scholarly journals Isolation of Peptidolipin NA Derivatives from the Culture of Nocardia arthritidis IFM10035T in the Presence of Mouse Macrophage Cells

Heterocycles ◽  
2022 ◽  
Vol 104 (1) ◽  
pp. 185
Author(s):  
Yasumasa Hara ◽  
Masami Ishibashi ◽  
Daiki Tanimura ◽  
Teruhisa Manome ◽  
Midori A. Arai ◽  
...  
Keyword(s):  
Mouse Macrophage ◽  
Macrophage Cells

Enhancement of the immune response against Salmonella infection of mice by heat-killed multispecies combinations of lactic acid bacteria

2013 ◽  
Vol 62 (11) ◽  
pp. 1657-1664 ◽  
Author(s):  
Chih-Yuan Chen ◽  
Hau-Yang Tsen ◽  
Chun-Li Lin ◽  
Chien-Ku Lin ◽  
Li-Tsen Chuang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Interleukin 12 ◽  
Mouse Serum ◽  
Immunomodulatory Activity ◽  
Mouse Macrophage ◽  
Macrophage Cells ◽  
Tnf Α ◽  
Anti Inflammatory Cytokine ◽  
Pro Inflammatory Cytokines

Heat-killed lactic acid bacteria (LAB) has advantages over live LAB in that it has a long shelf‐life and is therefore easy to store and transport. From four LAB strains selected by immunomodulatory activity and adherent properties, we prepared the heat-killed multispecies combination of LAB (MLAB) and the cell walls from MLAB under two conditions (100 °C for 30 min and 121 °C for 15 min). Different effects on the adherent properties of these four LAB strains were observed, depending on the heating conditions. With mouse macrophage cells, the two heat-killed MLABs (HMLABs) showed significantly higher induction activities on the production of interleukin 12 (IL-12) than their individual strains did. Heat-killed MLABs and cell‐wall preparations were able to reduce the Salmonella invasion of Caco-2 and mouse macrophage cells. Feeding mice with HMLAB could inhibit the Salmonella invasion of mice significantly. For these mice, the expression level of pro-inflammatory cytokines, such as TNF-α and IL-6, in mouse serum was reduced while that of the anti-inflammatory cytokine, i.e. IL-10, was enhanced. The HMLABs developed in this study showed higher protective effect against Salmonella invasion either of Caco-2 cells or of mice, relative to the heat-killed lactobacilli, which consisted of Lactobacillus acidophilus strains selected at random. In conclusion, the HMLABs were potentially useful for the protection of mice against Salmonella infection and the induced inflammation.

ROS generation and DNA damage contribute to abamectin-induced cytotoxicity in mouse macrophage cells

Chemosphere ◽  
2019 ◽  
Vol 234 ◽  
pp. 328-337 ◽  
Author(s):  
Yiran Liang ◽  
Bizhang Dong ◽  
Nannan Pang ◽  
Jiye Hu
Keyword(s):  
Dna Damage ◽  
Ros Generation ◽  
Mouse Macrophage ◽  
Macrophage Cells

Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

2009 ◽  
Vol 31 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Chikatoshi Kasugai ◽  
Akiko Morikawa ◽  
Yoshikazu Naiki ◽  
Naoki Koide ◽  
Takayuki Komatsu ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Macrophage Cells

Different action of IBMX, isoproterenol and rutin on orthovanadate-induced nitric oxide release in mouse macrophage cells

2002 ◽  
Vol 50 (3) ◽  
pp. 323-341
Author(s):  
S. Koncz ◽  
Edit J. Horváth
Keyword(s):  
Nitric Oxide ◽  
Adrenergic Receptor ◽  
No Production ◽  
Mouse Macrophage ◽  
Nitric Oxide Release ◽  
Macrophage Cells ◽  
Reverse Mode ◽  
Dichlorofluorescein Diacetate ◽  
Superoxide Scavenger ◽  
Adrenergic Receptor Agonist

The effects of cAMP-elevating compounds IBMX (3-isobutyl-1-methyl­xanthine) and isoproterenol, and that of rutin (an effective superoxide scavenger) were studied on orthovanadate- (a putative protein-phosphotyrosine phosphatase inhibitor) induced nitric oxide (NO) production in J774A.1 mouse macrophage cells. As we previously reported (Koncz and Horváth, 2000), rutin and sodium orthovanadate act synergistically to induce production of high amount of NO in J774A.1 cells. IBMX, an agent that can elevate cAMP level in the cells, can reduce the production of both the LPS- and rutin + orthovanadate-induced NO in macrophages. In contrast, isoproterenol, a non-selective ß-adrenergic receptor agonist, that reduced the LPS-induced NO production in macrophage cells, was unable to reduce the rutin + orthovanadate-induced NO production without negatively affecting cell viability. Moreover, isoproterenol dramatically enhanced the orthovanadate-induced NO synthesis in J774A.1 cells. Our previous study clarified that rutin and orthovanadate, in a specific concentration ratio of both, were able to produce hydrogen peroxide (H2O2). Using 2',7'-dichlorofluorescein-diacetate as a marker for H2O2, isoproterenol alone induced its oxidation but the rutin plus orthovanadate-induced H2O2 production was reduced by isoproterenol. These observations have revealed that, in some cases, H2O2 and superoxide (O2-) scavengers can act in a reverse mode on macrophage cells depending on the presence or absence of orthovanadate.

Fermented guava leaf extract inhibits LPS-induced COX-2 and iNOS expression in Mouse macrophage cells by inhibition of transcription factor NF-κB

2008 ◽  
Vol 22 (8) ◽  
pp. 1030-1034 ◽  
Author(s):  
Soo-Youn Choi ◽  
Joon-Ho Hwang ◽  
Soo-Young Park ◽  
Yeong-Jun Jin ◽  
Hee-Chul Ko ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leaf Extract ◽  
Inos Expression ◽  
Mouse Macrophage ◽  
Macrophage Cells ◽  
Cox 2 ◽  
Guava Leaf
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