scholarly journals Detection of Arcobacter spp. in Mytilus galloprovincialis samples collected from Apulia region

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Elisabetta Bonerba ◽  
Anna Mottola ◽  
Antonio Parisi ◽  
Angela Di Pinto ◽  
Andrea Serraino ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Disease Outbreak ◽  
16S Rdna Sequencing ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Apulia Region ◽  
Rdna Sequencing ◽  
Arcobacter Butzleri ◽  
Polymerase Chain

The aim of the study was to evaluate the occurrence of <em>Arcobacter</em> spp. in 20 samples of <em>Mytilus galloprovincialis</em> purchased at fish markets in Apulia region. The detection of <em>Arcobacter</em> spp. was performed, after selective enrichment, on modified charcoal cefoperazone deoxycholate (mCCD) agar supplemented with Cefoperazone, Amphotericin B and Teicoplanin (CAT). In 6 out of the 20 tested samples the presence of <em>Arcobacter</em> spp. was found and confirmed by genus-based polymerase chain reaction. All the isolates were identified as belonging to the species<em> Arcobacter butzleri</em> using 16S rDNA sequencing and BLAST online. The results represent the first report in Italy of <em>A. butzleri</em> detection in marketed <em>Mytilus galloprovincialis</em>. The survey underlines the epidemiological importance of <em>A. butzleri</em> as an emerging pathogen, and highlights that mussels should be considered as a potential cause of foodborne disease outbreak.

Sensitivity of Three Methods Used in the Isolation of Arcobacter spp. in Raw Ground Pork

2002 ◽  
Vol 65 (11) ◽  
pp. 1784-1788 ◽  
Author(s):  
DAWN S. OHLENDORF ◽  
ELSA A. MURANO
Keyword(s):  
Buffer System ◽  
Isolation Method ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Gram Staining ◽  
Ground Pork ◽  
Isolation Methods ◽  
Arcobacter Butzleri ◽  
Polymerase Chain ◽  
Whole Muscle

Arcobacter, an aerotolerant Campylobacter-like organism, has been designated an emerging pathogen because of its newly recognized ability to cause diarrheal illness in both humans and animals and its presence in the human food supply. Because there is no standard isolation method for its detection, the true occurrence of this pathogen is largely unknown. In addition, the lack of a standardized isolation protocol limits the ability of investigators to compare field data. Arcobacter has been detected in whole muscle and ground pork at various levels by two different isolation methods (those of deBoer and Collins). In this study, these methods were tested along with the Johnson-Murano (JM) method, developed in our laboratory. The sensitivity of each method was tested for ground pork inoculated with Arcobacter butzleri and Arcobacter cryaerophilus 1A at levels of 104, 103, 102, and 101 CFU/g. Controls included tubes with uninoculated pork and broth tubes without pork. All samples that were morphologically similar to Arcobacter were analyzed by Gram staining and by catalase and oxidase reactions. Presumptive positive samples were confirmed by the polymerase chain reaction. The JM method was determined to be the most sensitive, detecting A. butzleri down to a level of 101 CFU/g in 100% of the samples and detecting A. cryaerophilus 1A at a level of 101 CFU/g in 75% of samples. In a pure buffer system, the Collins method was as effective as the JM method in isolating both organisms to levels of 101 cells per g.

scholarly journals Presence of Arcobacter species in pet cats and dogs in the Czech Republic

2017 ◽  
Vol 61 (No. 8) ◽  
pp. 449-455
Author(s):  
M. Pejchalova ◽  
S. Zabcikova ◽  
L. Silhova ◽  
D. Silha ◽  
I. Brozkova ◽  
...  
Keyword(s):  
Czech Republic ◽  
Gel Electrophoresis ◽  
Dna Isolation ◽  
Human Infection ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Two Samples ◽  
Arcobacter Butzleri ◽  
Polymerase Chain

This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat. In all five positive cases, PCR confirmed the presence of Arcobacter butzleri. In follow-up sampling, the presence of Arcobacter butzleri was demonstrated in two samples from a dog.

Comparison of EB and Fraser Enrichment Broths for the Detection of Listeria spp. and Listeria monocytogenes in Raw-Milk Dairy Products and Environmental Samples

1996 ◽  
Vol 59 (11) ◽  
pp. 1172-1175 ◽  
Author(s):  
GEERTRUI M. VLAEMYNCK ◽  
RENAAT MOERMANS
Keyword(s):  
Listeria Monocytogenes ◽  
Dairy Products ◽  
Raw Milk ◽  
Chain Reaction ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Isolation Frequency ◽  
Detection And Identification ◽  
Significant Difference ◽  
Polymerase Chain

This study is a comparison of the isolation frequency of Listeria spp. and Listeria monocytogenes from selected naturally contaminated dairy products, especially soft smear-ripened cheeses from raw milk and samples of feces and rinse samples from the udder taken on the farm, by using an enrichment broth (EB) recommended by the International Dairy Federation and the U.S. Food and Drug Administration (IDF and FDA) or Fraser broth as the selective enrichment. Detection and identification were carried out according to the IDF protocols and a polymerase chain reaction technique. Listeria spp. were detected in 39.8% of the 570 samples while 15.3% were positive for L. monocytogenes. For cheese and curd samples, Fraser enrichment broth gave a statistically significant higher recovery for all Listeria spp. (26 to 21 %) as well as for L. monocytogenes in particular (9 to 1.4%). For raw milk and samples taken from feces and the udder rinse no significant difference was found between EB and Fraser broth. A combination of both enrichments resulted in an increase of recovery over all matrices by 15%.

scholarly journals Recovery of Salmonella from biofilms in a headwater spring ecosystem

2011 ◽  
Vol 9 (3) ◽  
pp. 458-466 ◽  
Author(s):  
James P. Gaertner ◽  
Joseph A. Mendoza ◽  
Michael R. J. Forstner ◽  
Dittmar Hahn
Keyword(s):  
Pathogenic Bacteria ◽  
Chain Reaction ◽  
Selective Enrichment ◽  
Animal Wastes ◽  
San Marcos ◽  
Polymerase Chain ◽  
Spring Lake ◽  
Precipitation Events

Salmonellae are pathogenic bacteria often detected in waters impacted by human or animal wastes. In order to assess the fate of salmonellae in supposedly pristine environments, water and natural biofilm samples along with snails (Tarebia granifera) and crayfish (Procambarus clarkia) were collected before and up to 7 days following four precipitation events from sites within the headwater springs of Spring Lake, San Marcos, TX. The samples were analyzed for the presence of salmonellae by polymerase chain reaction (PCR) after semi-selective enrichment. Salmonellae were detected in one water sample directly after precipitation only, while detection in ten biofilm and two crayfish samples was not related to precipitation. Salmonellae were not detected in snails. Characterization of isolates by rep-PCR revealed shared profiles in water and biofilm samples, biofilm and crayfish samples, and biofilm samples collected 23 days apart. These results suggest that salmonellae are infrequently washed into this aquatic ecosystem during precipitation runoff and can potentially take up residency in biofilms which can help facilitate subsequent long-term persistence and eventual transfer through the food chain.

scholarly journals Aeromonas spp.: an emerging pathogen?

2015 ◽  
Vol 30 (2) ◽  
Author(s):  
Andrea Bartolini ◽  
Giulia Zorzi ◽  
Elena Castellani ◽  
Valeria Besutti
Keyword(s):  
Polymerase Chain Reaction ◽  
Abdominal Pain ◽  
Antibiotic Therapy ◽  
Microscopic Examination ◽  
Emerging Pathogen ◽  
Chain Reaction ◽  
Etiologic Role ◽  
Polymerase Chain ◽  
Stool Cultures

The aim of this study is to identify and monitor the presence of <em>Aeromonas</em> spp. strains in stool cultures. We analyzed 5564 stool cultures from September 2012 to August 2013. Sixty-three patients were positive for Aeromonas spp. The most frequent symptoms were: diarrhea (46.0%) and abdominal pain (12.7%). Pediatric subjects were 28. Samples’ microscopic examination showed leukocytes in 38.1% of cases. It is still controversial whether Aeromonas are responsible for human gastroenteritis, but their presence in faecies of symptomatic patients supports their etiologic role. We propose search for toxins by polymerase chain reaction to identify strains that require an antibiotic therapy.

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