Evaluating the effect of medium composition and fermentation conditions on microbial oil production by a Fusarium strain isolated from the open ocean

2017 ◽  
Vol 60 (4) ◽  
Author(s):  
Tian-Hua Zhong ◽  
Jia-Wei Zhang ◽  
Wei Xu ◽  
Liying Huang ◽  
Zhu-Hua Luo
Keyword(s):  
Cell Growth ◽  
Lipid Accumulation ◽  
Oil Production ◽  
Culture Conditions ◽  
Medium Composition ◽  
Open Ocean ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Fermentation Conditions ◽  
Oleaginous Fungus

AbstractAs an important feedstock for biodiesel production, microbial oil has attracted considerable interest in recent years. Here, we investigated the effect of medium composition and culture conditions on cell growth and lipid accumulation of an oleaginous fungus

Microbial Oil Production from Lignocellulosic Biomass – Recent Development and Prospect

2014 ◽  
Vol 541-542 ◽  
pp. 397-403
Author(s):  
Zhang Nan Lin ◽  
Hong Juan Liu ◽  
Zhi Qin Wang ◽  
Jia Nan Zhang
Keyword(s):  
Lignocellulosic Biomass ◽  
Large Scale ◽  
Raw Materials ◽  
Oil Production ◽  
Renewable Resource ◽  
Raw Material ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Key Issues ◽  
Application Potential

Microbial oil is one of the ideal raw materials for biodiesel production because of its rapid reproduction and less influence by the climate and season variation. However, the high cost is one of the key issues that restricted its production in a large-scale. Lignocellulosic biomass, the cheap and renewable resource, might be the best raw material for microbial oil production by oleaginous microorganisms. Recent development on the microbial oil production from lignocellulosic biomass was summarized in this paper. Furthermore, the challenges and application potential of microbial oil were prospected.

Microbial oil production from thermophile cyanobacteria for biodiesel production

2011 ◽  
Vol 88 (11) ◽  
pp. 3632-3635 ◽  
Author(s):  
Sevgi Ertuğrul Karatay ◽  
Gönül Dönmez
Keyword(s):  
Oil Production ◽  
Biodiesel Production ◽  
Microbial Oil

scholarly journals Optimization of Oil Production from Cassava Pulp and Sugarcane Bagasse using Oleaginous Yeast

2019 ◽  
Vol 35 (2) ◽  
pp. 668-677 ◽  
Author(s):  
Phakamas Rachamontree ◽  
Malinee Sriariyanun ◽  
Surapun Tepaamorndech ◽  
Issaraporn Somboonwatthanakul
Keyword(s):  
Fatty Acid ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Value Added ◽  
Oil Production ◽  
Biodiesel Production ◽  
Oil Yield ◽  
Microbial Oil ◽  
Crude Lipid ◽  
Cassava Pulp

Microbial oil is one of alternative sources for biofuel and value-added chemical production in biorefining process of lignocellulosic biomass. The bottleneck of this process is the low conversion rate of lignocellulose biomass to the target product. This research aims to optimize microbial oil production as raw material for biorefining process from agricultural waste by using Plackett–Burman (PB) design and Response Surface Methodology (RSM). Acid-hydrolysates of cassava pulp and sugarcane bagasse were converted to bio-oil by fermentation activities of two oleaginous yeasts, designated as MSU2 and Ka28. The fermentation parameters were screened by PB design to identify their impacts on oil yield, and the condition of each parameter was optimized by RSM to maximized oil yield. The predicted optimal condition obtained from RSM provided the highest lipid yield at 34.56 and 21.85% from hydrolysates of cassava pulp and sugarcane bagasse, respectively. Then, fatty acid compositions in oil were analyzed by GCMS, and our results demonstrated that palmitic acid and oleic acid were the major constituents at 72.95 and 195.01 mg/g-crude lipid, respectively. These fatty acid distribution profiles were suitable for application in biodiesel production and suggested the utilization of agricultural waste in biorefining process.

scholarly journals Strategies for increasing lipid accumulation and recovery from Y. lipolytica: A review

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 51
Author(s):  
Sally El Kantar ◽  
Anissa Khelfa ◽  
Eugène Vorobiev ◽  
Mohamed Koubaa
Keyword(s):  
Lipid Accumulation ◽  
Fossil Fuel ◽  
Low Cost ◽  
Operating Cost ◽  
Cost Effective ◽  
Biodiesel Production ◽  
Cultivation Conditions ◽  
Oleaginous Yeasts ◽  
Fermentation Conditions ◽  
High Lipid

Microbial-based biodiesel is produced by transesterification of lipids extracted from microbial cells, and is considered as a potential replacement of fossil fuel due to its advantages in reducing greenhouse gas emissions. Yarrowia lipolytica is one of the most studied oleaginous yeasts able to produce lipids under some fermentation conditions and is considered as a potential industrial host for biodiesel production. Several approaches have been evaluated to increase the economical attraction of biodiesel production from Y. lipolytica lipids. In this review, we highlighted the different strategies reported in the literature, allowing this yeast to achieve high lipid accumulation. These include metabolic engineering strategies, the use of low-cost effective substrates, and the optimization of the cultivation conditions for higher lipid productivity and less operating cost. We also summarized the most effective cell disruption technologies that improve the extraction efficiencies of lipids from Y. lipolytica.

scholarly journals Enhancement of lipid productivity from a promising oleaginous fungus Aspergillus sp. strain EM2018 for biodiesel production: Optimization of culture conditions and identification

2020 ◽  
Vol 71 (3) ◽  
pp. 371
Author(s):  
E. M. Abdellah ◽  
T. H. Ali ◽  
D. A.M. Abdou ◽  
N. M. Hassanein ◽  
M. Fadel ◽  
...  
Keyword(s):  
Unsaturated Fatty Acids ◽  
Incubation Temperature ◽  
Inorganic Nitrogen ◽  
Lipid Production ◽  
Culture Conditions ◽  
Production Optimization ◽  
Biodiesel Production ◽  
Dry Cell Weight ◽  
Oleaginous Fungus ◽  
Optimization Of Culture Conditions

Oleaginous fungi have recently gained increasing attention among different microorganisms due to their ability for lipid production for the preparation of biofuel. In the present study, a locally isolated fungus E45, identified genetically as Aspergillus sp. strain EM2018, was found to produce 25.2% of the total lipids content of its dry cell weight (DCW). Optimization of culture conditions was performed and lipid accumula­tion increased by about 2.4 fold (from 25.2% to 60.1% of DCW) when the fungus was grown for seven days in the potato dextrose (50 g/L) liquid medium at pH 5.0, incubation temperature at 30 ºC and inoculum size of 2 × 106 spore/mL. Supplementation of the medium with yeast extract and NaNO3 at a concentration of 0.05% as organic and inorganic nitrogen sources, respectively, increased lipid production (53.3% lipid/dry biomass). Gas chromatography analysis of fungal lipids revealed the presence of saturated (mainly palmitic acid C16:0 (33%) and lignoceric acid C24:0 (15%)) and unsaturated fatty acids in different proportions (mainly linoleic acid C18:2 (24.4%), oleica cid C18:1 (14%) and arachidonic C20:4 (7.4%). These findings suggest this new oleaginous fungus as a promising feedstock for various industrial applications and for the preparation of biodiesel.

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