VARIATION IN FATTY ACID COMPOSITION AMONG LIVER, PERIRENAL FAT, AND RIB TISSUES OF STEER CARCASSES

1965 ◽  
Vol 45 (1) ◽  
pp. 29-32 ◽  
Author(s):  
W. K. Roberts

Tissue samples (liver, perirenal fat, and non-bone tissue from 9th to 11th rib cut) were obtained from carcasses of Hereford steers that had been fattened in two different groups during two consecutive years, and were analyzed for fatty acids by gas–liquid chromatography. Myristic acid was significantly (P < 0.01) lower in liver than perirenal or rib tissues (non-bone tissue from 9th to 11th rib cut). Palmitic acid levels were significantly (P < 0.01) different among the tissues in one group, but not in the other. Palmitoleic acid was found in all areas with the highest level, within groups, occurring in rib tissue (av. 4.8%). Perirenal fat contained significantly (P < 0.01) more stearic acid than either rib or liver tissues and oleic acid was significantly (P < 0.01) higher in rib than in the other tissues. The liver tissue contained approximately 9.1% linoleic acid, which was significantly (P < 0.01) higher in perirenal or rib tissues (less than 2%). Linolenic and arachidonic acids were detected in liver, but not in the other tissues.

Author(s):  
R.M. Papaev ◽  
◽  
G.G. Shalamova ◽  
T.Yu. Motina ◽  
◽  
...  

The article presents the results of studies of the fatty acid composition of the muscle tissue of barbs at the age of 4 and 8 months. It was established by gas-liquid chromatography that the composition contained saturated and mono- and polyunsaturated fatty acids: palmitic, palmitoleic, stearic, arachidic, cis-linoleic, cis-oleic, alpha-linolenic, erucic and selacholic. In adults, in compari-son with juveniles, the content of palmitic and stearic acids was higher by 21.6 and 17.7 %. In barbs at the age of 4 months, in comparison with adults, the content of palmitoleic acid was lower by 42.6 %, cis-oleic acid by 10.4 %, cis-linoleic acid – by 9.3 % and selacholic acid – by 8.3 %. In juvenile barbs, alpha-linoleic and erucic acids were absent, and in adults at the age of 8 months, arachidic acid was absent.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 232-233
Author(s):  
Ekaterina Karpova ◽  
Ludmila Chizhova ◽  
Eugenia Surzhikova

Abstract The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.


1970 ◽  
Vol 42 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Md Moshfekus Saleh-E-In ◽  
Sudhangshu Kumar Roy

Anethum sowa L. (Dill) seeds were investigated to determine the fatty acid composition and proximate analyses. The seeds contain 9.36 % fatty oil. The saturated and unsaturated fatty acids contributed 6.22% and 93.78% respectively of the oil. The per cent composition of the extracted oil was identified by Gas Liquid Chromatography (GLC). Among the six fatty acids identified from this study oleic acid contributed the highest proportion (87.10%), where as, linolenic, palmitic, stearic, behenic and arachidic all together contributed the rest (12.90%). Proximate analyses showed that A. sowa. seeds are good source of dietary fibre. Overall Dill seeds oil can be considered as a good source of oleic acid. Key words: Anethum sowa, dill seed oil, fatty acid composition, oleic acid, linolenic acid, Gas liquid chromatography. Bangladesh J. Sci. Ind. Res. 42(4), 455-464, 2007


1965 ◽  
Vol 97 (2) ◽  
pp. 371-374 ◽  
Author(s):  
JD Sink ◽  
CK Huston ◽  
JW Shigley

1. The effect of diethylstilboestrol on the fatty acid composition of adipose-tissue lipids of the ox (Bos taurus) was studied. 2. The capsula adiposa (perirenal) was shown to contain more total saturated fatty acids, whereas more total unsaturated fatty acids were found in the panniculus adiposus (subcutaneous). 3. Significantly more stearic acid and linolenic acid were obtained from the capsula adiposa, whereas the panniculus adiposus contained more myristoleic acid, palmitoleic acid and oleic acid. 4. Implanting diethylstilboestrol significantly increased the deposition of the saturated fatty acids, particularly stearic acid. 5. A decrease in the deposition of total unsaturated fatty acids, myristoleic acid, palmitoleic acid and linoleic acid can also be attributed to the diethylstilboestrol treatment.


1970 ◽  
Vol 46 (1) ◽  
pp. 127-132 ◽  
Author(s):  
BK Paul ◽  
MMU Munshi ◽  
MN Ahmed ◽  
GC Saha ◽  
SK Roy

The fresh rhizomes of Curcuma longa Linn. (Turmeric or Holud) collected from three different places of Bangladesh were investigated to extract oil, its fatty acid composition and its physico-chemical properties. The rhizomes contained 8.76 - 10.92% oil. The percentage compositions of fatty acids were identified and quantified by GLC. The saturated and unsaturated fatty acid contents of three places were found to vary within 22.25 - 23.44% and 76.11 - 77.59%, respectively. Among identified six fatty acids, oleic acid contributed the highest proportion (56.24 - 58.88%), followed by myristic acid (16.25 - 17.71%); whilst, palmitic (5.59 - 6.00%), linoleic (10.90 - 12.82%), linolenic (4.15 - 5.46%) and ecosenoic acid (2.72 - 3.25%) together contributed the rest. Physico - chemical properties of the extracted oil were also investigated. The specific gravity, refractive index, optical rotation were recorded as 0.892 to 0.919 at 30°C, 1.431 to 1.465 at 30°C and +11.54° to +13.56° at 26°C, respectively. The chemical properties like saponification value (195.23 - 205.33), iodine value (75.53 - 90.47), peroxide value (23.25 - 36.16), acid value (11.08 - 11.32), ester value (56.30 - 64.13) and percentage of unsaponifiable matter (8.31 - 15.04%) were determined. Overall fresh Turmeric oil can be considered as a good source of oleic acid. Keywords: Curcuma longa; Fresh turmeric oil; Fatty acid composition; Oleic acid; Gas liquid chromatography. DOI: http://dx.doi.org/10.3329/bjsir.v46i1.8116 Bangladesh J. Sci. Ind. Res. 46(1), 127-132, 2011


Author(s):  
Masatoshi Mita ◽  
Mariko Deguchi ◽  
Yuichi Sasayama

A large quantity of triglyceride was detected histochemically in nutrient-deposit cells of the trophosome in the beard worm (Oligobrachia mashikoi). The lipid extracted from the trophosome was composed of triglyceride, several kinds of phospholipid, free fatty acid, cholesterol, and cholesterol ester. The fatty acid of the triglyceride was comprised mainly of a monoenoic type, such as palmitoleic acid (16:1) and oleic acid (18:1), which accounted for 31% and 37% of the total fatty acids, respectively. In addition, in blood triglyceride, the fatty acid composition was almost the same.


Author(s):  
Susma Giri ◽  
Michael Dillon

Fatty acids (Fas), the most important energy resources in insects, may change in structure and thus function with changing temperature, a hypothesis termed ‘homeoviscous adaptation’. We investigated whether the proportional composition of the most common fatty acids changes with seasonal (June to August) and altitudinal (2060 – 3290 m) variation in environmental temperature among four species of native bees. We identified the composition and proportion of each fatty acid using gas chromatography coupled with a flame ionization detector (GC-FID). Based on preliminary data, the most common fatty acids found in bees were palmitic acid (C 16:0), stearic acid (C 18:0), oleic acid (C 18:1), linoleic acid (C 18:2) and linolenic acid (C 18:3), with other fatty acids including myristic acid (C 14:0) and palmitoleic acid (C 16:1) also present in small amounts. We are currently 23eticulat GC data for larger bees and establishing protocols for fatty acid composition analysis of small tissue samples. Based on the seasonal and altitudinal variation in ambient temperature, we expect to see variation in fatty acid proportions in bees from different months at both sites.


1970 ◽  
Vol 46 (1) ◽  
pp. 111-116
Author(s):  
BK Paul ◽  
MN Ahmed ◽  
GC Saha

Carum roxburghianum Benth. (Radhuni) seeds of three different places of Bangladesh were investigated to determine the fatty acid composition and physico-chemical properties of extracted oil. The seeds were found to contain oil ranging from 15.31 to 20.32%. The percentage compositions of fatty acids were identified and quantified by Gas Liquid Chromatography (GLC). The saturated and unsaturated fatty acids in extracts were 4.95 to 6.27% and 93.73 to 94.89%, respectively. Among six fatty acids identified in this study, oleic acid contributed the highest portion (76.44 to 79.16%), whereas, linoleic (1.38%), linolenic (15.28 - 15.40%), stearic (0.70%), palmitic (4.95 - 5.57%) and ecosenoic acid (0.51 - 15.73%) together contributed the rest. Physico-chemical properties of the oil extracted were also investigated. The specific gravity, refractive index, optical rotation were recorded as 0.903 to 0.918 at 30°C, 1.465 to 1.470 at 30°C and +8.54° to +9.56° at 26°C, respectively. The chemical properties like saponification value (183.52 - 193.45), iodine value (80.99 - 120.90), peroxide value (23.25 - 36.16), acid value (143.84 - 162.99), ester value (48.12 - 62.03), percentage of unsaponifiable matter (3.56 - 6.51%), Reicher-Meissel value (2.00 - 3.12), Polenske value (4.12 - 6.20) and Henher value (85.12 - 95.56) were determined. Overall Radhuni seeds oil can be considered as a good source of oleic acid. Keywords: Carum roxburghianum; Radhuni seed oil; fatty acid composition; oleic acid; Gas Liquid Chromatography. DOI: http://dx.doi.org/10.3329/bjsir.v46i1.8113 Bangladesh J. Sci. Ind. Res. 46(1), 111-116, 2011


1966 ◽  
Vol 33 (3) ◽  
pp. 255-259 ◽  
Author(s):  
W. R. H. Duncan ◽  
G. A. Garton

SummaryThe fatty acid composition of colostral and milk fat samples representative of the total secretion of the nursing mammae of each of 3 sows was determined by gas-liquid chromatography. In both colostral and milk fats, acids of chain length C16 and C18 accounted for more than 90% of the total fatty acids present; colostral fat contained a higher proportion of oleic acid and linoleic acid and less palmitic acid and palmitoleic acid than normal milk fat. Structural studies on milk triglycerides showed that, as in pig depot fat, palmitic acid is preferentially esterified to the secondary alcoholic group (2-position) of the glycerol molecules with the C18 acids (stearic, oleic and linoleic acids) being present for the most part in the 1- and 3- positions.


1994 ◽  
Vol 300 (2) ◽  
pp. 509-518 ◽  
Author(s):  
P C Calder ◽  
P Yaqoob ◽  
D J Harvey ◽  
A Watts ◽  
E A Newsholme

The fatty acid compositions of the neutral lipid and phospholipid fractions of rat lymph node lymphocytes were characterized. Stimulation of rat lymphocytes with the T-cell mitogen concanavalin A resulted in significant changes in the fatty acid composition of both neutral lipids and phospholipids (a decrease in the proportions of stearic, linoleic and arachidonic acids and an increase in the proportion of oleic acid). Membrane fluidity was measured using nitroxide spin-label e.s.r., and increased during culture with concanavalin A. Culturing the lymphocytes in the absence of mitogen did not affect fatty acid composition or membrane fluidity. The uptake and fate of palmitic, oleic, linoleic and arachidonic acids were studied in detail; there was a time-dependent incorporation of each fatty acid into all lipid classes but each fatty acid had a characteristic fate. Palmitic and arachidonic acids were incorporated principally into phospholipids whereas oleic and linoleic acids were incorporated in similar proportions into phospholipids and triacylglycerols. Oleic acid was incorporated mainly into phosphatidylcholine, palmitic and linoleic acids were incorporated equally into phosphatidylcholine and phosphatidylethanolamine, and arachidonic acid was incorporated mainly into phosphatidylethanolamine. Supplementation of the culture medium with particular fatty acids (myristic, palmitic, stearic, oleic, linoleic, alpha-linolenic, arachidonic, eicosapentaenoic or docosahexaenoic acid) led to enrichment of that fatty acid in both neutral lipids and phospholipids. This generated lymphocytes with phospholipids differing in saturated/unsaturated fatty acid ratio, degree of polyunsaturation, index of unsaturation and n - 6/n - 3 ratio. This method allowed the introduction into lymphocyte phospholipids of fatty acids not normally present (e.g. alpha-linolenic) or usually present in low proportions (eicosapentaenoic and docosahexaenoic). These three n - 3 polyunsaturated fatty acids replaced arachidonic acid in lymphocyte phospholipids. Fatty acid incorporation led to an alteration in lymphocyte membrane fluidity: palmitic and stearic acids decreased fluidity whereas the unsaturated fatty acids increased fluidity. It is proposed that the changes in lymphocyte phospholipid fatty acid composition and membrane fluidity brought about by culture in the presence of polyunsaturated fatty acids are responsible for the inhibition of lymphocyte functions caused by these fatty acids.


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