Quantitative reverse transcription polymerase chain reaction-based detection of thyroid-specific gene expression in fine-needle aspirate for thyroid cancer recurrence evaluation: A case report and review of the literature

Head & Neck ◽  
2015 ◽  
Vol 37 (11) ◽  
pp. E165-E168
Author(s):  
Saïd C. Azoury ◽  
Rachel Aufforth ◽  
Mei He ◽  
Zhiming Yang ◽  
Naris Nilubol ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Cancer Recurrence ◽  
Specific Gene ◽  
Review Of The Literature ◽  
Fine Needle Aspirate ◽  
Chain Reaction ◽  
Fine Needle ◽  
Polymerase Chain ◽  
Thyroid Cancer Recurrence

BRAF Mutations in Melanocytic Lesions and Papillary Thyroid Carcinoma Samples Identified Using Melting Curve Analysis of Polymerase Chain Reaction Products

2007 ◽  
Vol 131 (9) ◽  
pp. 1361-1367
Author(s):  
Robert Hay ◽  
Erin MacRae ◽  
Duane Barber ◽  
Moosa Khalil ◽  
Douglas J. Demetrick
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Conventional Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Method ◽  
Fine Needle Aspirate ◽  
Chain Reaction ◽  
Braf Mutations ◽  
Fine Needle ◽  
Melanocytic Lesions ◽  
Polymerase Chain

Abstract Context.—Mutations of the proto-oncogene B-raf (BRAF) have been detected in melanocytic lesions and papillary carcinomas of the thyroid, and identification of these mutations could be useful in resolving some diagnostic problems. Objective.—To develop a method to evaluate mutations of BRAF that could provide results much more rapidly than conventional polymerase chain reaction and DNA sequencing assays. Design.—An assay using a LightCycler was developed to evaluate DNA sequences encoding amino acids within the activation loop of BRAF. Results.—Using this real-time polymerase chain reaction method, we analyzed 55 paraffin-embedded melanoma or nevus samples. The V600E mutation was found in 0 (0%) of 13 samples diagnosed histologically as Spitz nevi, 9 (24.3%) of 37 invasive melanomas, and 5 (100%) of 5 other melanocytic nevi. Two additional mutations, V600K and VK600-1E, also were identified in cases of invasive melanoma. We analyzed 14 paraffin-embedded papillary thyroid cancer (PTC) samples, 6 of which showed the V600E mutation. We found that our test worked efficiently with fine-needle aspirate specimens, and it identified 6 V600E mutations in 10 fine-needle aspirate specimens diagnosed as PTC. We also identified 4 V600E mutations in 6 specimens of PTC metastatic to lymph node. Unlike the melanocytic lesions, the PTC specimens yielded only V600E mutations. Comparison of our real-time polymerase chain reaction results with conventional polymerase chain reaction and DNA sequencing demonstrated 100% concordance. Surprisingly, we did not identify the previously reported VK600-1E or K601E mutations in our PTC specimens. Conclusions.—Our results show that the real-time polymerase chain reaction method is a rapid and accurate method for identifying BRAF mutations, such as V600E, in both paraffin-embedded tissue and fine-needle aspirate specimens.

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document