Construction of a genetic map of barley (Hordeum vulgare L.) cross 'Azumamugi' × 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 284-292 ◽  
Author(s):  
Y Mano ◽  
S Kawasaki ◽  
F Takaiwa ◽  
T Komatsuda
Keyword(s):  
Hordeum Vulgare ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Recombinant Inbred ◽  
Fragment Length Polymorphism ◽  
Recombinant Inbred Lines ◽  
Amplified Fragment Length Polymorphism ◽  
Buffer System ◽  
Hordeum Vulgare L ◽  
Sequence Tagged Sites

We have devised a simple and efficient amplified fragment-length polymorphism (AFLP) system consisting of small slab gels, a discontinuous buffer system, and silver staining. Using this system, a single worker developed a barley map with 227 polymorphic fragments in 2 months. As a mapping population, 99 recombinant inbred lines of barley cultivars 'Azumamugi' × 'Kanto Nakate Gold' were used. Most of the 227 AFLP fragments showed a Mendelian segregation ratio of 1:1, and all were assigned to the seven barley chromosomes. Thus, these fragments are useful as molecular markers. They were integrated with 40 previously characterized sequence-tagged sites, 3 isozymes, and 2 morphological markers to construct an integrated map. The resulting map covered 925.6 cM with 272 markers (detecting 150 loci) at an average interval of 6.5 cM/locus. This system greatly simplifies map construction.Key words: amplified fragment length polymorphism (AFLP), Hordeum vulgare, linkage map, recombinant inbred line (RIL), sequence-tagged site (STS).

A genetic map of cucumber composed of RAPDs, RFLPs, AFLPs, and loci conditioning resistance to papaya ringspot and zucchini yellow mosaic viruses

Genome ◽  
2000 ◽  
Vol 43 (6) ◽  
pp. 1003-1010 ◽  
Author(s):  
Young Hoon Park ◽  
Suat Sensoy ◽  
Crispin Wye ◽  
Rudie Antonise ◽  
Johan Peleman ◽  
...  
Keyword(s):  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Recombinant Inbred Lines ◽  
Zucchini Yellow Mosaic Virus ◽  
Amplified Fragment Length Polymorphism ◽  
Yellow Mosaic Virus ◽  
Cucumis Sativus L ◽  
Restriction Fragment Length

The watermelon strain of papaya ringspot virus (PRSV-W) and zucchini yellow mosaic virus (ZYMV) are potyviruses that cause significant disease losses in cucumber. Resistances have been identified primarily in exotic germplasm that require transfer to elite cultivated backgrounds. To select more efficiently for virus resistances, we identified molecular markers tightly linked to PRSV-W and ZYMV resistances in cucumber. We generated F6 recombinant inbred lines (RILs) from a cross between Cucumis sativus L. 'Straight 8' and a line from 'Taichung Mou Gua', TMG1 (susceptible and resistant, respectively, to both viruses), and studied the segregations of amplified fragment length polymorphism (AFLP) markers, randomly amplified polymorphic DNAs (RAPDs), restriction fragment length polymorphisms (RFLPs), and resistances to PRSV-W and ZYMV. A 353-point map of cucumber was generated, delineating 12 linkage groups at LOD 3.5. Linkage arrangements among RFLPs were consistent with previously published maps; however linkages among RAPDs in our map did not agree with a previously published map. Resistances to PRSV-W and ZYMV were tightly linked (2.2 cM) and mapped to the end of one linkage group. One AFLP cosegregated with resistance to ZYMV.Key words: amplified fragment length polymorphism, randomly amplified polymorphic DNA, restriction fragment length polymorphism, virus resistance.

Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare)

Genome ◽  
1991 ◽  
Vol 34 (3) ◽  
pp. 437-447 ◽  
Author(s):  
M. Heun ◽  
A. E. Kennedy ◽  
J. A. Anderson ◽  
N. L. V. Lapitan ◽  
M. E. Sorrells ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Cdna Clones ◽  
Hordeum Vulgare L ◽  
Chinese Spring Wheat ◽  
Restriction Fragment Length

A restriction fragment length polymorphism (RFLP) map for barley (Hordeum vulgare L.) has been developed on the basis of a population of anther culture derived, doubled haploids from the F1 of cultivars 'Proctor' × 'Nudinka'. Wheat genomic, oat cDNA, and barley cDNA clones provided the major source of the probes. Additional clones included genomic clones from Aegilops squarrosa L. and barley, two dehydrin genes, a barley promoter, and a wheat ribosomal gene. In total, 155 RFLPs and two known genes (hulless and Mla12 powdery mildew resistance) have been placed on the linkage map. Clones were assigned to chromosomes using disomic wheat–barley chromosome addition lines. Chromosome arm orientation was done using 'Chinese Spring' wheat nullisomic–tetrasomic and ditelosomic aneuploid stocks. Markers have been located on all chromosome arms except 6S, and the total map length is 1096 centimorgans (cM). Considerations involved in RFLP mapping, including choice and number of restriction endonucleases, disturbed segregation ratios, and uneven distribution of markers in the genome are discussed as well as future improvements and applications of this map.Key words: restriction fragment length polymorphism, mapping, Hordeum vulgare L., Mla12.

QTL analysis of morphological traits in a tomato recombinant inbred line population

Genome ◽  
1997 ◽  
Vol 40 (2) ◽  
pp. 242-248 ◽  
Author(s):  
I. Paran ◽  
I. Goldman ◽  
D. Zamir
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Quantitative Trait ◽  
Recombinant Inbred ◽  
Morphological Traits ◽  
Fragment Length Polymorphism ◽  
Recombinant Inbred Lines ◽  
Restriction Fragment Length

Quantitative trait loci (QTLs) influencing morphological traits were identified by restriction fragment length polymorphism (RFLP) analysis in a population of recombinant inbred lines (RILs) derived from a cross of the cultivated tomato Lycopersicon esculentum with a related wild species, Lycopersicon cheesmanii. One hundred and thirty-two RFLP loci spaced throughout the tomato genome were used as DNA probes on genomic DNA from 97 RIL families. Morphological traits, including plant height, plant fresh mass, number of branches, number of nodes, first flower-bearing node, and leaf length, were evaluated in two controlled environment trials in 1992 and 1993. QTLs were detected via regression analyses at multiple marker loci for each morphological trait. A total of 41 markers were significantly associated with the traits examined. Large additive effects were measured at many of these loci. QTLs for multiple traits were detected on chromosomes 3 (TG74) and 4 (CT188), suggesting the possible association of these chromosome segments with genes controlling growth and development in tomato. These chromosomal regions were also associated with multiple morphological traits in a L. esculentum × Lycopersicon pennellii cross. A total of 13% of the QTLs identified for traits common to both studies occupied similar map positions.Key words: Lycopersicon, quantitative trait, restriction fragment length polymorphism.

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