scholarly journals Characterization of the Antigenic Heterogeneity of Lipoarabinomannan, the Major Surface Glycolipid ofMycobacterium tuberculosis, and Complexity of Antibody Specificities toward This Antigen

2018 ◽  
Vol 200 (9) ◽  
pp. 3053-3066 ◽  
Author(s):  
Alok Choudhary ◽  
Deendayal Patel ◽  
William Honnen ◽  
Zhong Lai ◽  
Raja Sekhar Prattipati ◽  
...  
Keyword(s):  
Ofmycobacterium Tuberculosis ◽  
Antibody Specificities ◽  
Major Surface ◽  
Antigenic Heterogeneity

scholarly journals Structural and Functional Characterization of a Ketosteroid Transcriptional Regulator ofMycobacterium tuberculosis

2014 ◽  
Vol 290 (2) ◽  
pp. 872-882 ◽  
Author(s):  
Adam M. Crowe ◽  
Peter J. Stogios ◽  
Israël Casabon ◽  
Elena Evdokimova ◽  
Alexei Savchenko ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Transcriptional Regulator ◽  
Ofmycobacterium Tuberculosis

Functional Characterization of the Precursor and Spliced Forms of RecA Protein ofMycobacterium tuberculosis†

Biochemistry ◽  
1996 ◽  
Vol 35 (6) ◽  
pp. 1793-1802 ◽  
Author(s):  
R. Ajay Kumar ◽  
Moreshwar B. Vaze ◽  
Nagasuma R. Chandra ◽  
M. Vijayan ◽  
K. Muniyappa
Keyword(s):  
Functional Characterization ◽  
Reca Protein ◽  
Ofmycobacterium Tuberculosis

Characterization of a major surface-associated excretory–secretory antigen ofTrichinella spiralislarvae with antibodies to keyhole limpet haemocyanin

Parasitology ◽  
1994 ◽  
Vol 109 (4) ◽  
pp. 531-538 ◽  
Author(s):  
J. Modha ◽  
W. M. Robertson ◽  
M. W. Kennedy ◽  
J. R. Kusel
Keyword(s):  
Trichinella Spiralis ◽  
Cross Reactivity ◽  
Ammonium Bromide ◽  
Surface Coat ◽  
Keyhole Limpet Haemocyanin ◽  
Secretory Antigen ◽  
Cetyltrimethyl Ammonium ◽  
Major Surface ◽  
And Function

SUMMARYA multi-subunit antigen (nativeMr> 200 kDa, reducedMr97–100 kDa) has been identified in homogenates ofTrichinella spiralislarvae using affinity-purified rabbit anti-keyhole limpet haemocyanin (KLH) antibodies and its cross-reactivity with KLH was confirmed by competition blotting. The antigen was not present at the larval surface but was exposed after treatment of the larvae with the detergent cetyltrimethyl ammonium bromide (CTAB) which removed the surface coat. This correlated with a significant decrease in insertion of the surface-restricted fluorescent lipid probe AF18, indicating that the surface coat must be lipidic in nature. Unlike KLH, the larval antigen blotted onto nitrocellulose was itself periodate insensitive. Periodate treatment of whole larvae, however, resulted in shedding of the surface, to which anti-KLH antibodies then bound intensely. Anti-KLH antibodies also recognized three (49, 55, 108 kDa) of the four most dominant antigens in excretory–secretory (ES) products of cultured larvae, whose excretion–secretion was increased with CTAB. The nature, location and function of the antigen is discussed.

Characterization of the phosphorylation sites ofMycobacterium tuberculosis serine/threonine protein kinases, PknA, PknD, PknE, and PknH by mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (13) ◽  
pp. 3754-3766 ◽  
Author(s):  
Virginie Molle ◽  
Isabelle Zanella-Cleon ◽  
Jean-Philippe Robin ◽  
Souen Mallejac ◽  
Alain J. Cozzone ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Kinases ◽  
Phosphorylation Sites ◽  
Ofmycobacterium Tuberculosis

scholarly journals Characterization of Anaplasma marginale Isolated from North American Bison

2003 ◽  
Vol 69 (8) ◽  
pp. 5001-5005 ◽  
Author(s):  
José de la Fuente ◽  
Elizabeth J. Golsteyn Thomas ◽  
Ronald A. Van Den Bussche ◽  
Robert G. Hamilton ◽  
Elaine E. Tanaka ◽  
...  
Keyword(s):  
New World ◽  
Surface Protein ◽  
Anaplasma Marginale ◽  
The United States ◽  
Bison Bison ◽  
Infected Cattle ◽  
Major Surface Protein ◽  
The World ◽  
Major Surface

ABSTRACT Anaplasma marginale (Rickettsiales: Anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world. Although serologic tests have identified American bison, Bison bison, as being infected with A. marginale, the present study was undertaken to confirm A. marginale infection and to characterize isolates obtained from naturally infected bison in the United States and Canada. Major surface protein (MSP1a and MSP4) sequences of bison isolates were characterized in comparison with New World cattle isolates. Blood from one U.S. bison was inoculated into a susceptible, splenectomized calf, which developed acute anaplasmosis, demonstrating infectivity of this A. marginale bison isolate for cattle. The results of this study showed that these A. marginale isolates obtained from bison were similar to ones from naturally infected cattle.

scholarly journals Characterization of Major Surface Glycoprotein Genes of Human Pneumocystis carinii and High-Level Expression of a Conserved Region

1998 ◽  
Vol 66 (9) ◽  
pp. 4268-4273 ◽  
Author(s):  
Qin Mei ◽  
Ross E. Turner ◽  
Vivian Sorial ◽  
Diane Klivington ◽  
C. William Angus ◽  
...  
Keyword(s):  
Genomic Library ◽  
Pneumocystis Carinii ◽  
Surface Glycoprotein ◽  
High Level Expression ◽  
Serum Samples ◽  
Major Surface Glycoprotein ◽  
Adult Humans ◽  
Major Surface ◽  
High Level

ABSTRACT To facilitate studies of Pneumocystis cariniiinfection in humans, we undertook to better characterize and to express the major surface glycoprotein (MSG) of human P. carinii, an important protein in host-pathogen interactions. Seven MSG genes were cloned from a single isolate by PCR or genomic library screening and were sequenced. The predicted proteins, like rat MSGs, were closely related but unique variants, with a high level of conservation among cysteine residues. A conserved immunodominant region (of approximately 100 amino acids) near the carboxy terminus was expressed at high levels in Escherichia coli and used in Western blot studies. All 49 of the serum samples, which were taken from healthy controls as well as from patients with and withoutP. carinii pneumonia, were reactive with this peptide by Western blotting, supporting the hypothesis that most adult humans have been infected with P. carinii at some point. This recombinant MSG fragment, which is the first human P. carinii antigen available in large quantities, may be a useful reagent for investigating the epidemiology of P. cariniiinfection in humans.

scholarly journals Development of a Multivalent ISCOM Vaccine against Anaplasmosis

1993 ◽  
Author(s):  
Guy H. Palmer ◽  
Eugene Pipano ◽  
Terry F. McElwain ◽  
Varda Shkap ◽  
Donald P. Knowles, Jr.
Keyword(s):  
T Lymphocytes ◽  
Outer Membrane ◽  
Membrane Surface ◽  
Surface Protein ◽  
The United States ◽  
Surface Proteins ◽  
Efficient Production ◽  
Major Surface ◽  
Homologous Challenge

Anaplasmosis is an arthropod+borne disease of cattle caused by the rickettsia Anaplasma marginale and an impediment to efficient production of healthy livestock in both Israel and the United States. Our research focuses on development of a recombinant membrane surface protein (MSP) immunogen to replace current vaccines derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Briefly, we accomplished the following in our BARD supported research: i) characterization of the intramolecular and intermolecular relationships of the native Major Surface Proteins (MSP) in the outer membrane; ii) expression, purification, and epitope characterization of the recombinant MSP-2, MSP-3, MSP-4, and MSP-5 proteins required to construct the recombinant ISCOM; iii) demonstration that the outer membrane-Quil A induces CD4+ T lymphocytes specific for the outer membrane polypeptides; iv) identification of CD4+ T lymphocytes that recognize outer membrane polypeptide epitopes conserved among other wise antigenically distinct strains; v) determination that immunization with the outer membrane-Quil A construct does not affect the ability of ticks to acquire or transmit A. marginale; and vi) demonstration that the outer membrane-Quil A construct induces complete protection against rickettsemia upon homologous challenge and significant protection against challenge with antigenically distinct strains, including tick transmission. Importantly, the level of protection against homologous challenge in the MSP vaccinates was comparable to that induced by live blood-based vaccines and demonstrates that development of a new generation of vaccines is feasible.

Characterization of Major Surface Glycoprotein Genes of Human Pneumocystis carinii and High-Level Expression of a Conserved Region

1998 ◽  
Vol 66 (9) ◽  
pp. 4268-4273
Author(s):  
Qin Mei ◽  
Ross E. Turner ◽  
Vivian Sorial ◽  
Diane Klivington ◽  
C. William Angus ◽  
...  
Keyword(s):  
Pneumocystis Carinii ◽  
Surface Glycoprotein ◽  
High Level Expression ◽  
Major Surface Glycoprotein ◽  
Conserved Region ◽  
Major Surface ◽  
High Level
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