scholarly journals The influence of adding estradiol to semen on reproductive performance of sows

1991 ◽  
Vol 71 (2) ◽  
pp. 589-591 ◽  
Author(s):  
R. N. Kirkwood ◽  
P. A. Thacker

One hundred gilts and one hundred mixed-parity sows of Yorkshire and Landrace breeding were inseminated with semen with or without an additional 10 μg estradiol-17β. Pregnancy rates of gilts receiving estradiol-supplemented and control semen were 90 and 79%, respectively (P < 0.09). The estradiol supplementation of semen did not improve the pregnancy rates or litter size in sows. Key words: Swine, artificial insemination, estradiol

1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows


1990 ◽  
Vol 70 (1) ◽  
pp. 129-133 ◽  
Author(s):  
J. R. GILES ◽  
L. H. THOMPSON ◽  
S. ARKINS ◽  
T. CAMACHO ◽  
P. A. EICHEN

In two different experiments crossbred gilts and(or) sows were used to investigate the effects of uterine exposure (prior to breeding) to nonviable semen (NS), seminal plasma (SP) or egg albumen (EA) on reproductive performance. Treatment solutions were infused via the cervix at estrus in gilts or within 2 d post-partum in sows. Females were artificially inseminated at the subsequent estrus. In the first experiment pregnancy rates for gilts infused with Beltsville Thawing Solution (BTS) as controls, NS or EA and subsequently inseminated, were 76, 85 and 54%, respectively, and were not significantly different among treatment groups. The number of total embryos (adjusted for number of corpora lutea) present at 38 d postinsemination was 9.6, 9.0 and 9.6, respectively, for BTS, ND and EA infused gilts and was not significantly different. Likewise, the number of live and dead embryos was not different among treatments. In the second experiment, the proportions of inseminated sows infused with BTS, NS or SP that subsequently farrowed were 87, 84 and 92%, respectively, and did not differ significantly among treatments. No significant differences were detected for the number of total, live or dead pigs born per litter among treatments. We conclude that uterine exposure to seminal proteins or egg albumen prior to breeding does not alter reproductive performance in gilts by 38 d of gestation. Also, the infusion of seminal proteins in sows shortly after parturition does not affect pregnancy rates or litter size at the subsequent farrowing. Key words: Reproductive efficiency, litter size, swine, artificial insemination


1997 ◽  
Vol 77 (4) ◽  
pp. 683-688 ◽  
Author(s):  
J. P. Kastelic ◽  
D. H. McCartney ◽  
W. O. Olson ◽  
A. D. Barth ◽  
R. J. Mapletoft

Four experiments were conducted to investigate the use of melengesterol acetate (MGA) and estradiol-17β (E-17β) for synchronizing estrus and improving reproductive performance in beef cattle. Synchronized pregnancy rates were higher (P < 0.05) in heifers fed MGA after AI than controls (4 of 9 versus 0 of 7, respectively), but were not different for cows (20 of 37, 54% versus 23 of 40, 58%). Estrus rate (percentage of cows detected in estrus) was higher (P < 0.05) for cows treated with E-17β + MGA compared with those treated with cloprostenol alone (85.0 versus 65.9%). Synchronized pregnancy rates were not significantly affected by feeding MGA or grain only after detected estrus and AI (20 of 31, 64% versus 17 of 30, 57%). For 20 cows not detected in estrus and bred 100 h after cloprostenol treatment, pregnancy rate was higher (P < 0.05) for those fed MGA after AI than controls (6 of 11, 54% versus 1 of 9, 11%). Feeding MGA after breeding may help to improve pregnancy rates. Key words: Melengesterol acetate, estradiol-17β, estrus, reproductive performance, beef cattle


1988 ◽  
Vol 68 (1) ◽  
pp. 69-81 ◽  
Author(s):  
MOHAMED H. FAHMY ◽  
JACQUES J. DUFOUR

Reproductive performance and body weight were studied on 361 ewes, representing Finnsheep (F), DLS (a population of 1/2 Dorset, 1/4 Leicester, 1/4 Suffolk) and seven combinations ranging from 1/8 to 7/8 Finnsheep breeding. Conception rate in yearlings was 61.5% for DLS compared to 89.0% for F with the crosses being intermediate. Conception rate in older ewes was similar in the different genetic groups (avg. 94%). Ovulation rate and litter size at birth of DLS ewes were 1.72 and 1.44 lambs, which was less than half those of F ewes (3.51 and 2.86 lambs, respectively). Both traits increased progressively with an increase in F breeding in crosses and with advances in age. DLS ewes weaned 1.22 lambs compared to 2.03 lambs for F ewes and 1.84 lambs for 4/8 F ewes. The heaviest litters at weaning (31.7 kg) were raised by 4/8 F ewes, followed by 7/8 F (30.8 kg) while those raised by DLS ewes weighed 23.0 kg and F ewes 29.1 kg. Percentage of ova lost per ewe mated averaged 24% and ranged between 18% (DLS and 1/8 F) and 29% (6/8 F). About 3.6% of lambs were born dead and a further 13.8% died before weaning. Preweaning mortality rate was highest in F (22.9%) and lowest in 3/8 F (9.4%). Average kilograms of lambs weaned per ewe exposed was highest in 4/8 F (27.6 kg) followed by F (26.0 kg), whereas that of DLS was the lowest at 18.1 kg. The 4/8 F cross showed 25% heterosis in kg of lambs weaned per ewe exposed and 52.5% increase over DLS. Significant positive linear regressions were calculated for ovulation rate, litter size and preweaning mortality rate on proportion of Finnsheep breeding in crosses. The relation was quadratic for percent ova lost and lamb mortality at weaning. Yearling DLS females weighted 36 kg compared to 44 kg for F yearlings. However, at 5 yr of age DLS ewes weighed 62 kg, 5 kg heavier than F ewes. The heaviest ewes at all ages were the 4/8 F (45 kg at 1 yr, 65 kg at 5 yr). Key words: Reproduction, DLS sheep, Finnsheep, crossbreeding, heterosis, repeatabilities


2015 ◽  
Vol 13 (2) ◽  
pp. 43-47
Author(s):  
P Myagmarsuren ◽  
U Myagmardulam ◽  
Ts Haliunaa

In all domestic species, acceptable pregnancy rate with frozen-thawed semen is 20-30% through simply cervical and vaginal insemination. Researchers have proved that achievable high results can be expected only from intrauterine insemination in ewes. Unlike the cow, and goat, the cervix of ewes is a formidable barrier to penetrate for transcervical intrauterine insemination. It is not only due to the physical size of the canal and a narrow luminal diameter (even at estrus), but also due to its caudally facing eccentric series of four to eight funnel-like rings. The major aim of this study was to evaluate a comparison of transcervical and laparoscopic intrauterine artificial insemination techniques with frozen –thawed semen on the reproductive performance of ewes.58ewes of 3-4 years of age, so called Altanbulag subspecies of breed were selected and equally divided into 2 groups for the AI methods from October 15-20, 2013 in the breeding station of Research Institute of Animal Husbandry in Hongor sum, Darhan uul province. The estrus was synchronized with EAZI-BREED TMCIDR in which the progesterone content was 0.3 g. After 12 days, the CIDR were removed, PMSG of 400 and 200 IU were administrated intramuscularly per ewe. The total success of synchronization rate was 93.3%. The results has shown the possibilities of laparoscopic and transcervical AI techniques in 55.17%, 41.37% (p>0.05) of pregnancy rates respectively, with frozen-thawed semen in ewes by ultrasonography diagnosis.Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 43-47


1979 ◽  
Vol 59 (4) ◽  
pp. 685-691 ◽  
Author(s):  
G. A. LANGFORD ◽  
G. J. MARCUS ◽  
A. J. HACKETT ◽  
L. AINSWORTH ◽  
H. F. PETERS ◽  
...  

The reproductive performance of crossbred sheep maintained in total confinement was compared after artificial insemination with fresh or frozen semen. Estrus was synchronized with progestagen-impregnated vaginal sponges and pregnant mares’ serum gonadotropin. Inseminations were performed 54 and 60 h after sponge removal. The fertility of ewes inseminated with fresh semen was significantly higher than of ewes inseminated with frozen semen. Conception rates, lambing rates and litter size were 83%, 78% and 2.2 using fresh semen and 65%, 43% and 1.8 using frozen semen. In a group of similar ewes bred by natural service, the lambing performance was comparable to that obtained with fresh semen. The difference between conception and lambing rates suggests an increase in early embryonic mortality when breeding with frozen semen and confirms the need for improved frozen semen technology.


2013 ◽  
Vol 25 (3) ◽  
pp. 581 ◽  
Author(s):  
Miguel Mellado ◽  
Claudia G. Orta ◽  
Eloy A. Lozano ◽  
Jose E. García ◽  
Francisco G. Veliz ◽  
...  

The objectives of this study were to examine the effects of several factors affecting fawning rate, litter size, litter weight and neonatal fawn mortality in white-tailed deer inseminated either transcervically or by means of laparoscopy. Oestrus synchronisation with a controlled internal drug release (CIDR)-based protocol and fixed-time artificial insemination (FTAI) was conducted in 130 white-tailed deer (Odocoileus virginianus texanus) during three reproductive seasons (2007–2009; 271 services) in a game-hunting ranch in a hot–arid environment (26°4′ N, 101°25′ W). Ninety additional non-treated does were exposed to bucks for natural mating. Fawning rate did not differ between AI methods (40.0 vs 45.0% for transcervical and laparoscopic AI, respectively). Overall fawning rate (proportion of all does fawning after FTAI and a subsequent period of buck exposure) did not differ between transcervical (89.5%), laparoscopic (80.3%) or natural (88.9%) insemination. Litter size per fawning doe was higher (P&lt;0.05) in naturally-served does (1.65±0.48) than in transcervically-inseminated does (1.40±0.51) or in laparoscopically-inseminated does (1.48±0.50). The main conclusion was that no enhancement of fawning rate or litter size occurred as a result of intrauterine deposition of semen by laparoscopy compared with the transcervical insemination technique.


2019 ◽  
Vol 47 (1) ◽  
Author(s):  
João Batista Souza Gorges ◽  
Andressa Varella Gonsioroski ◽  
Eduardo Pradebon Da Silva

Background: More recently, polyunsaturated fatty acids (PUFA) supplements have been used in grazing beef herds in attempt to influence specific metabolic pathways and hormones that directly modulate reproductive function. Supplementation of calcium salts of PUFA can positively influence ovarian follicular growth, luteal function, and improve reproductive performance in dairy and beef females. The aim of this experiment was to evaluate strategies of dietary treatments using PUFA supplementation for 30 days before or after FTAI protocol and its effects on of ovulatory follicle size, ovulation, pregnancy and gestational losses rates in beef heifers.Materials, Methods & Results: One hundred and ninety-nine Brangus heifers (24 ± 3 months-old; BW= 320 ± 15 kg; BCS= 2.85 ± 0.17) were randomly assigned in three different groups to receive dietary treatments. Group 1 (n = 70) was fed with 0.5 kg/day of a protein-mineral mixture supplement containing 0.2 kg/day PUFA (Mix A)during 30 days before FTAI, Group 2 (n = 68) received the same supplement 30 days after FTAI and Control (n = 61) only the supplement without PUFA (Mix B) during all period. Heifers were submitted to estrous synchronization treatment using progesterone (P4), estradiol benzoate (E2) and prostaglandin F2a  (PGF). Ultrasound exams were done to measure ovulatory follicle diameter and to determinate ovulation and pregnancy rates on Day 30 and 60 after FTAI. Daily gain (DG) was higher (P < 0.05) only when heifers received supplement containing PUFA (Group 1= 30 days prior FTAI) and (Group 2= 30 days after FTAI). PUFA supplementation before FTAI also resulted in larger ovulatory follicles diameter (P < 0.05) in Group 1 (11.2 ± 2.5 mm) compared to Group 2 (9.9 ± 2.3 mm) and Control (10.0 ± 2.07 mm). Ovulation rates were similar (P > 0.05) in Group 1 (90%), Group 2 (75%) and Control (67%). Pregnancy rates were higher (P < 0.05) in Group 1 (Day 30= 67.1% and Day 60= 64.2%) when compared with Group 2 (Day 30= 48.5% and Day 60= 44.1%) and Control (Day 30= 49.2% and Day 60= 45.9%). Gestation losses were similar (P > 0.05) among Groups 1 (6.6%), 2 (9%) and Control (6.6%).Discussion: Rumen-protected supplemental lipids have been used to increase energy density and to determine direct positive effects on reproduction in dairy and beef herds.  There is evidence that feeding moderate amounts of PUFA supplements can improve energy status and result in significant differences in DG as it was observed during experimental period. Thus, heifers that received 0.2 kg/day of PUFA supplement 30 days before FTAI protocol had also a positive effect on ovulatory follicles size, but ovulation rates were similar in groups. Beneficial effects of pre-breeding PUFA supplementation were observed on pregnancy rates on Days 30 and 60 after FTAI, but pregnancy loss was not different among dietary treatments in beef heifers. Based on this study, PUFA supplementation before FTAI protocol had a positive effect on reproductive performance of beef heifers by directly improving ovarian function, uterine environment and early embryonic development. Results indicate that pre-breeding PUFA supplementation to beef heifers is a suitable strategy to increase pregnancy rate in FTAI programs. Therefore, for a better understanding of interactions of nutritional strategies and utero-ovarian functions more evaluations are needed to understand the mechanisms by which feeding PUFA may improve reproductive function in beef heifers.


2009 ◽  
Vol 33 (6) ◽  
pp. 1671-1674
Author(s):  
Joana Miller ◽  
Ana Luísa Neves Alvarenga ◽  
Luis David Solis Murgas ◽  
Adriana Cristina da Silva ◽  
Ricardo Sales Araújo ◽  
...  

The aim of this work was to evaluate the efficiency of the intrauterine insemination (IUI) in swine, considering the conception rate, farrowing rate, litter size (alive born pigs). For the IUI, the females had been insemination at 24 and 48 hours after the estrus detection, and the inseminating doses of 500 million, 1 billion, 1.5 billion and 2 billion spermatozoa in 20 mL extender had been used. The procedure of catheter insertion through the cervical canal was successfully performed in 97.9% of the females. The conception rate was 6.3% in the IUI. The farrowing rate in IUI was 87.2% but the farrowing rate was 100% for the sperm concentration of 500 million. Regarding the number of born pigs and alive born pigs observed in females inseminated with IUI, no significant difference was observed (p > 0.05). The concentration of 500 x 10(6) spermatozoa in 20 mL extender in the intrauterine insemination resulted in an optimal reproductive performance.


2011 ◽  
Vol 23 (1) ◽  
pp. 146
Author(s):  
T. Okazaki ◽  
T. Akiyoshi ◽  
M. Kan ◽  
H. Teshima ◽  
M. Shimada

Epididymal spermatozoa are one of the available male germ cells for cryopreservation. It has been reported that frozen–thawed porcine epididymal spermatozoa have a high fertilization competence in vitro as compared with that in ejaculated one. However, there is little information about reproductive performance, such as conception rate or litter size, after artificial insemination (AI) using frozen–thawed epididymal spermatozoa. Recently, we demonstrated that the addition of seminal plasma to thawing solution improves membrane and acrosomal integrity, and enhanced both in vivo and in vitro fertilizing activity of frozen–thawed ejaculated spermatozoa. Moreover, the injection of seminal plasma to uterus with frozen–thawed spermatozoa significantly increased the number of implantation site (Okazaki et al. 2009 Theriogenology 71, 491–498). Thus, to apply those positive functions of seminal plasma to AI using frozen–thawed epididymal sperm, in this study, we added seminal plasma to thawing solution and then analysed the sperm functions including AI test using frozen–thawed epididymal spermatozoa. Epididymal spermatozoa collected by flushing caudal epididymis were frozen as described in our previous study (Okazaki et al. 2009). Frozen-spermatozoa were thawed in Modena solution with or without different percentages of seminal plasma. Protein tyrosine phosphorylation as a marker of capacitation was detected by western blotting. To examine the reproductive performance, the sows of natural oestrus were artificially inseminated two times (5 × 109 50 mL–1 per injection). When the frozen–thawed ejaculated or epididymal sperm was incubated up to 6 h, the motility of epididymal sperm was significantly higher than that of ejaculated sperm (19.6 v. 37.6%). However, the acrosomal membrane was damaged in epididymal sperm group at 3-h incubation period (15.2 v. 36.0%). The addition of seminal plasma [0, 10, 15, 20% (v/v)] in Modena solution protected the acrosomal injury (3 h; 35.2, 19.5, 15.6, 14.6%) and maintained high rate of motility (6 h; 38.8, 48.8, 62.5, 60.0%) in a dose-dependent manner. Furthermore, the addition of seminal plasma suppressed the expression of the 15 kDa phosphoprotein (early capacitation status), and the maximum effect was detected at 15% (v/v) seminal plasma. When the frozen–thawed epididymal spermatozoa with 15% (v/v) seminal plasma were artificially inseminated to swine (n = 15), the conception rate and the mean number of litter size were increased as compared with control (93 v. 43%, 10.0 v. 5.0). From these results, we concluded that the addition of seminal plasma to thawing solution was a beneficial method for artificial insemination using frozen–thawed epididymal spermatozoa in the pig. This work was supported by the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry, and JST-Grant (No. 12-068 and No. 12-104).


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