scholarly journals Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures

2020 ◽  
Vol 8 (7) ◽  
pp. 1054 ◽  
Author(s):  
Erdem Carsanba ◽  
Seraphim Papanikolaou ◽  
Patrick Fickers ◽  
Huseyin Erten
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Culture Conditions ◽  
Microbial Lipid ◽  
Culture Collections ◽  
Batch Cultures ◽  
Food Biotechnology ◽  
High Lipid ◽  
Dry Cell Weight

Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.

scholarly journals Enhancement of gamma-linolenic acid production by the fungus Mucor sp LB-54 by growth temperature

1999 ◽  
Vol 30 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Patrícia de Oliveira Carvalho ◽  
Joaquim Gilberto de Oliveira ◽  
Gláucia Maria Pastore
Keyword(s):  
Fatty Acids ◽  
Growth Temperature ◽  
Lipid Production ◽  
Basal Medium ◽  
Culture Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Glucose Addition ◽  
Dry Cell Weight ◽  
Total Fatty Acids ◽  
Maximal Production

As a relatively prolific producer of GLA, the strain of Mucor sp LB-54 was selected for a study at different growth temperatures in shaker flask culture. The strain used in our experiment was capable to accumulate a relatively high amount of intracellular lipid, 20.73 % of dry cell weight, and GLA content of 15 % of total fatty acids after 5 days of incubation at 28°C. As the growth temperature was decreased from 28 to 12°C the percentage of GLA increased from 15 to 24 % of total fatty acids. In order to optimize the culture conditions for rapid biomass production and lipid production with a high proportion of GLA, the fungus was grown at two temperature combinations associated supplies of carbon source (glucose) in the culture medium. Maximal production of GLA (74 mg/l) was obtained from the Mucor sp LB-54 strain after 5 days of incubation at 28°C in basal medium following glucose addition (7 % w/v) and incubation for an additional 3 days at 12°C. The identity of GLA found in the strain of Mucor sp LB-54 was confirmed by the coupled gas chromatography-mass spectrometry

scholarly journals Aeration and Stirring in Yarrowia lipolytica Lipase Biosynthesis during Batch Cultures with Waste Fish Oil as a Carbon Source

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 88
Author(s):  
Paulina Snopek ◽  
Dorota Nowak ◽  
Bartłomiej Zieniuk ◽  
Agata Fabiszewska
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Lipolytic Activity ◽  
Culture Conditions ◽  
Agitation Speed ◽  
Oxygen Deficit ◽  
Shear Stresses ◽  
Process Selection ◽  
Batch Cultures

Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.

Harnessing the Effect of pH on Lipid Production in Batch Cultures of Yarrowia lipolytica SKY7

2017 ◽  
Vol 184 (4) ◽  
pp. 1332-1346 ◽  
Author(s):  
Mathiazhakan Kuttiraja ◽  
Ayed Dhouha ◽  
Rajeshwar Dayal Tyagi
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Effect Of Ph ◽  
Batch Cultures

scholarly journals Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ruiling Gao ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Wenjun Bao ◽  
Shikun Cheng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Yarrowia Lipolytica ◽  
Butyric Acid ◽  
Lipid Content ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Initial Ph ◽  
Alkaline Conditions

Abstract Background Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30–110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that YX/S and YL/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (YX/S: 0.414) and 3.20 g/L (YL/S: 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (YX/S: 0.534) and 3.08 g/L (YL/S: 0.139), respectively, with a lipid content of 26.02%. Conclusions This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid production. Feasibilities on FW and FVW-derived VFAs were evaluated, and meaningful information was provided for practical applications.

scholarly journals Volatile Fatty Acids from Lipid-Extracted Yeast Provide Additional Feedstock for Microbial Lipid Production

Catalysts ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1009
Author(s):  
Gwon Woo Park ◽  
Seongsoo Son ◽  
Myounghoon Moon ◽  
Subin Sin ◽  
Kyoungseon Min ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Response Surface Methodology ◽  
Carbon Source ◽  
Response Surface ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Initial Ph ◽  
Microbial Lipid Production

Microbial lipid production from oleaginous yeasts is a promising process for the sustainable development of the microbial biodiesel industry. However, the feedstock cost poses an economic problem for the production of microbial biodiesel. After lipid extraction, yeast biomass can be used as an organic source for microbial biodiesel production. In this study, volatile fatty acids (VFAs), produced via anaerobic digestion of a lipid-extracted yeast (LEY) residue, were utilized as a carbon source for the yeast Cryptococcus curvatus. The response surface methodology was used to determine the initial pH and inoculum volume for the optimal VFA production. The experimental result for VFA concentration was 4.51 g/L at an initial pH of 9 and an inoculation 25%. The optimization results from the response surface methodology showed that the maximal VFA concentration was 4.58 g/L at an initial pH of 8.40 and an inoculation of 39.49%. This study indicates that VFAs from LEY can be used as a carbon source for microbial biodiesel production, with the potential to significantly reduce feedstock costs.

scholarly journals Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica

2013 ◽  
Vol 80 (5) ◽  
pp. 1660-1669 ◽  
Author(s):  
Falk Matthäus ◽  
Markus Ketelhot ◽  
Michael Gatter ◽  
Gerold Barth
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Body ◽  
Lipid Bodies ◽  
Content Type ◽  
Batch Cultures ◽  
Dry Cell Weight ◽  
Lycopene Content ◽  
Rate Limiting ◽  
Dry Cell ◽  
Yeast Yarrowia Lipolytica

ABSTRACTThe codon-optimized genescrtBandcrtIofPantoea ananatiswere expressed inYarrowia lipolyticaunder the control of theTEF1promoter ofY. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis inY. lipolytica,GGS1andHMG1, were overexpressed to increase the production of lycopene. All of the genes were also expressed in aY. lipolyticastrain withPOX1toPOX6andGUT2deleted, which led to an increase in the size of lipid bodies and a further increase in lycopene production. Lycopene is located mainly within lipid bodies, and increased lipid body formation leads to an increase in the lycopene storage capacity ofY. lipolytica. Growth-limiting conditions increase the specific lycopene content. Finally, a yield of 16 mg g−1(dry cell weight) was reached in fed-batch cultures, which is the highest value reported so far for a eukaryotic host.

scholarly journals Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Yinghang Liu ◽  
Xin Jiang ◽  
Zhiyong Cui ◽  
Zhaoxuan Wang ◽  
Qingsheng Qi ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Mevalonate Pathway ◽  
Lipid Production ◽  
Economic Value ◽  
Culture Conditions ◽  
End Joining ◽  
Expression Library ◽  
Cofactor Specificity ◽  
Non Homologous End Joining ◽  
Iterative Integration

Abstract Background Yarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of Y. lipolytica for terpenoid production. Results We constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into the Y. lipolytica chromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cofactor specificity. Based on this strain, the downstream α-farnesene synthesis pathway was overexpressed by iterative integration. Culture conditions were also optimized. A strain that produced 25.55 g/L α-farnesene was obtained. This is the highest terpenoid titer reported in Y. lipolytica. Conclusions Yarrowia lipolytica is a potentially valuable species for terpenoid production, and NHEJ-mediated modular integration is effective for expression library construction and screening of high-producer strains.

scholarly journals Studies on Upgradation of Waste Fish Oil to Lipid-Rich Yeast Biomass in Yarrowia lipolytica Batch Cultures

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 436
Author(s):  
Agata Urszula Fabiszewska ◽  
Bartłomiej Zieniuk ◽  
Mariola Kozłowska ◽  
Patrycja Maria Mazurczak-Zieniuk ◽  
Małgorzata Wołoszynowska ◽  
...  
Keyword(s):  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Ph Regulation ◽  
Culture Conditions ◽  
Waste Oil ◽  
Fish Waste ◽  
Batch Cultures ◽  
Yeast Biomass ◽  
Cellular Lipids ◽  
Positive Effect

The aim of the study was to evaluate the possibility to utilize a fish waste oil issued from the industrial smoking process in nitrogen-limited Yarrowia lipolytica yeast batch cultures. The waste carbon source was utilized by the yeast and stimulated the single cell oil production via an ex novo pathway. The yeast biomass contained lipids up to 0.227 g/g d.m.. Independently from culture conditions, high contents of very long chain fatty acids were quantified in yeast biomass including docosahexaenoic (DHA), eicosapentaenoic acid (EPA), eicosenic and erucic acids. The pH regulation did not influence the cellular lipids yield (0.234 g/g d.m.). Meanwhile, the intensification of the oxygenation of medium by changing the mixing speed (maximum concentration of lipids produced 4.64 g/dm3) and decreasing the amount of inoculum had a positive effect on the culture parameters in waste fish oil medium. Further work on upgradation of the original waste is advisable, especially because the oil indicated high content of polyphenols and lower susceptibility to oxidation than microbial oil derived from control olive oil medium.

scholarly journals Influence of different abiotic factors on lipid production by microalgae – a review

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 57
Author(s):  
Clément Gaignard ◽  
George Zissis ◽  
David Buso
Keyword(s):  
Fossil Fuels ◽  
Culture Medium ◽  
Abiotic Factors ◽  
Lipid Production ◽  
Culture Conditions ◽  
Medium Temperature ◽  
High Lipid ◽  
Downstream Processes ◽  
The Cost ◽  
Accumulation Of Lipids

Research on lipid production in microalgae, especially to target biofuels, has been widely published in recent decades. This literature is mostly focused on the optimization of culture conditions, metabolic engineering, biorefinery and downstream processes. At the lab scale, lipid production is very promising concerning biofuels production based on microalgae due to their high lipid content. However, the accumulation of knowledge associated with the development of processes and technologies for the production of biomass and associated lipids has so far not achieved the hoped-for commercial success due to still too expensive operating costs compared to fossil fuels. This review aims to present the influence of various abiotic factors on the accumulation of lipids and lipid profile in microalgae, such as the depletion of the culture medium, temperature and light, but also most innovative like the induction by hormones. Other optimization procedures and the use of wastewater to improve the cost-viability are discussed in perspective.

scholarly journals Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kukkala Kiran Kumar ◽  
Farha Deeba ◽  
Sauraj ◽  
Yuvraj Singh Negi ◽  
Naseem A. Gaur
Keyword(s):  
High Throughput Screening ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cetane Number ◽  
Nile Red ◽  
Major Fatty Acid ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Dry Cell Weight ◽  
Pre Treatment

Abstract Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

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