Fermented guava leaf extract inhibits LPS-induced COX-2 and iNOS expression in Mouse macrophage cells by inhibition of transcription factor NF-κB

2008 ◽  
Vol 22 (8) ◽  
pp. 1030-1034 ◽  
Author(s):  
Soo-Youn Choi ◽  
Joon-Ho Hwang ◽  
Soo-Young Park ◽  
Yeong-Jun Jin ◽  
Hee-Chul Ko ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leaf Extract ◽  
Inos Expression ◽  
Mouse Macrophage ◽  
Macrophage Cells ◽  
Cox 2 ◽  
Guava Leaf

scholarly journals 1H nuclear magnetic resonance-based metabolite profiling of guava leaf extract: an attempt to develop a prototype for standardization of plant extracts

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Manasi S. Gholkar ◽  
Jia V. Li ◽  
Poonam G. Daswani ◽  
P. Tetali ◽  
Tannaz J. Birdi
Keyword(s):  
Ferulic Acid ◽  
Gallic Acid ◽  
Plant Extracts ◽  
Nmr Spectra ◽  
Leaf Extract ◽  
Partial Least Square ◽  
Least Square ◽  
E Coli ◽  
H Nmr ◽  
Guava Leaf

Abstract Background Herbal medicines are fast gaining popularity. However, their acceptability by modern practitioners is low which is often due to lack of standardization. Several approaches towards standardization of herbals have been employed. The current study attempted to recognize key peaks from 1H NMR spectra which together would comprise of a spectral fingerprint relating to efficacy of Psidium guajava (guava) leaf extract as an antidiarrhoeal when a number of unidentified active principles are involved. Methods Ninety samples of guava leaves were collected from three locations over three seasons. Hydroalcoholic (water and ethanol, 50:50) extracts of these samples were prepared and their 1H NMR spectra were acquired. Spectra were also obtained for quercetin, ferulic acid and gallic acid as standards. Eight bioassays reflecting different stages of diarrhoeal pathogenesis were undertaken and based on pre-decided cut-offs, the extracts were classified as ‘good’ or ‘poor’ extracts. The bioactivity data was then correlated with the 1H NMR profiles using Regression or Orthogonal Partial Least Square-Discriminant Analysis (OPLS-DA). Results OPLS-DA showed seasonal and regional segregation of extracts. Significant models were established for seven bioassays, namely those for anti-bacterial activity against Shigella flexneri and Vibrio cholerae, adherence of E. coli, invasion of E. coli and S. flexneri and production and binding of toxin produced by V. cholerae. It was observed that none of the extracts were good or bad across all the bioassays. The spectral analysis showed multiple peaks correlating with a particular activity. Based on NMR and LC-MS/MS, it was noted that the extracts contained quercetin, ferulic acid and gallic acid. However, they did not correlate with the peaks that segregated extracts with good and poor activity. Conclusions The current study identified key peaks in 1H NMR spectra contributing to the anti-diarrhoeal activity of guava leaf extracts. The approach of using spectral fingerprinting employed in the present study can thus be used as a prototype towards standardization of plant extracts with respect to efficacy.

Red-fluorescent graphene quantum dots from guava leaf as a turn-off probe for sensing aqueous Hg(ii)

2021 ◽  
Vol 45 (10) ◽  
pp. 4617-4625
Author(s):  
Rahul V. Khose ◽  
Goutam Chakraborty ◽  
Mahesh P. Bondarde ◽  
Pravin H. Wadekar ◽  
Alok K. Ray ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Metal Ion ◽  
Leaf Extract ◽  
Graphene Quantum Dots ◽  
Toxic Metal ◽  
Guava Leaf

In this work, we have prepared red-fluorescent graphene quantum dots and utilized as a highly selective and sensitive fluorescence turn-off probe for detection of the toxic metal ion Hg2+ from guava leaf extract.

scholarly journals Anti-hyperglycemic and anti-hyperlipidemic effects of guava leaf extract

2010 ◽  
Vol 7 (1) ◽  
pp. 9 ◽  
Author(s):  
Yoriko Deguchi ◽  
Kouji Miyazaki
Keyword(s):  
Leaf Extract ◽  
Guava Leaf

scholarly journals Pseudane-VII Regulates LPS-Induced Neuroinflammation in Brain Microglia Cells through the Inhibition of iNOS Expression

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3196 ◽  
Author(s):  
Mi Kim ◽  
Inae Jung ◽  
Ju Na ◽  
Yujeong Lee ◽  
Jaewon Lee ◽  
...  
Keyword(s):  
Neurodegenerative Diseases ◽  
Inflammatory Responses ◽  
Neurological Diseases ◽  
Inos Expression ◽  
Nuclear Factor Kappab ◽  
Nuclear Factor ◽  
Ros Production ◽  
Proinflammatory Mediators ◽  
Cox 2 ◽  
Novel Target

We previously isolated pseudane-VII from the secondary metabolites of Pseudoalteromonas sp. M2 in marine water, and demonstrated its anti-inflammatory efficacy on macrophages. However, the molecular mechanism by which pseudane-VII suppresses neuroinflammation has not yet been elucidated in brain microglia. Microglia is activated by immunological stimulation or brain injury. Activated microglia secrete proinflammatory mediators which damage neurons. Neuroinflammation appears to be associated with certain neurological diseases, including Parkinson’s disease and Alzheimer’s disease. Natural compounds that suppress microglial inflammatory responses could potentially be used to prevent neurodegenerative diseases or slow their progression. In the present study, we found that pseudane-VII suppresses neuroinflammation in lipopolysaccaride (LPS)-stimulated BV-2 microglial cells and brain. Pseudane-VII was shown to inhibit the LPS-stimulated NO, ROS production and the expression of iNOS and COX-2. To identify the signaling pathway targeted by pseudane-VII, we used western blot analysis to assess the LPS-induced phosphorylation state of p38, ERK1/2, JNK1/2, and nuclear factor-kappaB (NF-κB). We found that pseudane-VII attenuated LPS-induced phosphorylation of MAPK and NF-κB. Moreover, administration of pseudane-VII in mice significantly reduced LPS-induced iNOS expression and microglia activation in brain. Taken together, our findings suggest that pseudane-VII may represent a potential novel target for treatment for neurodegenerative diseases.

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