scholarly journals Insulin Suppresses LPS-induced iNOS and COX-2 Expression and NF-κB Activation in Alveolar Macrophages and

2008 ◽  
Vol 22 (1-4) ◽  
pp. 279-286 ◽  
Author(s):  
Joilson Martins ◽  
Matheus Ferracini ◽  
Natalia Ravanelli ◽  
Richardt Landgraf ◽  
Sonia Jancar
2006 ◽  
Vol 55 (2) ◽  
pp. 154-160 ◽  
Author(s):  
Yu-Qiao Dai ◽  
Dao-Zhong Jin ◽  
Xing-Zu Zhu ◽  
De-Liang Lei
Keyword(s):  

1994 ◽  
Vol 302 (3) ◽  
pp. 723-727 ◽  
Author(s):  
S B Appleby ◽  
A Ristimäki ◽  
K Neilson ◽  
K Narko ◽  
T Hla

Cyclo-oxygenase (Cox), a rate-limiting enzyme in the synthesis of prostanoids, is encoded by two genes, Cox-1 and Cox-2, which are differentially expressed and regulated. Human Cox-1 and -2 polypeptides share 61% primary sequence identity. While the expression of Cox-1 is maximal in quiescent cells. Cox-2 expression is induced by growth factors and cytokines. We have screened a human genomic library with a probe from the 5′-untranslated region (UTR) of the human Cox-2 (hCox-2) cDNA and isolated two overlapping genomic clones. We have determined the DNA sequence of 0.8 kb upstream of the transcription start site, 6 kb of protein coding region, which includes 10 exons and 9 introns, as well as 2.5 kb of the 3′-UTR. The structures of the hCox-1 and hCox-2 and the murine TIS10 (Cox-2) genes are highly conserved, with a few exceptions. The 3′-UTRs of the Cox-1 and -2 genes are distinct; for example, the largest exon in the Cox-2 gene encodes the entire 3′-UTR, containing 22 copies of the ‘AUUUA’ RNA instability element. Sequence analysis of the 5′-flanking region has shown several potential transcription regulatory sequences, including a TATA box, a C/EBP motif, two AP-2 sites, three SP1 sites, two NF-kappa B sites, a CRE motif and an Ets-1 site. These efforts serve as a basis for future studies on transcriptional and post-transcriptional mechanisms of Cox-2 gene regulation.


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