EFFECTS OF PORCINE IMMUNE GLOBULIN ADMINISTRATION ON THE SURVIVAL AND SERUM PROTEIN COMPOSITION OF COLOSTRUM-DEPRIVED PIGS REARED IN A NON-ISOLATED ENVIRONMENT

1961 ◽  
Vol 41 (2) ◽  
pp. 236-252 ◽  
Author(s):  
B. D. Owen ◽  
J. M. Bell ◽  
C. M. Williams ◽  
R. G. Oakes
Keyword(s):  
Oral Administration ◽  
Serum Protein ◽  
Immune Globulin ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Post Partum ◽  
Protein Composition ◽  
Paper Strip ◽  
Percentage Survival ◽  
Immune Globulins

Five experiments involving 90 newborn colostrum-deprived pigs were conducted in an attempt to develop a method of rearing applicable in a non-isolated environment. Immune globulins, prepared by ammonium sulphate fractionation of porcine serum, and comprised of a mixture of approximately 75 per cent γ-globulin and 25 per cent β-globulin, were administered orally or parenterally in varying amounts and for varying periods of time. In two experiments porcine albumin, in serum or in a semi-purified solution, was provided in addition to the immune globulins. The distribution of serum proteins in the pigs was studied from birth to 12 weeks of age by paper strip electrophoresis.Parenteral administration of immune globulins did not provide an effective passive immunity, nor did oral administration for 1 day post-partum. A marked improvement in survival occurred when oral administration was continued for 5 days, and it was further found that this treatment provided apparently complete protection against infection during the 5-day period of administration. Albumin appeared to further improve survival.These results, together with the relatively poor survival obtained with positive control pigs (nursed 24 hours) suggested a continuing need for a supply of immune globulins in the lumen of the intestinal tract. Presumably these globulins were active as coproantibodies.The percentage survival in pigs weighing 3 pounds or more at birth was substantially higher than in smaller pigs. Mortality in these experiments was usually attributable to colibacillosis.Serum immune globulin levels at 2 days of age in the artificially reared pigs were elevated in proportion to the amount of globulins given. The effect of albumin was to create a serum protein pattern resembling that of suckled pigs. A marked decline in γ-globulin levels from 2 days to approximately 6 weeks was observed.

FURTHER STUDIES OF SURVIVAL AND SERUM PROTEIN COMPOSITION IN COLOSTRUM-DEPRIVED PIGS REARED IN A NON-ISOLATED ENVIRONMENT

1964 ◽  
Vol 44 (1) ◽  
pp. 1-7 ◽  
Author(s):  
B. D. Owen ◽  
J. M. Bell
Keyword(s):  
Birth Weight ◽  
Serum Albumin ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Composition ◽  
Passive Immunity ◽  
Immune Globulins ◽  
Weight Effect ◽  
Experimental Treatments ◽  
Swine Serum

Forty-eight newborn colostrum-deprived pigs were studied in an experiment designed to provide further information regarding effects of immune globulin source (university herd vs. abattoir blood), period of globulin feeding (5 days vs. 10 days), albumin feeding, and birth weight on survival and serum protein composition.Orally administered immune globulins from either university or abattoir swine serum provided an effective passive immunity during the period of administration. This response was presumed to be partly attributable to coproantibody activity of the globulins. Albumin feeding and the birth weight of the pigs had no effect on survival. The absence of the birth weight effect, which had been observed consistently in earlier experiments, was probably due to the fact that it was not possible, in allotting the pigs, to establish an appreciable difference in average birth weight.Mortality increased progressively during the experiment, indicating an increasing disease level. It was concluded that improved sanitary practices would be essential if this type of rearing program were to have practical value.The distribution of serum proteins at 2 days of age reflected experimental treatments. In globulin-treated pigs the usual decline in γ-globulin levels between 2 days and 4–6 weeks of age was noted. Possible reasons for certain unexpected differences in serum albumin levels are discussed.

scholarly journals Serum protein electrophoretic pattern in piglets during the early postnatal period

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Csilla Tóthová ◽  
Robert Link ◽  
Petronela Kyzeková ◽  
Oskar Nagy
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Electrophoretic Pattern ◽  
Postnatal Period ◽  
Early Postnatal Period ◽  
Total Proteins ◽  
Newborn Piglets ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

AbstractThe pattern of serum proteins, the typical features of the electrophoretogram in newborn piglets and during their postnatal development is not completely described. Therefore, the aim of this study was to characterize the changes in serum protein electrophoretic pattern and features of the electrophoretograms during the early postnatal period. Significant changes during the monitored period were found in all evaluated parameters (P < 0.001). The most marked changes were observed mainly in the period before weaning. The concentrations of total proteins, albumin and γ-globulins were before colostrum intake low, γ-globulins represented the smallest proportion of protein fractions. The proportion of α1-globulins was after birth a dominant protein fraction. Significant increase of total proteins, α2-, β- and γ-globulins and decrease of α1-globulins was found 2 days after colostrum intake. The albumin and A/G values increased after birth gradually until weaning. After weaning a significant changes were found in absolute concentrations of total protein and albumin, and in relative values of β-globulin fractions. Presented results showed marked developmental alterations in the serum protein pattern in piglets along with the age. The study also brings new knowledge in the field of description of typical features of electrophoretograms in the observed period of piglet’s life.

Staining Properties of Oil Red O and a Method of Partial Purification of the Commercial Product

1959 ◽  
Vol 5 (2) ◽  
pp. 149-160 ◽  
Author(s):  
Henn Kutt ◽  
Theodore T Tsaltas
Keyword(s):  
Crude Oil ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Partial Purification ◽  
Paper Strip ◽  
Partial Removal ◽  
Lipid Pattern ◽  
Oil Red O ◽  
Human Serum Proteins ◽  
Commercial Oil

Abstract 1. A yellow-brown component of commercial oil red O separated by our technic of paper chromatography is shown to stain human serum proteins, particularly those that are coagulated by heat. The patterns of serum electropherograms obtained by coloring with the crude oil red O solution consist of a red lipid pattern superimposed on a brownish protein pattern. 2. A method is described for partial removal of the nonred components from the crude oil red O. Satisfactory coloration of lipoproteins was obtained with the "purified" oil red O. 3. It is demonstrated that areas of paper strip covered with protein may stain lighter with some oil red O fractions than the background filter paper.

scholarly journals Studies in Bisalbuminemia: Binding Properties of the Two Albumins

Blood ◽  
1962 ◽  
Vol 20 (2) ◽  
pp. 156-164 ◽  
Author(s):  
EDWARD J. SARCIONE ◽  
C. WILLIAM AUNGST
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Total Serum ◽  
Binding Properties ◽  
Normal Human ◽  
Serum Components

Abstract 1. An abnormal serum protein pattern in a patient with Wegener’s granulomatosis and five of his relatives was identified as bisalbuminemia by electrophoretic and immunochemical methods. 2. With the exception of the patient with Wegener’s syndrome, the presence of bisalbuminemia was not associated with a significant change in total serum proteins, total albumin, serum components other than albumin, or any disease. 3. Addition of I131-thyroxine to bisalbumin sera resulted in thyroxine binding by albumin B but not by albumin A. The failure of albumin A to bind added I131-thyroxine leads to speculation that, in this family, neither albumin A nor B are identical to normal human serum albumin.

Effect of Dietary Rapeseed Meal on the Serum Proteins of Rats

1971 ◽  
Vol 49 (12) ◽  
pp. 1100-1105 ◽  
Author(s):  
M. T. Lo ◽  
D. C. Hill
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Rapeseed Meal ◽  
Male Rats ◽  
Binding Properties ◽  
Binding Ability ◽  
Protein Sources ◽  
Altered Serum

Two experiments were conducted in which growing male rats were fed three diets containing as protein sources, casein (C), rapeseed meal variety Bronowski (BM), and B. napus meal (NM). In the first experiment the diets were fed to both thyroidectomized rats and to sham-operated rats with thyroid intact. Poorest gains were recorded for rats fed the NM diet irrespective of whether the thyroids were removed or intact, indicating that the toxicity of B. napus meal was not confined to the effect on the thyroid gland. In the second experiment four groups of rats were fed BM diet, NM diet, and C diet. The latter diet was given to both thyroidectomized rats and rats with intact thyroid to provide controls for the study of the effect on the thyroid of the glucosinolate in rapeseed. As in experiment 1, weight gain of rats receiving NM diet was less than that of either of the two groups of rats receiving C diet. After 25 days the serum protein and the thyroxine-binding properties of serum proteins were examined by disc gel electrophoresis. Rats fed NM or C diet (with thyroid removed) showed an altered serum protein pattern as compared to rats fed C or BM diets. The 131I-thyroxine-binding ability of the albumin band was much greater for the serum of rats receiving diets NM and C (with thyroid removed), 37% and 46% versus 22% of the total 131I-thyroxine recovered on the gel.

EFFECT OF TEMPERATURE AND PHOTOPERIOD ON THE SERUM PROTEINS OF THE RAINBOW TROUT, SALMO GAIRDNERI

1962 ◽  
Vol 40 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Herman M. Meisner ◽  
C. P. Hickman Jr.
Keyword(s):  
Rainbow Trout ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Paper Electrophoresis ◽  
Effect Of Temperature ◽  
Salmo Gairdneri

The serum of 1- and 2-year-old rainbow trout, acclimated at least 43 days to four conditions of temperature and daylength (16°–8L (hours daily photoperiod), 16°–16L, 8°–8L, and 8°–16L) was examined by paper electrophoresis. The albumin/globulin ratio was significantly greater in trout acclimated to 8 °C (pooled photoperiod) than in those acclimated to 16 °C (pooled photoperiod). Photoperiod had no significant effect on the serum protein pattern.

scholarly journals SITES OF FORMATION OF IMMUNE GLOBULINS AND OF A COMPONENT OF C'3

1961 ◽  
Vol 114 (4) ◽  
pp. 459-470 ◽  
Author(s):  
G. M. Hochwald ◽  
G. J. Thorbecke ◽  
R. Asofsky
Keyword(s):  
Amino Acids ◽  
Serum Protein ◽  
Liver Tissue ◽  
Serum Proteins ◽  
Mouse Serum ◽  
Spleen Tissue ◽  
Specificity And Sensitivity ◽  
Immune Globulins

The development of a new method for the determination of the sites of serum protein formation has been described. The method involves the incorporation of C14-labeled amino acids by tissues cultured in vitro, and subsequent autoradiography of immunoelectrophoretic patterns prepared from a mixture of culture fluids and carrier serum with an antiserum against the carrier serum. This technique has been used to demonstrate formation of γ-globulin, of ß2-macroglobulin, and of a component of C'3 by mouse spleen tissue, and of various other serum proteins by liver tissue. The specificity and sensitivity of this method have been discussed, and some of its advantages and pitfalls were mentioned. In addition, a rabbit antimouse serum was prepared, and the immunoelectrophoretic patterns obtained with mouse serum were compared with those described in the literature.

THE SERUM PROTEINS IN DIABETES MELLITUS IN RELATION TO VASCULAR COMPLICATIONS

1963 ◽  
Vol 43 (1) ◽  
pp. 3-11 ◽  
Author(s):  
Bengt Skanse
Keyword(s):  
Diabetes Mellitus ◽  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Vascular Complications ◽  
Electrophoretic Analysis ◽  
List Type ◽  
Globulin Fraction ◽  
Patients With Diabetes ◽  
Γ Globulins

ABSTRACT Electrophoretic analysis of the serum protein pattern in 110 patients with diabetes mellitus of varying duration and severity confirmed that the α2-globulins are often increased and that the albumin is often decreased. It was found that the γ-globulins may either be decreased or increased. When the β-globulins are increased, the increase is due to an increase of the β2-globulin fraction. The incidence of the protein changes is about equal in patients with and without vascular complications.

The content and composition of protein in creamery milks in south-west Scotland

1980 ◽  
Vol 47 (1) ◽  
pp. 83-90 ◽  
Author(s):  
D. Thomas Davies ◽  
Andrew J. R. Law
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Seasonal Pattern ◽  
Protein Composition ◽  
Experimental Period ◽  
Constant Composition ◽  
Milk Serum ◽  
South West ◽  
Total Milk ◽  
The Individual

SUMMAARYThe content and composition of protein in milk samples from creameries in south-west Scotland were determined over a period of 12 months. The composition of the whole casein was expressed in terms of αsl-, β-, κ-, αs2- and γ-caseins, and that of the total milk serum protein in terms of β-lactoglobulins (β-lg), α-lactalbumins, bovine serum albumin, and a mixture of immunoglobulins, proteose-peptone component 3 and lactoferrin (IPL). Concentrations of the individual caseins varied appreciably and for most, concentration was closely correlated with and showed the same seasonal pattern as total casein concentration. Concentrations of the milk serum proteins also varied but only those of β-lg and the IPL fraction were closely correlated with that of total milk serum protein and seasonal trends were not marked. Relative amounts of the individual proteins, on the other hand, showed smaller variations and so throughout the experimental period the milks contained a protein complex of comparatively constant composition. Because of this comparative constancy it would appear that seasonal variations in milk properties in south-west Scotland are unlikely to be determined to a major extent by milk protein composition, but could be more affected by protein concentration.

scholarly journals The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

2017 ◽  
Vol 67 (2) ◽  
pp. 178-190 ◽  
Author(s):  
Csilla Tóthová ◽  
Pavol Mudroň ◽  
Oskar Nagy
Keyword(s):  
Dairy Cows ◽  
Serum Protein ◽  
Serum Proteins ◽  
Inflammatory Diseases ◽  
Post Partum ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Total Serum ◽  
Control Group ◽  
Γ Globulins

Abstract The objective of the study was to evaluate the electrophoretic pattern of serum proteins in dairy cows suffering from various inflammatory diseases, and to study the influence of these diseases on the concentrations of protein fractions. Fourty dairy cows with clinical signs of various inflammatory diseases were used in the study, including cows with post-partum metritis (n=10), mastitis (n=7), and hoof diseases (n=23). The cows were of a black pied Holstein-Friesian breed, Slovak spotted breed and their crossbreeds at the age of 3.5 to 8 years. Fourteen clinically healthy dairy cows were taken as the control group. The cows were blood sampled for the determination of total serum proteins and serum protein fractions. The protein fractions were divided into albumin, α1-, α2-, β1-, β2-, and γ-globulins. In cows with post-partum metritis we found significantly lower concentrations of albumin (P<0.001) and significantly higher values of α1-globulins (P<0.01) compared with healthy animals. Significantly higher concentrations of β1-globulins were observed in cows with mastitis (P<0.001), while the γ-globulin fraction was non-significantly higher. In cows with hoof diseases we recorded significantly lower values of albumin (P<0.001), significantly higher concentrations of α1- and β1-globulins (P<0.001), and non-significantly higher β2- and γ-globulins. Moreover, in the electrophoretic pattern of serum proteins we found β-γ bridging in thirteen cows with hoof diseases. Presented data suggest marked influence of inflammatory diseases on the concentrations of serum protein fractions in dairy cows. However, further investigations are needed to establish the diagnostic utility of serum protein electrophoresis in bovine clinical practice in relation to inflammatory diseases.

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