In vitro degradation of fresh substrates treated with exogenous fibrolytic enzymes

2002 ◽  
Vol 82 (4) ◽  
pp. 611-615 ◽  
Author(s):  
G. R. Bowman ◽  
K. A. Beauchemin ◽  
J. A. Shelford
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Degradation ◽  
Total Mixed Ration ◽  
Fibrolytic Enzymes ◽  
Enzyme Product ◽  
Dry Matter Degradation

An exogenous fibrolytic enzyme product was applied to three different proportions of a total mixed ration (TMR) and fed to dairy cows. There was no enhancement of the hydrolytic capacity of rumen fluid from cows serving as inoculum donors as a result of enzyme supplementation. In vitro dry matter degradation of the TMR at 12 h of incubation was increased by 15% when enzymes were added to the concentrate portion, and by 17% when added to the premix portion of the TMR (P < 0.05), but adding enzymes to the supplement (pelleted portion of the concentrate) had no effect. Key words: Enzymes, fresh substrate, dry matter degradation

The use of fresh or thawed rumen fluid containing glycerol or particle associated microbes to estimate in vitro degradation of feeds

2003 ◽  
Vol 2003 ◽  
pp. 149-149
Author(s):  
R. Mohamed ◽  
A. S. Chaudhry ◽  
P. Rowlinson
Keyword(s):  
Microbial Activity ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Rapeseed Meal ◽  
In Vitro Degradation ◽  
Dry Matter Degradation

Thawed rumen contents have been used to obtain strained rumen fluid (SRF) to estimate in vitro dry matter degradation (DMD) of feeds (Mohamed et al., 2002). However, thawed SRF (TSRF) gave lower DMD than fresh SRF (FSRF) which was partly attributed to reduced microbial activity in TRSF following storage at -20°C. This study examined the addition of glycerol (G) as cryopreservative and washing from particle associated microbes to SRF before its storage for later use as TSRF to estimate in vitro degradation of rapeseed meal (Rsd) and grass nut (Gnt).

Effects of source of rumen fluid on in vitro dry matter digestibility of feeds determined using the DAISYII incubator

2006 ◽  
Vol 86 (3) ◽  
pp. 439-441 ◽  
Author(s):  
J. King ◽  
J. C. Plaizier
Keyword(s):  
Key Words ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Ruminal Fluid ◽  
Total Mixed Ration ◽  
Inoculum Source ◽  
Dry Matter Digestibility ◽  
Percentage Points ◽  
Grass Hay

Apparent (ADD) and true (TDD) in vitro dry matter digestibilities of 12 ruminant feeds were determined with the DAISYII incubator (ANKOM Technology Corp. Macedon, NY) using inoculum prepared from ruminal fluid obtained from steers fed grass hay or from cows fed total mixed ration (56% forage and 44% grain, DM basis). Inoculum source did not affect ADD and TDD. Averaged across feeds and sources of inoculum, ADD was 6.7 percentage points lower than TDD. Key words: In vitro dry matter digestibility, ruminant feeds, inoculum, ANKOM DAISYII incubator

scholarly journals In vitro fermentation of corn silage using rumen fluid buffered or not and different sample amounts

2015 ◽  
Vol 45 (12) ◽  
pp. 2229-2232
Author(s):  
Antonio Carlos Homem Junior ◽  
Jane Maria Bertocco Ezequiel ◽  
Henrique Leal Perez ◽  
Marco Tulio Costa Almeida ◽  
Josimari Regina Paschoaloto ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Corn Silage ◽  
Buffer Solution ◽  
In Vitro Fermentation ◽  
Ch4 Production ◽  
Dry Matter Degradation ◽  
Sample Amount ◽  
Gas Volume

Methodological variations in the amount of sample incubated and the type of rumen fluid used are commonly observed. This study evaluated the effect of three sample amounts (0.6, 1.3 or 2.6g DM 100mL-1 of rumen fluid) incubated in rumen fluid buffered (BRF-buffered rumen fluid) or not (PRF-pure rumen fluid) on total gas volume (TV), methane (CH4) production, dry matter degradation (DM Deg) and final pH of corn silage incubated in vitro. The highest DM Deg was reached with the lowest amounts of sample (0.6 and 1.3g of DM 100mL-1 of rumen fluid). The sample amount of 2.6g associated with PRF reduced CH4 production (P<0.05), compared with the amounts of 0.6 and 1.3g DM 100mL-1, which had similar CH4 production (P>0.05). The use of BRF caused no effect on CH4 production (P>0.05), independent of the sample amount. Increasing the amount of substrate resulted in lower final pH of incubation in both fluids (P<0.05). Our results indicate that incubations should be performed with the smallest amount of sample (0.6g of DM 100mL-1 of rumen fluid), using fluid without buffer. Incubation without buffer solution overestimates the CH4 production of corn silage. Further studies should be conducted to verify the possibility of in vitro ruminal incubation of other ingredients using pure rumen fluid.

scholarly journals Utilization of canola seed fractions for ruminants: effect of canola fibre-protein and can-sugar inclusion in dehydrated alfalfa pellets on palatability and lactation performance of dairy cows

2010 ◽  
Vol 90 (2) ◽  
pp. 279-283 ◽  
Author(s):  
R G Heendeniya ◽  
D A Christensen ◽  
D D Maenz ◽  
J J McKinnon ◽  
P. Yu
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Dry Matter ◽  
Dry Matter Intake ◽  
Canola Seed ◽  
Lactation Performance ◽  
Total Mixed Ration ◽  
Dry Matter Digestibility ◽  
Sugar Mixture

This research evaluated the effect of fibre-protein and can-sugar additions to dehydrated alfalfa pellets on feed acceptance, lactation performance, dry matter intake and apparent dry matter digestibility of dairy cows compared with a standard (pure) alfalfa pellet. The results showed that a two:one ratio of fibre-protein to can-sugar mixture can be added at 150 g kg-1 to a standard alfalfa pellet without affecting the palatability and lactation performance of dairy cows, when the alfalfa pellets are included at 50 g kg-1 (as fed basis) of a total mixed ration (TMR). Key words: Canola fractions, fibre-protein, can-sugar, palatability, lactation performances, dairy cows

scholarly journals  Fresh or frozen rumen contents from slaughtered cattle to estimatein vitro degradation of two contrasting feeds

2012 ◽  
Vol 57 (No. 6) ◽  
pp. 265-273 ◽  
Author(s):  
A.S. Chaudhry ◽  
R.A.I. Mohamed
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Rapeseed Meal ◽  
Factorial Experiment ◽  
In Vitro Degradation

The present factorial experiment tested the thawed rumen fluid from frozen rumen contents against fresh rumen fluid from the same slaughtered cattle to estimate in vitro degradation of rapeseed meal (RSM) and grass nuts (GN) as two contrasting feeds at various times. Fresh rumen fluid showed higher (P &lt; 0.05) in vitro dry matter (DMD) and crude protein (CPD) degradation than the thawed rumen fluid at most incubation times. Significant differences (P &lt; 0.05) were found between fresh and differently prepared thawed rumen fluids for mean degradation constants (a, b, c) and effective degradability (P<sub>0.02</sub>) of feeds. While the thawed rumen fluids had lower (P &lt; 0.01) degradation than the fresh rumen fluid, their degradation values correlated very well (R<sup>2 </sup>&ge; 0.95) with those for the fresh rumen fluid. It appeared that the thawed rumen fluid can be used to predict in vitro degradation of the selected feeds when frequent access to slaughtered or fistulated animals is restricted. Nevertheless it would be vital to improve the activity of thawed rumen fluid prior to testing its suitability for numerous feeds before recommending its routine use to estimate degradation of ruminant feeds. &nbsp;

Effect of subacute ruminal acidosis on in situ digestion of mixed hay in lactating dairy cows

2001 ◽  
Vol 81 (3) ◽  
pp. 421-423 ◽  
Author(s):  
J. C. Plaizier ◽  
J. E. Keunen ◽  
J-P. Walton ◽  
T. F. Duffield ◽  
B. W. McBride
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Dry Matter ◽  
Subacute Ruminal Acidosis ◽  
Total Mixed Ration ◽  
Neutral Detergent Fibre ◽  
Ruminal Acidosis ◽  
Lactating Dairy Cows

Induction of subacute ruminal acidosis (SARA) by replacing 25% of the total mixed ration (TMR) intake [dry matter (DM) basis] with pellets consisting of 50% wheat and 50% barley, reduced the in situ DM and neutral detergent fibre (NDF) digestion of mixed hay. In situ 48 h NDF disappearance was reduced from 39.5 to 30.9% (P < 0.05). Key words: Sub-acute ruminal acidosis, fibre digestibilty, dairy cows

Digestibility of Nutrients as Influenced by Supplementation of Exogenous Fibrolytic Enzymes in Dry Non-pregnant Cows

2019 ◽  
Vol 14 (04) ◽  
Author(s):  
P. M, Lunagariya ◽  
R. S. Gupta ◽  
S. V. Shah ◽  
Y. G. Patel
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dairy Cows ◽  
Dry Matter ◽  
Body Weight Gain ◽  
The Body ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
Fibrolytic Enzymes ◽  
Nitrogen Free Extract

The study was planned to evaluate the effect of exogenous fibrolytic enzymes (EFE) supplementation for 56 days @ 240 mg/kg total mixed ration (TMR) on digestibility of dry matter and nutrients in dairy cows. Six dry non-pregnant cows were assigned in each treatment with and without EFE. The digestibility trial of seven days was conducted after 49 days of feeding. Dry matter and nutrients intake of cows was not influenced by EFE. The supplementation of EFE had improved digestibility of dry matter, organic matter, crude fiber, neutral detergent fiber, cellulose (p less than 0.01), as well as digestibility of nitrogen-free extract and acid detergent fiber, was also higher (pless than 0.05). The body weight gain of cows was higher on the supplementation of EFE in TMR. The study concluded that feeding exogenous fibrolytic enzymes (240 mg/kg) supplemented TMR improved digestibility of dry matter and nutrients, which was reflected as higher body weight gain in dry non-pregnant Gir and crossbred dairy cows.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

scholarly journals DAY-TO-DAY VARIATIONS IN FAT PERCENT OF COW'S MILK

1990 ◽  
Vol 70 (2) ◽  
pp. 731-734 ◽  
Author(s):  
A. S. ATWAL ◽  
J. D. ERFLE
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Milk Yield ◽  
Milk Fat ◽  
Rumen Fluid ◽  
Supplemental Feeding ◽  
Fat Percentage ◽  
Milk Fat Depression ◽  
Daily Milk Yield ◽  
Daily Milk

Large day-to-day variations in milk fat, particularly for the morning milkings, were observed in 36 Holstein cows. Changes in percent fat were gradual and produced wavelike patterns in a number of instances. Supplemental feeding of long hay had no effect on acetate/propionate ratio in rumen fluid, daily milk yield or weighted milk fat percentage. Key words: Dairy cows, milk, fat depression, hay

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