The Influence of a Virus Disease and Parasites on Spilonota ocellana in Apple Orchards

1966 ◽  
Vol 98 (10) ◽  
pp. 1035-1045 ◽  
Author(s):  
R. P. Jaques ◽  
H. T. Stultz
Keyword(s):  
Nova Scotia ◽  
Nuclear Polyhedrosis Virus ◽  
Virus Disease ◽  
Host Population ◽  
Apple Orchards ◽  
Late Instar ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Favorable Conditions

AbstractA nuclear-polyhedrosis virus disease of Spilonota ocellana (Denis and Schiffermüller) is described. The disease was found in the majority of the Nova Scotia apple orchards sampled in a nine-year survey. Under favorable conditions the disease caused high mortalities but usually less than 10 per cent of a host population was killed by the virus.Agathis laticinctus (Cresson) killed an average of 25 per cent of late-instar larvae.

A VIRUS DISEASE OF ECTROPIS CREPUSCULARIA SCHIFF. (GEOMETRIDAE: LEPIDOPTERA)

1967 ◽  
Vol 13 (7) ◽  
pp. 855-858 ◽  
Author(s):  
Oswald N. Morris
Keyword(s):  
Dna Synthesis ◽  
Deoxyribonucleic Acid ◽  
Nuclear Polyhedrosis Virus ◽  
Virus Disease ◽  
Disease Process ◽  
Virus Diseases ◽  
Virus Particles ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The morphology of a nuclear polyhedrosis virus of Ectropis crepuscularia Schiff., the histopathology of the disease, and deoxyribonucleic acid (DNA) synthesis during the disease process were studied. The polyhedral inclusions measure 1.4 μ in diameter with a range of 0.8–2.1 μ. The virus particles are rods measuring 319 mμ by 79 mμ with a range of 280–360 mμ by 70–110 mμ. DNA labelling and nuclear swelling follow the course of previously described polyhedrosis virus diseases.

Transmission of Virus in Tent Caterpillar Populations

1966 ◽  
Vol 98 (10) ◽  
pp. 1100-1104 ◽  
Author(s):  
G. R. Stairs
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Virus Disease ◽  
Malacosoma Disstria ◽  
Forest Tent Caterpillar ◽  
Larval Instars ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Tent Caterpillar

AbstractThe transmission of nuclear polyhedrosis virus in populations of the forest tent caterpillar, Malacosoma disstria (Hübner), was studied in Sudbury district, Ontario. Virus was transmitted from generation to generation by infected adults. Their progeny died from virus disease during the second and third larval instars. Adult flies of Sarcophaga aldrichi Parker, a dipterous parasite, were attracted to these dead, diseased larvae, became contaminated with virus, and spread the virus to foliage on which healthy larvae were feeding. The importance of these disseminating agents in the development of virus epizootics is discussed.

scholarly journals Bombyx mori Pupae Efficiently Produce Recombinant AAV2/HBoV1 Vectors with a Bombyx mori Nuclear Polyhedrosis Virus Expression System

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 704
Author(s):  
Qian Yu ◽  
Pengfei Chang ◽  
Xiaoxuan Liu ◽  
Peng Lü ◽  
Qi Tang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bombyx Mori ◽  
Nuclear Polyhedrosis Virus ◽  
Expression System ◽  
Efficient Production ◽  
Human Bocavirus ◽  
Adeno Associated Virus ◽  
Silkworm Pupae ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Recombinant adeno-associated virus (AAV) vectors have broad application prospects in the field of gene therapy. The establishment of low-cost and large-scale manufacturing is now the general agenda for industry. The baculovirus-insect cell/larva expression system has great potential for these applications due to its scalability and predictable biosafety. To establish a more efficient production system, Bombyx mori pupae were used as a new platform and infected with recombinant Bombyx mori nuclear polyhedrosis virus (BmNPV). The production of a chimeric recombinant adeno-associated virus (rAAV) serotype 2/human bocavirus type-1 (HBoV1) vector was used to evaluate the efficiency of this new baculovirus expression vector (BEV)–insect expression system. For this purpose, we constructed two recombinant BmNPVs, which were named rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP. The yields of rAAV2/HBoV1 derived from the rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP co-infected BmN cells exceeded 2 × 104 vector genomes (VG) per cell. The rBmNPV/AAV2Rep-HBoV1Cap and rBmNPV/AAV2ITR-eGFP can express stably for at least five passages. Significantly, rAAV2/HBoV1 could be efficiently generated from BmNPV-infected silkworm larvae and pupae at average yields of 2.52 × 1012 VG/larva and 4.6 × 1012 VG/pupa, respectively. However, the vectors produced from the larvae and pupae had a high percentage of empty particles, which suggests that further optimization is required for this platform in the future. Our work shows that silkworm pupae, as an efficient bioreactor, have great potential for application in the production of gene therapy vectors.

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