scholarly journals Use of fine needle aspirate from peripheral nerves of pure-neural leprosy for cytology and polymerase chain reaction to confirm the diagnosis: A follow-up study of 4 years

2017 ◽  
Vol 62 (6) ◽  
pp. 527
Author(s):  
Abhishek De ◽  
AbuHena Hasanoor Reja ◽  
Ishad Aggarwal ◽  
Sumit Sen ◽  
Amrita Sil ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Peripheral Nerves ◽  
Fine Needle Aspirate ◽  
Chain Reaction ◽  
Fine Needle ◽  
Follow Up Study ◽  
Polymerase Chain

scholarly journals Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study

2019 ◽  
Vol 13 (3) ◽  
pp. e0007147 ◽  
Author(s):  
Raquel R. Barbieri ◽  
Fernanda S. N. Manta ◽  
Suelen J. M. Moreira ◽  
Anna M. Sales ◽  
José A. C. Nery ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Polymerase Chain

scholarly journals Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study.

1991 ◽  
Vol 29 (1) ◽  
pp. 42-45 ◽  
Author(s):  
H C Claas ◽  
J H Wagenvoort ◽  
H G Niesters ◽  
T T Tio ◽  
J H Van Rijsoort-Vos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Polymerase Chain

Prevalence and persistence of asymptomatic Chlamydia trachomatis infections in urine specimens from Danish male military recruits.

2002 ◽  
Vol 13 (1_suppl) ◽  
pp. 19-22 ◽  
Author(s):  
Adriaan J C Van Den Brule ◽  
Christian Munk ◽  
Jeanette F Winther ◽  
Susanne Krüger Kjaer ◽  
Hans O Jørgensen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Clinical Symptoms ◽  
Sexual Partners ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Military Recruits ◽  
Polymerase Chain ◽  
Urine Specimens

Danish male military recruits (n = 388) were included in a follow-up study to investigate the prevalence and persistence of asymptomatic Chlamydia trachomatis infections. Urine specimens were collected at enrolment and after approximately six months. C. trachomatis was detected by polymerase chain reaction (Amplicor, Roche). Questionnaires were filled out concerning sexual behaviour and clinical symptoms. The prevalence of asymptomatic C. trachomatis in Danish male military recruits was 4.6% (18 out of 388). From five C. trachomatis-positive men no follow-up sample was obtained. From the remaining 13 C. trachomatis-positive men four (31%) were treated for C. trachomatis between the two visits (outside the study protocol). Of the remaining nine men, one cleared the infection and eight men (89%) had a persistent infection. The number of lifetime sexual partners was associated with the presence of C. trachomatis at enrolment. Although based on small numbers, this follow-up study shows, in contrast to women with asymptomatic C. trachomatis infections, a high percentage of C. trachomatis persistence in asymptomatically infected males.

BRAF Mutations in Melanocytic Lesions and Papillary Thyroid Carcinoma Samples Identified Using Melting Curve Analysis of Polymerase Chain Reaction Products

2007 ◽  
Vol 131 (9) ◽  
pp. 1361-1367
Author(s):  
Robert Hay ◽  
Erin MacRae ◽  
Duane Barber ◽  
Moosa Khalil ◽  
Douglas J. Demetrick
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Conventional Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Fine Needle Aspirate ◽  
Chain Reaction ◽  
Braf Mutations ◽  
Fine Needle ◽  
Melanocytic Lesions ◽  
Polymerase Chain

Abstract Context.—Mutations of the proto-oncogene B-raf (BRAF) have been detected in melanocytic lesions and papillary carcinomas of the thyroid, and identification of these mutations could be useful in resolving some diagnostic problems. Objective.—To develop a method to evaluate mutations of BRAF that could provide results much more rapidly than conventional polymerase chain reaction and DNA sequencing assays. Design.—An assay using a LightCycler was developed to evaluate DNA sequences encoding amino acids within the activation loop of BRAF. Results.—Using this real-time polymerase chain reaction method, we analyzed 55 paraffin-embedded melanoma or nevus samples. The V600E mutation was found in 0 (0%) of 13 samples diagnosed histologically as Spitz nevi, 9 (24.3%) of 37 invasive melanomas, and 5 (100%) of 5 other melanocytic nevi. Two additional mutations, V600K and VK600-1E, also were identified in cases of invasive melanoma. We analyzed 14 paraffin-embedded papillary thyroid cancer (PTC) samples, 6 of which showed the V600E mutation. We found that our test worked efficiently with fine-needle aspirate specimens, and it identified 6 V600E mutations in 10 fine-needle aspirate specimens diagnosed as PTC. We also identified 4 V600E mutations in 6 specimens of PTC metastatic to lymph node. Unlike the melanocytic lesions, the PTC specimens yielded only V600E mutations. Comparison of our real-time polymerase chain reaction results with conventional polymerase chain reaction and DNA sequencing demonstrated 100% concordance. Surprisingly, we did not identify the previously reported VK600-1E or K601E mutations in our PTC specimens. Conclusions.—Our results show that the real-time polymerase chain reaction method is a rapid and accurate method for identifying BRAF mutations, such as V600E, in both paraffin-embedded tissue and fine-needle aspirate specimens.

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