In situ degradation of timothy conserved as restrictively or extensively fermented silage or as hay

2006 ◽  
Vol 86 (2) ◽  
pp. 299-306 ◽  
Author(s):  
R. Martineau ◽  
H. Lapierre ◽  
D. R. Ouellet ◽  
D. Pellerin ◽  
R. Berthiaume
Keyword(s):  
Formic Acid ◽  
Protein Degradation ◽  
Protein Fraction ◽  
Phleum Pratense ◽  
Neutral Detergent Fiber ◽  
In Situ Degradation ◽  
Forage Type ◽  
Phleum Pratense L ◽  
Degradable Protein

The objective of this study was to determine the effects of three modes of preserving timothy (Phleum pratense L.) on in situ degradation of dry matter (DM), organic matter, crude protein (CP), neutral detergent fiber and acid detergent fiber. First-cut timothy was conserved as hay, as restrictively fermented silage (formic acid 85% applied at 6 L t-1 of fresh crop), or as extensively fermented silage (inoculation with a mixture of Lactobacillus plantarum and Pediococcus cerevisiae yielding 1.25 × 1011 total CFU t-1). Six mid-lactating Holstein cows, previously adapted to the tested forage, were used to study the in situ degradation of each forage type (two cows per forage type). Triplicate bags were filled with 3.5 g DM equivalent of chopped wet forage and incubated in the rumen during 2, 4, 8, 12, 24, 48, or 72 h. Zero-hour bags were not inserted in the rumen. The conservation method affected in situ degradation of CP. Haying decreased (P ≤0.03) soluble protein (fraction a), degradation rate (c), lag time and effective degradability of CP in the rumen, and increased (P < 0.01) potentially degradable protein (fraction b) when compared with ensiling. Restricting fermentation in silage decreased (P < 0.01) fraction a and increased (P = 0.01) CP fraction b when compared with extensively fermented silage. Results indicate that conserving timothy as hay significantly decreases solubility, rate and extent of protein degradation in the rumen when compared with ensiling. Results also show that solubilization of protein in silage can be reduced by restricting fermentation with formic acid as compared with promoting fermentation with a bacterial inoculant, without a concurrent decrease in the extent of protein degradation in the rumen. Key words: In situ degradation, timothy, hay, restrictively fermented silage, extensively fermented silage, formic acid, inoculation

scholarly journals SYNCHRONIZATION OF CARBOHYDRATE AND PROTEIN SUPPLY IN THE SUGARCANE BAGASSE BASED RATION ON IN SITU NUTRIENT DEGRADABILITY

2016 ◽  
Vol 41 (1) ◽  
pp. 28-36 ◽  
Author(s):  
F. F. Sani ◽  
L. K. Nuswantara ◽  
E. Pangestu ◽  
F. Wahyono ◽  
J. Achmadi
Keyword(s):  
Sugarcane Bagasse ◽  
Crude Protein ◽  
Degradation Kinetics ◽  
Nitrogen Supply ◽  
Neutral Detergent Fiber ◽  
Synchronization Index ◽  
In Situ Degradation ◽  
Protein Supply ◽  
Kinetics Of

Two adult male sheeps fitted with rumen cannula were used in two experiments to study the effects of synchronization of carbohydrate and nitrogen supply in sugarcane bagasse based total mixed ration (TMR) on in situ nutrient degradation. The first experiment was aimed to create three TMR with different synchronization index. Ingredient feedstuffs of TMR were evaluated for its in situ organic matter (OM) and nitrogen (N) degradation kinetics. On the basis of the OM and N degradation kinetics of feedstuffs, three sugarcane bagasse based TMR were formulated with synchronization indexes of 0.37; 0.50; and 0.63; respectively. The TMR had similar levels of crude protein (CP), total digestible nutrients, and neutral detergent fiber (NDF). In the second experiment, the three TMR with different synchronization index were evaluated for in situ degradability characteristics of OM, CP, NDF, and sulfur. The in situ degradation of OM in TMR were decreased (P<0.05) with the increasing of synchronization index. The higher synchronization index in TMR increased (P<0.05) CP degradation of CP. The NDF degradation decreased slightly by the alteration of synchronization index in TMR. The higher synchronization index in TMR reduced (P<0.05) in situ sulfur degradation, and this may not support to effects of synchronization of carbohydrate and nitrogen supply.  

scholarly journals In situ caecal degradation of roughages in horses

2010 ◽  
Vol 39 (2) ◽  
pp. 349-355 ◽  
Author(s):  
Vinícius Pimentel Silva ◽  
Fernando Queiroz de Almeida ◽  
Eliane da Silva Morgado ◽  
Liziana Maria Rodrigues ◽  
Tiago Marques dos Santos ◽  
...  
Keyword(s):  
Cynodon Dactylon ◽  
Pigeon Pea ◽  
Neutral Detergent Fiber ◽  
Desmodium Ovalifolium ◽  
Randomized Design ◽  
Degradation Rates ◽  
Digestion Technique ◽  
In Situ Degradation ◽  
The Times

The present study was carried out to evaluate the in situ degradation of dry matter (DM), neutral detergent fiber (NDF) and crude protein (CP) in roughages by the in situ caecal digestion technique in horses. The roughages evaluated were: Lucerne hay (Medicago sativa), peanut (Arachis pintoi cv. Amarillo), desmodio(Desmodium ovalifolium), stylo (Stylosanthes guianensis cv. Mineirão), pigeon pea (Cajanus cajan), lime-yellow pea (Macrotyloma axillare) and coastcross hay (Cynodon dactylon cv. coastcross). The assay was conducted in a complete randomized design with seven roughages and three replications. One mare with a cannula fitted in the caecum was used, fed diet consisting of coastcross hay (80%) and concentrate (20%) at 2.0% BW, four times a day. Nylon 6.5 × 20 cm bags were used with 45 μ/pore, containing 5.2 g DM/bag, inserting 3 or 4 bags in the caecum at the times of 2, 4, 6, 8, 12, 24 and 48 hours incubation. The caecum in situ degradability parameters of nutrients were obtained by Ørskov model. The DM degradability parameters of all the roughages were significant. There was no fit to the model for pigeon pea for CP and NDF and desmodio. Peanut, stylo and lime-yellow pea presented larger potentially degradable DM with values of 53, 46.5 and 40%, respectively, and higher values for the soluble fraction of 20, 21, 28.6%, with high degradability rates of 10.36, 20.26 and 14.8% h-1. Higher NDF degradation rates were observed in these foodstuffs with values of 9.1 and 11.3, 11.2% h-1, high potentially degradable fraction with values of 55, 51.8 and 47.2%, and greater CP degradation at 48 hours with values of 87, 95, and 94.8%. Peanut, stylo and lime-yellow pea presented potential for use in horses diets.

Leaf and stem nutritive value of timothy genotypes under varying N fertilization

2004 ◽  
Vol 84 (4) ◽  
pp. 1101-1104 ◽  
Author(s):  
G. Bélanger ◽  
A. Brégard ◽  
R. Michaud ◽  
G. F. Tremblay
Keyword(s):  
Cell Wall ◽  
Nutritive Value ◽  
N Fertilization ◽  
Phleum Pratense ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
True Digestibility ◽  
Sib Families ◽  
Phleum Pratense L

Populations and half-sib families of timothy (Phleum pratense L.), contrasting in forage N concentration, were studied in two experiments conducted in growth rooms with varying N applications. They differed in leaf and stem in vitro true digestibility (IVTD) of DM and in vitro cell wall digestibility (IVCWD), and in stem neutral detergent fiber (NDF) concentration; this confirms the presence of genetic variability for leaf and stem nutritive value in timothy under both limiting and non-limiting N conditions. Key words: Digestibility, grasses, Phleum pratense, neutral detergent fiber, acid detergent fiber

scholarly journals The effect of bale density and addition of formic acid on the in situ dry matter and crude protein degradation of lucerne, red clover and red fescue silages

2014 ◽  
Vol 23 (2) ◽  
pp. 177-184 ◽  
Author(s):  
C. Purwin ◽  
M. Fijałkowska ◽  
B. Kowalik ◽  
H. Skórko-Sajko ◽  
Z. Nogalski ◽  
...  
Keyword(s):  
Formic Acid ◽  
Protein Degradation ◽  
Crude Protein ◽  
Dry Matter ◽  
Red Clover ◽  
Red Fescue

Leaf and stem nutritive value of timothy cultivars of different maturity at an irrigated site in southwestern Saskatchewan

2005 ◽  
Vol 85 (2) ◽  
pp. 377-383 ◽  
Author(s):  
Paul G. Jefferson
Keyword(s):  
Nutritive Value ◽  
P Uptake ◽  
Phleum Pratense ◽  
Forage Yield ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Days To Maturity ◽  
Fertilizer Rates ◽  
Phleum Pratense L ◽  
Or Modelling

Leaf and stem structural components contribute to variation in nutritive value among timothy (Phleum pratense L.) cultivars. Modelling timothy growth and nutritive value requires a better understanding of this variation. A trial of 11 timothy cultivars was conducted on irrigation at Swift Current, Saskatchewan from 1995 to 1997. Leaf:stem ratio, forage yield, days to maturity, N, P, acid detergent fiber (ADF) and neutral detergent fiber (NDF) concentrations, N/P ratio and P uptake of leaf and stem components were determined for spring growth forage. Two N fertilizer rates (100 and 150 kg N ha-1) did not consistently affect leaf and stem nutritive value. Cultivars differed in maturity, stem P, stem N, stem NDF, leaf NDF and leaf ADF in all 3 yr. Days to maturity was correlated to stem P concentration (r = 0.59, P < 0.05, to 0.77, P < 0.01) and leaf ADF concentration (r = 0.59, P < 0.05, to 0.82, P < 0.01) in all 3 yr but not to stem NDF concentration. Days to maturity was correlated to stem N concentration and leaf NDF concentration in 1995 and 1997 but not in 1996. However, days to maturity did not explain the observed variation among cultivars for stem NDF concentration and several other variables. While maturity differences among timothy cultivars must be taken into account when selecting for improved nutritive value or modelling growth and quality, additional variation for these traits was evident. Key words: Forage quality, Phleum pratense L., maturity, forage yield

Short Communication: Diurnal variations of nonstructural carbohydrates and nutritive value in timothy

2012 ◽  
Vol 92 (5) ◽  
pp. 883-887 ◽  
Author(s):  
Chantale Morin ◽  
Gilles Bélanger ◽  
Gaëtan F. Tremblay ◽  
Annick Bertrand ◽  
Yves Castonguay ◽  
...  
Keyword(s):  
Nutritive Value ◽  
Short Communication ◽  
Phleum Pratense ◽  
Diurnal Variations ◽  
Neutral Detergent Fiber ◽  
Day Length ◽  
Nonstructural Carbohydrates ◽  
Maximum Value ◽  
Summer Growth ◽  
Phleum Pratense L

Morin, C., Bélanger, G., Tremblay, G. F., Bertrand, A., Castonguay, Y., Drapeau, R., Michaud, R., Berthiaume, R. and Allard, G. 2012. Short Communication: Diurnal variations of nonstructural carbohydrates and nutritive value in timothy. Can. J. Plant Sci. 92: 883–887. Nonstructural carbohydrate (NSC) concentration in timothy (Phleum pratense L.) increased during the day by 16 to 41 g kg−1 DM (+23 to 137%) in spring and summer growth cycles and reached a maximum value between 12.8 and 13.2 h after sunrise in an area with 15 to 16 h of day length. This increase in NSC concentration was generally accompanied by a decrease in concentrations of neutral detergent fiber (−2 to 6%), acid detergent fiber (−1 to 7%), and N (−6 to 11%).

Delayed harvest affects mineral and NDF concentrations, and digestibility of timothy

2008 ◽  
Vol 88 (2) ◽  
pp. 325-329 ◽  
Author(s):  
S. Pelletier ◽  
G. F. Tremblay ◽  
G. Bélanger ◽  
P. Seguin ◽  
R. Drapeau ◽  
...  
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Metabolic Disorders ◽  
Nutritive Value ◽  
Phleum Pratense ◽  
Neutral Detergent Fiber ◽  
Fiber Concentration ◽  
Harvest Delay ◽  
Phleum Pratense L

A 1-wk delay after late heading in the harvest of timothy spring growth reduced dietary cation-anion difference (-17%), in vitro digestibilities of dry matter (-7%) and neutral detergent fiber (-10%), and increased neutral detergent fiber concentration (+5%). In summer regrowth, the harvest delay tended to have similar effects, but variations (1-6%) were smaller. Key words: Nutritive value, stages of development, Phleum pratense L., metabolic disorders

In situ rumen degradation kinetics of timothy and alfalfa as affected by cultivar and stage of maturity

2004 ◽  
Vol 84 (2) ◽  
pp. 255-263 ◽  
Author(s):  
P. Yu ◽  
D. A. Christensen ◽  
J. J. McKinnon
Keyword(s):  
Degradation Kinetics ◽  
Lag Time ◽  
Phleum Pratense ◽  
Neutral Detergent Fiber ◽  
Order Kinetic ◽  
Rumen Degradation ◽  
Degradation Pattern ◽  
Timothy 2 ◽  
Full Head

The objective of this study was to investigate the effects of cultivar and stage of maturity at cutting on rumen degradation characteristics of some feed components in alfalfa (Medicago sativa L.) and timothy (Phleum pratense L.). Two adapted cultivars of alfalfa (Pioneer and Beaver) and timothy (Climax and Joliette), grown in Saskatchewan (Canada), were cut at three stages: 1 = 1 wk before commercial cut (early bud for alfalfa; joint for timothy); 2 = at commercial cut (late bud for alfalfa; pre-bloom head for timothy); 3 = 1 wk after commercial cut (early bloom for alfalfa; full head for timothy). Rumen degradation characteristics, including rapidly degradable fraction (A), potentially degradable fraction (B), undegradable fraction (C), degradation rate (Kd), lag time (T0), and effective degradability (ED) of each component, were determined using in situ technique and analyzed by first order kinetic equation described by Ørskov and McDonald with lag time. The results showed that, in alfalfa, the cultivars have no effect on rumen degradation characteristics of dry matter (DM), organic matter (OM), crude protein (CP), and neutral detergent fiber (NDF) and acid detergent fiber (ADF). However, the stage of maturity at cutting has large effects on each component, except CP. Generally, with increasing stage of maturity, it decreased A or B and increased C without affecting Kd and T0, thus resulting in decreasing the ED fraction. In timothy, the cultivars have effects only on rumen degradation characteristics of CP. Climax has a higher effective degradability of CP (EDCP) than Joliette. The stage of maturity at cutting has a large impact on rumen degradation characteristics of each component. Increasing stage of maturity did not affect A, but reduced B and increased C without affecting Kd and T0, thus resulting in reducing ED fraction. In conclusion, Pioneer and Beaver alfalfa have similar rumen degradation characteristics for all feed components. Climax and Joliette timothy were different only on CP degradation pattern with Climax having a higher EDCP fraction than Joliette. The stage of maturity at cutting had a significant impact on rumen degradation characteristics for both forages. Generally, it decreased ED with increasing stage of maturity. Our results indicate that it is inappropriate to assume fixed degradation characteristics for a forage without considering factors such as stage of maturity at cutting. Key words: Chemical component, rumen degradation characteristics, forage, cultivar, maturity

scholarly journals Ruminal degradation of Panicum grasses in three post-regrowth ages

2020 ◽  
Vol 21 ◽  
Author(s):  
Bruno Spindola Garcez ◽  
Arnaud Azevêdo Alves ◽  
Ernando de Oliveira Macedo ◽  
Claudiane Morais dos Santos ◽  
Daniel Louçana da Costa Araújo ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Neutral Detergent Fiber ◽  
Composition Analysis ◽  
Acid Detergent Fiber ◽  
Randomized Design ◽  
Ruminal Degradation ◽  
In Situ Degradation ◽  
Completely Randomized Design

Abstract The objective was to evaluate the chemical composition and in situ degradation of Maasai, Mombasa and Tanzania grasses belonging to the genus Panicum, at 22, 34 and 46 days after regrowth. The contents of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were determined. For the evaluation of ruminal degradation, 4 g sample were placed in nylon bags and incubated in the rumen of a fistulated animal, for 6, 24 and 72 h. The experiment had a completely randomized design for chemical composition analysis and a split-split plot completely randomized design for in situ degradation, with means compared by Tukey test at 5%. There was an increase in the contents of DM (+ 1.69%) and NDF (+ 5.06%) (P <0.05) of the grasses at the highest cutting ages with reduction in the CP fraction. The potential degradation (PD) of DM, NDF and CP decreased with advancing age of grasses, with an increase in colonization time (0.69 h) and NDF degradation rate (1.14%/h). The increase in the post-regrowth age of the Massai, Mombasa, and Tanzania grasses increases the acid detergent fiber content and reduces the crude protein content, with a negative effect on the degradation of DM, PB and NDF. The management of these cultivars is indicated at 22 and 34 days post-regrowth to obtain forage with better nutritional value.

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