THE ADDITION OF BUFFERS TO RUMINANT RATIONS: IV. THE EFFECT OF ADDITIONS OF SODIUM BICARBONATE, SODIUM PROPIONATE, LIMESTONE AND COD LIVER OIL ON INTRA-RUMEN ENVIRONMENT

1963 ◽  
Vol 43 (2) ◽  
pp. 309-319 ◽  
Author(s):  
J. W. G. Nicholson ◽  
H. M. Cunningham ◽  
D. W. Friend
Keyword(s):  
Acetic Acid ◽  
Sodium Bicarbonate ◽  
Propionic Acid ◽  
Rumen Fluid ◽  
Linseed Oil ◽  
Buffering Capacity ◽  
Cod Liver Oil ◽  
Sodium Propionate ◽  
Cent Sodium ◽  
Basal Ration

Two experiments with fistulated steers were designed to study the effects of adding 5.7 per cent sodium bicarbonate, 5.7 per cent ground limestone, and 5.7 per cent sodium bicarbonate plus either 3.6 per cent sodium propionate or 60 milliliters per day of cod liver oil to an all-concentrate basal ration composed of barley, oats, linseed oil meal, molasses, minerals and vitamins A and D2. The effects of using rolled or ground grains were also studied. All of the buffered rations, except that containing limestone, resulted in a higher rumen pH and buffering capacity than did the basal ration. The buffers had little effect on the total concentration but did result in changes in the molar proportions of the VFA in rumen fluid. In both experiments, the buffered rations, except that containing limestone, gave a lower molar per cent of acetic acid than did the basal ration. The addition of either cod liver oil or sodium propionate to the sodium bicarbonate ration resulted in a higher molar per cent of the propionic acid than with the basal ration. The butyric acid concentration was lower for the ration containing cod liver oil than for either the basal or sodium bicarbonate rations. Rolled grains in the basal ration gave less extreme results than did ground grains; however, the differences were not significant.Samples taken at 3, 5, 8 and 16 hours after feeding showed marked diurnal variations in all criteria studied, except the proportion of acetic acid. Values obtained at 3 and 5 hours after feeding showed little change but thereafter there was a decrease in concentration of total VFA and the proportion of propionic acid and an increase in pH, buffering capacity and the proportions of butyric and the higher acids. Interactions between the rations and times of sampling show that the buffers are most effective in modifying the intra-rumen environment shortly after feeding but this effect had partly disappeared by 5 hours after feeding.

THE ADDITION OF BUFFERS TO RUMINANT RATIONS: II. ADDITIONAL OBSERVATIONS ON WEIGHT GAINS, EFFICIENCY OF GAINS AND CONSUMPTION BY STEERS OF ALL-CONCENTRATE RATIONS

1962 ◽  
Vol 42 (1) ◽  
pp. 75-81 ◽  
Author(s):  
J. W. G. Nicholson ◽  
D. W. Friend ◽  
H. M. Cunningham
Keyword(s):  
Sodium Bicarbonate ◽  
Feed Efficiency ◽  
Linseed Oil ◽  
Feed Consumption ◽  
Beef Steers ◽  
Cod Liver Oil ◽  
Sodium Propionate ◽  
High Incidence ◽  
Cent Sodium ◽  
Kidney Lesions

Two experiments, involving a total of 60 beef steers fed all-concentrate rations, were conducted to determine the effect of additions of sodium bicarbonate, sodium propionate and cod liver oil on rates of gain, feed intake, feed efficiency and carcass grades of the steers. The basal ration consisted of the following ingredients in percentages: barley 40; oats 40; linseed oil meal 10; molasses 8.5; dicalcium phosphate 1.0; trace-mineralized salt 0.5; plus vitamins A and D. The addition of 5.7 per cent sodium bicarbonate to this all-concentrate ration, composed of either ground or rolled grains, did not improve gains, feed efficiency or carcass grades. The addition of 3.6 per cent sodium propionate to the bicarbonate-containing ration did not have any significant effect on animal gains, feed efficiency or carcass grades. When 75 milliliters of cod liver oil was fed once daily with a ration containing 5.7 per cent sodium bicarbonate, carcass grade, dressing percentage, feed consumption and rate of gain, but not feed efficiency, were significantly reduced. The rolled-grain rations gave more satisfactory results than the ground-grain rations. A high incidence of kidney lesions was observed among some of the ration groups.

THE ADDITION OF BUFFERS TO RUMINANT RATIONS: III. THE EFFECT OF ADDITIONS OF SODIUM BICARBONATE, SODIUM PROPIONATE, LIMESTONE AND COD LIVER OIL ON APPARENT DIGESTIBILITY AND NITROGEN RETENTION OF AN ALL-CONCENTRATE RATION

1962 ◽  
Vol 42 (1) ◽  
pp. 82-87 ◽  
Author(s):  
J. W. G. Nicholson ◽  
D. W. Friend ◽  
H. M. Cunningham
Keyword(s):  
Organic Matter ◽  
Sodium Bicarbonate ◽  
Dry Matter ◽  
Latin Square ◽  
Nitrogen Retention ◽  
Cod Liver Oil ◽  
Sodium Propionate ◽  
Cent Sodium ◽  
Organic Matter Digestibility ◽  
Design Experiments

Rumen-fistulated yearling steers were used in two 4 × 4 latin square design experiments to determine the digestibility and nitrogen retention of all-concentrate rations with various supplements. The inclusion of 5.7 per cent sodium bicarbonate in either a ground- or dry-rolled-grain ration resulted in an increase in urine excretion. Digestibility of organic matter and nitrogen or nitrogen retention were not affected. The addition of 60 milliliters of cod liver oil (1200 I.U. vitamin A, 150 I.U. vitamin D per gram) per day to the bicarbonate supplemented, ground-grain ration tended to decrease organic matter digestibility while the addition of 3.6 per cent sodium propionate tended to increase organic matter digestibility. Differences between these rations and the bicarbonate ration were not statistically significant; however, the percentage of nitrogen retained on the cod liver oil supplemented ration was significantly (P < 0.05) lower than on the propionate supplemented ration.The inclusion of 5.7 per cent ground limestone significantly (P < 0.05) reduced the digestibility of dry matter, and nitrogen, but the digestibility of organic matter was not significantly lower at this probability level.

scholarly journals Comparison of grass silage utilization by reindeer and sheep: 2. Rumen fermentation and rumen microbiota

1982 ◽  
Vol 54 (2) ◽  
pp. 127-135
Author(s):  
Liisa Syrjälä-Qvist
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Rumen Microbiota ◽  
Grass Silage ◽  
Rumen Ph ◽  
Sampling Times

The criteria used in comparing the utilization of grass silage by reindeer and sheep were rumen pH, ammonia, volatile fatty acids (VFA) and microbes. Rumen samples were taken before feeding, and 2 ½ and 5 ½ hours after the beginning of feeding. Rumen fermentation was lower in the reindeer than in the sheep and differed less between the three sampling times. In the reindeer/the pH of the rumen fluid averaged 6.94 and in the sheep 6.61. The average amounts of NH3—N were 17.0 and 24.2 mg/100 ml rumen fluid and those of total VFA 8.46 and 10.90 mmoles/100ml rumen fluid, respectively. The proportion of acetic acid in the VFA in the reindeer was 75.3 molar % and in the sheep 66.0 molar %, the corresponding values for propionic acid being 18.5 and 22.0 molar % and for butytic acid 4.2 and 8.8 molar %. The number of rumen ciliates in the reindeer averaged 87/mm3 rumen contents and in the sheep 314/ mm3. The numbers of bacteria were 16.0 X 106/mm3, respectively. The proportion of the total microbe mass in the reindeer rumen contents was 1.8 % and in the sheep 2.4 %. The proportions of bacteria in this mass were 87 % and 70 %, respectively. The differences between the reindeer and sheep in the rumen fermentation results and in the numbers of rumen microbiota were nearly all statistically significant (P

Fermentation in the Rumen of the Sheep

1951 ◽  
Vol 28 (1) ◽  
pp. 74-82
Author(s):  
F. V. GRAY ◽  
A. F. PILGRIM ◽  
R. A. WELLER
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Rumen Fluid ◽  
Rumen Fermentation ◽  
Natural Process ◽  
The Other ◽  
Rapid Absorption

1. When wheaten hay and lucerne hay were fermented by organisms from the rumen of the sheep it was necessary to employ a large inoculum of rumen fluid in order to reproduce the rumen fermentation in vitro. With a small inoculum the fermentation did not conform to the known characteristics of the natural process. 2. Products per kilogram of wheaten hay fermented in vitro were: fatty acids 200-250 g.--acetic acid 41%, propionic acid 43% and butyric acid 16% (by weight); methane 15 l. Products per kilogram of lucerne hay were: fatty acids 250-300 g.--acetic acid 53%, propionic acid 29% and butyric acid 18% (by weight); methane 20 l. 3. The findings support the view that, owing to the more rapid absorption of propionic than of the other acids from the rumen, the proportion of this acid remaining in the rumen fluid is considerably less than the proportion actually formed in the fermentation.

THE EFFECT OF ORGANIC ACID SALTS ON GROWTH AND RUMEN VOLATILE FATTY ACIDS OF SHEEP FED PELLETED OR CHOPPED HIGH-ROUGHAGE RATIONS

1964 ◽  
Vol 44 (1) ◽  
pp. 58-67 ◽  
Author(s):  
J. W. G. Nicholson ◽  
H. M. Cunningham
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Volatile Fatty Acids ◽  
Sodium Citrate ◽  
Rumen Fluid ◽  
Feed Consumption ◽  
Sodium Propionate ◽  
Ad Libitum ◽  
The One ◽  
Standard Ration

Four experiments were conducted with sheep to compare the addition of sodium citrate and sodium propionate to pelleted and unpelleted rations and to determine the response to sodium citrate supplementation of different quality-hays. There were no significant differences in rate of gain or feed consumption of a ⅔ roughage −⅓ concentrate ration due to supplementation with sodium citrate or sodium propionate. Pelleting either the mixed ration or all-roughage rations increased feed consumption and rate of gain in all experiments. Pelleting the mixed ration decreased digestibility of dry matter, organic matter, and nitrogen in the one experiment in which this was determined. Pregnant and lactating ewes lost less body weight when fed a limited amount of long hay plus pelleted hay ad libitum than similar ewes fed a standard ration of long hay ad libitum plus 0.5 lb of concentrates per day. It was necessary to feed lambs 0.5 lb of rolled barley per day with long hay ad libitum to obtain gains equal to those of similar lambs fed pelleted hay ad libitum. The addition of sodium citrate significantly increased the rate of gain of lambs fed ad libitum a slightly moldy, weathered legume hay in the pelleted form together with 0.25 lb of long hay per head per day.The addition of sodium citrate to a mixed ration or to all-roughage rations did not usually alter the proportions of rumen volatile fatty acids. The addition of sodium propionate decreased the proportion of acetic acid while increasing that of propionic acid. In both experiments where this was determined, pelleting an all-roughage ration resulted in a decrease in the proportion of acetic acid and an increase in the longer chain volatile fatty acids in rumen fluid.

scholarly journals The influence of ruminal infusion of volatile fatty acids on milk yield and composition and on energy utilization by lactating cows

1969 ◽  
Vol 23 (3) ◽  
pp. 443-453 ◽  
Author(s):  
E. R. Ørskov ◽  
W. P. Flatt ◽  
P. W. Moe ◽  
A. W. Munson ◽  
R. W. Hemken ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Acid Infusion ◽  
Body Tissues ◽  
Basal Ration

1. In an experiment of 3 x 3 latin square design, four lactating Holstein cows were given a basal ration designed to induce low percentages of milk fat. The treatments were (I) basal ration, a pelleted mixture of lucerne hay (20%) and concentrates (80%), with 40 l. of water infused intraruminally, (2) basal ration with acetic acid substituted for 15.4% of the metabolizable energy (ME) and (3) propionic acid substituted for 15.4% of the ME. In the last 3 weeks of the 6-week experimental period respiration trials were carried out in an open-circuit indirect calorimeter. The levels of feeding offered in the three periods were 325, 275 and 225 kcal ME/kg body-weight 0.75 in periods 1, 2 and 3 respectively.2. No differences were detected in the utilization of the energy of acetic and propionic acids, but there were differences in the partition of energy into milk or body tissues; with acetic acid infusion more energy was secreted as milk and with propionic acid infusion more was deposited in body tissue.3. There was an increase in milk fat percentage with acetic acid infusion, but not complete recovery to normal. The milk fat percentages were 1.96, 2.58 and 1.92 for treatments 1, 2 and 3 respectively. Acetic acid infusion caused increases in the C12, C14 and C16 fatty acids of milk fat and decreased the proportion of C18:1 fatty acids.4. It is suggested that the low percentages of milk fat found when cows are given concen- trates could result from a decreased extent of fermentation in the rumen, allowing a greater proportion of the starch consumed to be absorbed as glucose in the small intestine.

The buffering capacity of caecal digesta exceeds that of rumen digesta from sheep fed pasture or roughage diets

1997 ◽  
Vol 48 (5) ◽  
pp. 723 ◽  
Author(s):  
Z. Ding ◽  
J. B. Rowe ◽  
I. R. Godwin ◽  
Y. Xu
Keyword(s):  
Acetic Acid ◽  
Sodium Bicarbonate ◽  
Organic Acids ◽  
Phosphate Buffer ◽  
Buffering Capacity ◽  
Sheep Grazing ◽  
Factors Affecting ◽  
Sodium Dihydrogen ◽  
Sodium Dihydrogen Orthophosphate ◽  
Dihydrogen Orthophosphate

The buffering capacities of caecal and rumen digesta of sheep on different diets were determined by titration with lactic, acetic, and hydrochloric acids, and certain factors affecting the buffering capacity of rumen digesta were studied. Both rumen and caecal digesta had maximal buffering capacity at pH 6·5–6·0. The buffering capacity of caecal digesta was nearly double (P < 0·001) that of rumen digesta. The rumen digesta from sheep fed oaten chaff had a buffering capacity 21% higher (P < 0·05) than that of sheep grazing green pasture. This was reduced (P < 0·05) by one-third following ruminal infusions of glucose, lactic, or acetic acid to induce acidosis. Diet did not significantly affect the buffering capacities of rumen and caecal digesta. However, the buffering capacities of rumen and caecal digesta from pasture-fed sheep that had been fasted for 24 h were significantly greater (P < 0·001) than those for sheep that had not been fasted (62 and 18%). The buffering capacity determined using HCl was always less than that for lactic or acetic acid. This may be due to the lower pKa for HCl and the fact that there is no evidence that HCl undergoes inter-conversion through fermentation that the organic acids may undergo. The addition of carbonate or phosphate buffer significantly increased (P < 0·05) the buffering capacity of rumen and caecal digesta. The sodium bicarbonate and sodium carbonate (NaHCO3 and Na2CO3) system played a more effective buffering role than the sodium dihydrogen orthophosphate and disodium hydrogen orthophosphate (NaH2PO4 and Na2HPO4) system in the rumen digesta.

Excess molar volumes of binary mixtures of acetic acid and propionic acid with some members of homologous series of alkanes

1991 ◽  
Vol 56 (4) ◽  
pp. 736-744 ◽  
Author(s):  
Ondřej Drábek ◽  
Ivan Cibulka
Keyword(s):  
Acetic Acid ◽  
Propionic Acid ◽  
Alkyl Chain ◽  
Excess Molar Volumes ◽  
Liquid Mixtures ◽  
Entire Concentration Range ◽  
Molar Volumes ◽  
Binary Liquid ◽  
Alkane Molecule ◽  
Increasing Temperature

Excess molar volumes of binary liquid mixtures of (acetic or propionic acid = hexane) at 25 and 35°C, and (acetic or propionic acid + heptane or octane) and (acetic acid + dodecane) at 25°C, measured with a tilting dilution dilatometer, are reported. The excess volumes are positive over the entire concentration range for all mixtures and increase with increasing length of an alkane molecule, decrease with increasing of the alkyl chain in a molecule of carboxylic acid, and increase with increasing temperature.

Vapour-liquid equilibrium in the ternary system cyclohexane-acetic acid-propionic acid

1989 ◽  
Vol 54 (11) ◽  
pp. 2840-2847 ◽  
Author(s):  
Ivona Malijevská ◽  
Alena Maštalková ◽  
Marie Sýsová
Keyword(s):  
Acetic Acid ◽  
Ternary System ◽  
Propionic Acid ◽  
Activity Coefficients ◽  
Error Propagation ◽  
Analytical Techniques ◽  
Liquid Equilibrium ◽  
Consistency Test ◽  
Propagation Law ◽  
Equilibrium Data

Isobaric equilibrium data (P = 101.3 kPa) for the system cyclohexane-acetic acid-propionic acid have been measured by two different analytical techniques. Activity coefficients calculated by simultaneous solving of equations for the chemical and phase equilibria were subjected to a consistency test based on inaccuracies determined from the error propagation law, and were correlated by Wilson’s equation. The activity coefficients measured were compared with those calculated from binary vapour-liquid equilibrium data and with values predicted by the UNIFAC method.

scholarly journals Pilot Study of the Effects of Polyphenols from Chestnut Involucre on Methane Production, Volatile Fatty Acids, and Ammonia Concentration during In Vitro Rumen Fermentation

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 108
Author(s):  
Yichong Wang ◽  
Sijiong Yu ◽  
Yang Li ◽  
Shuang Zhang ◽  
Xiaolong Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Acid Content ◽  
Volatile Fatty Acids ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Quadratic Response ◽  
In Vitro Rumen Fermentation

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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