Association of Activation of Induced COX-2, iNOS and Cytokines with NF-kappa B Depression by Taiwan Wild Grape Ethanolic Extract in Mice

2017 ◽  
Vol 60 (4) ◽  
pp. 242-252 ◽  
Author(s):  
Ching-Wen Chang
Keyword(s):  
Ethanolic Extract ◽  
Nf Kappa B ◽  
Wild Grape ◽  
Cox 2

scholarly journals Ekstrak Etanol Kulit Buah Naga Menurunkan Indikasi Neoplasia Mammae Tikus Putih Berdasarkan Histopatologi dan Inhibitor Siklooksigenase-2

2018 ◽  
Vol 19 (1) ◽  
pp. 23
Author(s):  
Muhammad Thohawi Elziyad Purnama ◽  
Ragil Angga Prastiya ◽  
Faisal Fikri ◽  
Amung Logam Saputro ◽  
Bodhi Agustono
Keyword(s):  
Mammary Gland ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Growth Function ◽  
Histopathological Features ◽  
White Rats ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Cox 2 ◽  
Dermal Injection

Cancer caused uncontrolled cell proliferation and triggered by changes on cell information that regulate growth function. Each cell has different potential so many cancer prevalence such as endometrial cancer, lymph node cancer, lung cancer, skin and mammary gland cancer. The aims of this study were to assess the potential of dragon fruit skin ethanolic extract on white rats (Rattus norvegicus) exposed 7,12- Dimethylbenz(á)antrasena (DMBA) on mammary gland based on histopathological features and cyclooxygenase-2 (Cox-2) intensity. This study were used 20 of rats randomly divided into five group and each groups consisted of four rats, i.e: K+ weren’t treated DMBA and extract; K- were treated with DMBA; P1 were treated with DMBA and extract 10 mg/kg BW; P2 were treated with DMBA and extract 15 mg/kg BW; P3 were treated with DMBA and extract 20 mg/kg BW. The DMBA was given by intra dermal injection during twice a week for five weeks and the extracts with gastric tube everyday till 14 days. The data was analyzed by Anova test and continued with Duncan test. The result showed that the histopathological features were decrease significantly on P3. The variables of Cox-2 intensity were decrease significantly on P1, P2 and P3. Conclusion of this study was ethanol extract of dragon fruit skin can decrease neoplastic indication of mammary gland on white rats (R. norvegicus) based on histopathological features and Cox-2 inhibitors.

Triptolide inhibits COX-2 expression via NF-kappa B pathway in astrocytes

2006 ◽  
Vol 55 (2) ◽  
pp. 154-160 ◽  
Author(s):  
Yu-Qiao Dai ◽  
Dao-Zhong Jin ◽  
Xing-Zu Zhu ◽  
De-Liang Lei
Keyword(s):  
Nf Kappa B ◽  
Cox 2

scholarly journals The Medicinal Timber Canarium patentinervium Miq. (Burseraceae Kunth.) Is an Anti-Inflammatory Bioresource of Dual Inhibitors of Cyclooxygenase (COX) and 5-Lipoxygenase (5-LOX)

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
R. Mogana ◽  
K. Teng-Jin ◽  
C. Wiart
Keyword(s):  
Inflammatory Diseases ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Frap Assay ◽  
Dual Inhibitor ◽  
Lead Compounds ◽  
Dual Inhibitors ◽  
Cox 2 ◽  
Cox 1

The barks and leaves extracts of Canarium patentinervium Miq. (Burseraceae Kunth.) were investigated for cyclooxygenase (COX) and 5-lipoxygenase (LOX) inhibition via in vitro models. The corresponding antioxidative power of the plant extract was also tested via nonenzyme and enzyme in vitro assays. The ethanolic extract of leaves inhibited the enzymatic activity of 5-LOX, COX-1, and COX-2 with IC50 equal to 49.66±0.02 μg/mL, 0.60±0.01 μg/mL, and 1.07±0.01 μg/mL, respectively, with selective COX-2 activity noted in ethanolic extract of barks with COX-1/COX-2 ratio of 1.22. The ethanol extract of barks confronted oxidation in the ABTS, DPPH, and FRAP assay with EC50 values equal to 0.93±0.01 μg/mL, 2.33±0.02 μg/mL, and 67.00±0.32 μg/mL, respectively, while the ethanol extract of leaves confronted oxidation in β-carotene bleaching assay and superoxide dismutase (SOD) assay with EC50 value of 6.04±0.02 μg/mL and IC50 value of 3.05±0.01 μg/mL. The ethanol extract acts as a dual inhibitor of LOX and COX enzymes with potent antioxidant capacity. The clinical significance of these data is quite clear that they support a role for Canarium patentinervium Miq. (Burseraceae Kunth.) as a source of lead compounds in the management of inflammatory diseases.

scholarly journals COSTUS SPECIOSUS AND ITS ANTIPSORIATIC ARTHRITIS ACTIVITY: A REVIEW

2019 ◽  
Vol 12 (1) ◽  
pp. 30
Author(s):  
Debpratim Chakraborty ◽  
Nisha Lama Yolmo ◽  
Nisha Lama Yolmo
Keyword(s):  
Psoriatic Arthritis ◽  
Side Effect ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
The Other ◽  
Standard Drug ◽  
Traditional Medicines ◽  
Costus Speciosus ◽  
Cox 2 ◽  
Anti Inflammatory

Objective: Psoriatic arthritis is an autoimmune disease. The marketed drugs cannot totally cure the disease state as well as they have severe side effects and for that reason researcher and scientists are going for traditional medicines. Costus speciosus is the plant of choice for its several positive activities against psoriatic arthritis.Methods: Wistar albino healthy rat was taken for 0.1 ml of Freund adjuvant-induced anti-arthritic and anti-inflammatory test, and diclofenac sodium (15 mg/kg) was taken as standard drug. The paw volume was measured at different time 1st, 7th, 14th, and 21st days of experiment. In another study, the protocol is same, but indomethacin (10 mg/kg) was taken as standard drug. Another study was performed to know that the plant drug has any lipopolysaccharide (LPS)-stimulated COX-protein inhibitor activity or not. An acute anti-inflammatory property was studied in carrageenan-induced paw edema and result is measured by plethysmometer. The chronic anti-inflammatory property was studied by cotton pellet-induced granuloma formation. 400 mg/kg and 800 mg/kg dose of ethanolic extract is administered to the animal of both acute and chronic studies.Results: In anti-arthritic and anti-inflammatory study, after the 21st day, it has found that standard drug (diclofenac sodium) decreases paw volume 40 % where the plant drug reduces it 68.33% & 75.50% at higher and lower dose respectively. In the other study the Standard drug (Indomethacin) show arthritic score of 0.83 and the extract shows 1.67 at its higher concentration. In LPS-stimulated cyclooxygenase-2 (COX-2) inhibition study, extract helps to decrease the LPS-stimulated COX-2 protein nearly similar as methotrexate without any side effect. In the cotton pallet-induced anti-inflammatory study, 400 mg/kg and 800 mg/kg dose of ethanolic extract shows similar result against standard drug.Conclusion: Although its activity is less compared to the marketed drug, in the other sides, it has very mild adverse effect compared to the marketed Drug. It can be used as a supportive drug in the treatment of Psoriatic arthritis and by chemical modification the activity may be increased without any side effect.

scholarly journals Structure of the human cyclo-oxygenase-2 gene

1994 ◽  
Vol 302 (3) ◽  
pp. 723-727 ◽  
Author(s):  
S B Appleby ◽  
A Ristimäki ◽  
K Neilson ◽  
K Narko ◽  
T Hla
Keyword(s):  
Genomic Library ◽  
Regulatory Sequences ◽  
Coding Region ◽  
Nf Kappa B ◽  
Protein Coding ◽  
Quiescent Cells ◽  
Cox 2 ◽  
Rate Limiting ◽  
Element Sequence ◽  
Cox 1

Cyclo-oxygenase (Cox), a rate-limiting enzyme in the synthesis of prostanoids, is encoded by two genes, Cox-1 and Cox-2, which are differentially expressed and regulated. Human Cox-1 and -2 polypeptides share 61% primary sequence identity. While the expression of Cox-1 is maximal in quiescent cells. Cox-2 expression is induced by growth factors and cytokines. We have screened a human genomic library with a probe from the 5′-untranslated region (UTR) of the human Cox-2 (hCox-2) cDNA and isolated two overlapping genomic clones. We have determined the DNA sequence of 0.8 kb upstream of the transcription start site, 6 kb of protein coding region, which includes 10 exons and 9 introns, as well as 2.5 kb of the 3′-UTR. The structures of the hCox-1 and hCox-2 and the murine TIS10 (Cox-2) genes are highly conserved, with a few exceptions. The 3′-UTRs of the Cox-1 and -2 genes are distinct; for example, the largest exon in the Cox-2 gene encodes the entire 3′-UTR, containing 22 copies of the ‘AUUUA’ RNA instability element. Sequence analysis of the 5′-flanking region has shown several potential transcription regulatory sequences, including a TATA box, a C/EBP motif, two AP-2 sites, three SP1 sites, two NF-kappa B sites, a CRE motif and an Ets-1 site. These efforts serve as a basis for future studies on transcriptional and post-transcriptional mechanisms of Cox-2 gene regulation.

scholarly journals Anti-Inflammatory Effect of Procyanidins from Wild Grape (Vitis amurensis) Seeds in LPS-Induced RAW 264.7 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Min-Ji Bak ◽  
Van Long Truong ◽  
Hey-Sook Kang ◽  
Mira Jun ◽  
Woo-Sik Jeong
Keyword(s):  
Nitric Oxide ◽  
Mapk Pathway ◽  
Raw 264.7 Cells ◽  
Prostaglandin E ◽  
Vitis Amurensis ◽  
Raw 264.7 ◽  
Wild Grape ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

In the present study, the anti-inflammatory effect and underlying mechanisms of wild grape seeds procyanidins (WGP) were examined using lipopolysaccharide- (LPS-) stimulated RAW 264.7 cells. We used nitric oxide (NO) and prostaglandin E2(PGE2) and reactive oxygen species (ROS) assays to examine inhibitory effect of WGP and further investigated the mechanisms of WGP suppressed LPS-mediated genes and upstream expression by Western blot and confocal microscopy analysis. Our data indicate that WGP significantly reduced NO, PGE2, and ROS production and also inhibited the expression of proinflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions. Consistently, WGP significantly reduced LPS-stimulated expression of proinflammatory cytokines such as tumor necrosis factorα(TNF-α) and interleukin- (IL-) 1β. Moreover, WGP prevented nuclear translocation of nuclear factor-κB (NFκB) p65 subunit by reducing inhibitoryκB-α(IκBα) and NFκB phosphorylation. Furthermore, we found that WGP inhibited LPS-induced phosphorylation of p38 mitogen-activated protein kinase (MAPK). Taken together, our results demonstrated that WGP exerts potent anti-inflammatory activity through the inhibition of iNOS and COX-2 by regulating NFκB and p38 MAPK pathway.

scholarly journals Insulin Suppresses LPS-induced iNOS and COX-2 Expression and NF-κB Activation in Alveolar Macrophages and

2008 ◽  
Vol 22 (1-4) ◽  
pp. 279-286 ◽  
Author(s):  
Joilson Martins ◽  
Matheus Ferracini ◽  
Natalia Ravanelli ◽  
Richardt Landgraf ◽  
Sonia Jancar
Keyword(s):  
Alveolar Macrophages ◽  
Nf Kappa B ◽  
Cox 2
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