VITAL DYES AS MARKERS FOR BEHAVIORAL AND POPULATION STUDIES OF THE LARCH SAWFLY, PRISTIPHORA ERICHSONII (HYMENOPTERA: TENTHREDINIDAE)

1968 ◽  
Vol 100 (5) ◽  
pp. 470-475 ◽  
Author(s):  
R. J. Heron
Keyword(s):  
Rhodamine B ◽  
Population Studies ◽  
Nile Blue ◽  
Larix Laricina ◽  
Vital Dyes ◽  
Flight Range ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Adult Dispersal ◽  
Dye Retention

AbstractLarvae of the larch sawfly, Pristiphora erichsonii (Htg.), were fed foliage of tamarack, Larix laricina (Du Roi) K. Koch, treated with solutions of the vital dyes, Nile blue sulphate, rhodamine B, and coriphosphine O, to determine their suitability as markers. Coriphosphine O was highly toxic at the concentrations administered but successful tagging was obtained with the other two dyes. Optimal dye retention and survival were obtained when larvae were fed for 3 days early in the last stadium using Nile blue sulphate and throughout the last stadium using rhodamine B.Both dyes persisted throughout development and were incorporated in the cocoons and the oviposited eggs. They could be readily seen in all stages except for the cocoons and for eggs that contained rhodamine B. The dyes could be detected in cocoons by treating them with cold KOH followed by ethanol. The presence of rhodamine B in the eggs could be ascertained by its yellow fluorescence in ultraviolet.The dyes incorporated in the cocoons are relatively stable and laboratory tests indicate they probably can withstand weathering under normal field conditions over a 1-year period.The use of these dyes for internal marking should prove useful in studying adult dispersal and flight range and as a technique for use in population studies of the larch sawfly.

REACTIONS OF SMALL MAMMALS TO VITAL DYES

1968 ◽  
Vol 100 (5) ◽  
pp. 476-477 ◽  
Author(s):  
Charles H. Buckner
Keyword(s):  
Small Mammals ◽  
Rhodamine B ◽  
Field Experiments ◽  
Nile Blue ◽  
Vital Dyes ◽  
Larch Sawfly

AbstractStudies were conducted on the reactions of small mammals to the vital dyes Rhodamine B and Nile Blue. The dyes are currently in use as biological tracers for the larch sawfly. Two species of voles and two of four individual shrews reacted indiscriminately to dyed and undyed material: the reactions of the two remaining shrews varied. It was concluded that the behavior of small mammals would not affect seriously the results of field experiments using insects labeled with these dyes.

HEAD CAPSULE MEASUREMENTS OF THE LARCH SAWFLY, PRISTIPHORA ERICHSONII (HYMENOPTERA: TENTHREDINIDAE) IN KOUCHIBOUGUAC NATIONAL PARK, NEW BRUNSWICK

1980 ◽  
Vol 112 (1) ◽  
pp. 111-112 ◽  
Author(s):  
Patrick Maltais
Keyword(s):  
New Brunswick ◽  
Prince Edward Island ◽  
National Park ◽  
Developmental Stages ◽  
Head Capsule ◽  
Larix Laricina ◽  
Maritime Provinces ◽  
Eastern Canada ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly

The larch sawfly, Pristiphora erichsonii (Htg.), is a serious defoliator of trees of the genus Larix and particularly of Larix laricina (Du Roi) K. Koch in eastern Canada. According to Reeks (1954), four outbreaks occurred in the Maritime Provinces between 1883 and 1942. Following the last outbreak, few specimens of the insect were found in New Brunswick, Nova Scotia, and Prince Edward Island until 1960 when an outbreak started near Fredericton, New Brunswick. Since, outbreaks varying in degree from moderate to severe have persisted throughout this province.In the course of a study on the ecology and population dynamics of the insect it was found necessary to verify and separate the various developmental stages of the larvae.

Population and Mortality Assessment During the Egg and Larval Stages of the Larch Sawfly, Pristiphora erichsonii (Htg.)

1962 ◽  
Vol 94 (3) ◽  
pp. 256-268 ◽  
Author(s):  
W. G. H. Ives
Keyword(s):  
Population Studies ◽  
Alternative Methods ◽  
Larval Feeding ◽  
Sampling Techniques ◽  
Larval Stages ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Foliage Weight

This contribution is the eighth in a series of papers on sampling techniques used in population studies of the larch sawfly (Ives, 1955; Ives and Prentice, 1958; Buckner, 1959; Ives, 1959; Ives and Prentice, 1959; Ives and Turnock, 1959; and Turnock, 1960). Although the greatest proportionate mortality usually occurs in the cocoon stage of this insect, the largest amount of mortality in terms of absolute numbers occurs between oviposition and the completion of larval feeding. This paper presents a method for estimating seasonal increments to the egg and larval populations and alternative methods for estimating mortality during these stages. Examples are also provided of the integration of these methods with previously developed techniques for estimating egg populations and foliage weight.

A Sequential Sampling Technique for Surveys of the Larch Sawfly

1958 ◽  
Vol 90 (6) ◽  
pp. 331-338 ◽  
Author(s):  
W. G. H. Ives ◽  
R. M. Prentice
Keyword(s):  
Sampling Methods ◽  
Population Studies ◽  
Sequential Sampling ◽  
Sampling Technique ◽  
Large Sample Size ◽  
Forest Reserve ◽  
Forest Insects ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly

Sequential sampling methods have been developed for population surveys of a number of forest insects (4, 6, 7, 9). These methods permit rapid classification of populations into broad infestation classes with predetermined accuracy and a minimum of sampling, and are useful for mapping and recording widespread infestations.The method of assessing egg populations of the larch sawfly, Pristiphora erichsonii (Htg.), developed by Ives (3) for intensive population studies, was not suitable for surveys because of the large sample size required. A sequential plan for the larch sawfly using hypothetical infestation classes was briefly reviewed by Ives (2). This plan is modified in the present paper in accordance with new data that have accumulated from observations on plots in the Whiteshell Forest Reserve and other stands in Manitoba and Saskatchewan.

RECENT OUTBREAK OF THE LARCH SAWFLY, PRISTIPHORA ERICHSONII (HARTIG), IN SUBARCTIC QUÉBEC

1991 ◽  
Vol 123 (3) ◽  
pp. 611-619 ◽  
Author(s):  
Conrad Cloutier ◽  
Louise Filion
Keyword(s):  
General Characteristic ◽  
Climatic Conditions ◽  
Population Increase ◽  
Larix Laricina ◽  
Sudden Increase ◽  
Frequency Distributions ◽  
Pristiphora Erichsonii ◽  
Shoot Production ◽  
Larch Sawfly ◽  
Long Shoots

AbstractLarch sawfly oviposition activity as revealed by scarring of long shoots of eastern larch, Larix laricina (DuRoi) K. Koch, was measured at various locations in the high boreal forest and forest tundra in Québec in 1988 and 1989. The data show that larch sawfly is established up to the tree line, even on isolated larch growing under climatic conditions that are extreme for this tree. Frequency distributions of scarred shoots as a function of time suggest thai larch sawfly populations reached outbreak levels in the 1980s, with peak numbers in 1981 for the high boreal, and in 1985 for the subarctic regions sampled. Trends in long shoot production by larch trees started to fluctuate simultaneously with the sudden increase in larch sawfly populations along the Grande Rivière de la Baleine in 1984. In this region in 1985, the proportion of long shoots used by Pristiphora erichsonii averaged 20–35%, which may have limited further population increase. Although foliage reduction and branch mortality were observed, mortality of whole trees was not a general characteristic of this outbreak.

HOST DERIVED CHEMICALS: THE BASIS FOR PREFERENTIAL FEEDING BEHAVIOR OF THE LARCH SAWFLY, PRISTIPHORA ERICHSONII (HYMENOPTERA: TENTHREDINIDAE), ON TAMARACK, LARIX LARICINA

1979 ◽  
Vol 111 (2) ◽  
pp. 165-169 ◽  
Author(s):  
M. R. Wagner ◽  
T. Ikeda ◽  
D. M. Benjamin ◽  
F. Matsumura
Keyword(s):  
Thin Layer ◽  
Feeding Behavior ◽  
Larix Laricina ◽  
Distilled Water ◽  
Sawfly Larvae ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Short Shoots ◽  
Layer Chromatography ◽  
Preferential Feeding

AbstractLarch sawfly larvae, Pristiphora erichsonii (Hartig), naturally reject single needles of newly elongated shoots and consume only tufted foliage from 1 year or older short shoots of tamarack, Larix laricina (Du Roi) K. Koch. Forest entomologists have recognized this unusual feeding behavior for over a century, but not until now has the mechanism been understood. In the bioassasy of unpurified single needle methanol extracts (concn. 1 g needle/ml), 88.5% of the larvae were feeding on the control end of the bioassay twig (solvent only) after 4 h. This was significantly different from a similar bioassay of tufted foliage extract (t-value significant at 0.02 level) and a non-treated control bioassay (distilled water on both ends of bioassay twig) (t-value significant at 0.01 level). In the bioassay of a purified extract of single tamarack needles (TLC fraction 7), 81% of larvae were feeding on the untreated end (t-value significant 0.02). Thin-layer chromatography fraction 7, of eight fractions delineated, alone induced significant feeding inhibition.

Dynamics of eastern larch stands and its relationships with larch sawfly outbreaks in the northern Clay Belt of Quebec

2002 ◽  
Vol 32 (2) ◽  
pp. 206-216 ◽  
Author(s):  
Martin-Philippe Girardin ◽  
Jacques Tardif ◽  
Yves Bergeron
Keyword(s):  
Black Spruce ◽  
Critical Factor ◽  
Stand Dynamics ◽  
Larix Laricina ◽  
Gap Dynamics ◽  
Stem Analysis ◽  
Breast Height ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly ◽  
Growth Analyses

Climate change, fires, and insects outbreaks can affect eastern larch (Larix laricina (Du Roi) K. Koch) stand dynamics. To determine which of these factors had the greatest influence on stand dynamics, we sampled four wetlands dominated by larch on the margin of Lake Duparquet in the northern Clay Belt of Quebec. The ages of seedlings, saplings, and trees were determined in twelve 400-m2 plots. Increment cores were taken at breast height to determine past disturbance episodes related, among others, to larch sawfly (Pristiphora erichsonii (Hartig)) activity. Stem analysis was conducted using larch and black spruce (Picea mariana (Mill.) BSP) for identification of post-disturbance releases in height growth. Analyses at the stand and cluster levels showed that larch age structures were characterized by many unsynchronized establishment periods. In addition, a seedlings bank not much older than 10 years characterized many plots. Two larch sawfly outbreak episodes (1895–1912 and 1955–1962) identified by tree-ring and stem analysis were associated with larch establishment. A smaller outbreak in the late 1970s could also have contributed to the initiation of establishment in one stand. Our results suggest that the length (severity) of an outbreak may be a critical factor in explaining the different patterns of establishment observed in these stands. During a severe outbreak, larch establishment may occur mainly from surviving stems (increased seed production), whereas during a mild outbreak, larch establishment may occur from increased survival of pre-established seedlings and saplings. Further studies on the distribution of gaps within larch stands may provide better information on the pattern of mortality (gap dynamics) during an outbreak and may help to better understand larch establishment in these stands.

A Note on the Occurrence of the Larch Sawfly in Alaska

1966 ◽  
Vol 98 (6) ◽  
pp. 671-672
Author(s):  
Donald C. Schmiege
Keyword(s):  
First Record ◽  
Larix Laricina ◽  
Sawfly Larvae ◽  
Pristiphora Erichsonii ◽  
Larch Sawfly

Larvae of the larch sawfly, Pristiphora erichsonii (Htg.), were collected in Alaska in 1965. This is the first record of this insect in Alaska. On 26 and 27 July 1965, stands of tamarack, Larix laricina (Du Roi) K. Koch, were examined at 16 locations along the Richardson Highway southeast of Fairbanks. Larch sawfly larvae were collected from 12 of the stands examined. Collection locations are shown in Fig. 1.

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