scholarly journals Calcium-Ion Incorporation into Titanium Surfaces Accompanied by Electrochemical Apatite-Deposition.

2002 ◽  
Vol 21 (4) ◽  
pp. 306-313 ◽  
Author(s):  
Seiji BAN ◽  
Akira KAMIYA ◽  
Tsutomu SONODA
Keyword(s):  
Calcium Ion ◽  
Titanium Surfaces ◽  
Apatite Deposition ◽  
Ion Incorporation

Apatite deposition on titanium surfaces — the role of albumin adsorption

Biomaterials ◽  
1997 ◽  
Vol 18 (14) ◽  
pp. 963-968 ◽  
Author(s):  
A.P. Serro ◽  
A.C. Fernandes ◽  
B. Saramago ◽  
J. Lima ◽  
M.A. Barbosa
Keyword(s):  
Titanium Surfaces ◽  
Apatite Deposition ◽  
Albumin Adsorption

Bioactivity and Mechanical Property of Starch-Based Organic-Inorganic Hybrids

2007 ◽  
Vol 330-332 ◽  
pp. 439-442
Author(s):  
Toshiki Miyazaki ◽  
S. Yasunaga ◽  
Eiichi Ishida ◽  
Masahiro Ashizuka ◽  
Chikara Ohtsuki
Keyword(s):  
Mechanical Properties ◽  
Calcium Ion ◽  
Silanol Group ◽  
The Body ◽  
Cross Linking ◽  
Bioactive Materials ◽  
Human Cancellous Bone ◽  
Living Bone ◽  
Apatite Deposition ◽  
High Flexibility

So-called bioactive ceramics bond to living bone through the apatite layer formed on their surfaces in the body. The apatite deposition is triggered by dissolution of calcium ion (Ca2+) and by silanol (Si-OH) group formed on the surfaces of the ceramics. It is expected that organic modification of these components would produce bioactive materials with high flexibility. In this study, we examined bioactivity and mechanical properties of the organic-inorganic hybrids from starch by modification with silanol group and calcium ion. Effect of cross-linking agent was also investigated. The obtained hybrids showed bioactivity and mechanical properties analogous to those of human cancellous bone by appropriate control in their compositions. Addition of cross-linking agent to improve mechanical strength of the hybrids did not decrease their bioactivity.

Effects of calcium ion incorporation on bone healing of Ti6Al4V alloy implants in rabbit tibiae

Biomaterials ◽  
2007 ◽  
Vol 28 (22) ◽  
pp. 3306-3313 ◽  
Author(s):  
Jin-Woo Park ◽  
Kwang-Bum Park ◽  
Jo-Young Suh
Keyword(s):  
Bone Healing ◽  
Calcium Ion ◽  
Ti6al4v Alloy ◽  
Ion Incorporation

Towards Enhanced Bioactivity: Calcium Ion-Doped Polypyrrole

2014 ◽  
Vol 941-944 ◽  
pp. 1168-1173
Author(s):  
Yuan He ◽  
Ling Feng Dai ◽  
Shi Hui Wang ◽  
Ya Nan Sun ◽  
Wei Shi ◽  
...  
Keyword(s):  
Calcium Ion ◽  
Ion Concentration ◽  
Bone Tissue Regeneration ◽  
Human Blood Plasma ◽  
Tissue Engineering Scaffolds ◽  
Electrically Conductive ◽  
Bioactive Coating ◽  
Apatite Deposition ◽  
Biomimetic Method ◽  
Bonelike Apatite

Based on the unique redox property of electrically conductive polymers, Ca2+ was incorporated into polypyrrole (PPy) film that previously doped with polyelectrolyte heparin. Then the apatite-forming ability of the Ca2+-doped PPy was examined by a biomimetic method using stimulated body fluid (SBF), which has ion concentration nearly equal to those of human blood plasma. It was found that the Ca2+-doped PPy successfully formed bonelike apatite deposition on its surface after soaking in SBF for only 3 days, whereas the similar apatite deposition was formed on Ca2+-free PPy after soaking in SBF for 7 days. These indicated that the entrapment of Ca2+ into PPy could accelerate the formation of apatite deposition and the Ca2+-doped PPy was possessed of enhanced bioactivity. It is expected that the Ca2+-doped PPy would be a useful bioactive coating material of metallic medical devices or tissue engineering scaffolds to promote the bone tissue regeneration.

Apatite deposition on thermally and anodically oxidized titanium surfaces in a simulated body fluid

Biomaterials ◽  
2003 ◽  
Vol 24 (25) ◽  
pp. 4631-4637 ◽  
Author(s):  
Xiao-Xiang Wang ◽  
Wei Yan ◽  
Satoshi Hayakawa ◽  
Kanji Tsuru ◽  
Akiyoshi Osaka
Keyword(s):  
Simulated Body Fluid ◽  
Body Fluid ◽  
Titanium Surfaces ◽  
Apatite Deposition

Lead aspartate: a new en bloc stain for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 34-35
Author(s):  
J.R. Walton
Keyword(s):  
Electron Microscopy ◽  
Calcium Ion ◽  
Enzyme Reaction ◽  
Lead Citrate ◽  
Reaction Products ◽  
En Bloc ◽  
Thin Sections ◽  
Lead Salts ◽  
Thin Sectioning ◽  
High Alkalinity

In electron microscopy, lead is the metal most widely used for enhancing specimen contrast. Lead citrate requires a pH of 12 to stain thin sections of epoxy-embedded material rapidly and intensively. However, this high alkalinity tends to leach out enzyme reaction products, making lead citrate unsuitable for many cytochemical studies. Substitution of the chelator aspartate for citrate allows staining to be carried out at pH 6 or 7 without apparent effect on cytochemical products. Moreover, due to the low, controlled level of free lead ions, contamination-free staining can be carried out en bloc, prior to dehydration and embedding. En bloc use of lead aspartate permits the grid-staining step to be bypassed, allowing samples to be examined immediately after thin-sectioning.Procedures. To prevent precipitation of lead salts, double- or glass-distilled H20 used in the stain and rinses should be boiled to drive off carbon dioxide and glassware should be carefully rinsed to remove any persisting traces of calcium ion.

Attachment of oral bacteria to titanium surfaces: An SEM study

1994 ◽  
Vol 52 ◽  
pp. 468-469
Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan
Keyword(s):  
Technical Problem ◽  
Titanium Implant ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Thin Sections ◽  
Surface Ultrastructure ◽  
Sem Study ◽  
Key Factor ◽  
Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

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