REACTIONS OF SMALL MAMMALS TO VITAL DYES

1968 ◽  
Vol 100 (5) ◽  
pp. 476-477 ◽  
Author(s):  
Charles H. Buckner
Keyword(s):  
Small Mammals ◽  
Rhodamine B ◽  
Field Experiments ◽  
Nile Blue ◽  
Vital Dyes ◽  
Larch Sawfly

AbstractStudies were conducted on the reactions of small mammals to the vital dyes Rhodamine B and Nile Blue. The dyes are currently in use as biological tracers for the larch sawfly. Two species of voles and two of four individual shrews reacted indiscriminately to dyed and undyed material: the reactions of the two remaining shrews varied. It was concluded that the behavior of small mammals would not affect seriously the results of field experiments using insects labeled with these dyes.

VITAL DYES AS MARKERS FOR BEHAVIORAL AND POPULATION STUDIES OF THE LARCH SAWFLY, PRISTIPHORA ERICHSONII (HYMENOPTERA: TENTHREDINIDAE)

1968 ◽  
Vol 100 (5) ◽  
pp. 470-475 ◽  
Author(s):  
R. J. Heron
Keyword(s):  
Rhodamine B ◽  
Population Studies ◽  
Nile Blue ◽  
Larix Laricina ◽  
Vital Dyes ◽  
Flight Range ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Adult Dispersal ◽  
Dye Retention

AbstractLarvae of the larch sawfly, Pristiphora erichsonii (Htg.), were fed foliage of tamarack, Larix laricina (Du Roi) K. Koch, treated with solutions of the vital dyes, Nile blue sulphate, rhodamine B, and coriphosphine O, to determine their suitability as markers. Coriphosphine O was highly toxic at the concentrations administered but successful tagging was obtained with the other two dyes. Optimal dye retention and survival were obtained when larvae were fed for 3 days early in the last stadium using Nile blue sulphate and throughout the last stadium using rhodamine B.Both dyes persisted throughout development and were incorporated in the cocoons and the oviposited eggs. They could be readily seen in all stages except for the cocoons and for eggs that contained rhodamine B. The dyes could be detected in cocoons by treating them with cold KOH followed by ethanol. The presence of rhodamine B in the eggs could be ascertained by its yellow fluorescence in ultraviolet.The dyes incorporated in the cocoons are relatively stable and laboratory tests indicate they probably can withstand weathering under normal field conditions over a 1-year period.The use of these dyes for internal marking should prove useful in studying adult dispersal and flight range and as a technique for use in population studies of the larch sawfly.

Bait uptake by free living brush-tailed phascogales Phascogale tapoatafa and other non-target mammals during simulated buried fox baiting.

2003 ◽  
Vol 25 (1) ◽  
pp. 31 ◽  
Author(s):  
D Fairbridge ◽  
R Anderson ◽  
T Wilkes ◽  
G Pell
Keyword(s):  
Small Mammals ◽  
Rhodamine B ◽  
Field Trials ◽  
Future Research ◽  
Research Needs ◽  
Trichosurus Vulpecula ◽  
Free Living ◽  
Central Victoria ◽  
Petaurus Breviceps

Field trials were undertaken in box-ironbark woodland at Puckapunyal Military Area (PMA) in central Victoria between January 2000 and April 2001 to assess bait uptake by the brushtailed phascogale (Phascogale tapoatafa) and other small mammals during simulated fox baiting exercises. The systemic marker Rhodamine B was used in non-toxic fox baits (Foxoff®) to detect non-target bait consumption. The trials demonstrated that free-living brush-tailed phascogales, yellow-footed antechinus (Antechinus flavipes), sugar gliders (Petaurus breviceps) and common brushtail possums (Trichosurus vulpecula) were capable of accessing non-toxic fox baits buried under 10 cm of sand. Rhodamine B markings were detected in six (15%) of 40 P. tapoatafa captured during the study period. The implications of these results and future research needs are discussed.

Mortality of Cocoons of the Larch Sawfly, Pristiphora erichsonii (Htg.), in Relation to Distance from Small-Mammal Tunnels

1959 ◽  
Vol 91 (9) ◽  
pp. 535-542 ◽  
Author(s):  
C. H. Buckner
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Natural Mortality ◽  
Mortality Factors ◽  
Forest Reserve ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
The Relationship

The relationship between the fate of cocoons of the larch sawfly, Pristiphora erichsonii (Htg.), and distance from small-mammal tunnels was studied during 1958 in the Whiteshell Forest Reserve of eastern Manitoba. The objects were to determine the distance that small mammals can detect cocoons and to observe possible effects of the interactions of small-mammal predation and other natural mortality factors of the insect. Additional analyses of the data provided information on the behaviour of the predators and the ecology of the prey insect.

A simple technique for the preservation of vital dyes in fixed and sectioned embryos

Development ◽  
1966 ◽  
Vol 15 (2) ◽  
pp. 131-132
Author(s):  
J. Pertusa
Keyword(s):  
Methylene Blue ◽  
Ethyl Alcohol ◽  
Toluidine Blue ◽  
Trypan Blue ◽  
Simple Technique ◽  
Nile Blue ◽  
Neutral Red ◽  
The Other ◽  
Serial Sectioning ◽  
Vital Dyes

In embryological work using vital dyes it is highly desirable to be able to study the distribution of the dyes in fixed material, whether examined in toto or after embedding in paraffin and serial sectioning. However, both fixation and dehydration present problems for the preservation of colour in vitally stained cells. Some fixatives preserve some dyes but, so far as I am aware, none will preserve all the vital dyes in common use. On the other hand, ethyl alcohol destroys or dissolves all vital dyes and its use in dehydration is thus undesirable. Among the fixatives that have been proposed are those of Golowin (1902), Mitamura (1923), Parat & Painlevé (1925), and Tchéou Tai Chuin (1930) for neutral red; that of Izquierdo (1955) for toluidine blue; that of Gérard (1925) for Trypan blue; that of Turchini (1919) for methylene blue; that of Lehmann (1929) for Nile blue.

A Strain of Bacillus cereus Fr. and Fr. Pathogenic for the Larch Sawfly, Pristiphora erichsonii (Htg.)

1954 ◽  
Vol 86 (2) ◽  
pp. 73-77 ◽  
Author(s):  
A. M. Heimpel
Keyword(s):  
Bacillus Cereus ◽  
Field Experiments ◽  
Positive Correlation ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
The Mean

AbstractA strain of Bacillus cereus (Pr-1017) was isolated from dead and dying larvae of the larch sawfly. Laboratory and field feeding tests resulted in mortalities as high as 60 per cent and 38 per cent, respectively.A positive correlation between the incidence of mortality and the mean daily temperatures was obtained in the field experiments.

Using Rhodamine B to Assess the Movement of Small Mammals in an Urban Slum

2021 ◽  
Author(s):  
Adedayo Michael Awoniyi ◽  
Fábio Neves Souza ◽  
Caio Graco Zeppelini ◽  
Bárbara Inês A. Xavier ◽  
Ana Maria Barreto ◽  
...  
Keyword(s):  
Small Mammals ◽  
Rhodamine B ◽  
Urban Slum

The Assessment of Larch Sawfly Cocoon Predation by Small Mammals

1959 ◽  
Vol 91 (5) ◽  
pp. 275-282 ◽  
Author(s):  
C. H. Buckner
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Life Tables ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Forest Biology ◽  
Biology Laboratory ◽  
Adequate Method

Current investigations at the Forest Biology Laboratory, Winnipeg, are being directed towards the formulation of life tables for the larch sawfly, Pristiphora erichsonii (Htg.) (Lejeune, 1955). Because small mammal predation is a major factor in reducing cocoon populations of the sawfly (Buckner, 1953), an adequate method of assessing this source of mortality is desirable

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