EFFECTS OF NUCLEAR POLYHEDROSIS VIRUS AND BACILLUS THURINGIENSIS ON WESTERN SPRUCE BUDWORM (LEPIDOPTERA: TORTRICIDAE) 1 AND 2 YEARS AFTER AERIAL APPLICATION

1982 ◽  
Vol 114 (3) ◽  
pp. 281-282 ◽  
Author(s):  
R. F. Shepherd ◽  
T. G. Gray ◽  
J. C. Cunningham
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Spruce Budworm ◽  
Western Spruce Budworm ◽  
Aerial Application ◽  
Insect Populations ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
The Impact ◽  
Selection Of

An understanding of all environmental effects resulting from control actions is important in the selection of a method of managing insect populations. Effects may extend beyond the year of treatment and can modify management decisions. In 1978, a nuclear polyhedrosis virus (NPV) and Bacillus thuringiensis Berliner (B.t.) were tested as aerial spray applications against the western spruce budworm, Choristoneura occidentalis Freeman, on Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, near Cache Creek, British Columbia. The impact on population densities and on the incidence of NPV was measured in the treated and non-treated areas for 2 vears following the year of application, and is reported here.

AERIAL APPLICATION OF TWO BACULOVIRUSES AGAINST THE WESTERN SPRUCE BUDWORM, CHORISTONEURA OCCIDENTALIS FREEMAN (LEPIDOPTERA: TORTRICIDAE), IN BRITISH COLUMBIA

1989 ◽  
Vol 121 (3) ◽  
pp. 209-217 ◽  
Author(s):  
I.S. Otvos ◽  
J.C. Cunningham ◽  
W.J. Kaupp
Keyword(s):  
British Columbia ◽  
Nuclear Polyhedrosis Virus ◽  
Adult Emergence ◽  
Spruce Budworm ◽  
Choristoneura Occidentalis ◽  
Western Spruce Budworm ◽  
Granulosis Virus ◽  
Population Reduction ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

AbstractTwo viruses, one a nuclear polyhedrosis virus and the other a granulosis virus, were applied in an attempt to initiate epizootics in populations of western spruce budworm, Choristoneura occidentalis Freeman, on Douglas-fir trees, Pseudotsuga menziesii (Mirb.) Franco, in southeastern British Columbia. Two 172-ha plots were aerially treated in 1982 with 9.0 kg of lyophilized, virus-infected larval powder that was formulated in an emulsifiable oil tank mix and applied at 9.4 L per hectare. Each plot was treated when larval populations were at the peak of the fourth instar. The nuclear polyhedrosis virus was applied at 5.4 × 1011 polyhedral inclusion bodies per hectare and the granulosis virus at 1.7 × 1014 capsules per hectare. Results showed that the granulosis virus treatment caused 34.6% population reduction (Abbott’s formula) and the nuclear polyhedrosis virus 51.8%. Larvae from treated and check plots were reared individually in the laboratory and the incidence of viruses, parasitoids, and successful adult emergence was recorded. Studies m these plots continued in 1983 and 1984. Although vertical transmission of both viruses was evident, their impact on budworm mortality was less than in 1982. Consequently, the epizootics were not sufficiently intense to control the target insect population.

AERIAL APPLICATION OF NUCLEAR POLYHEDROSIS VIRUS AGAINST DOUGLAS-FIR TUSSOCK MOTH, ORGYIA PSEUDOTSUGATA (McDUNNOUGH) (LEPIDOPTERA: LYMANTRIIDAE): II. IMPACT 1 AND 2 YEARS AFTER APPLICATION

1987 ◽  
Vol 119 (7-8) ◽  
pp. 707-715 ◽  
Author(s):  
I.S. Otvos ◽  
J.C. Cunningham ◽  
R.I. Alfaro
Keyword(s):  
Tree Mortality ◽  
Nuclear Polyhedrosis Virus ◽  
Douglas Fir ◽  
Aerial Application ◽  
Moth Population ◽  
Tussock Moth ◽  
Orgyia Pseudotsugata ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
The Impact

AbstractFollowing aerial application of a Douglas-fir tussock moth, Orgyia pseudotsugata (McDunnough), nuclear polyhedrosis virus (NPV) product called Virtuss on four plots in 1982, observations were made to determine the impact of these treatments in 1983 and 1984. Treated plots as well as buffer zones between and adjoining the treated plots, and three of the four check plots established in 1982, were monitored. The NPV appeared to have spread from the treated plots to adjoining areas in 1982, effectively reducing the Douglas-fir tussock moth population. This observation suggests that a strategy of spraying alternate swaths of Douglas-fir tussock moth infested stands with this viral insecticide may effectively initiate an epizootic that would control the population at a reduced cost.A naturally occurring NPV epizootic decimated the Douglas-fir tussock moth population in the three check plots in 1983, but severe tree mortality occurred in two of these plots with 60 and 62% of sample trees dead in 1984. Light tree mortality was noted in 1984 in two of the four treated plots with 4 and 7% of sample trees killed. It is concluded that the virus treatments in 1982 were successful in preventing tree mortality.

scholarly journals Laboratorium Sederhana Perbanyakan Agensia Hayati Pengendali Hama dan Penyakit Tanaman Padi di PP Gapsera Sejahtera Madiri Lampung Tengah

2020 ◽  
Vol 1 (1) ◽  
pp. 73-79
Author(s):  
- Ivayani
Keyword(s):  
Bacillus Thuringiensis ◽  
Nuclear Polyhedrosis Virus ◽  
Air Flow ◽  
Polyhedrosis Virus ◽  
Laminar Air Flow ◽  
Nuclear Polyhedrosis

Kecamatan Seputih Raman Kabupaten Lampung Tengah merupakan salah satu daerah penghasil komoditi padi yang memasok persediaan beras di Provinsi Lampung. Gapoktan Gapsera sejahtera Mandiri merupakan salah satu Gapoktan di Kecamatan Seputih Raman yang sudah menerapkan pertanian organik dalam budidaya tanamana padi. Salah satu produk yang dihasilkan oleh Gapoktan Subur Asri adalah beras organik “Berasera”. Dalam proses budidaya dan pengendalian hama peyakit Gapoktan Subur Asri sudah tidak menggunakan bahan kimia sintetik melainkan dengan menggunakan agensia hayati. Akan tetapi dalam perbanyakan agensia hayati (mikroorganisme) belum maksimal karena kurangnya pengetahuan mengenai teknik perbanyakan agensia hayati, selain itu juga tidak tersedianya tempat kerja perbanyakan yang tidak aseptik (steril) sehingga terjadinya kontaminasi dengan mikroorganisme lainnya. Oleh karena itu untuk menciptakan lingkungan perbanyakan agensia hayati yang aseptik maka diperlukan suatu Laboratorium sederhana yang terdiri dari alat-alat standar Laboratorium. Metode yang digunakan adalah pembimbingan melalui sosialisasi teknik perbanyakan agensia hayati, pendampingan dalam mencari agensia hayati di lapangan dan pendampingan pembuatan laboratorium sederhana. Kegiatan yang telah dilaksanakan di PP Gapsera Sejahtera Mandiri adalah Penyuluhan mengenai cara isolasi agensia hayati NPV (Nuclear Polyhedrosis Virus) dan isolasi Bt (Bacillus thuringiensis) serta teknik perbanyakannya, Teknik bekerja secara aseptik dan Pembuatan Laboratorium sederhana yang salah satu alatnya ialah Enkas (Lemari kerja aseptik) / Laminar air flow. Berdasarkan kegiatan yang telah dilakukan, petani mampu melakukan isolasi NPV dan Bt, serta mampu bekerja secara aseptik guna mendapatkan/ memperbanyak agensia hayati pengendali hama dan penyakit tumbuhan. Luaran dari kegiatan ini adalah tersedianya laboratorium sederhana yang operasioanal untuk perbanyakan agensia hayati pengendali hama dan penyakit tanaman.

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