NON-PROTEIN NITROGEN IN CORN SILAGE: A PARTIAL CHARACTERIZATION, ITS UTILIZATION IN THE RUMEN AND EFFECT UPON DIGESTIBILITY AND RETENTION OF NITROGEN IN LAMBS

1978 ◽  
Vol 58 (4) ◽  
pp. 681-690 ◽  
Author(s):  
J. G. BUCHANAN-SMITH ◽  
Y. T. YAO
Keyword(s):  
Soybean Meal ◽  
Nitrogen Balance ◽  
Corn Silage ◽  
Partial Characterization ◽  
Release Rates ◽  
Protein Nitrogen ◽  
Total N ◽  
Ammonia Release ◽  
Urea Infusion

Three trials were conducted. In trial 1, 30 samples of silage were collected from farms and analyzed. Non-protein nitrogen (NPN) contributed an average of 46.4% to the total N. Of the NPN-N, an average of 10.1% was ammonia-N, 30.4% was alpha amino-N, 1.2% was amide-N and 58.3% was not identified. Extensive variability was demonstrated among samples for all these values except ammonia-N. In trial 2, silage effluents, in which NPN-N was 95% or more of total N, were compared with urea and soybean meal as to ammonia release rates in the rumen after isonitrogenous infusions of each material. Ammonia levels after infusion of corn silage effluent were nearer to those after soybean meal infusion than those after urea infusion. In trial 3, frozen corn (low in NPN) was compared with ensiled corn in a digestion and balance trial using lambs. Nitrogen balance was not significantly different between corns but was improved by supplementing either of them with urea.

NITROGEN ADDITIVES TO CORN SILAGE FED TO GROWING CALVES

1976 ◽  
Vol 56 (2) ◽  
pp. 285-290 ◽  
Author(s):  
D. N. MOWAT ◽  
J. G. BUCHANAN-SMITH ◽  
G. K. MACLEOD ◽  
J. E. CORE
Keyword(s):  
Soybean Meal ◽  
Feed Efficiency ◽  
Dry Matter ◽  
Corn Silage ◽  
Dry Matter Intake ◽  
Feeding Time ◽  
Protein Nitrogen ◽  
Weight Gains ◽  
True Protein ◽  
Silage Additives

Three separate experiments were conducted to investigate performance of light-weight calves fed corn silage supplemented with different non-protein nitrogen (NPN) sources added at ensiling time. In one experiment NPN was also added at feeding time. For calves up to approximately 290 kg body weight all supplements containing only NPN reduced weight gains by approximately 0.10–0.15 kg/day compared to soybean meal or low-urea supplements. Corn silage treated with NPN silage additives resulted in reduced dry matter intake compared to soybean meal supplementation. Thus, the effect on feed efficiency was not as great as on weight gains. Treated silages tended to be higher in pH and lactic acid but lower in acetic acid content. A large portion of the NPN added at ensiling time was analyzed as ammonia in the silages. Also, true protein content of the treated silages tended to be higher than that of the controls.

EFFECT OF NITROGEN FERTILIZATION AND PLANT POPULATION ON THE VALUE OF CORN SILAGE FED TO BEEF STEERS

1984 ◽  
Vol 64 (2) ◽  
pp. 453-458 ◽  
Author(s):  
PAUL M. FLIPOT ◽  
WESLEY MASON ◽  
JEAN GENEST
Keyword(s):  
Soybean Meal ◽  
Feed Efficiency ◽  
N Fertilization ◽  
Plant Population ◽  
Corn Silage ◽  
Beef Steers ◽  
Higher Plant ◽  
Total N ◽  
Nitrogen Levels ◽  
Plant Moisture Content

Corn was grown at 86 000 plants/ha and at 129 000 plants/ha and fertilized with nitrogen at rates of 150 and 200 kg/ha in order to determine the effect of population and N-fertilization on field production, silage preservation and animal performance. Silages from the four field treatments were offered ad libitum to Hereford steers during 112 days. Within each of the corn treatments, half of the animals were supplemented with 450 g of soybean meal (48% protein). Under field conditions characteristic of this trial, the higher plant populations and higher nitrogen levels failed to increase corn silage yields and resulted in lower ear production, later maturing plants and a higher plant moisture content at harvest. The trends were most noticeable in the high plant population treatments. The quality of the silage was not affected by the different treatments. No significant differences for total N, soluble N, NH3-N, lactic acid and acetic acid were noted among silages. Animal intake was increased with silages produced on high N-fertilization at 86 000 and 129 000 plants/ha. No significant differences in animal gain or feed efficiency were observed due to field treatments. The addition of soybean meal to the silages diluted the influence of field treatment on animal intake. However, within field treatments the supplement significantly improved dry matter intake, gain and feed efficiency. This study showed no advantage in increasing corn population over 86 000 plants/ha or N fertilization above 150 kg/ha. Key words: Corn silage, plant population, N-fertilization, steers

scholarly journals Feeding strategies for improving productivity of growing steers fed grass silage

2008 ◽  
Vol 88 (4) ◽  
pp. 685-692
Author(s):  
D. R. Ouellet ◽  
L. Faucitano ◽  
D. Pellerin ◽  
M. D’Amours ◽  
R. Berthiaume
Keyword(s):  
Particle Size ◽  
Soybean Meal ◽  
Nitrogen Balance ◽  
Average Daily Gain ◽  
Latin Square ◽  
Feeding Strategies ◽  
Beef Steers ◽  
Plasma Urea ◽  
Grass Silage ◽  
Ground Corn

Two experiments were conducted to determine the relationship between corn particle size and soybean meal treatment on growth, diet digestibility, and nitrogen balance of growing steers. In the first experiment, 40 medium-frame beef steers (250 ± 11 kg) were fed individually for 140 d a diet based on grass silage offered for ad libitum consumption and supplemented with either 3.5 kg of DM d-1 of cracked corn (CC) or ground corn (GC) and with 450 g of DM d-1 of solvent extracted (SS) or lignosulfonate-treated soybean meal (Soypass™ SP). Dry matter intake was not affected by treatments and averaged 8.6 ± 0.3 kg d-1 (P > 0.10). Average daily gain was higher for animals receiving the ground corn than those fed cracked corn. Feed to gain ratio was not affected by treatments. There was an interaction between treatments for plasma urea-N concentration, with source of soybean meal having no effect with CC. When compared with SS, SP supplement reduced plasma urea-N when fed with GC. No effect of soybean meal and its interaction with corn processing was observed on growth performance. In the second experiment four additional steers were used in a 4 × 4 Latin square design to evaluate diet digestibility and nitrogen balance. There was an interaction between treatments for starch apparent digestibility and N retained, the values being respectively, 90, 86, 93, and 92% (SEM = 1.2; P < 0.01) and 36, 42, 44 and 41 g d-1 (SEM = 2.8; P < 0.03) for CCSS, CCSP, GCSS and GCSP, respectively. Altogether, the results indicate a slight advantage to reduce particle size of corn in growing steers fed grass silage. However, soybean meal treatment resulted in limited effects on growth and digestion. Key words: Rumen carbohydrate, undegradable protein, performance, steers

scholarly journals Feed quality in swine diet

2002 ◽  
Vol 56 (1-2) ◽  
pp. 63-72
Author(s):  
Branislav Zivkovic ◽  
Stanimir Kovcin ◽  
Mihal Fabjan
Keyword(s):  
Soybean Meal ◽  
Animal Origin ◽  
Negative Effects ◽  
Genetically Modified Soybean ◽  
Protein Nitrogen ◽  
Economic Production ◽  
Synthetic Amino Acids ◽  
Soybean Whey ◽  
Swine Diet

The paper will demonstrate the quality of some feed used in swine diet. The emphasis will be on feed whose incorporation into mixes could result in unfavorable effects on production, health and economic production of swine. Data will be presented on maize and its possible negative effects, having in mind toxins. Soybean meal, or genetically modified soybean meal, will also be observed. The next feed which will be discussed will be soybean whey obtained by different procedures and the potential dangers of its use in swine diet rations. Sunflower meal, feed of animal origin, with emphasis on fish flour and meat-bone flour will also be covered in the work. A feed which has been attracting particular attention lately is yeast imported from Italy. Its quality characteristics will be discussed, the so-called non-protein nitrogen. Analyses of mineral feed will include sources of phosphorus, phosphates (monocalciumphosphate, dicalcium phosphate) phytases and resolving the problem of phosphorus in swine rations. Finally, an inevitable segment are synthetic amino acids, especially lysine and its role in swine diet.

scholarly journals Effect of sample size on kinetic parameters of roughage and concentrated feeds by a semi-automated in vitro gas production system

2020 ◽  
Vol 41 (1) ◽  
pp. 255
Author(s):  
Mariane Moreno Ferro ◽  
Luciano da Silva Cabral ◽  
Livia Vieira de Barros ◽  
Claudio Vieira de Araujo ◽  
Nelcino Francisco de Paula
Keyword(s):  
Cottonseed Meal ◽  
Soybean Meal ◽  
Gas Production ◽  
Corn Silage ◽  
Distillers Grains ◽  
Napier Grass ◽  
Distillers Grains With Solubles ◽  
Grass Silage ◽  
Corn Distillers ◽  
Castor Meal

This study aimed to evaluate the effect of different amounts of incubated samples on the kinetic parameters of in vitro fermentation of roughage and concentrated food used for feeding ruminants. Samples were prepared using 200, 300, 400, and 500 mg of air-dried roughage and concentrated sample, ground to 1 mm, and placed in 120 mL glass flasks. Next, inoculum and McDougal solution were added, and the readings were obtained using a semi-automated pressure transducer up to 96 h after the beginning of the incubations. Gas production of the non-fibrous fraction increased linearly (P < 0.05) for sugarcane, Marandu grass silage, corn silage, dried corn distillers’ grains with solubles, dried brewer’s yeast, bean residue, wet brewer’s grains, sunflower meal, and Jatropha meal; quadratically (P < 0.05) for Napier grass silage and cottonseed meal; and cubically (P < 0.05) for castor meal and soybean meal. The degradation rate of the non-fibrous fraction reduced linearly (P < 0.05) for sugarcane, Napier grass silage, and castor meal; quadratically (P < 0.05) for Marandu grass silage; and cubically (P < 0.05) for corn silage, soybean meal, dried corn distillers’ grains with solubles, bean residue, and cottonseed meal. Gas production of the fibrous fraction increased linearly (P < 0.05) for Napier grass silage, Marandu grass silage, corn silage, dried corn distillers’ grains with solubles, bean residue, wet brewer’s grain, cottonseed meal, and sunflower meal; quadratically ( < 0.05) for Jatropha meal; and cubically (P < 0.05) for sugarcane, castor meal, and soybean meal. The degradation rate of the fibrous fraction increased linearly (P < 0.05) for Napier grass silage, dried corn distillers’ grains with solubles, dried brewer’s yeast, wet brewer’s grains; quadratically (P < 0.05) for corn silage and castor meal; and cubically (P < 0.05) for sugarcane, Marandu grass silage, and bean residue. The lag time reduced linearly (P < 0.05) for castor meal and dried corn distillers’ grains with solubles; quadratically (P < 0.05) for Napier grass silage; and cubically (P < 0.05) for sugarcane, Marandu grass silage, corn silage, soybean meal, bean residue, cottonseed meal, sunflower meal, and Jatropha meal. Thus, our findings suggest that the kinetic parameters of in vitro fermentation were affected as a function of the amount of incubated sample.

scholarly journals Mycelial biomass cultivation of Lentinus crinitus

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Itaruã Machri Colla ◽  
Olavo Bilac Quaresma de Oliveira Filho ◽  
Janyeli Dorini Silva de Freitas ◽  
Míria Benetati Delgado Bertéli ◽  
Giani Andrea Linde ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Malt Extract ◽  
Biomass Growth ◽  
Mycelial Biomass ◽  
Protein Nitrogen ◽  
Temperature Growth ◽  
Nitrogen Concentrations ◽  
High Concentrations

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

UREA IN CORN SILAGE AS A SUPPLEMENTAL NITROGEN SOURCE FOR LACTATING COWS

1979 ◽  
Vol 59 (2) ◽  
pp. 403-410 ◽  
Author(s):  
F. D. SAUER ◽  
J. D. ERFLE ◽  
S. MAHADEVAN ◽  
J. R. LESSARD
Keyword(s):  
Crude Protein ◽  
Protein A ◽  
Daily Intake ◽  
Negative Control Group ◽  
Ammonia Nitrogen ◽  
Negative Control ◽  
Corn Silage ◽  
Feed Consumption ◽  
Control Group ◽  
Protein Nitrogen

Thirty-two Holstein cows in second or later lactation were randomly allocated to four treatment groups within 7 to 10 wk postpartum. Treatment rations were fed ad libitum as a complete feed and consisted of a negative control group which was fed a corn-oats-barley concentrate-corn silage mixture (40:60) with 9.4% crude protein, a urea silage group fed the same grain concentrate mixed with corn silage that contained 0.6% urea (on a fresh weight basis) to give 12.5% total ration crude protein, a group fed a soybean meal concentrate mixed with corn silage (12.7% crude protein), and a group fed a 3% urea corn-oats-barley concentrate mixed with corn silage to give a 12.8% total ration crude protein. The three groups supplemented with protein or non-protein nitrogen had greater weight gains, feed consumption, milk yields, milk persistencies, rumen ammonia nitrogen concentrations and greater rumen microbial cell populations than the negative control group. The results indicate that urea supports milk production when fed as part of a complete feed. Previous work showed that the same daily intake of urea when fed twice daily as part of the concentrate was ineffective.

The effects of dietary nitrogen level on the collagen of rat skin

1978 ◽  
Vol 39 (1) ◽  
pp. 181-192 ◽  
Author(s):  
R. Dawson ◽  
G. Milne
Keyword(s):  
Crude Protein ◽  
Collagen Content ◽  
Male Rats ◽  
Control Group ◽  
Protein Nitrogen ◽  
Total N ◽  
Dietary Nitrogen ◽  
Low Protein ◽  
Skin Collagen ◽  
Commercial Stock

1. Male rats of approximately 120 g body-weight were maintained on a commercial stock diet containing 204 g crude protein (nitrogen × 6.25)/kg, a hydroxyproline-free high-protein (HP) diet containing 200 g casein/kg as the only protein source, or a low-protein (LP) diet containing 40 g casein/kg. After 6 weeks on these diets half of each group was transferred to a non-protein (NP) diet and the experiment was continued for a further 6 weeks. Animals from each group were killed at 4 d, 3 weeks and 6 weeks after the transfer to the NP diet.2. Throughout the experiment the urinary excretion of N, hydroxyproline and creatinine, and the content and solubility of the skin collagen were determined.3. When compared with a control group killed at the beginning of the experiment the rats maintained on the LP diet showed an increase of 25% in total N content of the skin but collagen content increased by 100%. Rats transferred from the HP to the NP diet lost both N and collagen from the skin, but those transferred from the LP to the NP diet lost N but increased the collagen content by 42%.4. Protein deprivation brought about marked changes in the solubility of the skin collagen, suggesting an increase in the rate of maturation of skin collagen.

ENSILING CHARACTERISTICS OF SILAGES TREATED WITH SODIUM HYDROXIDE

1976 ◽  
Vol 56 (4) ◽  
pp. 935-940 ◽  
Author(s):  
PAUL FLIPOT ◽  
D. N. MOWAT ◽  
J. G. BUCHANAN-SMITH ◽  
J. J. PARKINS
Keyword(s):  
Corn Stover ◽  
Dry Matter ◽  
Alkali Treatment ◽  
Lactic Acid Production ◽  
Corn Silage ◽  
Total N ◽  
Whole Plant ◽  
Corn Stover Silage ◽  
On Farm ◽  
Farm System

Two trials were conducted to investigate the effect of NaOH on the ensiling characteristics of corn and alfalfa forage. In one trial, NaOH was added at levels of 0, 2, 3, or 4% of dry matter (DM) to whole plant corn and 0 or 3% to corn stover, and ensiled in cryovac bags. In a second trial, 0 or 3% NaOH was added to whole plant corn or alfalfa (25–40% DM). In addition, alfalfa had molasses added at a level of 0 or 10% of the forage DM. Materials were ensiled in miniature silos of 250-ml capacity. Additions of NaOH to all forages increased silage pH (P < 0.05). Molasses may have some influence in limiting the rise in pH after addition of NaOH but the effect appears to be limited. Alkali treatment of corn stover silage and corn silage generally increased lactic acid production. Total-N of all silages tended to decrease with NaOH treatment. However, alkali treatment markedly reduced soluble-N with all forages (P < 0.05), except with corn stover. Molasses also reduced soluble-N (P < 0.05) with alfalfa silage of low DM content. Treating forages while ensiling would appear to represent a suitable on-farm system for alkali treatment.

RESPONSE OF LACTATING DAIRY COWS TO FORMALDEHYDE-TREATED SOYBEAN MEAL WHEN FED WITH CONTROL OR UREA-TREATED CORN SILAGE

1986 ◽  
Vol 66 (1) ◽  
pp. 85-95 ◽  
Author(s):  
J. D. ERFLE ◽  
F. D. SAUER ◽  
S. MAHADEVAN ◽  
R. M. TEATHER
Keyword(s):  
Body Weight ◽  
Dairy Cows ◽  
Milk Production ◽  
Soybean Meal ◽  
Dry Matter ◽  
Milk Protein ◽  
Rumen Fluid ◽  
Amino Acid Content ◽  
Negative Control ◽  
Corn Silage

In two experiments, lactating cows (26 in exp. 1; 32 in exp. 2) were fed an 11.3% crude protein (CP) diet, a 14.7% CP diet containing untreated soybean meal (SBM) or a 14.6% CP diet containing formaldehyde-treated SBM (FSBM), all three fed as a complete blended diet with control corn silage (CCS) (exp. 1); a 12% CP diet, a 13.4% CP diet containing SBM or a 13.3% CP diet containing FSBM, all fed as a complete blended diet with urea-treated corn silage (UCS) (exp. 2). In both experiments concentrates and CCS or UCS were mixed (50:50 dry matter) daily and fed ad lib for a 16-wk period. The increase of CP over the low CP negative control by addition of untreated SBM resulted in a significant (P < 0.05) increase in milk production in both experiments. The addition of FSBM had no significant effect. When comparing solids-corrected milk (SCM), addition of SBM, whether treated or not, significantly increased SCM in both experiments. In neither experiment did FSBM improve milk production over untreated SBM. All animals fed CCS lost body weight but there was no difference between treatments. For animals fed UCS the low CP group lost significantly (P < 0.05) more body weight than did the group fed FSBM. Dry matter (DM) intake was significantly (P < 0.05) lower for the FSBM fed group than for the group fed untreated SBM in exp. 1 (CCS). DM intake was significantly (P < 0.05) increased by either FSBM or SBM over the low CP control fed UCS. Milk protein was the only component significantly (P < 0.05) decreased by FSBM in exp. 1. Isobutyric and isovaleric acids were significantly (P < 0.05) lower in rumen fluid of animals on FSBM plus CCS. Rumen microbial protein content seemed to be more adversely affected by FSBM with CCS than UCS. Amino acid content of FSBM and SBM demonstrated that lysine and tyrosine were lost from SBM after treatment with formaldehyde. The lack of a response in milk production to FSBM is discussed in terms of a lysine and tyrosine deficiency for milk protein synthesis and the adverse effect that formaldehyde protection of SBM may have on the rumen microbial population. Key words: Lactation, dairy cows, urea, corn silage, soybean meal, formaldehyde treatment

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