scholarly journals PROLINE SUPPLY FOR EFFICIENT NITROGEN UTILIZATION BY LACTATING RUMINANTS

1984 ◽  
Vol 64 (5) ◽  
pp. 285-286 ◽  
Author(s):  
I. BRUCKENTAL ◽  
EUGENIA ALUMOT

To prove the sparing action of proline on arginine utilization by the mammary gland, proline was administered to the duodenum of goats in early lactation. As found previously in mid-lactation, arginine uptake by the mammary gland dropped significantly due to proline addition. Cow mammary tissue was cultured in media with increasing concentrations of proline. The radioactivity of proline formed from [U-14C] arginine was measured in hydrolysates of casein synthesized by the gland. Increasing proline concentrations caused a corresponding decrease in proline labelling. The addition of proline, protected against rumen degradation, may save dietary protein in the ration of high-producing dairy cows. Key words: Proline, lactating ruminants

2019 ◽  
Author(s):  
◽  
Ricardo Oliveira Rodrigues

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Disruptive effects of climate change, such as increasing environmental temperature, have direct impacts on economic viability and efficiency of food production. In lactating dairy cows, heat stress reduces milk production and alters function of mammary secretory cells, at least partly by disturbing local protein metabolism. We hypothesized that hyperthermia would not only reduce mammary blood flow but would also reduce mammary extraction of nutrients from blood. In addition, we hypothesized that transcriptional profiling of mammary tissue would reveal disruption of cellular homeostasis. Our objective was to determine the effects of hyperthermia on mammary function. More specifically, we aimed to profile mammary blood flow and the changes in mammary transcriptome of heat-stressed lactating dairy cows. We investigated the effects of early and prolonged exposure of lactating dairy cows to hyperthermia by exposing cows to programmed constantly elevated temperature and humidity to induce and maintain body temperature approximately 1[degree]C above normal. Experiments were conducted to evaluate the production responses of hyperthermic lactating dairy cows, to characterize total and nutritive mammary blood flow, and to elucidate the regulation of mammary function during early and prolonged exposure to hyperthermia. Results from these studies established that 1) hyperthermia reduces total and nutritive mammary blood flow, limiting nutrient disappearance across the mammary gland; 2) hyperthermia does not induce shunting of blood away from the gland; 3) hyperthermia affects mammary tissue transcriptome, mainly altering processes associated with ECM and cell adhesion; 4) the effects of exposure to prolonged heat stress on mammary gene expression are distinct from the effects of feed restriction, in lactating dairy cows; and 5) mammary function is reestablished within 8 days after cessation of heat stress.


2001 ◽  
Vol 41 (6) ◽  
pp. 823 ◽  
Author(s):  
C. R. Stockdale

With the current increases in genetic merit and feeding occurring at farm level, dairy cows are under increasing nutritional stress in early lactation. Cows obtain their energy at this time from the feeds they eat and from body reserves. The relationship between body condition at calving and productivity of dairy cows has been reviewed, with particular emphasis on interactions between body condition and nutrition in early lactation. Recent research on the influence of body condition at calving on subsequent milk productivity, conducted mainly in the United Kingdom with complete diets fed indoors, has produced results in apparent conflict with the previous results from southern Australia and New Zealand where cows grazed pasture. In particular, the overseas research suggests considerably less advantage to improvements in body condition than had been previously thought. It is concluded that more information is needed concerning the interaction between body condition at calving and nutrition in early lactation, with dietary energy and protein both being important. There is a suggestion that, when complete diets are fed, it is better to achieve high energy concentrations in post-calving diets by the use of high-fibre concentrates with a fat supplement, rather than with high-starch concentrates. This has implications for dairying in Australia, since cereal grains are the major energy supplement used on many farms in early lactation and recent research has indicated that immediate marginal milk production responses to the use of concentrates may be poorer with fat cows than with thin cows. Reports from controlled feeding experiments indicate that fat cows need more dietary protein than thin cows and undegradable dietary protein might be of more concern than rumen degradable protein. However, in dairy systems where pasture is a considerable proportion of the diet, benefits of supplying specific undegradable dietary protein supplements still need to be established. Recent research has suggested that pasture appears to provide considerable quantities of undegradable dietary protein, even though the crude protein in pasture is potentially highly degradable in the rumen. Body condition at calving may also affect subsequent reproductive performance. This is due to its association with the degree of negative energy balance occurring in early lactation and because fat cows may be more susceptible to metabolic disease(s). While the mechanisms involved are probably quite complex, increases in animal productivity will generate more stress in cows at a time of their annual cycle when stress needs to be minimised. Further understanding is required to link the relevancy of overseas research to Australian dairy farming conditions where pasture is a key input.


Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1191
Author(s):  
Shengtao Gao ◽  
Zheng Zhou ◽  
Jiaqi Wang ◽  
Juan Loor ◽  
Massimo Bionaz ◽  
...  

The objective of the present study was to evaluate the overall adaptations of liver and mammary tissue to a corn stover (CS) compared to a mixed forage (MF) diet in mid-lactation primiparous dairy cows. Twenty-four primiparous lactating Holstein cows were randomly allocated to 2 groups receiving either an alfalfa forage diet (MF, F:C = 60:40) with Chinese wildrye, alfalfa hay and corn silage as forage source or a corn stover forage diet (CS, F:C = 40:60). A subgroup of cows (n = 5/diet) was used for analysis of liver and mammary transcriptome using a 4 × 44K Bovine Agilent microarray chip. The results of functional annotation analysis showed that in liver CS vs. MF inhibited pathways related to lipid metabolism while induced the activity of the potassium channel. In mammary tissue, fatty acid metabolism was activated in CS vs. MF. In conclusion, the analysis of genes affected by CS vs. MF indicated mammary gland responding to lower level of linoleate from the diet (lower in CS vs. MF) by activating the associated biosynthesis metabolic pathway while the liver adaptively activated potassium transport to compensate for a lower K ingestion.


Reproduction ◽  
2011 ◽  
Vol 141 (3) ◽  
pp. 373-380 ◽  
Author(s):  
Amy-Lynn Frankshun ◽  
Teh-Yuan Ho ◽  
David C Reimer ◽  
Joseph Chen ◽  
Salamia Lasano ◽  
...  

A lactocrine mechanism for delivery of maternally derived relaxin (RLX) into the neonatal circulation as a consequence of nursing was proposed for the pig. Immunoreactive RLX was detected in colostrum and in the serum of newborn pigs only if they were allowed to nurse. Milk-borne RLX concentrations are highest during early lactation (9–19 ng/ml), declining to <2 ng/ml by postnatal day 14. Whether milk-borne RLX is bioactive is unknown. Evidence that RLX concentrations in milk are higher than in maternal circulation in several species suggests the mammary gland as a site of local RLX production. It is unknown whether the porcine mammary gland is a source of RLX. Therefore, objectives were to evaluate RLX bioactivity in porcine milk during the first 2 weeks of lactation, identify the form of RLX in porcine milk, and determine whether mammary tissue from early lactation is a source of milk-borne RLX. Milk RLX bioactivity was determined using anin vitrobioassay in which cAMP production by human embryonic kidney (HEK293T) cells transfected with the human RLX receptor (RXFP1) was measured. RLX bioactivity was highest at lactation day (LD) 0, decreasing to undetectable levels by LD 4. Immunoblot analysis of milk proteins revealed an 18 kDa band, indicating proRLX as the primary form of RLX in porcine milk. ProRLX protein and transcripts were detected in porcine mammary tissue on LD 0 and 7. Results support the lactocrine hypothesis by defining the nature and a potential source for bioactive proRLX in porcine colostrum/milk.


2019 ◽  
Vol 86 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Ye Lin ◽  
He Lv ◽  
Minghui Jiang ◽  
Jinyu Zhou ◽  
Shuyuan Song ◽  
...  

AbstractIn this research communication we used digital gene expression (DGE) analysis to identify differences in gene expression in the mammary glands of dairy cows between early lactation and the mid-dry period. A total of 741 genes were identified as being differentially expressed by DGE analysis. Compared with their expression in dry cows, 214 genes were up-regulated and 527 genes were down-regulated in lactating cow mammary glands. Gene Ontology analysis showed that lactation was supported by increased gene expression related to metabolic processes and nutrient transport and was associated with decreased gene expression related to cell proliferation. Pathway mapping using the Kyoto Encyclopedia of Genes and Genomes showed that 579 differentially expressed genes had pathway annotations related to 204 pathways. Metabolic pathway-related genes were the most significantly enriched. Genes and pathways identified by the present study provide insights into molecular events that occur in the mammary gland between early lactation and mid-dry period, which can be used to facilitate further investigation of the mechanisms underlying lactation and mammary tissue remodeling in dairy cows.


1980 ◽  
Vol 63 (5) ◽  
pp. 833-837 ◽  
Author(s):  
Donald W. Claypool ◽  
M.C. Pangboan ◽  
H.P. Adams

2003 ◽  
Vol 90 (2) ◽  
pp. 271-281 ◽  
Author(s):  
D. Pacheco ◽  
M.H. Tavendale ◽  
G. W. Reynolds ◽  
T. N. Barry ◽  
J. Lee ◽  
...  

The utilisation of essential amino acids (EAA) by the mammary gland of lactating dairy cows fed fresh forages was studied to provide basic information useful in designing strategies to increase the production of milk protein from pasture-fed dairy cows. The relationship between the flux of EAA in the whole body and their uptake by the mammary gland was determined in four cows in early lactation (length of time in milk 44 (SD 14·5) d) producing 21 (SD 4·0) kg milk/d. The cows were maintained in metabolism stalls and fed fresh perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) pasturead libitumor restricted to 75 %ad libitumintake. The whole-body fluxes of amino acids (AA) were measured using an arterio-venous infusion of universally13C-labelled AA. Whole-body fluxes of fourteen AA were estimated. Isotope dilution indicated that mammary utilisation accounted for one-third of the whole-body flux of EAA, with individual AA ranging between 17 and 35 %. Isoleucine, leucine, valine and lysine were the EAA with the greatest partitioning towards the mammary gland (up to 36 % of the whole-body flux), which could reflect a potentially limiting effect on milk protein synthesis. In the case of AA with low partitioning to the mammary gland (for example, histidine), it is suggested that non-mammary tissues may have priority over the mammary gland and therefore the supply of this AA may also limit milk protein synthesis.


1995 ◽  
Vol 35 (1) ◽  
pp. 19 ◽  
Author(s):  
CR Stockdale

Eight experiments were conducted over 2 years with 92 lactating dairy cows individually fed various combinations of irrigated perennial pasture and maize silage in an indoor feeding facility. Responses to different amounts of maize silage and pasture in early and late lactation were measured. Daily pasture intake ranged from 6.2 to 12.4 kg dry matter (DM)/cow, while maize silage intake ranged from 0 to 12.4 kg DM/cow. The marginal return to feeding maize silage at up to 5 kg DM/cow.day to cows in early lactation eating about 7 kg DM/day of pasture as their basal ration was 0.89 kg extra milk for each kg DM maize silage eaten. In late lactation, this was reduced to 0.63 kg milk/kg DM. Milk yield responses to maize silage at both stages of lactation were lower at higher levels of pasture feeding. Although milk fat content was maintained over a wide range of maize silage, feeding large quantities of maize silage in early lactation was detrimental to milk production. The likely explanation for poor animal performance at the high levels of maize silage was dietary protein insufficiency.


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