Serial Measurements of Hepatitis C Viral Load by Real-Time Polymerase Chain Reaction Among Recipients of Living-Donor Renal Transplants: A Short-Term Follow-Up Study From a Single Center

2010 ◽  
Vol 42 (9) ◽  
pp. 3568-3573 ◽  
Author(s):  
S. Justa ◽  
R.W. Minz ◽  
M. Minz ◽  
A. Sharma ◽  
S. Anand ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Load ◽  
Living Donor ◽  
Single Center ◽  
Chain Reaction ◽  
Short Term ◽  
Follow Up Study ◽  
Polymerase Chain ◽  
Serial Measurements

scholarly journals Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study

2019 ◽  
Vol 13 (3) ◽  
pp. e0007147 ◽  
Author(s):  
Raquel R. Barbieri ◽  
Fernanda S. N. Manta ◽  
Suelen J. M. Moreira ◽  
Anna M. Sales ◽  
José A. C. Nery ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Polymerase Chain

scholarly journals Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study.

1991 ◽  
Vol 29 (1) ◽  
pp. 42-45 ◽  
Author(s):  
H C Claas ◽  
J H Wagenvoort ◽  
H G Niesters ◽  
T T Tio ◽  
J H Van Rijsoort-Vos ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Polymerase Chain

Prevalence and persistence of asymptomatic Chlamydia trachomatis infections in urine specimens from Danish male military recruits.

2002 ◽  
Vol 13 (1_suppl) ◽  
pp. 19-22 ◽  
Author(s):  
Adriaan J C Van Den Brule ◽  
Christian Munk ◽  
Jeanette F Winther ◽  
Susanne Krüger Kjaer ◽  
Hans O Jørgensen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Clinical Symptoms ◽  
Sexual Partners ◽  
Chain Reaction ◽  
Follow Up Study ◽  
Military Recruits ◽  
Polymerase Chain ◽  
Urine Specimens

Danish male military recruits (n = 388) were included in a follow-up study to investigate the prevalence and persistence of asymptomatic Chlamydia trachomatis infections. Urine specimens were collected at enrolment and after approximately six months. C. trachomatis was detected by polymerase chain reaction (Amplicor, Roche). Questionnaires were filled out concerning sexual behaviour and clinical symptoms. The prevalence of asymptomatic C. trachomatis in Danish male military recruits was 4.6% (18 out of 388). From five C. trachomatis-positive men no follow-up sample was obtained. From the remaining 13 C. trachomatis-positive men four (31%) were treated for C. trachomatis between the two visits (outside the study protocol). Of the remaining nine men, one cleared the infection and eight men (89%) had a persistent infection. The number of lifetime sexual partners was associated with the presence of C. trachomatis at enrolment. Although based on small numbers, this follow-up study shows, in contrast to women with asymptomatic C. trachomatis infections, a high percentage of C. trachomatis persistence in asymptomatically infected males.

scholarly journals Performance of the Innova SARS-CoV-2 antigen rapid lateral flow test in the Liverpool asymptomatic testing pilot: population based cohort study

BMJ ◽  
2021 ◽  
pp. n1637 ◽  
Author(s):  
Marta García-Fiñana ◽  
David M Hughes ◽  
Christopher P Cheyne ◽  
Girvan Burnside ◽  
Mark Stockbridge ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Load ◽  
Predictive Value ◽  
High Viral Load ◽  
Test Results ◽  
Chain Reaction ◽  
Viral Loads ◽  
Flow Test ◽  
Polymerase Chain ◽  
Lateral Flow Test

Abstract Objective To assess the performance of the SARS-CoV-2 antigen rapid lateral flow test (LFT) versus polymerase chain reaction testing in the asymptomatic general population attending testing centres. Design Observational cohort study. Setting Community LFT pilot at covid-19 testing sites in Liverpool, UK. Participants 5869 asymptomatic adults (≥18 years) voluntarily attending one of 48 testing sites during 6-29 November 2020. Interventions Participants were tested using both an Innova LFT and a quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) test based on supervised self-administered swabbing at testing sites. Main outcome measures Sensitivity, specificity, and predictive values of LFT compared with RT-qPCR in an epidemic steady state of covid-19 among adults with no classic symptoms of the disease. Results Of 5869 test results, 22 (0.4%) LFT results and 343 (5.8%) RT-qPCR results were void (that is, when the control line fails to appear within 30 minutes). Excluding the void results, the LFT versus RT-qPCR showed a sensitivity of 40.0% (95% confidence interval 28.5% to 52.4%; 28/70), specificity of 99.9% (99.8% to 99.99%; 5431/5434), positive predictive value of 90.3% (74.2% to 98.0%; 28/31), and negative predictive value of 99.2% (99.0% to 99.4%; 5431/5473). When the void samples were assumed to be negative, a sensitivity was observed for LFT of 37.8% (26.8% to 49.9%; 28/74), specificity of 99.6% (99.4% to 99.8%; 5431/5452), positive predictive value of 84.8% (68.1% to 94.9%; 28/33), and negative predictive value of 93.4% (92.7% to 94.0%; 5431/5814). The sensitivity in participants with an RT-qPCR cycle threshold (Ct) of <18.3 (approximate viral loads >10 6 RNA copies/mL) was 90.9% (58.7% to 99.8%; 10/11), a Ct of <24.4 (>10 4 RNA copies/mL) was 69.4% (51.9% to 83.7%; 25/36), and a Ct of >24.4 (<10 4 RNA copies/mL) was 9.7% (1.9% to 23.7%; 3/34). LFT is likely to detect at least three fifths and at most 998 in every 1000 people with a positive RT-qPCR test result with high viral load. Conclusions The Innova LFT can be useful for identifying infections among adults who report no symptoms of covid-19, particularly those with high viral load who are more likely to infect others. The number of asymptomatic adults with lower Ct (indicating higher viral load) missed by LFT, although small, should be considered when using single LFT in high consequence settings. Clear and accurate communication with the public about how to interpret test results is important, given the chance of missing some cases, even at high viral loads. Further research is needed to understand how infectiousness is reflected in the viral antigen shedding detected by LFT versus the viral loads approximated by RT-qPCR.

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