Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells

2009 ◽  
Vol 31 (1) ◽  
pp. 103-107 ◽  
Author(s):  
Chikatoshi Kasugai ◽  
Akiko Morikawa ◽  
Yoshikazu Naiki ◽  
Naoki Koide ◽  
Takayuki Komatsu ◽  
...  
Keyword(s):  
Concanavalin A ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Macrophage Cells

scholarly journals Candida albicans Killing by RAW 264.7 Mouse Macrophage Cells: Effects of Candida Genotype, Infection Ratios, and Gamma Interferon Treatment

2002 ◽  
Vol 70 (11) ◽  
pp. 6319-6329 ◽  
Author(s):  
A. Marcil ◽  
D. Harcus ◽  
D. Y. Thomas ◽  
M. Whiteway
Keyword(s):  
Candida Albicans ◽  
Cell Line ◽  
Systemic Infection ◽  
Gamma Interferon ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Wild Type ◽  
Macrophage Cells ◽  
Dilution Assay ◽  
Infection Models

ABSTRACT Phagocytic cells such as neutrophils and macrophages are potential components of the immune defense that protects mammals against Candida albicans infection. We have tested the interaction between the mouse macrophage cell line RAW 264.7 and a variety of mutant strains of C. albicans. We used an end point dilution assay to monitor the killing of C. albicans at low multiplicities of infection (MOIs). Several mutants that show reduced virulence in mouse systemic-infection models show reduced colony formation in the presence of macrophage cells. To permit analysis of the macrophage-Candida interaction at higher MOIs, we introduced a luciferase reporter gene into wild-type and mutant Candida cells and used loss of the luminescence signal to quantify proliferation. This assay gave results similar to those for the end point dilution assay. Activation of the macrophages with mouse gamma interferon did not enhance anti-Candida activity. Continued coculture of the Candida and macrophage cells eventually led to death of the macrophages, but for the RAW 264.7 cell line this was not due to apoptotic pathways involving caspase-8 or -9 activation. In general Candida cells defective in the formation of hyphae were both less virulent in animal models and more sensitive to macrophage engulfment and growth inhibition. However the nonvirulent, hypha-defective cla4 mutant line was considerably more resistant to macrophage-mediated inhibition than the wild-type strain. Thus although mutants sensitive to engulfment are typically less virulent in systemic-infection models, sensitivity to phagocytic macrophage cells is not the unique determinant of C. albicans virulence.

scholarly journals Effect of artemisinins and other endoperoxides on nitric oxide-related signaling pathway in RAW 264.7 mouse macrophage cells

Nitric Oxide ◽  
2008 ◽  
Vol 19 (2) ◽  
pp. 184-191 ◽  
Author(s):  
V. Badireenath Konkimalla ◽  
Martina Blunder ◽  
Bernhard Korn ◽  
Shahid A. Soomro ◽  
Herwig Jansen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Signaling Pathway ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Macrophage Cells

scholarly journals Anti-Inflammatory Effects of Heat-Processed Artemisia capillaris Thunberg by Regulating IκBα/NF-κB Complex and 15-PGDH in Mouse Macrophage Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Akhtar Ali ◽  
Junsik Lim ◽  
En Hyung Kim ◽  
Jong-Hyun Lee ◽  
Shin Seong ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Heat Processing ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Mouse Macrophage ◽  
Traditional Herbal Medicine ◽  
Inflammatory Disorders ◽  
Artemisia Capillaris ◽  
Macrophage Cells ◽  
Anti Inflammatory

Growing evidence suggests that dietary nutrients in herbs and plants are beneficial in improving inflammatory disorders. Artemisia capillaris Thunberg (AC) is a traditional herbal medicine widely used in East Asia to treat pain, hepatotoxicity, and inflammatory disorders. Heat processing is a unique pharmaceutical method used in traditional herbal medicine to enhance the pharmacological effects and safety of medicinal plants. This study demonstrates the anti-inflammatory effects of heat-processed AC (HPAC) in lipopolysaccharide- (LPS-) treated mouse macrophage cells. HPAC reduced LPS-induced inflammatory mediators such as IL-6, IL-1β, TNF-α, NO, and PGE2 in RAW 264.7 cells. Interestingly, 15-PGDH appears to play a pivotal role rather than COX-2 and mPGES-1 when HPAC regulated PGE2 levels. Meanwhile, HPAC showed anti-inflammatory effects by blocking IκBα phosphorylation and NF-κB nuclear translocalization. Also, we found that HO-1 upregulation was mediated by the mitogen-activated protein kinase (MAPK) pathways in HPAC-treated RAW 264.7 cells. And, in RAW 264.7 cells challenged with LPS, HPAC restored HO-1 expression, leading to NF-κB inhibition. Through further experiments using specific MAPK inhibitors, we found that, in response to LPS, the phosphorylated IκBα and activated NF-κB were attenuated by p38 MAPK/HO-1 pathway. Therefore, HPAC targeting both the IκBα/NF-κB complex and 15-PGDH may be considered as a potential novel anti-inflammatory agent derived from a natural source.

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