Characterization of the phosphorylation sites ofMycobacterium tuberculosis serine/threonine protein kinases, PknA, PknD, PknE, and PknH by mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (13) ◽  
pp. 3754-3766 ◽  
Author(s):  
Virginie Molle ◽  
Isabelle Zanella-Cleon ◽  
Jean-Philippe Robin ◽  
Souen Mallejac ◽  
Alain J. Cozzone ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Kinases ◽  
Phosphorylation Sites ◽  
Ofmycobacterium Tuberculosis

scholarly journals Characterization of Phosphorylated Proteins Using Mass Spectrometry

2020 ◽  
Vol 21 ◽  
Author(s):  
Li-Rong Yu ◽  
Timothy D. Veenstra
Keyword(s):  
Gene Expression ◽  
Mass Spectrometry ◽  
Phosphorylation Sites ◽  
Post Translational Modification ◽  
Specific Antibodies ◽  
Phosphorylated Proteins

Abstract:: Phosphorylation is arguably the most important post-translational modification that occurs within proteins. Phosphorylation is used as a signal to control numerous physiological activities ranging from gene expression to metabo-lism. Identifying phosphorylation sites within proteins was historically a challenge as it required either radioisotope label-ing or the use of phospho-specific antibodies. The advent of mass spectrometry (MS) has had a major impact on the abil-ity to qualitatively and quantitatively characterize phosphorylated proteins. In this article we describe MS methods for characterizing phosphorylation sites within individual proteins as well as entire proteome samples. The utility of these methods is illustrated in examples that show the information that can be gained using these MS techniques.

Systematic characterization of phosphorylation sites in NFATc2 by linear ion trap mass spectrometry

PROTEOMICS ◽  
2006 ◽  
Vol 6 (S1) ◽  
pp. S16-S27 ◽  
Author(s):  
Margarita Villar ◽  
Inmaculada Ortega-Pérez ◽  
Felipe Were ◽  
Eva Cano ◽  
Juan Miguel Redondo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry ◽  
Linear Ion Trap ◽  
Phosphorylation Sites

Characterization of Phosphorylation Sites on Histone H1 Isoforms by Tandem Mass Spectrometry

2004 ◽  
Vol 3 (6) ◽  
pp. 1219-1227 ◽  
Author(s):  
Benjamin A. Garcia ◽  
Scott A. Busby ◽  
Cynthia M. Barber ◽  
Jeffrey Shabanowitz ◽  
C. David Allis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Histone H1 ◽  
Tandem Mass ◽  
Phosphorylation Sites
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