scholarly journals Enhancement of lipid productivity from a promising oleaginous fungus Aspergillus sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification

2020 ◽  
Vol 71 (3) ◽  
pp. 371
Author(s):  
E. M. Abdellah ◽  
T. H. Ali ◽  
D. A.M. Abdou ◽  
N. M. Hassanein ◽  
M. Fadel ◽  
...  
Keyword(s):  
Unsaturated Fatty Acids ◽  
Incubation Temperature ◽  
Inorganic Nitrogen ◽  
Lipid Production ◽  
Culture Conditions ◽  
Production Optimization ◽  
Biodiesel Production ◽  
Dry Cell Weight ◽  
Oleaginous Fungus ◽  
Optimization Of Culture Conditions

Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumula­tion increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (15%)) and unsaturated fatty acids in different proportions (mainly linoleic acid C18:2 (24.4%), oleica cid C18:1 (14%) and arachidonic C20:4 (7.4%). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and for the preparation of biodiesel.

scholarly journals Consortium Growth of Filamentous Fungi and Microalgae: Evaluation of Different Cultivation Strategies to Optimize Cell Harvesting and Lipid Accumulation

Energies ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3648
Author(s):  
Savienne M. F. E. Zorn ◽  
Cristiano E. R. Reis ◽  
Messias B. Silva ◽  
Bo Hu ◽  
Heizir F. De Castro
Keyword(s):  
Lipid Accumulation ◽  
Unsaturated Fatty Acids ◽  
Lipid Production ◽  
Synthetic Medium ◽  
Fungal Spores ◽  
Dry Weight ◽  
Biodiesel Production ◽  
Biomass Composition ◽  
Fungal Mycelium ◽  
Fourfold Increase

This study aims to evaluate the potential of consortium biomass formation between Mucor circinelloides, an oleaginous filamentous fungal species, and Chlorella vulgaris, in order to promote a straightforward approach to harvest microalgal cells and to evaluate the lipid production in the consortium system. A synthetic medium with glucose (2 g·L−1) and mineral nutrients essential for both fungi and algae was selected. Four different inoculation strategies were assessed, considering the effect of simultaneous vs. separate development of fungal spores and algae cells, and the presence of a supporting matrix aiming at the higher recovery of algae cell rates. The results were evaluated in terms of consortium biomass composition, demonstrating that the strategy using a mature fungal mycelium with a higher algae count may provide biomass samples with up to 79% of their dry weight as algae, still promoting recovery rates greater than 97%. The findings demonstrate a synergistic effect on the lipid accumulation by the fungal strain, at around a fourfold increase when compared to the axenic control, with values in the range of 23% of dry biomass weight. Furthermore, the fatty acid profile from the samples presents a balance between saturated and unsaturated fatty acids that is likely to present an adequate balance for applications such as biodiesel production.

Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Tian-Hua Zhong ◽  
Jia-Wei Zhang ◽  
Wei Xu ◽  
Liying Huang ◽  
Zhu-Hua Luo
Keyword(s):  
Cell Growth ◽  
Lipid Accumulation ◽  
Oil Production ◽  
Culture Conditions ◽  
Medium Composition ◽  
Open Ocean ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Fermentation Conditions ◽  
Oleaginous Fungus

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

scholarly journals Studies on Optimization of Culture Conditions and Medium Components for the Production of Mycelial Biomass of Auricularia delicata under Submerged Fermentation

2020 ◽  
pp. 56-67
Author(s):  
Muharagi Samwel Jacob ◽  
Li Xiao ◽  
Mabagala Frank Stephano ◽  
Xu Anran
Keyword(s):  
Submerged Fermentation ◽  
Incubation Temperature ◽  
Mineral Elements ◽  
Culture Conditions ◽  
Ministry Of Education ◽  
Mycelial Biomass ◽  
Engineering Research ◽  
Medium Components ◽  
Medicinal Fungi ◽  
Optimization Of Culture Conditions

Aims: To optimize the culture conditions and medium components for the production of mycelial biomass of A. delicata under submerged fermentation. Place and Duration of Study: China–Zambia Agricultural demonstration center and Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China between July 2019 and June 2020. Methodology: In this study, a single factor at a time method was employed in the optimization of submerged culture conditions and medium components for the production of mycelial biomass of A. delicata (strain YD 99). Each factor was screened independently while other factors were kept constant. Results: The findings of this study demonstrate that the optimal culture conditions obtained were as follows: carbon source (Glucose) 20 gl-1, pH 6.0, nitrogen source (Yeast extract) 2 gl-1, mineral elements (K2HPO4+MgSO4.7H20) 2gl-1, and incubation temperature 25°C. The application of these optimal culture conditions produced a maximum concentration of 7.34gl-1 mycelial biomass of A. delicata. Conclusion: Consequently, our results indicated that the optimization of culture conditions and medium components is of significant importance for the cultivation of A. delicata.

scholarly journals Production of chitosan and lipids from a newly isolated Mucor circinelloides

2018 ◽  
Author(s):  
◽  
Johnson Tungamirai Zininga
Keyword(s):  
Sunflower Oil ◽  
Unsaturated Fatty Acids ◽  
Lipid Production ◽  
Cetane Number ◽  
Antimicrobial Properties ◽  
Mucor Circinelloides ◽  
Acid Number ◽  
Biodiesel Production ◽  
Oleaginous Fungi ◽  
Enhanced Production

Filamentous fungi are well-known sources of a wide variety of industrially-useful biomolecules. This study demonstrates the applicability of a newly isolated oleaginous fungi Mucor circinelloides ZSKP for lipid and chitosan production. Parameters affecting co-production were identified and were statistically optimized, which resulted in a 3–fold improvement in lipid production. The lipid profile showed a high content of unsaturated fatty acids including oleic, linolenic and linoleic acids, while palmitic acid was the major saturated fatty acid (21%). A comparative study to evaluate the efficacy of enzymatic and chemical treatments for biodiesel production from fungal lipids and sunflower oil revealed slightly enhanced production of biodiesel from fungal lipids, using a commercial lipase. The biodiesel synthesized using lipids from M. circinelloides ZSKP satisfied standard specifications and had a higher cetane number (56), lower kinematic viscosity (4.6 mm2/s) and lower acid number (0.03) compared to sunflower oil. Upon optimizing chitosan production and extraction processes the chitosan production was improved 2-fold. The fungal chitosan showed antimicrobial properties and was more effective against Aspergillus niger A chitosan spray was developed which was able to increase the shelf life of fresh fruit produce. These results indicate that Mucor circinelloides ZSKP is a promising candidate for concurrent production of lipids and the versatile bio-polymer chitosan.

scholarly journals Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures

2020 ◽  
Vol 8 (7) ◽  
pp. 1054 ◽  
Author(s):  
Erdem Carsanba ◽  
Seraphim Papanikolaou ◽  
Patrick Fickers ◽  
Huseyin Erten
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Culture Conditions ◽  
Microbial Lipid ◽  
Culture Collections ◽  
Batch Cultures ◽  
Food Biotechnology ◽  
High Lipid ◽  
Dry Cell Weight

Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.

scholarly journals Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kukkala Kiran Kumar ◽  
Farha Deeba ◽  
Sauraj ◽  
Yuvraj Singh Negi ◽  
Naseem A. Gaur
Keyword(s):  
High Throughput Screening ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cetane Number ◽  
Nile Red ◽  
Major Fatty Acid ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Dry Cell Weight ◽  
Pre Treatment

Abstract Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

scholarly journals Potential Assessment of Oleaginous Fungi for Sustainable Biodiesel Production: Screening, Identification and Lipid Production Optimization

2020 ◽  
Author(s):  
Ghada Youssef ◽  
Ahmed Elrefaey ◽  
Samy El-Assar
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Aspergillus Terreus ◽  
Total Concentration ◽  
Lipid Production ◽  
Production Optimization ◽  
Biodiesel Production ◽  
Rrna Gene ◽  
Gas Chromatograph ◽  
Oleaginous Fungi

Abstract The present work, aiming to exploit oleaginous fungi for biodiesel production. Ten fungal strains were isolated from two petroleum polluted soil samples and screened for their abilities to accumulate lipid. Lipid rich three species viz, Aspergillus terreus, Aspergillus niger and Aspergillus flavus were found to be the highest lipid producers. Potential isolates were identified at the species level by morphological (macroscopic and microscopic) examination and molecularly confirmed by using 18S rRNA gene sequencing. Improvement of lipid accumulation by optimization of various parameters of culture conditions. The results reported clearly that the most suitable medium conditions for highest lipid production (38.33%) of Aspergillus terreus as the most potent lipid producer composed of 5% sucrose, 0.5 g/L ammonium nitrate with initial pH 6.0, after seven days of incubation in a static condition. The three promising fungal isolates have been taken for fatty acids analysis by gas chromatograph (GC) after transesterification. Fatty acid methyl esters (FAME) profile indicated the presence of higher saturated fatty acid fractions compared to polyunsaturated fatty acids. The total concentration of fatty acids was 107.98, 38.29, and 37.48 mg/100g of lipid accumulated by A. terreus, A. niger and A. flavus, respectively. Gas chromatograph analysis of A. terreus lipid indicated that oleic acid (C18:1, 18.51%) was the most abundant fatty acid, followed by stearic acid (C18:0, 15.91%) and Myristic acid (C14:0, 14.64%), respectively. Therefore, fatty acid profile of A. terreus has confirmed its potentiality as feedstock for producing lipid for biodiesel manufacturing.

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