Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces

2008 ◽  
Vol 86A (1) ◽  
pp. 117-126 ◽  
Author(s):  
Jin-Woo Park ◽  
Jo-Young Suh ◽  
Hyun-Ju Chung
Keyword(s):  
Gene Expression ◽  
Calcium Ion ◽  
Titanium Surfaces ◽  
Ion Incorporation ◽  
Cells Cultured

Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray

2007 ◽  
Vol 361-363 ◽  
pp. 1091-1094
Author(s):  
S.H. Park ◽  
J.M. Park ◽  
H.S. Lee ◽  
K.S. Min ◽  
A.R. Pae ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cdna Microarray ◽  
Titanium Surface ◽  
Control Groups ◽  
Machined Surface ◽  
Implant Surface ◽  
Titanium Surfaces ◽  
Porous Oxide ◽  
Anodized Titanium ◽  
Cells Cultured

One of the techniques for modifying Ti implant surface is an anodization. This method is an electrochemical one that forms rough, thick, and porous oxide films. To compare the gene expression from the cells cultured on the different implant surface, cDNA microarray have been performed. MC3T3-E1 cells were cultured on titanium disks. The control groups were cultured on the machined surfaces(MS). The experimental groups cultured on the anodized titanium surfaces(AS). Microarray assay 48 hours after culturing the cells on the machined surface and anodized surface revealed that osteoinductive molecules appeared more prominent on the anodized surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than anodized surface, which will affect the phenotype of the plated cells depending on the surface morphology.

ROLE OF rhBMP-2 AND rhBMP-7 IN THE METABOLISM AND DIFFERENTIATION OF OSTEOBLAST-LIKE CELLS CULTURED ON CHEMICALLY MODIFIED TITANIUM SURFACES

2012 ◽  
pp. 141208072802005
Author(s):  
Fabiano Ribeiro Cirano ◽  
ADRIANE TOGASHI ◽  
MARCIA MARQUES ◽  
FRANCISCO PUSTIGLIONI ◽  
LUIZ LIMA
Keyword(s):  
Chemically Modified ◽  
Titanium Surfaces ◽  
Cells Cultured

scholarly journals Adhesion Behaviour of Primary Human Osteoblasts and Fibroblasts on Polyether Ether Ketone Compared with Titanium under In Vitro Lipopolysaccharide Incubation

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2739 ◽  
Author(s):  
Korbinian Benz ◽  
Andreas Schöbel ◽  
Marisa Dietz ◽  
Peter Maurer ◽  
Jochen Jackowski
Keyword(s):  
Gene Expression ◽  
Protein Level ◽  
Binding Protein ◽  
Sem Images ◽  
Human Osteoblasts ◽  
Polyether Ether Ketone ◽  
Primary Human Osteoblasts ◽  
Titanium Surfaces ◽  
Ether Ketone

The aim of this in vitro pilot study was to analyse the adhesion behaviour of human osteoblasts and fibroblasts on polyether ether ketone (PEEK) when compared with titanium surfaces in an inflammatory environment under lipopolysaccharide (LPS) incubation. Scanning electron microscopy (SEM) images of primary human osteoblasts/fibroblasts on titanium/PEEK samples were created. The gene expression of the LPS-binding protein (LBP) and the LPS receptor (toll-like receptor 4; TLR4) was measured by real-time polymerase chain reaction (PCR). Immunocytochemistry was used to obtain evidence for the distribution of LBP/TLR4 at the protein level of the extra-cellular-matrix-binding protein vinculin and the actin cytoskeleton. SEM images revealed that the osteoblasts and fibroblasts on the PEEK surfaces had adhesion characteristics comparable to those of titanium. The osteoblasts contracted under LPS incubation and a significantly increased LBP gene expression were detected. This was discernible at the protein level on all the materials. Whereas no increase of TLR4 was detected with regard to mRNA concentrations, a considerable increase in the antibody reaction was detected on all the materials. As is the case with titanium, the colonisation of human osteoblasts and fibroblasts on PEEK samples is possible under pro-inflammatory environmental conditions and the cellular inflammation behaviour towards PEEK is lower than that of titanium.

scholarly journals Gene expression profiling of bone cells on smooth and rough titanium surfaces

2004 ◽  
Vol 15 (11) ◽  
pp. 1255-1258 ◽  
Author(s):  
J. Harle ◽  
V. Salih ◽  
I. Olsen ◽  
P. Brett ◽  
F. Jones ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Bone Cells ◽  
Titanium Surfaces

The influence of titanium surfaces treated by alkalis on macrophage and osteoblast-like cell adhesion and gene expression in vitro

RSC Advances ◽  
2015 ◽  
Vol 5 (99) ◽  
pp. 81378-81387 ◽  
Author(s):  
Ting Ma ◽  
Xi-Yuan Ge ◽  
Sheng-Nan Jia ◽  
Xi Jiang ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alkaline Phosphatase ◽  
Cell Adhesion ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Related Gene ◽  
Titanium Surfaces

The effect of alkali-treated titanium surfaces on inflammation-related gene expression of macrophages and alkaline phosphatase activity of osteoblast-like cells.

Evaluation of the Effect of 1,3-Bis(4-Phenyl)-1H-1,2,3-Triazolyl-2-Propanolol on Gene Expression Levels of JAK2–STAT3, NF-κB, and SOCS3 in Cells Cultured from Biopsies of Mammary Lesions

2015 ◽  
Vol 53 (11-12) ◽  
pp. 291-300 ◽  
Author(s):  
J. L. Malvaez Becerril ◽  
J. G. Santillán Benítez ◽  
J. J. Torres Juárez ◽  
J. M. González Bañales ◽  
H. Mendieta Zerón ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Levels ◽  
Gene Expression Levels ◽  
Cells Cultured ◽  
In Cells
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