scholarly journals Molecular Characterization of Fasciola hepatica Infecting Cattle from Egypt Based on Mitochondrial and Nuclear Ribosomal DNA Sequences

2016 ◽  
Vol 11 (3) ◽  
pp. 61-66 ◽  
Author(s):  
Sherif M. Nasr ◽  
Eman K.A. Bazh ◽  
Reda S. Fadly
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Fasciola Hepatica ◽  
Nuclear Ribosomal Dna

Molecular characterization of Fasciola gigantica from Mauritania based on mitochondrial and nuclear ribosomal DNA sequences

2011 ◽  
Vol 129 (2) ◽  
pp. 127-136 ◽  
Author(s):  
Nabil Amor ◽  
Sarra Farjallah ◽  
Mohamed Salem ◽  
Dia Mamadou Lamine ◽  
Paolo Merella ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Fasciola Gigantica ◽  
Nuclear Ribosomal Dna

Molecular characterization of Fasciola spp. from the endemic area of northern Iran based on nuclear ribosomal DNA sequences

2011 ◽  
Vol 128 (3) ◽  
pp. 196-204 ◽  
Author(s):  
Nabil Amor ◽  
Ali Halajian ◽  
Sarra Farjallah ◽  
Paolo Merella ◽  
Khaled Said ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Endemic Area ◽  
Nuclear Ribosomal Dna ◽  
Northern Iran ◽  
Fasciola Spp

Reviewing lymnaeid vectors of fascioliasis by ribosomal DNA sequence analyses

2005 ◽  
Vol 79 (3) ◽  
pp. 257-267 ◽  
Author(s):  
M.D. Bargues ◽  
S. Mas-Coma
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Fasciola Hepatica ◽  
Rdna Sequence ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Nuclear Rdna ◽  
Natural Classification ◽  
The Family

AbstractSnails of the family Lymnaeidae are of great parasitological importance due to the numerous helminth species they transmit, mainly trematodiases (such as fascioliasis) of considerable medical and veterinary impact. The present knowledge of the genetics and host–parasite relationships of this gastropod group is far from adequate. Fascioliasis is caused by two species, Fasciola hepatica and F. gigantica, which, as in the case of other trematodes, show a marked snail host specificity. Many lymnaeid species involved in fascioliasis transmission still show a confused systematic-taxonomic status. The need for tools to distinguish and characterize species and populations of lymnaeids is evident and the present review concerns new molecular tools developed in recent years using nuclear ribosomal DNA sequences. The small subunit or 18S gene and the internal transcribed spacers ITS-2 and ITS-1 are analysed and evaluated as markers for taxon differentiation and relationships within the Lymnaeidae from genus and species levels to subspecies and population levels. rDNA sequence differences and genetic distances, and their value for reconstructing phylogenetic trees using different methods are considered. Nuclear rDNA sequences are appropriate tools on which to base a review of the systematics and taxonomy of the family Lymnaeidae, without excluding other valuable snail characteristics already available. A reconstruction of the lymnaeid system towards a more natural classification will undoubtedly be helpful in understanding parasite transmission and epidemiological features as well the dispersion of an emerging-reemerging disease such as fascioliasis. Nomenclature for nuclear rDNA genotyping in lymnaeids includes the main rDNA sequence regions able to furnish important information on interspecific differentiation and grouping as well as intraspecific variability of lymnaeid species. The composite haplotype code includes the rDNA markers arranged in order according to their well-known usefulness, in its turn related to their respective, more or less rapid evolutionary ratios, to distinguish between different taxonomic levels, from supraspecific taxa to the species level and up to the population level.

MOLECULAR CHARACTERIZATION OF Dunaliella salina AND Chlorella vulgaris FUSANT USING 18SrDNA GENE

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Hermin Pancasakti Kusumaningrum ◽  
Muhammad Zainuri
Keyword(s):  
Quantitative Analysis ◽  
Molecular Characterization ◽  
Ribosomal Dna ◽  
Efficient Method ◽  
Dna Sequences ◽  
Dunaliella Salina ◽  
Parental Genome ◽  
Carotenoid Production ◽  
Relationship Of

Protoplast fusion was found to be an efficient method in improving carotenoid production from fusant of carotenogenic microalgae D. salina and C. vulgaris. Molecular characterization is needed for identifying the dominant parental genome in the fusant using ribosomal DNA sequences. The research was carried out by analyzing the gene encodes for 18S rDNA of fusant and determining relationship of fusant with D. salina and C. vulgaris species from GenBank. Quantitative analysis showed that C. vulgaris was not remarkably dominant in fusant with 84 % similarity compare to D. salina with 82 % similarity. The result indicated that the fusant gained both character from their parents.

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