetic loci controlling susceptibility and resistance to these diseases, a mapping population consisting of 138 recombinant inbred lines (RILs) was developed from the cross between Bowman and ND5883. A genetic map was constructed for the population with 852 unique single nucleotide polymorphism markers generated by sequencing-based genotyping. Bowman and ND5883 showed distinct infection responses at the seedling stage to two isolates (ND90Pr and ND85F) of Bipolaris sorokiniana and one isolate (Race I) of Blumeria graminis f. sp. hordei. Genetic analysis of the RILs revealed that one major gene (Scs6) controls susceptibility to Bipolaris sorokiniana isolate ND90Pr, and another major gene (Mla8) confers resistance to Blumeria graminis f. sp. hordei isolate Race I, respectively. Scs6 was mapped on chromosome 1H of Bowman, as previously reported. Mla8 was also mapped to the short arm of 1H, which was tightly linked but not allelic to the Rcs6/Scs6 locus. Quantitative trait locus (QTL) analysis identified two QTLs, QSbs-1H-P1 and QSbs-7H-P1, responsible for susceptibility to spot blotch caused by Bipolaris sorokiniana isolate ND85F in ND5883, which are located on chromosome 1H and 7H, respectively. QSbs-7H-P1 was mapped to the same region as Rcs5, whereas QSbs-1H-P1 may represent a novel allele conferring seedling stage susceptibility to isolate ND85F. Identification and molecular mapping of the loci for SB susceptibility and PM resistance will facilitate development of barley cultivars with resistance to the diseases.

Construction of a genetic map of barley (Hordeum vulgare L.) cross 'Azumamugi' × 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 284-292 ◽  
Author(s):  
Y Mano ◽  
S Kawasaki ◽  
F Takaiwa ◽  
T Komatsuda
Keyword(s):  
Hordeum Vulgare ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Recombinant Inbred ◽  
Fragment Length Polymorphism ◽  
Recombinant Inbred Lines ◽  
Amplified Fragment Length Polymorphism ◽  
Buffer System ◽  
Hordeum Vulgare L ◽  
Sequence Tagged Sites

We have devised a simple and efficient amplified fragment-length polymorphism (AFLP) system consisting of small slab gels, a discontinuous buffer system, and silver staining. Using this system, a single worker developed a barley map with 227 polymorphic fragments in 2 months. As a mapping population, 99 recombinant inbred lines of barley cultivars 'Azumamugi' × 'Kanto Nakate Gold' were used. Most of the 227 AFLP fragments showed a Mendelian segregation ratio of 1:1, and all were assigned to the seven barley chromosomes. Thus, these fragments are useful as molecular markers. They were integrated with 40 previously characterized sequence-tagged sites, 3 isozymes, and 2 morphological markers to construct an integrated map. The resulting map covered 925.6 cM with 272 markers (detecting 150 loci) at an average interval of 6.5 cM/locus. This system greatly simplifies map construction.Key words: amplified fragment length polymorphism (AFLP), Hordeum vulgare, linkage map, recombinant inbred line (RIL), sequence-tagged site (STS).

NEW TYPES OF VIRULENCE IN PYRENOPHORA TERES IN CANADA

1974 ◽  
Vol 54 (4) ◽  
pp. 731-734 ◽  
Author(s):  
A. TEKAUZ ◽  
J. T. MILLS
Keyword(s):  
Hordeum Vulgare ◽  
Spot Blotch ◽  
Cochliobolus Sativus ◽  
Pyrenophora Teres ◽  
Hordeum Vulgare L ◽  
Moderately Resistant ◽  
Hybrid Lines ◽  
Spot Type

Isolates of Pyrenophora teres (Died.) Drechsl. causing either net- or spot-type symptoms on barley (Hordeum vulgare L.) were collected in 1973 from fields near Winnipeg, Manitoba. Spot-type symptoms were similar to those of spot blotch incited by Cochliobolus sativus (Ito and Kurib.) Drechsl. ex Dastur. The cultivars Herta and Fergus, previously reported to be moderately resistant, were susceptible to both types of isolates. These two cultivars presently account for virtually all of the two-rowed barley and about half of the total barley acreage in Manitoba. The resistant hybrid lines CI 5791 and BT 201 retained their resistance to the isolates which produced net-type symptoms but were less resistant to those producing the spot type.

scholarly journals Variability of Hordeum vulgare L. Cultivars in Yield, Antioxidant Potential, and Cholinesterase Inhibitory Activity

2020 ◽  
Vol 12 (5) ◽  
pp. 1938 ◽  
Author(s):  
Joanna Kobus-Cisowska ◽  
Piotr Szulc ◽  
Oskar Szczepaniak ◽  
Marcin Dziedziński ◽  
Daria Szymanowska ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Antioxidant Potential ◽  
Aqueous Extracts ◽  
Hordeum Vulgare L ◽  
Barley Cultivars ◽  
Cholinesterase Inhibitory Activity ◽  
Barley Leaves ◽  
Barley Grains

Barley (Hordeum vulgare L.) is an annual plant cultivated in spring or autumn. Currently, over 70% of the cultivated barley grains are utilized for preparing fodder, while the rest is used for the production of malt and cereals in the food industry. The purpose of the present work was to evaluate the content of bioactive compounds, antioxidant potential, and cholinesterase inhibitory effect of the aqueous extracts of juvenile barley leaves. It was found that the barley cultivars differed in their content of the determined phytochemicals as well as their antioxidant potential and cholinesterase-inhibitory activity. The water extracts of young barley leaves contained phenolic acids as well as quercetin, rutin, and kaempferitrin. The extracts showed a higher inhibitory effect on 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) than on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals. Based on the aqueous extracts analyzed, we found that winter cultivars were characterized by the highest iron-chelating activity. Furthermore, barley extracts showed a stronger inhibitory effect against acetylcholinesterase compared to butyrylcholinesterase. The results of the present work indicated that barley cultivars differed in their germination process. Among the tested samples, the highest cholinesterase inhibitory activity was shown by the Basic variety.

Doubled haploid production by anther culture for Australian barley breeding

1992 ◽  
Vol 43 (1) ◽  
pp. 67 ◽  
Author(s):  
DJ Luckett ◽  
RA Smithard
Keyword(s):  
Hordeum Vulgare ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Environmental Conditions ◽  
Double Haploid ◽  
Spring Barley ◽  
Inbred Lines ◽  
Minimum Level ◽  
Hordeum Vulgare L ◽  
Time Of Year

Agarose-solidified media containing maltose and glutamine were used to produce double-haploid lines, by anther culture, from Australian spring barley (Hordeum vulgare L.) genotypes. Three sets of F1 and two sets of F2 breeder's hybrids were used. Genotype differences were significant for the proportion of anthers responding, and the number of green and albino regenerants per 100 anthers plated. It was confirmed that the environmental conditions experienced by the donor plants (time of year, and field or glasshouse) had a large impact upon explant performance in culture. A minimum level of anther culture efficiency was achieved to allow a breeding program to produce all its inbred lines by this method. The procedure has the potential to save research costs, and deliver improved genotypes to the grower up to four years earlier than conventional methods.

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